Induction of bone formation by <i>Escherichia coli</i>‐expressed recombinant human bone morphogenetic protein‐2 using block‐type macroporous biphasic calcium phosphate in orthotopic and ectopic rat models

Park, J‐,C.,So, S‐,S.,Jung, I‐,H.,Yun, J‐,H.,Choi, S‐,H.,Cho, K‐,S.,Kim, C‐,S. Blackwell Publishing Ltd 2011 Journal of periodontal research Vol.46 No.6

<P><I>Park J‐C, So S‐S, Jung I‐H, Yun J‐H, Choi S‐H, Cho K‐S, Kim C‐S. Induction of bone formation by</I> Escherichia coli<I>‐expressed recombinant human bone morphogenetic protein‐2 using block‐type macroporous biphasic calcium phosphate in orthotopic and ectopic rat models. J Periodont Res 2011; 46: 682–690. © 2011 John Wiley & Sons A/S</I></P><P><B>Background and Objective: </B> The potential of the <I>Escherichia coli</I>‐expressed recombinant human bone morphogenetic protein‐2 (ErhBMP‐2) to support new bone formation/maturation using a block‐type of macroporous biphasic calcium phosphate (bMBCP) carrier was evaluated in an orthotopic and ectopic rat model.</P><P><B>Material and Methods: </B> Critical‐size (Φ 8 mm) calvarial defects and subcutaneous pockets in 32 Sprague–Dawley rats received implants of rhBMP‐2 (2.5 μg) in a bMBCP carrier or bMBCP alone (control). Implant sites were evaluated using histological and histometric analysis following 2‐ and 8‐wk healing intervals (eight animals/group/interval).</P><P><B>Results: </B> ErhBMP‐2/bMBCP supported significantly greater bone formation at 2 and 8 wk (10.8% and 25.4%, respectively) than the control at 2 and 8 wk (5.3% and 14.0%, respectively) in calvarial defects (<I>p</I> < 0.01). Bone formation was only observed for the ErhBMP‐2/bMBCP ectopic sites and was significantly greater at 8 wk (7.5%) than at 2 wk (4.5%) (<I>p</I> < 0.01). Appositional and endochondral bone formation was usually associated with a significant increase in fatty marrow at 8 wk. The bMBCP carrier showed no evidence of bioresorption.</P><P><B>Conclusion: </B> ErhBMP‐2/bMBCP induced significant bone formation in both calvarial and ectopic sites. Further study appears to be required to evaluate the relevance of the bMBCP carrier.</P>

솔 - 젤법으로 제조한 Pd 계 삼원촉매 반응에서 Ceria 의 영향

소현수,이진만,노점임,양오봉,김도희,우성일 한국공업화학회 1998 응용화학 Vol.2 No.2

The effect of ceria on the catalytic performance of Pd-only three way catalysts prepared by sol-gel method was investigated by XRD, XPS and synthetic exhaust gas reaction with GHSV of 72,000 h^(-1). Optimum Ce:Zr mole ratio to stabilize ceria against thermal aging and enhance the catalytic performance was 7:3. It was found that the physical mixing of Pd catalyst, ceria stabilized with zirconium et al. and bulk ceria showed significant improvement in three way catalytic performance and thermal stability. Thermal stability of the catalysts prepared by sol-gel method were more superior than that of the catalysts prepared by impregnation method.

Decomposition of 1,4-dioxane by photo-Fenton oxidation coupled with activated sludge in a polyester manufacturing process

So, M. H.,Han, J. S.,Han, T. H.,Seo, J. W.,Kim, C. G. IWA Publishing 2009 Water Science & Technology Vol.59 No.5

<P>The cyclic ether 1,4-dioxane is a synthetic industrial chemical that is used as a solvent in producing paints and lacquers. The EPA and the International Agency for Research on Cancer(IARC) classified 1,4-dioxane as a GROUP B2(probable human) carcinogen. 1,4-dioxane is also produced as a by-product during the manufacture of polyester. In this research, a polyester manufacturing company (i.e. K Co.) in Gumi, Korea was investigated regarding the release of high concentrations of 1,4-dioxane (about 600 mg/L) and whether treatment prior to release should occur to meet with the level of the regulation standard (e.g., 5 mg/L in 2010). A 10 ton/day pilot-scale treatment system using photo-Fenton oxidation was able to remove approximately 90% of 1,4-dioxane under the conditions that concentrations of 2800 ppm H2O2 and 1,400 ppm FeSO4 were maintained along with 10 UV-C lamps (240 μW/cm2) installed and operated continuously during aeration. However, the effluent concentration of 1,4-dioxane was still high at about 60 mg/L where TOC concentration in the effluent had been moreover increased due to decomposed products such as aldehydes and organic acids. Thus, further investigation is needed to see whether the bench scale (reactor volume, 8.9 L) of activated sludge could facilitate the decomposition of 1,4-dioxane and their by-products (i.e., TOC). As a result, 1,4-dioxane in the effluent has been decreased as low as 0.5 mg/L. The optimal conditions for the activated sludge process that were obtained are as follows: DO, 3-3.5 mg/L; HRT, 24 h; SRT 15 d; MLSS, 3,000 mg/L. Consequently, photo-Fenton oxidation coupled with activated sludge can make it possible to efficiently decompose 1,4-dioxane to keep up with that of the regulation standard.</P>

Optimization of biological wastewater treatment conditions for 1,4-dioxane decomposition in polyester manufacturing processes

Han, J. S.,So, M. H.,Kim, C. G. IWA Publishing 2009 Water Science & Technology Vol.59 No.5

<P>The solvent stabilizer 1,4-dioxane could have harmful effects on an ecosystem. The discharge limit of 1,4-dioxane in a body of water will be regulated at 5 mg/L in Republic of Korea. Thus, the currently operating activated sludge used in the manufacture of polyester should be properly treated to meet the regulations. Accordingly, the removal rate of 1,4-dioxane and its microbial properties was assessed at K, H and T corporations. The highest removal efficiencies were recorded at H. However, the concentration of 1,4-dioxane in the effluent of T exceeded the criterion. In addition, a microbial degradation test was conducted on 100 mg/L of 1,4-dioxane inoculated with the activated sludge from each of the three corporations. After 7 days, the 1,4-dioxane was completely removed with the H sludge and efficiencies were 67% in the T sludge and 52% in the K sludge. These results confirm that the biodegradability of 1,4-dioxane may vary in relation to the microbial properties. The microbial diversity of activated sludge of each company was therefore investigated by 16S rDNA cloning methods. In conclusion, the activated sludge of H is the most effective for the biodegradation of 1,4-dioxane. This fact is of significant concern for the industrial sector.</P>

Bioremediation of Cd by Microbially Induced Calcite Precipitation

Kang, C. H.,Han, S. H.,Shin, Y.,Oh, S. J.,So, J. S. HUMANA PRESS INC 2014 Applied biochemistry and biotechnology Vol.172 No.4

Contamination by Cd is a significant environmental problem. Therefore, we examined Cd removal from an environmental perspective. Ureolysis-driven calcium carbonate precipitation has been proposed for use in geotechnical engineering for soil remediation applications. In this study, 55 calcite-forming bacterial strains were newly isolated from various environments. Biomineralization of Cd by calcite-forming bacteria was investigated in laboratory-scale experiments. A simple method was developed to determine the effectiveness of microbially induced calcite precipitation (MICP). Using this method, we determined the effectiveness of biomineralization for retarding the flow of crystal violet through a 25-mL column. When the selected bacteria were analyzed using an inductively coupled plasma optical emission spectrometer, high removal rates (99.95 %) of Cd were observed following incubation for 48 h. Samples of solids that formed in the reaction vessels were examined using a scanning electron microscope. The CdCO3 compounds primarily showed a spherical shape. The results of this study demonstrate that MICP-based sequestration of soluble heavy metals via coprecipitation with calcite may be useful for toxic heavy metal bioremediation.

Effect of ultrasonic treatment on swine wastewater solubilization

Lee, Y. H.,Kim, S.-M.,Na, S.,So, K.-H.,Nam, J.-J. IWA Publishing 2009 Water Science & Technology Vol.59 No.3

<P>In order to accelerate hydrolysis known to be the rate-limiting step of the overall digestion process for swine wastewater, an ultrasonic treatment process was tested for the solubilization of the swine wastewater. The effectiveness of ultrasonic solubilization of the swine wastewater under various operational conditions was compared by means of an increment of soluble organics in the treated swine wastewater and the hydrolysis rate constant. Ultrasonic treatment resulted in the high degree of solubilization of particulate organics in the swine wastewater and the degree of solubilization increased with increasing supplied energy. The highest extent of an increment of SCOD concentration and SCOD/TCOD ratio at the end of the operation time of 60 min was 109.7 and 117.5%, respectively, under 120 W power output and 20oC operating temperature conditions. The observed highest hydrolysis rate constant described by pseudo-first order rate constant was 2.94 h−1 under the same conditions. Based on the estimated activation energy from modeling using the Arrhenius equation, ultrasonic solubilization of the swine wastewater under higher supplied energy conditions was more dependent on the operating temperature, which was consistent with the experimentally obtained results. Based on the investigation into the effect of gas type and gas delivery methods for ultrasonic solubilization of the swine wastewater, oxygen gas bubbling through the liquid showed the highest degree of an increment of soluble organics possibly attributed to the influent of oxygen in an increase of radicals during the sonolysis.</P>

Empirical analysis of high pressure SF⁶ gas breakdown strength in a spark gap switch

Nam, S.H.,Rahaman, H.,Heo, H.,Park, S.S.,Shin, J.W.,So, J.H.,Jang, W. IEEE 2009 IEEE transactions on dielectrics and electrical in Vol.16 No.4

<P>It is difficult to predict or measure the breakdown strength of SF<SUB>6</SUB> gas in the high pressure range. Therefore, it is hard to find reference data of the high pressure SF<SUB>6</SUB> gas breakdown strength. This paper attempts to provide empirically a novel criterion for the estimation of SF<SUB>6</SUB> gas breakdown strength in a spark gap switch. For this purpose, J.C. Martin's breakdown field strength of non-uniform gap in air and Kuffel et al's breakdown field strength by exploiting streamer criterion of uniform field in SF<SUB>6</SUB> gas are adopted. The research is made for the empirical method of predicting the high pressure SF<SUB>6</SUB> breakdown strength and compared results have been ascertained with several experimental data from different investigators in a wide range of pd (pressure times distance) and field enhancement factor (FEF). For further confirmation of the empirical approach, we have designed and developed a high pressure spark gap switch to be closely coupled to a high voltage PFN-Marx system. The designed spark gap switch operates above 1 MV. Our tests have verified the prediction of the breakdown strength of the SF<SUB>6</SUB> filled spark gap switch in a pd range of 0.27-1.62 MPa . cm (2.7 to 16.2 bar.cm).</P>

Subregular Points for Some Cases of Lie Algebras

Kim, Y . K .,Choi, S . H .,Jeong, J . W .,So, K . H .,Park, D . Y . 호남수학회 1999 호남수학학술지 Vol.21 No.1

Dimensions of irreducible so_5(F)-modules aver an algebraically closed field F of characteristic p$gt;2 shall be obtained. It turns out that they should be coincident with p^m, where 2m is the dimension of coadjoint orbits of χ∈so_5(F)%* \ 0 as Premet asserted. But there is no subregular point for g=sp_4(F)=so_5(F) over F.

중년여성의 주관적인 건강상태, 갱년기 증상, 스트레스 대처행위가 중년 위기감에 미치는 영향

구다슬,김기현,소정은,이애현,이유정,이윤서,이희진,정서윤,Kawazoe Serina,강숙정,김윤정 이화여자대학교 간호학회 2018 이화간호학회지 Vol.- No.52

Purpose: This study aims to identify the effects of subjective health status, climacteric symptoms and coping behaviors of middle-aged women on mid-life crisis, and to provide basic data for developing nursing intervention to reduce mid-life crisis of the middle-aged women. Method: Surveys were distributed to middle-aged women between age of 40 to 65. 261 surveys were collected and analyzed using descriptive stastics, t-test, one-way ANOVA and Pearsson's correlation using the SPSS 20.0 program. Result: The mid-life crisis is affected by subject health status(β = -0.22, p < 0.001) and climacteric symptoms(β = 0.22, p < 0.001). It implies that in order for middle-aged women to have less mid life crisis, good subjective health status with less climacteric symptoms is required. Conclusion: The result of the study suggests that mid-life crisis of middle-aged women is related to subjective health status and climacteric symptoms. Therefore, in order to reduce mid-life crisis of middle-aged women, they should be provided climacteric education program to alleviate the severity of climacteric symptoms and nursing intervention to improve subjective health status.

Practices and predictors of 2009 H1N1 vaccination in cancer patients: a nationwide survey in Korea.

Shin, Dong W,Kim, Yeol,Park, Jong H,Cho, Juhee,Jho, Hyun J,Yang, Hyung-Kook,Kim, Hyun S,Kim, So Y Blackwell Publishing Ltd 2012 Influenza and other respiratory viruses Vol.6 No.6

<P>Because patients with cancer are considered to be at high-risk for influenza infection and related complications, annual vaccination is recommended. The emergence of the novel H1N1 influenza virus in 2009 complicated the medical care of patients with cancer. The present study examined H1N1 vaccination practices among patients with cancer during the pandemic season and investigated factors related to the vaccination.</P>