최근 5년간 약진으로 입원한 환자의 고찰 - 임상양상 및 검사소견(1986∼1990) -

고우석,조광현,윤재일 大韓臨床藥理學會 1993 臨床藥理學會誌 Vol.1 No.1

한약수치에 관한 연구(제 9보) : 炒甘草 제법의 표준화 및 규격화(1) Preparing Standardization and Regulation of Stir-Frying Glycyrrhzia root(1)

최혁재,이우정,박성환,송보완,김동현,김남재 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

In this study, We carried out the preparing standardization and regulation of processed Glycyrrhizae Radix(PGR) which have been widely used in oriental medicines. Glycyrrhizae Radix(GR) have been generally prepared by the stir-frying, or mix-frying with honey for purpose of decreasing sweetness and augmenting vitality. Firstly, we tried to standardize PGR prepared by the stir-frying. We purchased 14 kinds of PGR and non-processed GR(NPGR) at oriental physician's offices and oriental pharmacies on a nation scale, respectively. The amounts of dry on loss, water extract, diluted ethanol extract, ether extract, total ash, acid insoluble ash, glycyrrhizin(GL), glycyrrhetic acid(GA) and liquiritin(LQ) of them were examined. The amounts of dray on loss, GL and LQ in commercial PGRs showed remarkable decrease, while GA showed increase as compared with NPGR. In order to standardize preparing method of PGR, the effect of heating time on physico-chemical parameters and biological activities were examined. Physico-chemical parameters such as dry on loss, extract amount, GL and LQ contents in PGRs showed decrease, however, GA was increased with heating time as compared with NPGR. Also, GA obtained form heat-treated GR, was found as an artifact in PGRs. PGR was more effective than NPGR in vitro test of DPPH scavenging effect and TBA-Rs reducing effect. PGR and NPGR showed potent hepatoprotective effect on CCl_(1)-intoxicated rats. Especially, PGR prepared by 80 min of heating was the most effective. Considering these results, the optimal condition for PGR preparation was 150℃ for 80min.

GC/NPD를 이용한 다성분 잔류농약의 동시분석

김우성,이선화,김상엽,정동윤,김재이,이영자,이홍재,정성욱,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.10

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with fiorisil, and finally analyzed simultaneously the organophosphorus pesticides using GC/NPD, Ultra-2 and Ultra-1 fused silica capillary columns were used to separate and identify the products. Recovery of most analytes from soybean sample, taken from pesticide residues well, was greater than(80%) for all except(6) analytes. This method can simultaneously determine multiple pesticides with a high degree of accuracy and precision.

Coir Roll을 이용한 자연형 호안의 수리학적 특성

宋在偶,李尙度 弘益大學校 科學技術硏究所 2001 科學技術硏究論文集 Vol.12 No.-

Laboratory flume experiments to investigate the hydraulic characteristics by using close-natured element "coir roll" are carried out. This study examines the comparision if mean velocity and roughness coefficient in the case of spread the coir roll and plant the vegetation on the coir roll. The experiments was conducted using a 0.9m-wide, 0.45-deep flume with 8m-length and a radius if 2.5m in 180℃ An approximately 5cm thickness coir roll spread the flume bottom, 10cm in length and a 0.5cm in diameter vegetation planted at intervals of 9cm on the coir roll. The water was recirculated throughout the flume and return curcuit by a centrifugal pump. The results show that spread the coir roll and plant the vegetation on the coir roll are smaller each 43 percent and 46 percent than not spread the coir roll in the case of mean velocity. In the case of roughness coefficient, spread the coir roll and plant the vegetation on the coir roll are larger each 2.54 times and 2.37 times than not spread the coir roll.

未計測 小河川流域의 計劃洪水量 算定에 관한 硏究

宋在偶,丁榕泰 弘益大學校 1988 弘大論叢 Vol.20 No.2

The objective of this paper was to find best method of the synthetic unit hydrograph arbitrary small watershed. The following results were analyzed from the four methods. 1) For the development of more accurate peak discharge, the size and shape of the watershed need be considered. 2) The peak discharge of SCS method rather than that of Nakayasu's method was affected by drainage area and time of concentration. this is due to watershed characteristics. 3) kajiyama's and Nakayasu's method leave something to be desired for the evaluation of the accurate design discharge.

척수종양의 임상적 관찰

송재욱,김수한,우정현 全南大醫大 1987 전남의대학술지 Vol.24 No.1

상향류식 嫌氣性 生物 反應器에서 有機性 廢水의 유기물 부하량에 따른 biogas 성상에 관한 연구

김재우 건국대학교 1992 대학원 학술논문집 Vol.35 No.-

The effect of hydraulic ratention time (HRT) on the upflow anaerobic sludge blanket has been studied in order to use the gas produced as an energe source when the HRT was descreased from 10.0day to 0.5day step by step with constant influent COD of 7000 mg/1, organic removal efficiency was gradually decreased down to the HRT of 0.7day, and at HRT less than 0.7day, digester operation was failed. In the experiment, the Cod removal rate was the direct function of origanic loading rate and maximum organic removal efficiency was 89.4%.

카드뮴 중독 흰쥐에 대한 Noninvasive Biomarker로서의 요중 Ascorbic Acid에 관한 연구

이용우,김용호,안승주,류재두 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1997 Journal of biomedical laboratory sciences Vol.3 No.2

본 연구는 카드뮴의 이용 및 환경오염의 증가로 일어날 수 있는 카드뮴 중독에 대한 새로운 생물학적 지표로서의 요중 ascorbic acid를 평가하기 위하여 실험적으로 중독시킨 Sprague-Dawley종의 흰쥐를 이용하여 요중 ascorbic acid측정, 신장의 조직병리학적 조사 및 생화학적 간 기능 검사를 실시하였다. 측정된 요중 ascorbic acid농도의 전 실험기간 (50일) 평균치는 실험군Ⅰ(카드뮴 100ppm)이 214.0㎎/dl, 실험군Ⅱ(카드뮴 200ppm)가 254.3㎎/dl로 대조군 9.0㎎/dl에 비해 각각 24배 및 28배의 증가를 보였다. 조직병리학적 소견으로는 신장의 근위 세뇨관에서 단백뇨의 원인으로 추정되는 호산성 초자양 물질이 관찰되어 신장의 손상이 있었고, 생화학적 분석에서 실험군Ⅰ에서 AST, ALT의 수치가 대조군의 143㎎/dl, 50㎎/dl에 비해 각각 199㎎/dl, 88㎎/dl, 실험군Ⅱ가 270㎎/dl,226㎎/dl로 나타나 간 기능의 손상이 있었음을 알 수 있었다. 이상의 결과로 실험동물이 카드뮴에 노출됨에 따라 그 아만성 독성으로서 간장 및 신장 기능의 손상이 있었고 또한 요중의 ascorbic acid가 현저하게 증가되어 요중 ascorbic acid가 실험동물의 카드뮴 노출에 대한 noninvasive진단 지표로서 활용될 수 있을 것으로 기대된다. This study was planned to evaluate the urinary ascorbic acid as a new bilolgical marker for the intoxication of cadmium, which could possibly be driven by its increased utilization and environmental pollution. In order to meet this goal, we have performed measurement of urinary ascorbic acid concentration, histopathological examination of the kidney, and biochemical test for the liver function using cadmium-intoxicated rats by oral administration. The average concentrations of urinary ascorbic acid in the CdCl₂-treated rats were 214.0㎎/dl for 100ppm group and 254.3㎎/dl for 200ppm group during experimental period of 50 days. These levels are 24 and 28 times higher than one in the control group (9.0㎎/dl), respectively. Ultrastructural study showed the eosinophilic hyaline cast and focal effacement, fusion in the renal tubules, as well as loss of foot processes on the glomerular epithelial cells. These results suggested that cadmium may be responsible for renal glomerular injury. The blood levels of AST, ALT and LDH in the treated groups (199 IU/I, 88 IU/I, 1190 U/I for the 100 ppm group and 270 IU/I, 226 IU/I, 760 U/I for the 200 ppm group) were higher than ones in the control group(143 IU/I, 50 IU/I, 334 U/I). These results indicated the cadmium induced the damage of liver function. In conclusion, the administration of cadmium showed a remarkable increase of urinary ascorbic acid with renal and hepatic damage. Therefore, it is expected that measurement of urinary ascorbic acid would be an powerful method as a noninvasive biomarker for cadmium intoxication.

비대칭 원환을 이용한 교각주위 세굴 방지 효과

宋在偶,沈遇培 弘益大學校 科學技術硏究所 1998 科學技術硏究論文集 Vol.9 No.1

Laboratory flume experiments to investigate the characteristics of the flow and local scour around circular shapes of bridge pier in a straight channel are performed. This study examines the effect of a asymmetry circular collar devices for controlling the depth of scour. As the ratio of asymmetry increased, the scour depth decreased. In addition, the maximum reduction of scouring is about 67% for CASE3 and CASE4. The prevention of scouring is most effective when the asymmetry collar for all case is placed at 0.1h below river bed.

GC-ECD를 이용한 유기염소계 및 Pyrethroid계 농약 동시 분석법

김우성,이선화,김재이,정지윤,이명자,박영채,이영자,정성욱,이봉헌,박흥재 한국환경과학회 2003 한국환경과학회지 Vol.12 No.4

Pesticides were extracted from samples with 70% acetone and methylene chloride in order, and then cleaned up via open-column chromatography apparatus packed with florisil, and finally analyzed simultaneously the organochlorine and pyrethroid pesticides using GC(ECD). An ultra-2 fused silica capillary column was used to separate and identify the products. The resolution between the last isomeric peak of cypermethrin(59.987min) and the first isomeric peak of flucythrinate(60.043min) was not satisfactory. The last isomeric peak of fenvalerate(62.344min) and the first isomeric peak of fluvalinate(62.397min) were overlapped. Recoveries of soybean sample for the most pesticides were 73.3% to 102.4%. Detection limits were between 0.004 and 0.063 ㎍/㎎ when this method was used.