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Low-Powered pH-Stable Nano-electrokinetically Enhanced Lateral Flow Assay for COVID-19 Antigen Test
Kim Kang Hyeon,유용경,Lee Na Eun,Lee Junwoo,Kim Cheonjung,Lee Seungmin,Kim Jinhwan,Park Seong Jun,Lee Dongtak,이상원,Kim Hyungseok,허돈,Yoon Dae Sung,Lee Jeong Hoon 한국바이오칩학회 2023 BioChip Journal Vol.17 No.3
Lateral fl ow assay (LFA) is a popular diagnostic system used in point-of-care testing (POCT) due to its low cost and portability. However, LFA has limited sensitivity and detection limits, making it challenging to detect low virus titers. Preconcentration through nano-electrokinetic (NEK) techniques have been proposed as a promising solution to improve the sensitivity of LFA. Nevertheless, the acidic conditions used in NEK operations may reduce the specifi city and sensitivity of LFA immunoassays. To address these limitations, an integrated LFA kit, the NEK-enhanced LFA (PcNEK–LFA), has been introduced. This kit features a pH-controlled structure designed to facilitate sample preconcentration. Biomarkers and AuNPs are electrokinetically preconcentrated in the PcNEK–LFA platform to increase the concentration of the test line and Ag–Ab binding events, resulting in enhanced performance. The pH-controlled PcNEK–LFA platform was evaluated using salivary human chorionic gonadotropin beta (β-hCG) and COVID-19 Ag samples, achieving a preconcentrating factor of approximately 10 and a sensitivity enhancement of 55.42%, and a preconcentrating factor greater than 10, respectively. The pH-controlled PcNEK–LFA platform provides an eff ective solution to overcome the limitations of LFA for POCT. In addition, it improves its sensitivity and detection limit, signifi cantly enhancing the accuracy and reliability of POCT, particularly for COVID-19 screening tests. As a result, this platform may play a pivotal role in addressing current and future healthcare challenges, facilitating rapid diagnosis and treatment of infectious diseases.
효율적인 인터넷 범죄수사를 위한 범행호스트 탐지 및 범죄행위 입증기술
김형석(Hyungseok Kim),김은진(Eunjin Kim),김휘강(Huy Kang Kim) 한국정보보호학회 2012 정보보호학회논문지 Vol.22 No.4
인터넷범죄를 수사함에 있어 가장 중요한 점은 범행호스트의 범행을 입증하는 것이다. 그러나 범죄자들은 범행부인을 위해 범행호스트의 IP주소를 변경하거나 패킷의 출발지 IP주소를 조작한다. 또한 악의적인 어플리케이션을 이용하여 범행기록을 남기지 않는다. 본 논문은 인터넷범죄수사의 한계를 극복하기 위한 Network Forensic Evidence Generation and Verification Scheme을 제안한다. 이 기술은 인터넷범죄수사를 위해 패킷의 생성위치와 전송과정에 주소필드가 조작되지 않았음을 보장하는 증거를 생성하여, 범행기록이 부재하여도 패킷을 통해 입증한다. 그리고 증거생성에 의한 라우터의 성능저하를 최소화하기 위해 Timestamp SecretKey Distribution Scheme과 Flow-Based Selection Scheme을 추가 제안한다. 마지막으로 제안한 기술들을 활용하기 위한 시스템을 구현하고 패킷전송률을 실험한다. One of the most important point in the Internet crime investigation is tracing back and pointing out a criminal host. However, criminals can forge a crime record stored in the crime host, or can utilize malicious applications in order not to leave a crime record. In addition, criminals can change the source IP address of a crime host and deny their involvement. In this study, we suggests the Network Forensic Evidence Generation and Verification Scheme (NFEGVS) to rectify the current limitation of Network Forensic technologies. This scheme can prove who and when the crime has occurred. In addition, this prevents leaking of symmetric key for guaranteeing certification and integrity of Forensic Evidence by proposing the Timestamp Secret Key Distribution Scheme, and minimizes performance degradation of router when generating forensic evidence with the Flow-Based Selection Scheme. In this paper, we implement the proposed scheme and evaluate overall performance of the proposed system.
( Hyungseok-jo ),( Kyubeom-kim ),( Seokhuen-ahn ),( Namhun-her ),( Sanghyeok-seo ),( Youngjung-kim ),( Byungchan-min ) 한국감성과학회 2019 한국감성과학회 국제학술대회(ICES) Vol.2019 No.-
The purpose of this study was to observe the correlation between illuminance and the color temperature that is suitable for the learning environment through the EEG. According to the characteristics of the color temperature it was divided into 3000K, 4000K, 6500K. The illuminance was set at 400lx and 1000lx as the KS standards. UK test was performed in each lighting situation. During the experiment, EEG were measured to see the central nervous system response. The experiment included 12 male that experienced learning in the classroom and reading room. The EEG were sampled at 512Hz using a TAOS VITALBRAIN, and the detected raw data were filtered through a 60Hz noise filter. After filtering, frequency analysis was performed. And power spectrum analysis was performed for αwave and βwave. We analyzed differences in the EEG based on the different lighting by 2-way ANOVA and post-hoc tests. The results of the study showed that a significant difference exists in terms of the EEG based on the illuminance and color temperature(p<0.05). Regarding the alpha power, 3000K-1000lx was higher than other lighting.
Hyungseok Hong,전형탁,Honggyu Kim,Hyungchul Kim,Sanghyun Woo,Seokhoon Kim,Seokhwan Bang,Seungjun Lee,Sunyeol Jeon,Wooho Jeong 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.4
We investigated the physical and the electrical properties of HfO₂ films grown by using a remote-plasma atomic layer deposition technique on a pre-deposited Hf metal layer. The Hf metal pre-deposited film retarded effectively the growth of an interfacial layer while the HfO₂ film without a Hf metal layer showed a growth of an interfacial layer. The as-deposited HfO₂ layers on thick Hf metal layers are crystallized while those on thin Hf metal layers remain amorphous. The pre-deposited Hf metal layer decreased the equivalent oxide thickness while it increased the crystallization temperature. The flat-band voltage (VFB) shifted in the negative direction with increasing pre-deposited Hf metal thickness and the effective fixed oxide charge density corresponding to the △VFB of the HfO₂ films increased with increasing pre-deposited Hf metal thickness. After annealing at 800℃, the VFB for the HfO₂ films shifted toward the ideal VFB and the effective fixed oxide charge decreased. The Hf pre-deposition technique was found to be effective in reducing the EOT by suppressing interfacial layer growth while the electrical properties, such as the leakage current density and the effective fixed oxide charge density, were degraded. We investigated the physical and the electrical properties of HfO₂ films grown by using a remote-plasma atomic layer deposition technique on a pre-deposited Hf metal layer. The Hf metal pre-deposited film retarded effectively the growth of an interfacial layer while the HfO₂ film without a Hf metal layer showed a growth of an interfacial layer. The as-deposited HfO₂ layers on thick Hf metal layers are crystallized while those on thin Hf metal layers remain amorphous. The pre-deposited Hf metal layer decreased the equivalent oxide thickness while it increased the crystallization temperature. The flat-band voltage (VFB) shifted in the negative direction with increasing pre-deposited Hf metal thickness and the effective fixed oxide charge density corresponding to the △VFB of the HfO₂ films increased with increasing pre-deposited Hf metal thickness. After annealing at 800℃, the VFB for the HfO₂ films shifted toward the ideal VFB and the effective fixed oxide charge decreased. The Hf pre-deposition technique was found to be effective in reducing the EOT by suppressing interfacial layer growth while the electrical properties, such as the leakage current density and the effective fixed oxide charge density, were degraded.
Adaptive interactions in shared virtual environments for heterogeneous devices
Kim, Soo Jeong,Gil, Kyung Jun,Kim, HyungSeok,Lim, Sang Boem,Kim, Jee-in John Wiley Sons, Ltd. 2010 Computer Animation and Virtual Worlds (Print) Vol.21 No.5
<P>In this research, we propose a method to adapt the interfaces and interaction processes of heterogeneous devices. The proposed method models the interaction capability of the devices. The interaction is modeled by categorizing elementary actions and measuring its effect in semantic behaviors. With this model, an interaction process can be modified to a given device by changing sequence of elementary actions for each behavior. In the pilot test, we showed the possibility of interaction adaptation for different situations. With the proposed adaptation mechanism, interface and interaction can be modeled for the device independently and can be transferred over different interaction environments. Copyright © 2010 John Wiley & Sons, Ltd.</P> <B>Graphic Abstract</B> <P> <img src='wiley_img_2010/15464261-2010-21-5-CAV338-gra001.gif' alt='wiley_img_2010/15464261-2010-21-5-CAV338-gra001'> </P>
Kim Dae Joong,Hwang Kun,Kim Hun,Cha Jang Gyu,Jang Hyungseok,Park Ju-Yong,Kim Yeo Ju 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.5
Objective: To evaluate the signal intensity of the periosteum using ultrashort echo time pulse sequence with threedimensional cone trajectory (3D UTE) with or without fat suppression (FS) to distinguish from artifacts in porcine tibias. Materials and Methods: The periosteum and overlying soft tissue of three porcine lower legs were partially peeled away from the tibial cortex. Another porcine tibia was prepared as three segments: with an intact periosteum outer and inner layer, with an intact periosteum inner layer, and without periosteum. Axial T1 weighted sequence (T1 WI) and 3D UTE (FS) were performed. Another porcine tibia without periosteum was prepared and subjected to 3D UTE (FS) and T1 WI twice, with positional changes. Two radiologists analyzed images to reach a consensus. Results: The three periosteal tissues that were partially peeled away from the cortex showed a high signal in 3D UTE (FS) and low signal on T1 WI. 3D UTE (FS) showed a high signal around the cortical surface with an intact outer and inner periosteum, and subtle high signals, mainly around the upper cortical surfaces with the inner layer of the periosteum and without periosteum. T1 WI showed no signal around the cortical surfaces, regardless of the periosteum state. The porcine tibia without periosteum showed changes in the high signal area around the cortical surface as the position changed in 3D UTE (FS). No signal was detected around the cortical surface in T1 WI, regardless of the position change. Conclusion: The periosteum showed a high signal in 3D UTE and 3D UTE FS that overlapped with artifacts around the cortical bone.