Antiviral Peptide Engineering: From Chemical Design Principles to Therapeutic Applications

( Joshua A. Jackman ) 한국공업화학회 2021 한국공업화학회 연구논문 초록집 Vol.2021 No.-

Nurse Practitioner Preceptorship Model

Shirley Strachan-Jackman 한국간호과학회 2009 한국간호과학회 학술대회 Vol.2009 No.10

Colloid-inspired nanoplasmonic biosensing strategies for antiviral peptide evaluation

( Joshua A. Jackman ) 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-

Surface-based nanoplasmonic sensors are powerful tools to characterize biomacromolecular interactions with biomembrane-mimicking platforms. In this talk, I will discuss how nanoparticle- and nanohole-based nanoplasmonic sensing platforms can be utilized to characterize the mechanistic properties of a membrane-active, antiviral peptide. Three examples will be covered: (i) peptide-induced rupture of a close-packed lipid vesicle adlayer on titanium oxide-coated gold nanodisks; (ii) peptide-induced rupture of individual virus-like particles within long-range-ordered, polymer-functionalized gold nanoholes; and (iii) peptide-induced disruption of positively curved membranes in short-range-ordered, silica-coated gold nanoholes.

Polymer-Coated Metallic Nanoholes for Capturing Biological Nanoparticles

Joshua A. Jackman 한국고분자학회 2019 한국고분자학회 학술대회 연구논문 초록집 Vol.44 No.1

Supported lipid membrane platform technology for anti-infective applications

윤보경,문수지,( Joshua A. Jackman ) 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-

We demonstrate that adding mixtures of lauric acid (LA) and glycerol monolaurate (GML) - two of the most biologically active antimicrobial fatty acids and monoglycerides - to a supported lipid bilayer platform triggers concurrent tubule and bud formation, which unexpectedly results in synergistic phospholipid membrane remodeling that far exceeds the effects of GML or LA alone. The most pronounced effects occurred with equimolar concentrations of GML and LA in the micellar state, highlighting that synergistic membrane disruption and an inability to relieve membrane strains. These findings offer a new model to explain how fatty acid and monoglyceride interactions can trigger phospholipid membrane remodeling events relevant to various biophysical and biological systems.

Temperature-Induced Denaturation of BSA Protein Molecules for Improved Surface Passivation Coatings

Park, Jae Hyeon,Jackman, Joshua A.,Ferhan, Abdul Rahim,Ma, Gamaliel Junren,Yoon, Bo Kyeong,Cho, Nam-Joon American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.38

<P>Bovine serum albumin (BSA) is the most widely used protein for surface passivation applications, although it has relatively weak, nonsticky interactions with hydrophilic surfaces such as silica-based materials. Herein, we report a simple and versatile method to increase the stickiness of BSA protein molecules adsorbing onto silica surfaces, resulting in up to a 10-fold improvement in blocking efficiency against serum biofouling. Circular dichroism spectroscopy, dynamic light scattering, and nanoparticle tracking analysis showed that temperature-induced denaturation of BSA proteins in bulk solution resulted in irreversible unfolding and protein oligomerization, thereby converting weakly adhesive protein monomers into a more adhesive oligomeric form. The heat-treated, denatured BSA oligomers remained stable after cooling. Room-temperature quartz crystal microbalance-dissipation and localized surface plasmon resonance experiments revealed that denatured BSA oligomers adsorbed more quickly and in larger mass quantities onto silica surfaces than native BSA monomers. We also determined that the larger surface contact area of denatured BSA oligomers is an important factor contributing to their more adhesive character. Importantly, denatured BSA oligomers were a superior passivating agent to inhibit biofouling on silica surfaces and also improved Western blot application performance. Taken together, the findings demonstrate how temperature-induced denaturation of BSA protein molecules can lead to improved protein-based coatings for surface passivation applications.</P> [FIG OMISSION]</BR>

Nanoplasmonic sensors for detecting circulating cancer biomarkers

Ferhan, Abdul Rahim,Jackman, Joshua A.,Park, Jae Hyeon,Cho, Nam-Joon,Kim, Dong-Hwan Elsevier 2018 Advanced drug delivery reviews Vol.125 No.-

<P><B>Abstract</B></P> <P>The detection of cancer biomarkers represents an important aspect of cancer diagnosis and prognosis. Recently, the concept of liquid biopsy has been introduced whereby diagnosis and prognosis are performed by means of analyzing biological fluids obtained from patients to detect and quantify circulating cancer biomarkers. Unlike conventional biopsy whereby primary tumor cells are analyzed, liquid biopsy enables the detection of a wide variety of circulating cancer biomarkers, including microRNA (miRNA), circulating tumor DNA (ctDNA), proteins, exosomes and circulating tumor cells (CTCs). Among the various techniques that have been developed to detect circulating cancer biomarkers, nanoplasmonic sensors represent a promising measurement approach due to high sensitivity and specificity as well as ease of instrumentation and operation. In this review, we discuss the relevance and applicability of three different categories of nanoplasmonic sensing techniques, namely surface plasmon resonance (SPR), localized surface plasmon resonance (LSPR) and surface-enhanced Raman scattering (SERS), for the detection of different classes of circulating cancer biomarkers.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Characterizing How Acidic pH Conditions Affect the Membrane-Disruptive Activities of Lauric Acid and Glycerol Monolaurate

Valle-Gonzá,lez, Elba R.,Jackman, Joshua A.,Yoon, Bo Kyeong,Park, Soohyun,Sut, Tun Naw,Cho, Nam-Joon American Chemical Society 2018 Langmuir Vol.34 No.45

<P>Fatty acids and monoglycerides are single-chain lipid amphiphiles that interact with phospholipid membranes as part of various biological activities. For example, they can exhibit membrane-disruptive behavior against microbial pathogens on the human skin surface. Supported lipid bilayers (SLBs) provide a useful experimental platform to characterize these membrane-disruptive behaviors, although related studies have been limited to neutral pH conditions. Herein, we investigated how lauric acid (LA) and glycerol monolaurate (GML) interact with SLBs and cause membrane morphological changes under acidic pH conditions that are representative of the human skin surface. Although LA induces tubule formation under neutral pH conditions, we discovered that LA causes membrane phase separation under acidic pH conditions. By contrast, GML induced membrane budding in both pH environments, although there was more extensive membrane remodeling under acidic pH conditions. We discuss these findings in the context of how solution pH affects the ionization states and micellar aggregation properties of LA and GML as well as its effect on the bending stiffness of lipid bilayers. Collectively, the findings demonstrate that solution pH plays an important role in modulating the interaction of fatty acids and monoglycerides with phospholipid membranes, and hence influences the scope and potency of their membrane-disruptive activities.</P> [FIG OMISSION]</BR>

Nanoarchitectonics-based model membrane platforms for probing membrane-disruptive interactions of odd-chain antimicrobial lipids

Yoon Bo Kyeong,TAN SUE WOON,Tan Jia Ying Brenda,Jackman Joshua A.,Cho Nam-Joon 나노기술연구협의회 2022 Nano Convergence Vol.9 No.48

The use of nanoscience tools to investigate how antimicrobial lipids disrupt phospholipid membranes has greatly advanced molecular-level biophysical understanding and opened the door to new application possibilities. Until now, relevant studies have focused on even-chain antimicrobial lipids while there remains an outstanding need to investigate the membrane-disruptive properties of odd-chain antimicrobial lipids that are known to be highly biologically active. Herein, using the quartz crystal microbalance-dissipation (QCM-D) and electrochemical impedance spectroscopy (EIS) techniques, we investigated how an 11-carbon, saturated fatty acid and its corresponding monoglyceride—termed undecanoic acid and monoundecanoin, respectively—disrupt membrane-mimicking phospholipid bilayers with different nanoarchitectures. QCM-D tracking revealed that undecanoic acid and monoundecanoin caused membrane tubulation and budding from supported lipid bilayers, respectively, and were only active above their experimentally determined critical micelle concentration (CMC) values. Monoundecanoin was more potent due to a lower CMC and electrochemical impedance spectroscopy (EIS) characterization demonstrated that monoundecanoin caused irreversible membrane disruption of a tethered lipid bilayer platform at sufficiently high compound concentrations, whereas undecanoic acid only induced transient membrane disruption. This integrated biophysical approach also led us to identify that the tested 11-carbon antimicrobial lipids cause more extensive membrane disruption than their respective 12-carbon analogues at 2 × CMC, which suggests that they could be promising molecular components within next-generation antimicrobial nanomedicine strategies.

Optimal formation of uniform-phase supported lipid bilayers from phospholipid–monoglyceride bicellar mixtures

Tun Naw Sut,Soohyun Park,Bo Kyeong Yoon,Joshua A. Jackman,조남준 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.88 No.-

Supported lipid bilayers (SLBs) spanning hydrophilic surfaces are industrially attractive biomimeticcoatings that mimic critical aspects of lipid membrane interfaces and are increasingly used inapplications spanning medicine, biotechnology, and environmental science. The use of adsorbing bicellelipid nanostructures composed of long- and short-chain phospholipid mixtures is an effective selfassemblydriven process for streamlined SLB fabrication. However, existing studies use synthetic shortchainphospholipids as a necessary bicelle component and such materials are not practical for industrialapplications. Herein, we investigated optimal conditions to fabricate SLBs from bicelles containing anindustrially useful monoglyceride called monocaprin (MC) in place of short-chain phospholipids. Theratio of long-chain phospholipid to MC along with total lipid concentration were systematically tested. Quartz crystal microbalance-dissipation (QCM-D) and time-lapsefluorescence microscopy experimentswere performed to characterize bicelle adsorption onto silicon dioxide surfaces, andfluorescencerecovery after photobleaching (FRAP) measurements were conducted to evaluate lateral lipid diffusionwithin the fabricated lipid adlayers. Depending on bicelle parameters, high-quality SLB formation withuniform phase properties was achieved and optimal ranges are described to ensure target performanceoutcomes without phase separation. Together, ourfindings demonstrate that MC-containing bicelles areuseful tools to form high-quality SLBs suitable for surface coating and biosensing applications.