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      • 2019년도 2학기 신라대학교 재학생의 부산인식 설문조사 분석 : 인문사회계 학생을 대상으로

        김대래, 신군재, 유영명, 장희정, 정쾌영 신라대학교 부산학연구센터 2016 부산연구 Vol.17 No.2

        이 논문은 신라대학교에 재학 중인 학생들을 대상으로 부산에 대한 인식을 알아보기 위해 수행 한 설문지에 대한 분정확히 알아보기 위하여 분석에서는 부산출신자를 대상으로 하였다. 학생들과 부모의 출생연도와 연령 등에 관한 기초분석을 하고 부산의 자랑과 특성에 관한 인식을 분석 하였다. 아울러 학생들의 일석이다. 총 237부의 응답지를 받았는데 그 중에서 부산출생자 153명 을 대상으로 분석을 하였다. 부산에 관한 인식을 좀 더 상생활과 관련된 설문을 분석하였다. 분석에는 spss24를 이용하여 빈도분석과 기술통계분석 그리고 교차분석을 하였다.

      • 밸리댄스 참가자의 참가동기 요인 분석

        김장환,허은숙,엄대영 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

        This study aims to confirm the existence of participating motives of those who participate in bellydance in terms of demographic characteristics to examine the role of bellydance as leisure sports and the diferences in the motives according to the degree of participation, and to study the effective aspects of bellydaqnce as a way of sound leisure activities of modern people. A total of 200 questionnaire were distributed to those who participate in the bellydance programs of the Social Education Centers and Life Long Education Centers in Seoul, Daegu and Busan and 190 of them were collected, 177 questionnaires(93.2%), except those questionnaires whose answers were not sincere and some parts of which were missing, were analyzed positively. The data of this study were processed by using SPSs/PC+11.0.Cronbach's Alpha Coefficient was calculated for Frequency Analysis and Reliability Test to confirm general characteristics. And Principal Component Factor Analysis was conducted by using Verimax Orthogonal Rotation Factor Analysis to confirm the structure of motive factors ofr participation in bellydance. In order to confirm the differences between group and in the group by each factor. One-Way (ANOVA) was conducted. The findings obtained from the study model based on these methods of the study and the analysis of the dats are as follow: First, it is confirmed that there are significant differences in the participating motives by demographic characteristics. Second, it is confirmed that there are significant differences in the participating motives by the degrees of participation.

      • 대구광역시 생활체육 동호인조직의 활성화 방안

        엄대영,김장환,유재원 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.2

        This study has made a comparative analysis of the sport for all sports-lovers organizations of the advanced countries and those of Daegu Metropolitan City through the study of the literature using the previous studies and the examination of the literature and has aimed to present the ways to activate the sport for all sports-lovers organizations. The following conclusions have been drawn: First, it is necessary to strengthen the support by making the National Council of Sport for All a legal body. Second, it is necessary to put the systems in good order in terms of policies in order to elevate the concern about and the participation in sport for all sports. Third, it is necessary to make up for the weak points of the systems in limiting and restraining the events to support in the regulations of the extent of the events of the sport for all sports for the sake of the sport for all sports-lovers and for the creation of new clubs. Fourth, it is necessary to promote the regular exchanges between sports-lovers in order to strengthen the entities of the sports-lovers clubs, and it is important to activate the league systems by the events by putting together the sports-lovers clubs in the neighboring areas.

      • KCI등재후보

        석면폐증 1례

        이영수,장태원,유호대,정만홍,이용환,서지영,허 방,이재성 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.1

        Asbestosis is the disease of pulmonary fibrosis caused by the inhaled asbestos fibers, and could be diagnosed clinically, in the case of exposure history to asbestos is proved, by clinical symptoms of dyspnea or dry cough, physical examonation findings, and the radiographic features. But many othder inorganic dusts would show similar findings in the chest radiogram and sometimes the exposure history is obscure, so for the exact diagnosis of asbestosis lung biopsy is needed. In Korea, there have been some reports of survey in the workplace where asbestos is handled or of asbestos related diseases. This is a case report of asbestosis with accompanying pleural plaques, who had the occupational exposure to asbestos for 30 years and the consistent clinical, radiographic and pathological findings in the lung tissue obtained by the videoscope assisted thoracoscopic biopsy(VATS).

      • Numerical Calculation of Flame Characteristics in a LNG-Fired Combustor

        Jang, Dong-Soon,Kim, Yeong-Dae,Baek, Joong-Hyun 忠南大學校 環境問題硏究所 1992 環境硏究 Vol.10 No.-

        수치해석 방법을 이용하여 LNG를 사용한 산업용 규모의 연소로의 화염특성을 연구하였다. 특히 본 논문에서 중점을 둔 사항은 화염의 lift-off에 따른 폭발에 의한 안전문제 등 제반 여러문제와 관련된다. 수치해석적 방법은 검사체적에 기초한 유한 차분법을 사용하였으며 이 확산화염에서 연료(LNG)와 공기의 주입 속도 및 온도 등에 따른 lift-off 현상 및 기타 제반 화염 특성을 규명하였다. A non-equilibrium turbulent reaction model has been developed in order to describe the local flame extinction which is visualized by flame lift-off due to the dominance of turbulence mixing over chemistry in nonpremixed LNG-fired combustor. The model calculates the average nonequilibrium species Yi. A method was presented for calculating the mean chemical reaction term ωi, which appears in the equation of Yi, ∇ㆍ(ρuYi)=∇ㆍ(Γ∇Yi)+ωi In this method ωi is expressed as -1/2ρχd²Y/dξ², where χ,Yi, and ξ stand for the scalar dissipation rate, the instantaneous species mass fraction and the conserved scalar mixture fraction respectively. A rational choice for Yi(ξ) was made knowing that this distribution must lie between the corresponding equilibrium and frozen chemistry distribution. Futhermore flammable mixture range as linear functions of temperature was include so as to incorporate the upper and lower flammability, and thereby to determine the shape of the nonequilibrium species profiles. The mean reaction term can then be calculated by assuming the probability density distribution functions for χ and ξ. SIMPLER algorithm was used to resolve the pressure-velocity coupling problem together with the power-low scheme for discretization. Turbulence was closed using the κ-ε model with proper wall function treatment. Four-flux model was incorportated for radiation. Predicted results have been compared with the experimental data of Lewis and Smoot??. Predictions from the nonequilibrium model show good agreements with he data while the fast chemistry equilibrium calculations underestimate the centerline O₂ values, overestimate CO₂ values. In addition, the parametric calculations have been done in terms of the secondary air temperature which result in good agreement with experimental profiles.

      • KCI등재

        Interaction of GAT1 with Ubiquitin-Specific Protease Usp14 in Synaptic Terminal

        Dae-Hyun Seog(석대현),Sang-Jin Kim(김상진),Young-Ju Joung(정영주),Sung Su Yea(예성수),Yeong-Hong Park(박영홍),Moo Seong Kim(김무성),Il Soo Moon(문일수),Won Hee Jang(장원희) 한국생명과학회 2010 생명과학회지 Vol.20 No.7

        γ-aminobutyric acid (GABA)는 중추신경계에서 억제성으로 작용하는 주요한 신경전달물질이다. GABA 수송체(GAT)는 연접간격에 존재하는 GABA를 세포 내로 재 흡수하여 GABA의 농도를 조절한다. 그런데 GABA 수송체가 어떻게 조절되는지는 아직 밝혀지지 않았다. 본 연구에서는 효모 two-hybrid system을 사용하여 뇌의 주요 GABA 수송체인 GAT1의 C-말단과 특이적으로 결합하는 ubiquitin-specific protease 14 (Usp14)를 분리하였다. Usp14는 GABA 수송체 GAT1및 GAT2와는 결합하지만, 다른 GAT isoform과는 결합하지 않았다. GAT1과의 결합에는 Usp14의 C-말단부위가 필수적으로 관여함을 확인하였다. 또한 이 단백질간의 결합을 GST pull-down assay로 확인하였으며, 생쥐 뇌 균질액의 co-immunoprecipitation을 통하여 in vivo에서도 GAT1과 Usp14가 결합함을 확인하였다. 이러한 결과들은 Usp14가 GAT1과 결합하여 세포막에 존재하는 GAT1의 수를 조절하는 역할을 할 가능성을 시사한다. γ-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system. GABA transporters (GATs) control extracellular GABA levels by reuptake of released GABA from the synaptic cleft. However, how GATs are regulated has not yet been elucidated. Here, we used the yeast two-hybrid system to identify the specific binding protein(s) that interacts with the carboxyl (C)-terminal region of GAT1, the major isoform in the brain and find a specific interaction with the ubiquitin-specific protease 14 (Usp14), a deubiquitinating enzyme. Usp14 protein bound to the tail region of GAT1 and GAT2 but not to other GAT members in the yeast two-hybrid assay. The C-terminal region of Usp14 is essential for interaction with GAT1. In addition, these proteins showed specific interactions in the glutathione S-transferase (GST) pull-down assay. An antibody to GAT1 specifically co-immunoprecipitated Usp14 from mouse brain extracts. These results suggest that Usp14 may regulate the number of GAT1 at the cell surface.

      • SCISCIESCOPUS

        Nuclear Localization and Functional Characteristics of Voltage-gated Potassium Channel Kv1.3

        Jang, Soo Hwa,Byun, Jun Kyu,Jeon, Won-Il,Choi, Seon Young,Park, Jin,Lee, Bo Hyung,Yang, Ji Eun,Park, Jin Bong,O'Grady, Scott M.,Kim, Dae-Yong,Ryu, Pan Dong,Joo, Sang-Woo,Lee, So Yeong American Society for Biochemistry and Molecular Bi 2015 The Journal of biological chemistry Vol.290 No.20

        <P>It is widely known that ion channels are expressed in the plasma membrane. However, a few studies have suggested that several ion channels including voltage-gated K<SUP>+</SUP> (Kv) channels also exist in intracellular organelles where they are involved in the biochemical events associated with cell signaling. In the present study, Western blot analysis using fractionated protein clearly indicates that Kv1.3 channels are expressed in the nuclei of MCF7, A549, and SNU-484 cancer cells and human brain tissues. In addition, Kv1.3 is located in the plasma membrane and the nucleus of Jurkat T cells. Nuclear membrane hyperpolarization after treatment with margatoxin (MgTX), a specific blocker of Kv1.3 channels, provides evidence for functional channels at the nuclear membrane of A549 cells. MgTX-induced hyperpolarization is abolished in the nuclei of Kv1.3 silenced cells, and the effects of MgTX are dependent on the magnitude of the K<SUP>+</SUP> gradient across the nuclear membrane. Selective Kv1.3 blockers induce the phosphorylation of cAMP response element-binding protein (CREB) and c-Fos activation. Moreover, Kv1.3 is shown to form a complex with the upstream binding factor 1 in the nucleus. Chromatin immunoprecipitation assay reveals that Sp1 transcription factor is directly bound to the promoter region of the Kv1.3 gene, and the Sp1 regulates Kv1.3 expression in the nucleus of A549 cells. These results demonstrate that Kv1.3 channels are primarily localized in the nucleus of several types of cancer cells and human brain tissues where they are capable of regulating nuclear membrane potential and activation of transcription factors, such as phosphorylated CREB and c-Fos.</P>

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