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Influence of 120 kDa Pyruvate:Ferredoxin Oxidoreductase on Pathogenicity of Trichomonas vaginalis
Hyun-Ouk Song 대한기생충학열대의학회 2016 The Korean Journal of Parasitology Vol.54 No.1
Trichomonas vaginalis is a flagellate protozoan parasite and commonly infected the lower genital tract in women and men. Iron is a known nutrient for growth of various pathogens, and also reported to be involved in establishment of trichomoniasis. However, the exact mechanism was not clarified. In this study, the author investigated whether the 120 kDa protein of T. vaginalis may be involved in pathogenicity of trichomonads. Antibodies against 120 kDa protein of T. vaginalis, which was identified as pyruvate:ferredoxin oxidoreductase (PFOR) by peptide analysis of MALDI-TOF-MS, were prepared in rabbits. Pretreatment of T. vaginalis with anti-120 kDa Ab decreased the proliferation and adherence to vaginal epithelial cells (MS74) of T. vaginalis. Subcutaneous tissue abscess in anti-120 kDa Ab-treated T. vaginalis-injected mice was smaller in size than that of untreated T. vaginalis-infected mice. Collectively, the 120 kDa protein expressed by iron may be involved in proliferation, adhesion to host cells, and abscess formation, thereby may influence on the pathogenicity of T. vaginalis.
한국형 삼일열말라리아의 약제내성 관련 유전자 변이 검색
송현욱 ( Hyun Ouk Song ),박민규 ( Min Kyu Park ),현창림 ( Chang Lim Hyun ) 국군의무사령부 2012 대한군진의학학술지 Vol.43 No.1
목적 1993년 한국에서 재유행하기 시작한 말라리아(삼일열말라리아, Plasmodium vivax)는 이후 꾸준히 발생이 증가하여 2000년에는 4000명넘게 발생하였다가 이후 2004년까지 급격히 감소하였다. 군에서는 발생 초기부터 말라리아 문제에 관심을 가지고 모기 박멸, 예방약 복용(클로로퀸과 프리마퀸) 등 적극적으로 대처해 왔지만 지금까지 환자가 꾸준하게 발생하고 있는 상황이다. 한국군은 우리나라 말라리아 재유행의 최초 보고지이며, 우리나라 말라리아 위험지역 내 부대가 다수 분포하고 있는 지리적 특성, 민간과 달리 예방약을 꾸준하게 사용하고 있는 특수성을 가지고 있다. 이에 이 연구는 말라리아의 약제 내성에 관한 특성을 연구하고자 계획되었다. 대상 및 방법 2008년~2009년 사이 국군 양주병원, 벽제병원, 일동병원에 내원한 말라리아 감염 환자 중 연구에 동의한 36명의 환자를 대상으로 연구를 시행하였다. 환자 동의 정도에 따라 내원 당일 혹은 내원일부터 4일까지 혈액을 채혈하여 전혈을 냉동 보관하여 국군의학연구소로 이송하고 연구에 사용하였다. 결과 환자들의 설문지를 분석한 결과 부대 근처에 산(80%)이나 수풀(55%)이 많이 있었고, 환자의 대다수가 상병(60%) 및 병장(24%)으로 나타나 말라리아 모기가 발생할 수 있는 여건에서 일정기간 이상 근무한 경우에 환자 발생이 많은 것으로 나타났다. 이들 중 클로로퀸과 프리마퀸을 정확히 복용하였다고 답한 수는 각각 24%와 12%에 불과해 약제의 불충분한 복용은 말라리아 환자발생의 한 원인으로 사료된다. 혈액에서 분리한 원충의 유전자 검사 결과 Pvmdr-1 부위의 변이가 최고 19.3% 확인되었다. 결론 부정확한 예방약 복용실태, 향후 예상되는 약제 내성 발현 가능성, 진단키트의 발달 및 보급가능 여부 등을 종합적으로 판단할 때 앞으로 군 내 말라리아 정책은 일선부대 진단키트 보급을 통한 빠른 진단 및 치료의 방향으로 가는 것이 바람직하다고 생각된다. Plasmodium vivax (vivax malaria) re-emerged in the South Korea in 1993. Annual incidence of malaria was steadily increased, reaching over 4,000 in 2000. After then, incidence was decreased in 2004, and once again increased, annual cases were reported over 2,000. Malaria has mainly occurred among the peoples in the areas near the Demilitarized Zone, especially soldiers who served in a unit that was in the Northern Gyeonggi-do, strongly suggests that malaria occurrence has been influenced by the present situation of malaria occurrence in the Democratic People’ 's Republic of Korea. The korean army had an interest in malaria prevention in early days and actively coped with the problem. Despite of mosquito eradication, and chemoprophylaxis with chloroquine and primaquine, malaria patients were raised steadily even now. This study were planned to study characteristics of drug resistance of malaria and will provide basic information for prevention and treatment of malaria in the korean army. In analysis of questionnaire of the patients who agreed with this study, majority of rank of the patients was corporal (60%) and sergeant (2%). Of these patients, only 24% and 12% of people exactly took chloroquine and primaquine, respectively. Moreover, analysis of the pvmdr1 and pvcrt-o site of plasmodium parasite which isolated from blood of patients shows some genetic variation. These result suggest that insufficient malaria drug intake plays a role in persistent case of malaria.
Whole-exome sequencing identifies recurrent <i>AKT1</i> mutations in sclerosing hemangioma of lung
Jung, Seung-Hyun,Kim, Min Sung,Lee, Sung-Hak,Park, Hyun-Chun,Choi, Hyun Joo,Maeng, Leeso,Min, Ki Ouk,Kim, Jeana,Park, Tae In,Shin, Ok Ran,Kim, Tae-Jung,Xu, Haidong,Lee, Kyo Young,Kim, Tae-Min,Song, Sa National Academy of Sciences 2016 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.113 No.38
<P>Pulmonary sclerosing hemangioma (PSH) is a benign tumor with two cell populations (epithelial and stromal cells), for which genomic profiles remain unknown. We conducted exome sequencing of 44 PSHs and identified recurrent somatic mutations of AKT1 (43.2%) and beta-catenin (4.5%). We used a second subset of 24 PSHs to confirm the high frequency of AKT1 mutations (overall 31/68, 45.6%; p.E17K, 33.8%) and recurrent beta-catenin mutations (overall 3 of 68, 4.4%). Of the PSHs without AKT1 mutations, two exhibited AKT1 copy gain. AKT1 mutations existed in both epithelial and stromal cells. In two separate PSHs from one patient, we observed two different AKT1 mutations, indicating they were not disseminated but independent arising tumors. Because the AKT1 mutations were not found to co-occur with beta-catenin mutations (or any other known driver alterations) in any of the PSHs studied, we speculate that this may be the single-most common driver alteration to develop PSHs. Our study revealed genomic differences between PSHs and lung adenocarcinomas, including a high rate of AKT1 mutation in PSHs. These genomic features of PSH identified in the present study provide clues to understanding the biology of PSH and for differential genomic diagnosis of lung tumors.</P>
‘Youhan’, A new whole crop barley cultivar of hooded spike and spring early regenerative type
Tae-Il Park,Young-Jin Oh,Ouk-Kyu Han,Hyoung-Ho Park,Jong-Chul Park,Sang-Kyun Cho,Jae-Hwan Noh,Dae-Wook Kim,Tae-Hwa Song,Hyun Seok Chae,Jae-Hyun Jeung,Ki-Heung Hong,Jeong-Suk Bae,Jae-Young Huh,Jin Song 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
‘Youhan’ (Hordeum vulgare L.), a new whole crop barley cultivar, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA in 2012. ‘Youhan’ has the growth habit of III, light green and middle size leaf, hooded and lax-type spikes. The cultivar showed 107 cm of culm length, 641 spikes per m2. Heading date of ‘Youhan’ was May 1, one day later than that of check cultivar ‘Yuyeon’ in upland, and 2 days earlier than that of check in paddy field. Maturing time was similar to check cultivar ‘Yuyeon’ as June 4 in upland and May 31 in paddy field. ‘Youhan’ also showed better winter hardiness, the resistance to lodging and disease than those of check cultivar. The average forage dry matter yield in the regional yield trial was about 12.6 and 12.0 ton ha-1 in upland and paddy field, respectively, which were 6%, 5% higher than that of the check. It also showed 7.3% of crude protein, 26.8% of ADF(Acid Detergent Fiber), 47.8% of NDF(Neutral Detergent Fiber), and 67.7% of TDN(Total Digestible Nutrients), including higher grade of silage quality for whole crop barley. Fall sowing cropping of ‘Youhan’ is recommended only in areas where average daily minimum mean temperatures in January are higher than -8°C, and it should not be cultivated in mountain areas of Korea.
Diagnosis of HNF-1α mutations on a PNA zip-code microarray by single base extension
Song, Jae Yang,Park, Hyun Gyu,Jung, Sung-Ouk,Park, JaeChan Oxford University Press 2005 Nucleic acids research Vol.33 No.2
<P>In the present study, we exploited the superior features of peptide nucleic acids (PNAs) to develop an efficient PNA zip-code microarray for the detection of hepatocyte nuclear factor-1α (HNF-1α) mutations that cause type 3 maturity onset diabetes of the young (MODY). A multi-epoxy linker compound was synthesized and used to achieve an efficient covalent linking of amine-modified PNA to an aminated glass surface. PCR was performed to amplify the genomic regions containing the mutation sites. The PCR products were then employed as templates in a subsequent multiplex single base extension reaction using chimeric primers with 3′ complementarity to the specific mutation site and 5′ complementarity to the respective PNA zip-code sequence on the microarray. The primers were extended by a single base at each corresponding mutation site in the presence of biotin-labeled ddNTPs, and the products were hybridized to the PNA microarray. Compared to the corresponding DNA, the PNA zip-code sequence showed a much higher duplex specificity for the complementary DNA sequence. The PNA zip-code microarray was finally stained with streptavidin-<I>R</I>-phycoerythrin to generate a fluorescent signal. Using this strategy, we were able to correctly diagnose several mutation sites in exon 2 of HNF-1α with a wild-type and mutant samples including a MODY3 patient. This work represents one of the few successful applications of PNA in DNA chip technology.</P>
Dengue virus-Polymersome Hybrid nanovesicles for Real-Time Single Virus Tracking
( Hyun-ouk Kim ),( Woonsung Na ),( Minjoo Yeom ),( Seungjoo Haam ),( Daesub Song ) 대한인수공통전염병학회 2019 창립총회 및 학술대회 초록집 Vol.2019 No.1
Introduction: Dengue virus is one of the major infectious human pathogens worldwide. Currently, no antiviral drug has become available against the dengue virus induced diseases since little is known regarding how dengue virus interacts with host cells. Dengue virus-polymersome hybrid nanovesicles are powerful tools to explain the dynamics of host cell-virus interaction and tracking. Methods: Dengue virus-polymersome hybrid nanovesicles were prepared by dialysis method. We showed that DiD and BODIPY FL C5-Ceramide encapsulated dengue virus-polymersome hybrid nanovesicles triggered the formation of red and green fluorescence by using real-time fluorescence microscopy. The internalization to escape endosomal entrapment was determined by confocal laser scanning microscopy. Results: Fluorescence images of DiD and BODIPY FL C5-Ceramide encapsulated dengue virus-polymersome hybrid nanovesicles illustrated at different time points. For real-time virus tracking study, it is desirable to acquire images to obtain adequately high resolution to monitor the dynamics of host cell-virus interaction and tracking in living cells. Conclusion: This study exploited a virus-nanovesicles tracking technology to approach whether dengue virus interacts with autophagy. Therefore, we demonstrated that dengue virus-polymersome hybrid nanovesicles will be utilized for virus tracking studies to examine the mechanisms of viral infections.