Poster Session:PS 1130;Gastroenterology (Liver):A Sarcomatoid Carcinoma Arising from Intrahepatic Cholangiocyte: An Unusual Primary Liver Cancer

( Yo Han Kim ),( Kyoung Hwang Shin ),( Sung Hwahn Hahn ),( Jae Hwan Kong ),( Joon Ho Choi ),( Seoung Min Jeon ),( Hyun Deok Shin ),( Suk Bae Kim ),( Jung Eun Shin ),( Hong Ja Kim ),( Il Han Song ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Sarcomatoid carcinoma arising from intrahepatic cholangiocyte, an extremely rare primary liver cancer, has highly invasive and metastatic potential. The pathogenesis of this tumor is unclear, although histogenetic mechanisms such as ‘transformation’, ‘combination’ and ‘collision’ might be suggested to explain the simultaneous co-existence of carcinoma and sarcoma components in the same tumor. Case: A 58 year-old male presented with a hepatic mass that was found during regular health examination. Liver computed tomography (CT) scan revealed 2. 0 cm-sized heterogeneous low density mass without enhancement located on the segment 6. Magnetic resonance imaging showed the tumor of segment 6 with a low-signal intensity in T1-weighted image and a high-signal intensity in T2-weighted image. Positron emission tomography-CT showed a focal hypermetabolic lesion in the same area, with no evidence of distant metastasis. This mass was suspected to be intrahepatic cholangiocarcinoma by ultrasonography-guided needle biopsy, so right posterior sectionectomy was performed. The resected liver showed a well-defi ned whitish or yellowish gray solid mass with a central hemorrhage and necrosis at a subcapsular area of segment 6. Microscopically, the tumor tissue consists of multiple polygonal and pleomorphic cells. The tumor cells were immunohistochemically positive for cytokeratin 19 and vimentin, but negative for Hep-par 1. The patient was confi rmed a defi nitive diagnosis of intrahepatic sarcomatoid carcinoma. After 2 months, the patient developed extensive abdominal metastases and received systemic chemoradiotherapy, resulting in no response visible radiologically. Conclusions: We report an unusual case of sarcomatoid carcinoma of the liver arising from intrahepatic cholangiocyte, confi rmed by immunohistochemical analysis following surgical resection.

Sudden Death Associated With Possible Flare-Ups of Multiple Sclerosis After COVID-19 Vaccination and Infection: A Case Report and Literature Review

Jeon Yo Han,Choi Sangjoon,Park Ji Hyun,Lee Jong Kyu,Yeo Nam Seok,Lee SangHan,서연림 대한의학회 2023 Journal of Korean medical science Vol.38 No.10

We present an autopsy case of a 19-year-old man with a history of epilepsy whose unwitnessed sudden death occurred unexpectedly in the night. About 4 years before death, he was diagnosed with unilateral optic neuritis (ON). Demyelinating disease was suspected, but he was lost to follow up after the recovery. Six months before death, he received a second dose of mRNA coronavirus disease 2019 (COVID-19) vaccine. Three months before death, he experienced epileptic seizures for the first time. Seventeen days before death, he was infected with COVID-19, which showed self-limited course under home isolation. Several days before death, he complained of seizures again at night. Autopsy revealed multifocal gray-tan discoloration in the cerebrum. Histologically, the lesions consisted of active and inactive demyelinated plaques in the perivenous area of the white matter. Perivascular lymphocytic infiltration and microglial cell proliferation were observed in both white matter and cortex. The other major organs including heart and lung were unremarkable. Based on the antemortem history and postmortem findings, the cause of death was determined to be multiple sclerosis with suspected exacerbation. The direct or indirect involvement of cortex and deep gray matter by exacerbated multiple sclerosis may explain the occurrence of seizures. Considering the absence of other structural abnormalities except the inflammatory demyelination of the cerebrum, fatal arrhythmia or laryngospasm in the terminal epileptic seizure may explain his sudden unexpected death in the benign circumstances. In this case, the onset of seizure was preceded by COVID-19 vaccination, and the exacerbation of seizure was preceded by COVID-19 infection, respectively. Literature reporting first manifestation or relapse of multiple sclerosis temporally associated with COVID-19 vaccination or infection are reviewed.

Rubrofusarin-6-β-gentiobioside inhibits lipid accumulation and weight gain by regulating AMPK/mTOR signaling

Han, Yo-Han,Kee, Ji-Ye,Park, Seong-Hwan,Mun, Jeong-Geon,Jeon, Hee-Dong,Park, Jinbong,Zou, Qin-Peng,Liu, Xiang-Qian,Hong, Seung-Heon Elsevier 2019 Phytomedicine Vol.62 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Although rubrofusarin-6-β-gentiobioside (RFG), which is a component of <I>Cassiae tora</I> seed, could likely regulate hyperlipidemia, its anti-obesity effect and related mechanism have not been elucidated.</P> <P><B>Purpose</B></P> <P>The aim of this study was to examine whether RFG can ameliorate obesity and the mechanism of lipid accumulation regulated by RFG.</P> <P><B>Study design</B></P> <P>In <I>in vitro</I> experiments, we confirmed the anti-adipogenic effect of RFG using 3T3-L1 cells and human adipose mesenchymal stem cells (hAMSCs). To confirm the anti-obesity effect, High-Fat Diet (HFD)-induced obese mice were selected as a model.</P> <P><B>Methods</B></P> <P>We investigated anti-adipogenic effects of RFG using MTS assay, Oil Red O Staining, real-time RT-PCR, western blot analysis, and immunofluorescence staining. The anti-obesity effect of RFG was confirmed in HFD-induced mice model using hematoxylin and eosin staining and serum analysis.</P> <P><B>Results</B></P> <P>RFG inhibited lipid accumulation in 3T3-L1 cells and hAMSCs by reducing expression of mammalian targets of rapamycin (mTOR), peroxisome proliferator-activated receptor (PPAR)γ, and CCAAT-enhancer binding protein (C/EBP)α. RFG phosphorylated AMP-activated protein kinase (AMPK) in a liver kinase B (LKB) 1-independent manner. Moreover, the anti-adipogenic effect of RFG was blocked by AMPK inhibitor. These results suggest that RFG inhibits lipid accumulation via AMPK signaling. Furthermore, RFG reduced the body weight, size of epididymal white adipose tissue (eWAT), and fatty liver in the mice. RFG also suppressed levels of adipogenic factors PPARγ, C/EBPα, FAS, LPL, and aP2) by activating AMPK in the eWAT and liver.</P> <P><B>Conclusion</B></P> <P>RFG can ameliorate obesity, and thus, could be used as a therapeutic agent for treating obesity.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Antioxidant Effect of Gastro-Intestine Enzymatic Hydrolysates from Olive Flounder (Paralichthys olivaceus) Fed with Fermented Garlic Solution

Yo-Han Jang,Ji-Hyeok Lee,Jae-Young Oh,Ju-Young Ko,Yong-Seok Ahn,Chang-Ik Ko,You-Jin Jeon 한국식품영양과학회 2012 한국식품영양과학회 학술대회발표집 Vol.2012 No.10

여성요도에 발생한 평활근종 13례

전준영,심봉석,박영요,권성원,한운섭 梨花女子大學校 醫科大學 醫科學硏究所 1991 EMJ (Ewha medical journal) Vol.14 No.2

Benign urethral neoplasm, especially leiornyoma of the female urethra, is a very rare entity, with relatively few cases reported. Recently we experienced 3 cases of leiomyoma in the female urethra and so reported with review of the literatures.

Evaluation of the Attenuation Rate for Clinical Application of a Flexible Skin Dosimeter in Radiotherapy

Han Moo-Jae,Yang Seung-Woo,Shin Yo-Han,Bae Sang-Il,Moon Young-Min,Jeon Wan,Yu To-Sol,Kang Jin-Kyu,Kim Jin-Young,Heo Seung-Uk,박성광,나정은,Chung Jin Beom 한국물리학회 2020 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.77 No.11

During radiotherapy, some patients owing to the variability in skin sensitivities, may experience adverse skin reactions for doses at which other patients are asymptomatic. Hence, when abnormal symptoms appear on a patient's skin, the etiology should be verified. Accordingly, a surface dosimeter on the skin can help ascertain whether accurate doses are irradiated. Furthermore, surface doses are measured in the field junction gaps during craniospinal irradiation or during large-area radiotherapy to avoid adverse reactions due to cumulative skin doses from multiple beams. However, the point dose measurement errors of surface dosimeters exceed approximately 10% and have low-positional accuracies because the attachment sites are approximated with the help of the naked eye. Moreover, accurate information on field junction gaps cannot be obtained. Therefore, in clinical practice, a flexible array dosimeter is required as it can measure the dose distribution on the body surface by adapting its shape to the body curvatures. In this study, we implemented a flexible array dosimeter and, based on its constituent materials, evaluated the attenuation rate associated with its use. A polyester (PET) film was used as the underlying substrate. HgI2 and PbI2 photoconductors were used for their good performances in films. Furthermore, the measurement accuracy was verified through comparisons with Monte Carlo simulation outcomes. The resulting PET film had an attenuation rate of 2.22% at a 50-μm thickness. At the minimum material thickness of 25 μm, the attenuation rates for HgI2 and PbI2 were 4.69% and 5.72%, respectively. Further research is needed to fabricate HgI2-based digital skin dosimeters with a thickness under 25 μm. Radiation detection and pixel resolution should be evaluated to accurately confirm the field junction gap.

Inhibitory Effect of Metformin Therapy on the Incidence of Colorectal Advanced Adenomas in Patients With Diabetes

( Yo Han Kim ),( Ran Noh ),( Sun Young Cho ),( Seong Jun Park ),( Soung Min Jeon ),( Hyun Deok Shin ),( Suk Bae Kim ),( Jeong Eun Shin ) 대한장연구학회 2015 Intestinal Research Vol.13 No.2

Background/Aims: Metformin use has been associated with decreased colorectal cancer risk and mortality among diabetic patients. Recent research suggests that metformin use may decrease the incidence of colorectal adenomas in diabetic patients with previous colorectal cancer. This study aimed to assess the clinical effect of metformin use on the development of colorectal adenomas in diabetic patients without previous colorectal cancer. Methods: Among 604 consecutive diabetic patients who underwent colonoscopic surveillance after initial colonoscopy between January 2002 and June 2012, 240 patients without previous colorectal cancer were enrolled in this study and were divided in two groups: 151 patients receiving metformin and 89 patients not receiving metformin. Patient demographics and clinical characteristics as well as the colorectal adenoma incidence rate were retrospectively analyzed. Results: The incidence rate of total colorectal adenomas was not different according to metformin use (P=0.349). However, the advanced adenoma incidence rate was significantly lower in the metformin group compared with the non-metformin group (relative risk [RR], 0.09; P=0.011). Metformin use was independently associated with a decreased incidence of advanced colorectal adenomas after adjustment for clinically relevant factors (RR, 0.072; P=0.016). In addition, the cumulative development rate of advanced adenomas during follow-up was significantly lower in the metformin group compared with the non-metformin group (P=0.007). Conclusions: Metformin use in diabetic patients without previous colorectal cancer is associated with a lower risk of advanced colorectal adenomas. (Intest Res 2015;13:145-152)

Single dose pharmacokinetics of the novel transdermal donepezil patch in healthy volunteers

Kim, Yo Han,Choi, Hee Youn,Lim, Hyeong-Seok,Lee, Shi Hyang,Jeon, Hae Sun,Hong, Donghyun,Kim, Seong Su,Choi, Young Kweon,Bae, Kyun-Seop Dove Medical Press 2015 Drug design, development and therapy Vol.9 No.-

<P><B>Background</B></P><P>Donepezil is an acetylcholinesterase inhibitor indicated for Alzheimer’s disease. The aim of this randomized, single-blind, placebo-controlled, single-dose, dose-escalation study was to investigate the safety, tolerability, and pharmacokinetics of the donepezil patch in healthy male subjects.</P><P><B>Methods</B></P><P>Each healthy male subject received a single transdermal donepezil patch (72 hours patch-on periods) of 43.75 mg/12.5 cm<SUP>2</SUP>, 87.5 mg/25 cm<SUP>2</SUP>, or 175 mg/50 cm<SUP>2</SUP>. Serial blood samples were collected up to 312 hours after patch application. The plasma concentrations of donepezil were determined by using a validated liquid chromatography–tandem mass spectrometry method. Pharmacokinetic parameters were obtained by noncompartmental analysis. Tolerability of the patches and performance of the patches (adhesion, skin irritation, residual donepezil content in the patch) were assessed throughout the study.</P><P><B>Results</B></P><P>The study was completed by 36 healthy subjects. After patch application, the maximal plasma donepezil concentration (C<SUB>max</SUB>) and the area under the curve (AUC) increased in a dose-proportional manner. Median time to C<SUB>max</SUB> was ~74–76 hours (~2–4 hours after patch removal), and mean t<SUB>1/2β</SUB> was ~63.77–93.07 hours. The average donepezil residue in the patch after 72 hours was ~73.9%–86.7% of the loading dose. There were neither serious adverse events nor adverse events that lead to discontinuation. Skin adhesion of the patch was good in 97.2% of the subjects. All skin irritations after patch removal were mild and were resolved during the study period.</P><P><B>Conclusion</B></P><P>The donepezil patch appeared to be generally well tolerated and adhesive. Pharmacokinetic analysis of the donepezil patch demonstrated linear kinetics.</P>

이유자돈 사료 내 동애등에(Hermetia illucens )의 첨가수준이 사양성적, 영양소소화율 및 경제성에 미치는 영향

최요한(Yo-Han Choi),윤세영(Se-Yeong Yoon),전세민(Se-Min Jeon),이재영(Jae-Young Lee),오승민(Seung-Min Oh),이승형(Seung-Hyung Lee),김진수(Jin-Soo Kim) 한국산학기술학회 2019 한국산학기술학회논문지 Vol.20 No.9

본 시험은 이유자돈 사료 내 동애등에(Hermetia illucens )의 사용 수준에 따른 성장, 영양소소화율 및 경제성을 평가하여 이유자돈 사료 내 동애등에의 적정 사용수준을 도출하기 위해 실시하였다. 본 시험을 위해 총 192 두의 이유자돈(Landrace × Yorkshire × Duroc; 6.51 ± 0.15 kg)을 공시하여 4 처리 6 반복, 반복당 8 두씩 완전임의 배치하였다. 시험 처리구는 옥수수-대두박 기초사료를 대조구로 하여 기초사료 내 동애등에를 각각 1, 2 및 3% 첨가하였다. Phase Ⅰ, Ⅱ 및 overall의 사양성적에서 유의적인 차이가 나타나지 않았으나, overall의 일당증체량에서 동애등에 첨가수준에 따라 선형적으로 증가하는 것으로 나타났다(p < 0.05). 건물 소화율이 동애등에 첨가수준에 따라 유의적으로 증가하는 것으로 나타났으나, 다른 항목에서 유의적인 효과는 나타나지 않았다. 경제성 분석에서 overall의 총증체량이 동애등에의 첨가수준이 증가함에 따라 증가하였으며(p < 0.05), 이를 제외한 모든 항목에서 유의적인 효과가 나타나지 않았다. 본 연구결과를 요약하면, 사료 내 동애등에의 첨가는 경제성의 영향 없이 이유자돈의 성장과 건물 영양소소화율 개선에 유의한 효과를 미치며, 이는 이유자돈 사료 내 3%까지 사용이 가능한 것을 시사한다. This trial was conducted to determine the optimal supplementation level of Hermetia illucens (HI) on the growth performance, the apparent total tract retention of nutrients and the economic efficiency in weaning pigs. A total of 192 weaned pigs (Landrace × Yorkshire × Duroc; 6.51 ± 0.15 kg) were randomly allotted to 4 treatments and 6 replicates on the basis of their initial body weight. The dietary treatments included a corn-soybean meal based diet supplemented with 0, 1, 2 or 3% HI. There were no significantly effects between the treatments in phases Ⅰ, Ⅱ and overall. But average daily gain was significant and linearly increased when HI was added to the diet in the overall group. Although digestibility of dry matter (DM) was increased by adding HI, digestibility responses were not observed by supplying HI in phase Ⅰ. Also, all the parameters of economic efficiency were not significantly different according to the level of HI. The results showed that HI had beneficial effects on growth performance and digestibility of dry matter. However, there were no effects on economic efficiency by using HI in weaning pig diets.

수중폭발에 의한 원통형 배열센서의 구조 응답 및 안정성 해석

전수홍(Jeon, Soo-Hong),홍진숙(Hong, Chin-Suk),정의봉(Jeong, Weui-Bong),서희선(Seo, Hee-Seon),조요한(Cho, Yo-Han) 한국소음진동공학회 2012 한국소음진동공학회 논문집 Vol.22 No.1

This paper establishes a modeling and simulation procedure for structural response and reliability of a cylindrical array sensor on submarines under the shock generated by underwater explosion. The structural reliability of SONAR is important because the submarine could get out of combat ability by the structural damage of the SONAR upon explosion. A cylindrical array sensor was first modeled using the finite element method. Modal analysis was then performed for the check of the reliability of the modeling. The shock resistance simulations were performed for the responses to the structural shock waves and for the responses to the directly applied underwater shock waves, according to BV-043 and MIL-STD-901D, respectively. The stresses of the structure were evaluated with von-Mises scheme. Vulnerable regions were exposed through mapping the maximum stress to the structural model. Maximum stress of the SONAR was compared with the yield stress of the material to examine the structural reliability.