OMC-2010 추출물이 마우스의 비장세포 cytokine 생성에 미치는 영향

배기상 ( Gi Sang Bae ),박경철 ( Kyoung Chel Park ),최선복 ( Sun Bok Choi ),조일주 ( Il Joo Jo ),서상완 ( Sang Wan Seo ),김종진 ( Jong Jin Kim ),신용국 ( Yong Kook Shin ),김민선 ( Min Sun Kim ),박규환 ( Kyu Hwan Park ),김현식 ( Hyu 대한본초학회 2012 大韓本草學會誌 Vol.27 No.5

Objective : This study was performed to estimate the effects of OMC-2010 extract on cytokine production in mouse spleen cells. Methods : Mouse spleen cells were pre-treated with ethanol and water extract of OMC-2010 for 1 h, then stimulated with lipopolysaccharide (LPS, 1 μg/ml) for 48 h. Then the cells were harvested for real-time reverse transcription polymerase chain reaction to detect cytokines. Results : OMC-2010 ethanol extract significantly inhibited the LPS-induced interleukin (IL)-1beta, tumor necrosis factor (TNF)-alpha and IL-5 mRNA expressions, but not shown such changes in IL-6, IL-4, IL-13. OMC-2010 water extract significantly inhibited the LPS-induced TNF-alpha, and IL-5 mRNA expressions, but not shown such changes in IL-1beta, IL-6, IL-4, IL-13. Conclusions : Theses results could suggest that both ethanol and water OMC-2010 extract could inhibit the TNF-alpha and IL-5 mRNA expression.

생쥐의 B 세포에서 anti-CD40과 rIL-4로 유도된 싸이토카인 생산에 대한 자오가의 효과

성일창 ( Il Chang Sung ),김형환 ( Hyung Hwan Kim ),안덕균 ( Duk Kyun Ahn ),이용섭 ( Yong Sup Lee ),서영배 ( Young Bae Seo ),최호영 ( Ho Young Choi ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.2

N/A Objectives : In order to study the anti-allegy effect of water extract of Acanthopanacis senticosi Radix (ASR) on the B-cells from healthy Balblc mice. Methods : The cytotoxicity of ASR was measured with the murine normal lung fibroblast cells by modified SRB assay. And the murine splenic B-cells was stimulated with anti-CD40 mAb and rIL4. The various cytokines related with allergy were measured by flow-cytometry and by RT-PCR with electophoresis. Results : The anti-allegy effects to ASR were identified and observed. The cytotoxicity of ASR on mouse lung fibroblast cells showed no significant activities. ASR had inhibitory effect on CD23+, CD69+, and IgE expression by ASR with anti-CD40 mAb plus rL-4-stimulated murine splenic B-cells. ASR had inhibitory effect on cytokines (E-lb, IL-4, IL-6, IL-10, TNF-a, TGF-81, INF-Y) and transcript expression and IgE production by ASR with anti-CD40 mAb plus rIL-4-stimulated murine splenic B-cells. Conclusion : We concluded that ASR showed anti-allegy effect on murine splenic B-cells.

가시오가피가 흰쥐의 Collagen 유발 관절염 발생 및 치료에 미치는 영향

오충환 ( Chung Hwan Oh ),김순중 ( Sun Jung Kim ),서일복 ( Il Bok Seo ) 한방재활의학과학회 2005 한방재활의학과학회지 Vol.15 No.3

Objectives : This study was carried out to investigated the anti-pathogenetic and curative effects of Radix Cortex Acanthopanacis Senticosis (CAS) on collagen-induced arthritis in rats. Methods : In experiment Ⅰ, rats(n=30) were divided into normal group, porcine type II collagen immunized control group which taken with normal saline, and collagen immunized treated group which taken with extract of CAS. Body weight, paw edema volume, and thickness of ankle joint were measured at 0, 10, 15 days after immunization. At 15 days after immunization, serum TNF-α, IL-1β and anti-collagen IgG level were analysed, and histopathological examination was performed on the ankle joint. In experiment Ⅱ, rats(n=13) that were shown the gross lesions of collagen-induced arthritis, were divided into control group and treated group which taken with extract of CAS for 15 days after onset of arthritis. Items and methods of measurement were the same as experiment Ⅰ. Results : In experiment I, the results were as follows ; Arthritic score, paw edema volume, thickness of ankle joint, TNF-α, anti-collagen IgG of treated group were significantly decreased. In experiment Ⅱ, the results were as follows ; Paw edema volume, TNF-α, IL-1β, anti-collagen IgG of treated group were significantly decreased. Conclusions : These results indicated that CAS has the inhibitory effects on the incidence and progression of collagen-induced arthritis in rats.

삼황사심탕(三黃瀉心湯)이 난황 알부민으로 유도된 알레르기 Mouse모델에서 항알레르기 효과

최종환 ( Chong Hwan Choi ),금선오 ( Seo Oh Keum ),이세원 ( Se Won Lee ),김일현 ( Il Hyun Kim ),이하일 ( Ha Il Lee ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2014 한방재활의학과학회지 Vol.24 No.3

Objectives In this study, we investigated the inhibitory effects of Samhwangsasim-tang (S.H) on the allergic response caused by ovalbumin(OVA) sensitization and challenge in BALB/c mice. Methods The experimental animals were divided into five groups; 1) normal as negative control, 2) OVA-sensitized mice, 3) OVA-sensitized mice treated with 200 mg/kg of S.H 200, 4) OVA-sensitized mice treated with 400 mg/kg of S.H 400, and 5) OVA-sensitized mice treated with 5 mg/kg of Dexamethasone (Dex). Antigen sensitization for allergic mouse model was performed with twice injection of OVA for 2 weeks. After secondary injection, S.H was administrated orally into mice every day for 13 days and the inhibitory effect of S.H on allergic responses was evaluated. Results Treatment of S.H into allergic mice reduced significantly ear edema and infiltration of immune cells in ear tissues induced with OVA challenge in a dose-dependent manner. S.H reduced significantly the serum levels of Total Immunoglobulin(Ig)G and IgE, and particularly inhibited the production of OVA-specific IgE, but not OVA-specific IgG. The serum level of proinflammatory cytokine TNF-α and Th2-associated cytokine IL-4 also were significantly decreased by S.H adminstration in a dose denpendent manner. S.H attenuated OVA-induced secretion of IFN-γ, but not IL-12 which is a cytokine inducing the development of Th1 cells. It also reduced significantly the secretion of IL-4, which is a cytokine inducing the development of Th2 cells, after splenocytes were stimulated with OVA. However the secretion of IL-5 and IL-13 was influenced weakly or a little. Conclusions These results indicate that S.H could reduce the allergic response through inhibition of antigen-specific IgE and Th2-inducing cytokines. It suggest that S.H may be available clinically for the treatment of allergic patients. (J Korean Med Rehab 2014;24(3): 71-85)

사람의 자궁 내막 조직내에서 Phosphodiesterase IV Inhibitor에 의한 IL-12의 조절 및 이에 따른 Th-1, Th-2 cytokine 분비 양상의 변화

박원일 ( Park Won Il ),김은경 ( Kim Eun Gyeong ),고덕성 ( Go Deog Seong ),홍서유 ( Hong Seo Yu ),나중열 ( Na Jung Yeol ),김대운 ( Kim Dae Un ),신정환 ( Sin Jeong Hwan ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.8

목적 : 사람의 초기 임신 과정에서 phosphodiesterase type IV inhibitor인 rolipram이 탈락막내 IL-12를 억제하고 이에 따라 Th-1 계열의 cytokine이 감소하고 Th-2 cytokine이 증가하는지를 규명하는 것이 목적이다. 연구 방법 : 임신 12주 이전에 계류 유산으로 진단받은 10명과 정상임신에서 임신 중절 수술을 시행받은 10명에서 자궁 소파술을 통하여 탈락막 조직을 획득한 후 조직을 rolipram으로 Objective : To assess the capability of phosphodiesterase type IV inhibitor (rolipram) to suppress IL-12 in human decidua and the subsequent changes of Th-2 cytokine (IL-10) and Th-1 cytokine (TNF-α). Methods : Decidual tissues of 10 first-trimester pregn

Aspergillus flavus에 의한 Versicolorin C의 생합성

서일원,조성환 慶尙大學校 1988 論文集 Vol.27 No.2

Potato dextrose agar상에 배양. 보관중인 Aspergillus flavus 를 Reddy's synthetic high -salts medium에 배양하고 균체로 부터 효소를 추출 분리한다음, ³H-Versicolorin A로 부터 화학 반을을거쳐 합성한 ³H-Versicolorin A hemiacetal을 기질로 하여 invitro 효소 반응을 시켰다.효소반응 결과 얻어진 반응생성 물질을 Thin layer Chromatography법으로 분리하고 표준화합물을 이용하여 비교동정하는 한편, 각 반응산물의 radiochromatogram를 작성하고, 추출. 분리한 화합물의 mass spctrometry 분석 결과, mass No 297,311,325,340에서 Peak를 가지는 Versicolorin의 존재를 확인. 동정할 수 있었다. 균체의 효소반응 실험결과, 조효소 무첨가구에서 (대조구에서)9.2%, NADH첨가구에서 10.3%,NADH첨가구에서 13.4%로 Versicolorin A hemiacetal이 Versicolorin C로 전환하는 것을 알 수 있어,NADPH가 이 환원반응을 촉진하는 reductase의 조효소작용에 관여함을 추정할 수 있었다. 따라서 포화 ammonium sulfate 용액으로 침전시켜 DEAE.Tris acryl M column chromatography에 의하여 정제, 분리한 효소 versicolorin A hemiacetal reductase의 spectrophotometric assay 결과에서도 NADH첨가구의 경우,2.67nmole/min의 효소 활성도를 관찰할 수 있어 Aspergillus flavus에 의한 versicolorin C의 생합성 기작을 확인할 수 있었다. Versicolorin A hemiacetal was converted to versicolorin Cin crude extracts and purified preparations from Aspergillus flavus. NADPH was required in the cell-free system. The rate of VAOH-dependent oxidation of NADPH was 0.43 mole/min/mg.protein. The substrate wa reduced in the cell-free system to a polar compound, proposed to be the open chain form. The polar compound was converted to the final product, versicolorin C.

김치 발효중 생체활성 아민 생성에 관한 연구

서일원,류춘선,이주돈,이영환,변광의 순천향대학교 기초과학연구소 1997 순천향자연과학연구 논문집 Vol.3 No.2

Biogenic amines can be formed and degraded either as the result of endogenous amino acid decarboxylase activity in raw materials or by the growth of decarboxylase-positive microorgannisms under conditions favorable to enzyme activity. Three biogenic amines(Tryptamine, Histamine, Tyramine) were intensively investigated by the HPLC during Kimchi fermentation at 5℃. The patterns of microfloral changes in lactic acid bacterial group, Leuconostoc, Streptococcus and Lactobacillus, were similar to the typical low-temperature fermentation. The population of microbial growth were high after 10 days and gradually decreased. The levels of Tyramine were lowered(10mg/kg) during this mid-periods(8∼16 days) but very high in initial-(15mg/kg) and final-periods (30mg/kg), in which the total acidity ranged between 0.2∼0.35% and 0.4∼0.6%, while the acidity in mid-periods revealed 0.35∼0.4% beginning the initial ripening period. Leuconostoc was highly increased in number in mid-period but slightly declined as the acidity increased, whereas Streptococcus was highly distributed in initial-period and Lactobacillus in final-period. The levels of Tryptamine and Histamine were relatively low throughout the whole fermentation period, but the profile of Tryptamine level was very similar to the case of Tyramine. Histamine was maintained at a certain level after mid-period of fermentation.

천연항균성 물질을 이용한 Vibrio vulnificus의 살균 및 독소생성 억제효과

조성환,서일원,최종덕,전상수,라택균,정수근,강동훈 國立統營水産專門大學 附設 水産科學硏究所 1993 수산과학연구소보고 Vol.4 No.-

생선회용 해산물에 오염되어, 생식한 경우 발생되는 패혈병의 원인균주인 Vibrio vulnificus의 생육 및 독성생성을 억제할 목적으로 천연 항균제인 Grapefruit종자추출물(GPSE)을 이용하여 그 항균효과를 검토하였다. In vitro 시험결과, Vibrio vulnificus에 대한 GFSE의 생육최소저해농도는 50~100 ppm 정도이었으며, Vibrio vulnificus 균체세포틀 100 ppm 농도의 GFSE 용액으로 처리하고 전자현미경 촬영시료로 조제하여 촬영한 후, 미생물형태을 무처리대조구와 비교, 검토한 결과, GFSE 처리로 세포막 기능이 파괴되어 세포내용물이 균체외부로 유출되어 균체의 생육이 억제되고 균체내부가 빈 ghost 형태의 균체수가 증대되어 GFSE의 항균효과를 확인할 수 있었다. 한편, 생선회용 해산물에 GFSE를 근육주사하고 Vibrio vulnificus를 접종한 수조내에서 사육하여 채취한 어육부를 마쇄하여 일정량씩을 표준사료에 첨가해서 통닭용 병아리를 실험동물로 하여 사양실험을 실시한 결과. 체중증가율, 단백질이용효율, 혈청내 효소활성, 혈청의 주요성분함량 등에 있어서, GFSE 처리수조내에서 사육한 해산물을 첨가한 시료시험구의 경우, 무첨가사료처리구와 비교할 때 뚜렷한 차이를 목격할 수 없었으며, GFSE 첨가에 따른 실험동물의 성장저해 또는 독성현상을 유발하지 않음을 알 수 있었다. 아울러, 각 처리구에서 사양한 해산물의 어육부에 오염된 Vibrio vulnificus의 균수를 측정한 결과, 250ppm 이상의 GFSE 처리구에서는 Vibrio vulnificus를 전혀 검출할 수 없었다. To prevent food-poisoning outbreaks by Vibrio vulnificus the antimicrobial efficacy of grapefruit seed extract (GFSE) was examined. Minimal inhibitory concentration (in vitro) for the microorganism was found to be 50~100 ppm. Transmission electron micrographs of Vibrio vulnificus showed the biocidal action of this natural antimicrobial agent would be related to specific respiratory effect coupled with the destruction of permeable function of microbial cell membrane. After Anguilla japonia GFSE-injected to the body was incubated in the seawater contaminated by Vibrio vulnficus the fish meats were taken up, mixed with control diet and used as a diet in the feeding experiment. In this experiment the effect of GFSE treated with fish muscle on body weight protein efficiency ratio, serum enzymes and serum blood component of broiler chicks was investigated. It is proved from this study that there is neither Vibriosis nor toxicity associated with GFSE itself and fish meats treated with it when it is injected to the fish body at a level of 250 ppm or less.

수산물에 대한 Grapefruit 종자추출물의 항균 및 항산화효과

조성환,서일원,최종덕,주인생 國立統營水産專門大學 附設 水産科學硏究所 1990 수산과학연구소보고 Vol.2 No.-

연속 추출장치를 이용하여 grapefruit 종자를 gl-ycerine으로 추출하고 농축, 조제한 grapefruit 종자추출물(GFSE)의 항균 및, 항산화성 효과를 검토하기 위하여 정어리, 고등어 및 새우와 같은 수산물을 재료로 하여 GFSE를 처리하지 않은 대조구와 GFSE용액 (100, 250, 500, 750, 1,000PPM)으로 처리한 시험구로 나누어 Salmonella tylhi를 접종하여 일정 온도에서 저장하면서 총균수, 과산화물가 및 texture의 변화를 측정하고 GFSE처리후 저장한 수산물을 대조구와 외관, 냄새를 중심으로 관능검사를 실시하였다. 정어리와 고등어의 경우, 최초 균수가 1.9*10^6, 1.8*10^6이었는데 500PPM 농도 첨가하여 5℃에서 50일 저장후 1.1*10^4과 9.0*10^3으로 감소되었고, 새우의 경우 균의 검출이 확인되지 않아 상당히 좋은 항균효과를 나타내었다. 그리고, 3종의 수산물 모두 GFSE처리구가 대조구에 비하여 유의적인 항산화효과가 있음을 나타내었으며, 500~750PPM 농도의 용액을 처리하여 30℃에서 30일간 저장한 수산시료 추출유의 과산화물가는 정어리는 최초 28~45meq/kgdptj 92~143meq/kg(대조구,490meq/kg)로, 고등어는 64~75meq/kg에서 98~137meq/kg(대조구, 406meq/kg)로, 새우는12~20meq/kg에서 21~32meq/kg(대조구, 72meq/kg)로 증가하여 뚜렷한 산패 억제효과를 볼 수 있었다. Texture도 저장기간이 경과함에 따라 감소하는 경향을 나타내었으나 3종 수산물 모두 GFSE용액 처리시험구에서 감소율이 크게 낮아졌다. GFSE를 처리하여 0℃, 0일간 보관한 시험구와 대조구에 관한 관능검사 결과, GFSE를 처리하지 않은 대조구는 열등한 기호도를 보여준 반면, GFSE용액을 500PPM 이상의 농도로 처리한 시험구 모두가 높은 기호도의 관능검사 결관를 나타내었다. The antimicrobial and antioxidant activities of grapefruit seed extract(GFSE), which was extracted with glycerine in the special schematic extraction apparatus, were investigated for handling and processing of fishery products. The effectivity of GFSE has been tried on sardine, mackerel and shrimp divided into six lots for each fishery product: control(no treatment) and five GFSE-treated samples. Samples were inoculated with Salmonella typhi, incubated for 24hrs at 30℃ in dextrose-tryptone broth medium and prepared for microbiological & chemical analysis and organoleptic assessment. The bacteriological analytical results with GFSE(250ppm) showed the reduction of 1.8×10^6→2.0×10^4, 1.9×10^6→1.8×10^4 and 1.6×10^6→2.7×10^3 in total bacterial count for sardine, mackerel and shrimp, respectively. The test results with GFSE(500ppm) showed a 100% reduction of bacterial mackerel treated with GFSE(500ppm) was reduced to 1.1×10^4 and 9.0×10^3 respectively. Antioxidant effect of treatment with GFSE at 500ppm level for three products was significant. LSD test results on organoleptic parameter for the samples treated with various showed a significant influence on the appearance, odor and texture in which at concentration 500ppm level give the excellent scours compared to each control.

공복 시 Caffeine 투여 후 일회성 운동이 흰쥐의 인슐린 및 골격근의 GLUT-4 발현에 미치는 영향

정일규,오명진,김영표,김종오,윤재석,서태범,윤진환 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

This study was done to investigate the effects of caffeine ingestion on the expression of GLUT-4 in rats' skeletal muscle(soleus m) and blood insulin level after 12 hr fasting condition and one hour treadmill running. Total 19 rats(Sprague-Dawley) who were 7week Did were used for experimental subjects and raised for 1week in the room controled the air temperature and relative humudity(22℃ and 50%). They were divided three groups ; 12hr fasting group(F group, n=5), fasting+exercise group(FE, n= 7) and fasting+caffeine+exercise group(FCE group, n=7). FG and FCE group performed treadmill running for one hour and the treadmill speed was 18m/min that was supposed to be moderate exercise to rats. Each Dr rats in FCE group was forced to he given the caffeine solution(5mg/kg, Sigma Chemical co.) through stainless steel tube directly to their stomachs one hour before treadmill exercise. Immediately after treadmill running, we anesthetized them with the mixed solution(Ketamin 80g/kg and Rompun 5ml/kg) to draw 3ml blood from heart four insulin analysis and then obtained the rats' soleus muscle for GLUT-4 expression. We found that FCE group showed significantly higher GLUT - expression then the other groups and FE group showed the lowest GLUT-4 expression. This rusults suggest that the endurance type of exercise in fasting condition inhibit the GLUT-4 expression in working muscle and the caffeine helped to facilitate the GLUT-4 expression during endurance type of exercise. The future studies needed to confirm the action of caffeine to increase the GLUT- 4 and the mechanism.