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감마선조사 생쥐의 폐 및 심장조직중의 Superoxide Dimutase 활성도에 대한 연구
이상협,전현우,김우제,양종대,차종희 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1
The change of cytosolic superoxide dismutase (E.C.1.15.1.1. ) activities in the lung and heart of mice irradiated with a single dose of gamma irradiation 400 rads for the whole body was examined. The specific activities of superoxide dismutase in cytosol fraction of lung and heart were decreased to lowest levels on 7 day after irradiation and the activities of superoxide dismutase per gram of the organ tissues were decreased to lowest level on 21 day after irradiation. Also the total activities of superoxide dismutase per lung and heart were decreased to lowest level on 7 day after irradiation, It is concluded that the superoxide dismutase activity in cytosol of heart is more decreased than the activity in cytosol of lung after irradiation.
장희순,최병재,양호정,이승일 大韓小兒齒科學會 1996 大韓小兒齒科學會誌 Vol.23 No.2
As a part of host factors of dental caries,salliva has been well known for its important role in relation to dental caries.The studies on its physical and chemical charaterisitics on development and progress of dental caries has been condected.Recently,various comparisons between saliva of caries-susceptable individuals and caries-free individuals has been done and the efforts to understand the machanisms of salivary intervention of development and progress of dental caries is actively in progress.In this study,15 children with rampant dental caries and 15 caries free children without any systemic diseases from the ages of 2 to 5 were chosen for the experiment and the whole saliva and parotid saliva from each individuals were collected and protein compared using polyacrylamide gel electophyoresis(PAGE). As results of this study,in parotid saliva,there was no difference in protein compositions between the rampant dental caries and caries free children.While electrophoresis was done with the whole saliva,protein with 120 KDa was found in children with rampant dental caries.However,this protein was not found or unclear,if any for the caries free group.(Exceptionally,clear protein band was present for one person.)Protein compositions of whole saliva rampant dental caries group was compared before and after the caries control and thick and clear protein bands of about 120 KDa were found in both cases.Protein compositions of caries free children and adults were indentical.Quantitative analysis of protein was done for the rampant dental caries group and the control group and no significant difference was found. Taken all together,protein with molecular weight of 120 KDa,found in rampant dental caries group,was still present when the treatment for the dental caries was done so it can be assumed that this protein has no interrelation with the presence of active carious lesions during saliva collecting.It can also be presumed that this specific salivary protein with the molecular weight of 120 KDa found in rampant dental caries group has effect on development and progress of dental caries.Identification on this protein with the molecular-weight of 120 KDa and the role of this protein against dental caries remain to be solved.
박병윤,이부용,양소영,강상재,이동훈 대구효성가톨릭대학교 응용과학연구소 1997 응용과학연구논문집 Vol.6 No.1
본 연구에서는 전통적으로 우리 식탁의 주요 부식으로 이용되어온 콩나물과 미나리를 Cd와 Pb가 함유된 배양액으로 재배하는 동안 이들 채소에 Cd와 Pb 가 어느 정도 흡수되는가를 조사하였다. 또한 수경재배에서 Cd와 Pb에 의한 콩나물의 생육장해 정도를 조사하였다. 배양액속에 함유된 Pb이온은 재배기간 동안 미나리와 콩나물 체내로 쉽게 흡 수되었다. 그리고 미나리는 콩나물보다 더 많은 양의 Pb를 흡수하였는데, 이 는 미나리의 줄기에 다량의 뿌리가 붙어있기 때문인 것으로 생각된다. 또한 배양액속의 Pb의 농도와 미나리와 콩나물 체내에 축적된 Pb의 함량과는 높은 상관성을 나타내었는데, 각각의 상관계수(r^2)는 0.9411, 0.9828이었다. Cd역시 Pb와 마찬가지로 재배기간 동안 미나리와 콩나물 체내로 쉽게 흡수되 었다. 또한 배양액속의 Cd의 농도와 미나리와 콩나물 체내에 축적된 Cd의 함 량과는 높은 상관성을 나타내었는데, 각각의 상관계수(r^2)은 0.9900, 0.9054이었다. 그러나 두 식물체에서 Pb의 흡수 경우와는 달리 배양액속에 함유되어 있는 Cd의 농도가 높을 경우 콩나물이 미나리보다 더 많은 Cd을 흡수하였다. 콩나물의 경우에는 Cd과 Pb이 재배기간 동안 거의 비슷한 양으로 흡수되었으며, 그리고 배양액 속에 함유된 이들 중금속들과 콩나물 체내에 축적된 중금속들 사이의 직선회귀선의 기울기도 거의 유사하였다. 그러나 미나리의 경우에는 Pb이 Cd보다 상당히 많은 양이 흡수되었으며, 또한 직선회귀선의 기울기도 Cd보다 Pb의 경우 상당히 크게 나타났다. Cd와 Pb의 농도가 1mg/l일 경우에는 콩나물 길이의 성장 또는 외관상의 생육에 거의 영향이 없었다. 그러나 10mg/l 농도에서부터는 길이의 성장장해뿐만 아니라 외관상으로도 분명한 장해현상이 나타났다. 50mg/l와 100mg/l의 농도에서는 콩나물의 생육장해가 더욱 심해졌다. Pb의 경우에는 100mg/l의 농도에서, Cd의 경우에는 50mg/l의 농도에서부터 재배기간 동안 콩나물의 상당 부분이 썩는 현상이 나타났다. This study was conducted to investigate the uptake of Cd and Pb by vegetables. Bean sprout and dropwort, which are very important for traditional korean dishes, were cultivated in culture fluid added with Cd and Pb cations, and the content of Cd and Pb absorbed by these vegetables were measured. Pb cations in culture fluid were easily absorbed into bean sprout and dropwort. And dropwort absorbed Pb more than bean sprout did. Pb contents of bean sprout and dropwart dramatically increased with increasing Pb concentration in culture fluid. The correlation coefficients(r^2) between Pb contents in dropwort and bean sprout and Pb concentrations in culture fluid were 0.9411 and 0.9828, respectively. Cd cations were also easily absorbed into bean sprout and dropwort. The correlation coefficients(r^2) between Cd contents in dropwort and bean sprout and Cd concentrations in culture fluid were 0.9900 and 0.9054, respectively. The growth of bean sprout was not hindered at the concentration of 1mg/ℓ Cd and Pb in culture fluid. But high Cd and Pb concentrations hindeded remarkably the growth of this vegetable. A large part of bean sprout was rotten at the concentrations of 50 mg/ℓ Cd, and 100 mg/ℓ Pb.
경구면역을 통한 항원 특이적 IgA 항체 합성에 있어 Cholera Toxin과 Alginate-Microsphere의 효과
송민형,유진수,권명상,성승룡,김용희,권익찬,정서영,양재명,김평현 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.3
Secretory (S-IgA) isotype antibody (Ab) is known to play an essential role in the primary defense against various infectious agents in mucosal tissue. However, it has been mostly unsuccessful in the induction of antigen (Ag)-specific IgA Ab response in this site by peroral vaccination. In the present study, we investigated the effect of cholera toxin (CI) as a mucosal adjuvant and alginates-microspheres as a carrier on BSA-specific IgA Ab response in gut-associated lymphoid tissue`(GALT). Peroral immunization of BSA plus CT conferred a great BSA-specific IgA response but IgG response on intestinal fluid (IF). In contrast, intraperitoneal immunization of BSA with Freund's adjuvant readily induced BSA-specific IgG response but IgA response in IF. Further, number of CT specific IgA-secreting cells was substantially increased in mesenteric lymph node when CT-encapsulated-VI alginates-microspheres was administered perorally. Taken together, these results indicate that peroral immunization of soluble antigen in combination of CT or microspheres significantly enhances antigen-specific IgA response in GALT.
(Hoon Jai Chun),(Dong Kyu Park),(Chul Hee Park),(Jae Hong Park),(Yoon Tae Jeen),(Soon Ho Um),(Sang Wo Lee),(Jai Hyun Choi),(Chang Duck Kim),(Ho Sang Ryu),(Jin Hai Hyun),(Yang Seok Chae),(Chang Sub Uhm 대한내과학회 2002 The Korean Journal of Internal Medicine Vol.17 No.1
N/A Background : The adhesion of H. pylori to the gastric epithelial cells may be an essential step for the pathophysiology of various H. pylori-induced gastrointestinal diseases. The purpose of this study was to investigate the ultrastructural relation of H. pylori and gastric epithelial cells in their adhesion. Methods : Endoscopic biopsy of gastric antrum and body was performed from 15 patients (9 men, 6 women) with chronic gastritis and H. pylori infection. The specimens were processed for electron microscopy and observed with a transmission electron microscope (Hitachi H-600). Results : On the basis of morphological appearances, the adhesions of H. pylori to the gastric epithelial cells were categorized into three types, filamentous connection, adhesion pedestals and membrane fusion. Coccoid and undetermined forms adhered mainly by the filamentous connection, whereas the bacillary forms adhered primarily by the adhesion pedestals and membrane fusion. Conclusion : Various types of adhesion were associated with H. pylori and gastric epithelium. Further studies are needed to evaluate the influence of different types of adhesion to the pathophysiology of H. pylori.
SOFC 밀봉재로서 SiO₂-R₂O₃-RO계에서 R₂O₃, RO 변화 따른 특성
양상진(Yang, Sang-Jin),최병현(Choi, Byung-Hyun),이미재(Lee, Mi-Jai),지미정(Jee, Mi-Jung),이홍림(Lee, Hong-Lim),유영성(Yoo, Yung-Sung) 한국신재생에너지학회 2007 한국신재생에너지학회 학술대회논문집 Vol.2007 No.11
SOFC 스택 밀봉재로서 SiO₂-R₂O₃계를 선정하여 R₂O₃와 RO를 변화 하였을 때 유리 전이 온도, 열팽창계수, 이종 물질간의 젖음성, 환원 가스에 반응성 등을 조사하였다. 또한 모유리에 filler를 넣었을 때 filler 첨가에 따른 특성을 조사한 결과 B₂O₃/SiO₂ 함량비 증가에 따라 열팽창계수는 증가하였고 전이점은 낮아졌으며 밀봉재를 이용한 SUS 와 SUS를 800?C 접합 후 산화 {cdot} 환원분위기에서 작동했을 때. 화학적 반응 및 확산 반응층은 관찰되지 않았다.