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웹 서비스 기반의 지방자치단체 민원서비스 통합방안에 관한 연구
강임철,김용일 호남대학교 2004 호남대학교 학술논문집 Vol.25 No.2
As information technology is prevailing, the integration of business processes has been expanded from private to public sector. Korean government introduced e-government and began to provide civil services electronically. However, current system is neither efficient nor convenient enough because the system is not supporting one-stop portal service. We suggest a model of the integrated electronic civil service using web service technology that can offer service integration under the distributed environment, also implement a prototype system for managing application to display placards.
The Relationship between Mitochondrial ROS and ER Stress in Allergic Airway Diseases
( So Ri Kim ),( Yong Chul Lee ),( Dong Im Kim ),( Mi Ran Kang ),( Yang Keun Rhee ),( Heung Bum Lee ),( Seoung Ju Park ),( Chi Ryang Chung ),( Seung Yong Park ),( Hee Jung Kim ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
Mitochondria and the Nox family of NADPH oxidase are the two major sources of reactive oxygen species (ROS) that are induced by external stimuli, and the mitochondria respiratory chain is considered as an important site of ROS production within most cells. Recent evidence has demonstrated that various biological stimuli increase accumulation of unfolded or mis-folded proteins in ER lumen, which is referred to as "ER stress". Moreover, these various pathologic stimuli have been reported to provoke oxidative stress as well as ER stress. In this study, we used the mice sensitized with OVA and LPS and then challenged with OVA (OVALPS-OVA mice) for elucidation of the relationship between mitochondrial ROS and ER stress in bronchial asthma. The OVALPS-OVA mice showed that the expression of ER stress markers and the protein levels of un-folded-protein response (UPR)-related marker in lung tissues were significantly increased after OVA challenge. In addition, we visualized the localization of mitochondrial ROS in BAL cells isolated from OVALPS-OVA mice using confocal microscopy; the significant increase in mitochondrial ROS in BAL cells was observed after OVA challenge. Our results also showed that Necrox-5 or 4-PBA significantly reduced the increases in ER stress, mitochondrial ROS, inflammatory cytokines, airway in-flammation, and bronchial hyperresponsiveness. These findings suggest that mitochondrial ROS and ER stress plays an im-portant role in the induction and maintaining allergic airway diseases synergistically.
( So Ri Kim ),( Yong Chul Lee ),( Dong Im Kim ),( Yang Keun Rhee ),( Heung Bum Lee ),( Seoung Ju Park ),( Chi Ryang Chung ),( Seung Yong Park ),( Mi Ran Kang ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-
Oxidative stress is well known to be implicated in the development of asthma. The mitochondrial respiratory chain is a major site of intracellular reactive oxygen species (ROS) generation and, at the same time, an important target for the damaging effects of ROS. Mito-Tempo is a specific mitochondrial ROS inhibitor and it is known to be associated with opening of mi-tochondrial permeability transition pore and inhibition of cell necroptosis or apoptosis. However, there is little information on the protective effects of Mito-Tempo on the inflammatory airway disorders including bronchial asthma and its acute exacerbation. We investigate the effects of Mito-tempo on the allergic airway inflammation and hyperresponsiveness using the mice sensitized with OVA and LPS and then challenged with OVA (OVALPS-OVA mice). The OVALPS-OVA mice showed the typical features of neutrophilic asthma; increased airway inflammatory cells, the pathologic changes, the increased levels of Th2 cytokines in lungs of OVALPS-OVA mice, increased mitochondrial ROS generation, and increased bronchial hyperresponsiveness. Interestingly, we found that in OVALPS-OVA mice, Mito-Tempo, a novel mitochondrial targeting agent significantly reduced the increases in inflammatory cytokines, mitochondrial ROS generation, airway inflammation, and bron-chial hyperresponsiveness. These findings indicate that mitochondrial dysfunction including oxidative damage may be im-plicated in the pathogenesis of bronchial asthma and provide the therapeutic potential of a mitochondrial targeting agent, Mito-Tempo, for bronchial asthma.
Kang, Eun-Sung,Kim, Chae-Young,Kim, Seon-Beom,Im, Se-Jin,Yang, Se-Hwan,Sung, Young-Chul,Kim, Byong-Moon 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.3
This study investigated the pharmacokinetic profile and biodistribution of HB-110, a novel HBV therapeutic vaccine candidate, in mice. HB-110 was rapidly degraded in the blood after i.v.injection with a half-life of 1.9${\pm}$0.083 min, and was no longer detected at 60 min except in one individual near the detection limit. In the i.m. injection, plasmid DNA was detectable at the injection site until ff days after administration, but the amounts were just above the detection limit. The blood concentration of HB-110 showed a maximum of 604 pg/mL at 15 min after i.m.injection, which was followed by degradation to undetectable levels at 90 min. The plasmid DNA in tissues peaked at 90 min after administration. The highest concentration of plasmid DNA was detected in the liver (24.172 pg/mg tissue), and considerable amounts were also observed in the lung (9.467 pg/mg tissue) and spleen (7.688 pg/mg tissue). The amount of plasmid DNA in tissues was 2 to 3 orders of magnitude lower than in the injection site at the same time points. The HB-110 concentration in tissues, including gonads, decreased rapidly and was undetectable 24 h after administration.
임종철,홍석우,강연익 釜山大學校生産技術硏究所 1998 生産技術硏究所論文集 Vol.55 No.-
최근 산업발달에 의한 교통량의 증가로 도로건설이 활발하게 진행되고 있는 실정이며, 도로건설은 지반조건이 불리한 연약지반상에서의 건설도 필수적이 되었다. 그러나, 연약지반에서의 성토공사시 시공상황을 적절히 고려하여 성토사면의 안정문제를 판단할 수 있는 전반적인 유지관리체계가 확립되어 있지않기 때문에 역약지반의 거동을 해석하기 위한 모델 및 시공시의 안정관리를 위한 연구가 활발히 진행되고 있다. 본 연구에서는, 설계 및 시공시 공정계획에 따른 성토사면의 안정성을 판단할 수 있도록 하기 위하여 정규압밀상태인 연약점토지반상의 점증성토와 방치기간을 고려할 수 있고, 압밀경과시간과 점토지반의 깊이에 따른 강도증가율을 고려할 수 있는 안정해석 프로그램(RSI-SLOPE)을 개발하였다. 이 프로그램을 이용하여 임의의 해석단면에 대하여 성토설계를 실시하였고 성토설계시 압밀계수(c)와 배수거리(H)의 영향을 비교·분석하였다. Recently, roads are being constructed actively due to traffic increase by industrial development. So, it is essential for the road to be constructed on the soft ground. But, it is not established to calculate stability of embankment with respect to the construction condition on soft ground. In this study, stability analysis program(RSI-SLOPE) is developed. By using this program, it is possible to consider the rate of strength increase according to consolidation period and the depth of clay ground as well as resting duration and gradually increasing embankment on the soft clay in normally consolidated state in order to be able to judge stability of embankment with time schedule in design or construction. In addition, this study contains analysis and comparison about influence of coefficient of consolidation(c) and drainage distance(H) in embankment design.
Growth of Budding Yeasts under Optical Trap
Im, Kang-Bin,Kim, Hyun-Ik,Kim, Soo-Ki,Kim, Chul-Geun,Oh, Cha-Hwan,Song, Seok-Ho,Kim, Pill-Soo The Korean Society of Toxicogenomics and Toxicopro 2007 Molecular & cellular toxicology Vol.3 No.1
Optic tweezer is powerful tool to investigate biologic cells. Of eukaryotic cells, it was poorly documented regarding to optic trapping to manipulate yeasts. In preliminary experiment to explore yeast biology, interferometric optical tweezers was exploited to trap and manipulate budding yeasts. Successfully, several budding yeasts are trapped simultaneously. We found that the budding direction of the daughter cell was almost outward and the daughter cell surrounded by other yeasts grows slowly or fail to grow. Thus it was assumed that neighboring cells around budding yeast may be critical in budding and the growth of daughter cells. This is first report pertaining to the pattern of yeast budding under the optical trap when multiple yeasts were trapped.
혈액질환치료에 있어 동종말초조혈모세포와 배양확장된 간엽모세포(Mesenchymal Stem Cell)의 동시이식 2예
강석윤,김재홍,박준성,김현수,최진혁,임호영,양말숙,김영진,김효철 대한조혈모세포이식학회 2001 대한조혈모세포이식학회지 Vol.6 No.1
Human bone marrow contain two kinds of stem cells. One is hematopoietic stem cell, and the other is multipotential mesenchymal stem cells which are capable of differentiating into a number of mesenchymal cell lineages. These mesenchymal stem cells are shown to secrete hematopoietic cytokines and support hematopietic progenitors in vitro. Animal models suggest that the transplantation of healthy stromal elements, including mesenchymal stem cells, may enhance the ability of the bone marrow microenvironment to support hematopoiesis after stem cell transplantation. Therefore, We hypothesized that cotransplantation of hematopoietic stem cells and mesenchymal stem cells after high dose chemotherapy would facilitate engraftment of hematopietic stem cells and reduce complications caused by delayed engraftments. And we investigated the safety, feasibility, side effects and hematopoietic effects of in vitro-expanded mesenchymal stem cells in hematologic malignancies receiving allogeneic peripheral stem cell transplantation.
Im, Hyung-Jun,Kim, Yong-Il,Paeng, Jin-Chul,Chung, June-Key,Kang, Soon-Beom,Lee, Dong-Soo The Korea Society of Nuclear Medicine 2012 핵의학 분자영상 Vol.46 No.1
Purpose : Retrocrural lymph nodes (RCLNs) communicate with retroperitoneal and posterior mediastinal LNs. It is possible that, when RCLNs are involved, supra-diaphragmatic extension will occur in abdomino-pelvic cancers. The authors investigated performance of $^{18}F$-FDG PET/CT to diagnose RCLN metastasis and whether RCLN metastases were associated with supra-diaphragmatic lymphatic metastases of ovarian cancer. Materials and methods : Sixty-seven patients with stage IV ovarian cancer who had undergone $^{18}F$-FDG PET/CT were included in this retrospective study. Diagnostic performance of $^{18}F$-FDG PET/CT for RCLN metastasis was evaluated. Patients were divided into two groups by presence or absence of supra-diaphragmatic LN metastasis. The prevalences of RCLN metastasis between the two groups were compared and the odds ratio was calculated. Results : Sensitivity and specificity of $^{18}F$-FDG PET/CT for RCLN metastasis were 96.3 and 100%, respectively. Of the 67 study subjects, 27 patients had RCLN metastases (40.3%). Fifty patients had supra-diaphragmatic LN metastases. $^{18}F$-FDG PET/CT showed 26 RCLN metastases in patients with supra-diaphragmatic LN metastases (54.5%), and only 1 in patients without supra-diaphragmatic LN metastasis (5.9%), and the difference between two groups was statistically significant (P<0.05). The odds ratio that patients with RCLN metastasis would have supra-diaphragmatic LN metastasis was 17.3 (95% confidence interval=2.1 to 140.9, P=0.008). Conclusion : Performance of $^{18}F$-FDG PET/CT to diagnose RCLN metastasis was excellent. RCLN metastasis revealed by $^{18}F$-FDG PET/CT was strongly associated with supra-diaphragmatic LN spread of ovarian cancer. Thus, RCLN metastasis could be used as a predictor of supra-diaphragmatic lymphatic metastasis of ovarian cancer.