Pulmonary inflammation caused by silica dioxide nanoparticles in mice via TXNIP/NLRP3 signaling pathway

Je‑Oh Lim,Je‑Won Ko,Tae‑Yang Jung,Woong‑Il Kim,So‑Won Pak,In‑Sik Shin,Won‑Kee Yun,Hyoung‑Chin Kim,Jeong‑Doo Heo,Jong‑Choon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Silica dioxide nanoparticles (SiONPs) have been used for various medical applications, including therapeutics and imaging, and the use of SiONPs has increased gradually over the years. However, despite an increase in the use of SiONPs, not much is known about mechanism of action of SiONPs and their pulmonary toxicity. Objective The present study investigated the pulmonary toxicity of SiONPs and explored the underlying mechanism of action, primarily focusing on thioredoxin-interacting protein (TXNIP)/NOD-like receptor pyrin domain-containing 3 (NLRP3) in SiONPs-treated mice. We investigated the toxic effects of SiONPs in the lung of BALB/c mice administered 5, 10, and 20 mg/kg SiONPs for 3 days. Results Exposure to SiONPs markedly increased inflammatory cell counts, including those of neutrophils and macrophages, and levels of inflammatory mediators, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in a dose-dependent manner in the bronchoalveolar lavage fluid. Moreover, the inflammation was verified upon histopathological analysis. In addition, exposure to SiONPs increased the expression of TXNIP in a dose-dependent manner and, in turn, upregulated NLRP3 inflammasome proteins, which subsequently induced IL-1β production. Conclusion Collectively, exposure to SiONPs induced inflammation in the lungs of mice, which resulted in the activation of IL-1β production via the TXNIP-NLRP3 axis. Our results provide useful information on the pulmonary toxicity induced by SiONPs and provide insights into the underlying mechanism of action.

고속회전 하는 정밀부품을 위한 연질/경질 코팅의 트라이볼로지적 특성에 관한 연구

오진규,정구현,김대은,유제환,김형재 한국공작기계학회 2003 한국공작기계학회 춘계학술대회논문집 Vol.2003 No.-

Recently, rotating elements which use mechanical and electrical systems have been utilized for high speed and accuracy to increase the performance. The most important thing to get a more reliable system is to understand the friction, wear and characteristics which has an effect on various coated surfaces. In this study, the tribological characteristics of various soft/hard materials were investigated by using a custom-built pin-on-reciprocator tester. From the experimental results, it was found that the friction coefficients of the soft material coated surfaces were lower under various normal loads due to thier self-lubricating ability and material transfer to the counter surface.

랫드에서 신우황청심원의 급성 및 아급성독성시험

오승민,남혜윤,김준수,연제덕,신대희,이진영,박대규,조명행,정규혁 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

The acute and subacute toxicity of New Woohwangchungsimwon(NWCH) which was used l-muscone as substitutive material of musk were investigated in S.D. rats. In intraperitoneal acute toxicity test, rats(Sprague-Dawley, SPF) were injected intraperitoneally with dosages of 0, 540, 750, 1,070, 1,500 and 3,000 ㎎/㎏. Body weights were significantly decreased at 540 ㎎/㎏ dose group in both sexes and abnormal autopsy findings were founded in both sexes at all treated groups. Intraperitoneal LD_50 of NWCH was 812.3 ㎎/㎏ in male and 872.3 ㎎/㎏ in female rats. In the subacute toxicity study, NWCH was administrated orally to both sexes of rats for 4 weeks as several doses(0, 320, 800 and 2,000 ㎎/㎏). There were neither dead animals nor significant changes of body weights during the experimental period. In addition, no differences were found between control and treated groups in clinical signs, urinalysis, hematology, serum biochemical analysis, and other findings. Above data strongly suggest that no observed adverse effect level of NWCH might be over 2,000 ㎎/㎏/day in this study.

異所性 同種 補助肝 移植의 實驗的 硏究 (第一報)

吳在東 全南大醫大 1973 전남의대학술지 Vol.10 No.1

Pre-exposure에 依한 Pan-chromatic film의 超增感

吳濟雄 釜山工業大學校 1983 論文集 Vol.24 No.2

브레히트의 신즉물주의 수용과 극복

오제명 한국브레히트학회 1995 브레히트와 현대연극 Vol.2 No.-

In dieser Arbeit wurde beabsichtigt, durch die Analyse der fruheren theoretischen Schriften Brechts seinen Rezeptions- und Uberwindungs- prozeβ der 'Neuen Sachl ichkeit. zu erklaren, die in der Stabilisierungsphase der Weimarer Republik als der neue herrschende Zeitgeist auftrat. Es konnte festgestellt werden, daβ die neusachlichen Momente ab 1920 in betrachtlichen Maβe in den brechtschen Schriften ausgedruckt waren, besonders in seiner Expressionismuskritik, Filmkritik und seinem ersten Theaterkonzeption "Sport-Theater". Diese sind seine Abneigung gegen den Gefuhlsuberfluβ und die rhetorische Ubertreibung des Expressionismus, sein Willkommen des Films als neues Mediums und der Konsumenten-standpunkt des "Sport-Theaters". Wenn man Brechts Rezeption der 'Neuen Sachlichkeit' als eine unvermittelte annehmen wurde, dann wurde ihr Einfluβ auf ihn zu den sogenannten "Verdachten" gehoren. Aber seine Rezeption war im Grunde kritisch und souveran. Denn mit ihr teilte er nicht in der ersten Halfte der zwanziger Jahren die Ingenieur-Romantik, den Waren-Fetischismus und die kapitalistische Utopie, die wichtige Charakteristika der 'Neuen Sachlichkeit' sind. Die neusachliche Momente waren ihm vor allem die Angriffswaffe gegen das konventionelle burgerliche Theater und Publikum. Durch die Aneignung der marxistischen geschichtlichen Perspektive und der dialektischen Denkweise konnte Brecht ab 1926 schnell die 'Neue Sachlichkeit' uberwinden. Die obengenannten MIomente wurden fur den neuen Zweck dialektisch aufgehoben, d.h. aufbewahrt und negiert. Ende der zwanziger Jahren konnte Brecht diese Momente als Material zum Herstellen der vielfaltigen asthetischen Experimente verwenden.

유성견 정중구개봉합 확장후 봉합 재형성 과정에 대한 조직형태학 및 면역조직화학적 연구

오인섭,주성숙,신제원 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.1

Sutural regeneration requires the formation of new connective tissues comprised of various extracellular matrix components. The present study investigated the formation and distribution of the major ECM components such as fibronectin (FN), type I collagen (CI) during expanded sutural regeneration. The expansion were created on median palatine suture of young adult dog_ At 0 and 60 days after expansion, dogs were sacrificed by perfusion with 10% neutral buffered formalin The sutural area were cut, demineralized, dehydrated and embedded in paraffin. Immunostaining of FN and CI was achieved by the avidin-biotin complex method. The results were as follows 1. After expansion, much cellular infiltration was observed in the suture. The orientation of the fiber bundles become obscured. 2. At 60 days after expansion, the increase of the fibrous components were seen between both the sutural surfaces. The new bone formation were seen at the regenerated sutural surface. 3. After expansion, intensive staining for FN, CI was observed especially around blood vessels as well as inflammatory cells. This proteins also expressed later in the collagenous bundles. 4. At 60 days after expansion, intensive staining for FN and CI was observed in osteoid, newly formed connective tissue, and its attachment site to the sutural surface. 5. These results suggest that sutural regeneration is achieved through the cell differentiation and formation of Fh and CI by the cells.

제6차 교육과정에 의한 중학교 과학 교과서의 비교 분석 연구 : 중학교 1학년 과학 교과서를 중심으로 On the First Year of Middle School

오제직,홍춘표,김준식 공주대학교 사범대학 과학교육연구소 1996 과학교육연구 Vol.27 No.1

The purpose of this paper is to give basic data which could be used for re-construction textbooks according to real situation of school in teaching, and could help to write new textbooks. For this study, five kinds of Science textbooks Ⅰ on the 5th curriculum and eight kinds of science textbooks Ⅰ on the 6th curriculum were selected as an analytical material. The methods of study are as follows; We analyzed and compared respectively with Githic type, Figure, Table, Research, Question, Experiment, Observation, Exercise and so forth in eight kinds of Sience textbooks Ⅰ and in average values of five kinds of textbook on the 5th curriculum. 1. The kinds of the Science textbook based on the 6th curriculum and 5th curriculum were respectively 8 and 5. 2. The quantity of new textbooks were much the same as the old textbooks, except for C and H textbooks. 3. It was found out that new textbooks decreased more than old textbooks in number of page of theory and explanation. 4. The number of Gothic type term was showed more Chapter Ⅰ and Ⅱthan other chapter in old and new science textbooks. 5. Figure and Table have intrinsic character-respectively. 6. The number of Study and Figure were unchanged as compared with old textbooks. but its of Table were decreased. 7. The number of Question was also unchanged, but it had almost subjective. 8. The kinds and numbers of experiments were increased as compared with old textbooks, and they had instinctive character respectively. From these analytical results of new science textbooks, we, that is, science teachers should select science textbook well and we should supply a thing of insufficiecy on science textbook in teaching.

Bump露光量의 Control Method

吳濟雄 釜山工業大學校 1985 論文集 Vol.27 No.-

발치와의 치유과정에서 교원질 및 비교원단백질의 분포에 관한 면역조직화학적 연구

오화탁,김현수,주성숙,신제원 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

During tooth socket healing, the coagulum in the socket is replaced by fibrous connective tissue which undergoes mineralization and eventually becomes bone. Using this model, the healing process of the tooth socket and the role of type I collagen(CI), fibronectin(FN), bone sialoprotein(BSP), and osteopontin(OP) in the process were studied. Thirty Sprague-Dawley rats weighing 130-150gm were fed fi -aminopropionitcille for 5days before extraction of the first maxillary molars, and sacrified by perfusion with 4% paraformaldehyde at 1, 3, 5, 7, and 10days after tooth extraction. The socket and surrounding tissues were cut, demineralized, dehydrated and embedded in paraffin. For morphological observation, the sections were stained by Azan. Immunostaining of the ECM components was achieved by the avidin-biotin complex method. The results as follows; 1. Morphology of the tooth socket 1day after tooth extraction. The socket was filled with blood coagulum which was composed of densly aggregated red blood cells, platelets and fibrin network. 2. Morphology of the tooth socket 3days after tooth exyraction. A large portion of the coagulum was replaced by fibrous connective tissue contains a large number of fibroblasts, come from periphery of the socket. 3. At 5days, the newly formed bone demonstrated the highest proliferation activity. At 7days, the soclet was occupied with new bone. 4. Type I collagen was observed in the newly formed connective tissue and around the new bone. fiber. 5. Intensive staining for fibronectin was observed in granulation tissue, especially around blood vessels as well as inflammatory cells. 6. Strong immunostaining for bone sialoprotein and osteopontin was found in osteoblasts and new bone, while weaker staining was observed on dense connective tissue. 7. These data suggest that collagen and noncollagen proteins (FN, BSP, OP) an important role daring socket healing.