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Lee, Hyojung,Lee, Eui-joon,Kim, Hyunseong,Lee, Gihyun,Um, Eun-Jin,Kim, Youngchul,Lee, Boo-Yong,Bae, Hyunsu S. Karger AG 2011 American journal of nephrology Vol.34 No.2
<P>Abstract</P><P><I>Background/Aims:</I> Bee venom (BV) therapy has been used to treat inflammatory diseases including rheumatoid arthritis in humans and in experimental animals. This study was conducted to examine the therapeutic effect of BV on established lupus nephritis in New Zealand Black/White (NZB/W) F1 female mice. <I>Methods:</I> Beginning at 18 weeks of age, mice were given a subcutaneous injection of either BV (3 mg/kg BW) or an equal volume of saline once a week until the end of the study. To examine the effect of BV on CD4+CD25+Foxp3+ regulatory T cells, splenocytes from NZB/W mice (23 weeks of age) were treated with BV (1 μg/ml) or PBS in the presence of anti-CD3ε (1 μg/ml) and anti-CD28 antibodies (4 μg/ml) for 48 h. <I>Results:</I> BV administration delayed the development of proteinuria to a significant extent, prevented renal inflammation, reduced tubular damage, and reduced immune deposits in the glomeruli. Interestingly, CD4+CD25+ regulatory T cells were significantly increased in vitro and in vivo after BV treatment. <I>Conclusion:</I> Collectively, the administration of BV that has immune modulating effects represents an applicable treatment of lupus nephritis in NZB/W F1 mice.</P><P>Copyright © 2011 S. Karger AG, Basel</P>
Cisplatin induced nephrotoxicity is inhibited by Taxilli Ramulus
Lee, Gihyun,Lee, Jun-Ho,Ham, Kyoung Keun,Lee, Hyojung,Kim, Hyunseong,Lee, Hyeonhoon,Hong, Moochang,Shin, Minkyu,Bae, Hyunsu 대한독성유전단백체학회 2012 Molecular & cellular toxicology Vol.10 No.2
Taxilli Ramulus has been shown have antioxidant and anti-inflammatory activities. Cisplatin is the most active cytotoxic anti-cancer drugs. However, the nephrotoxicity of cisplatin limits its use as a therapeutic. In the present study, we examined whether Taxilli Ramulus could protect against cisplatin induced acute renal failure in mice. Taxilli Ramulus were administrated orally once a day for 3 days (150 or 300 mg/kg body wt). After 3 days, all groups of mice received a single dose of cisplatin (25 mg/kg (body wt)) intraperitoneally. Taxilli Ramulus treatment ameliorated renal dysfunction 72 h after cisplatin injection. Taxilli Ramulus treated group had significantly reduced levels of proinflammatory cytokines when compared to the control group. These results show that Taxilli Ramulus is a potent herbal medicine that can reduce cisplatin nephrotoxicity. It also demonstrates that Taxilli Ramulus can prevent the renal toxic effects caused by cisplatin.
5-Lipoxygenase mediates RANKL-induced osteoclast formation via the cysteinyl leukotriene receptor 1.
Lee, Jung-Min,Park, Hyojung,Noh, A Long Sae Mi,Kang, Ju-Hee,Chen, Ling,Zheng, Ting,Lee, Juhyun,Ji, Sun-Young,Jang, Chang-Young,Shin, Chan Soo,Ha, Hyunil,Lee, Zang Hee,Park, Hea-Young,Lee, Dong-Seok,Yi American Association of Immunologists 2012 Journal of Immunology Vol. No.
<P>5-Lipoxygenase (5-LO) catalyzes the formation of two major groups of leukotrienes, leukotriene B4 and cysteinyl leukotrienes (CysLTs), and it has been implicated as a promising drug target to treat various inflammatory diseases. However, its role in osteoclastogenesis has not been investigated. In this study, we used mouse bone marrow-derived macrophages (BMMs) to show that 5-LO inhibitor suppresses RANKL-induced osteoclast formation. Inhibition of 5-LO was associated with impaired activation of multiple signaling events downstream of RANK, including ERK and p38 phosphorylation, and IκB degradation, followed by a decrease in NFATc1 expression. Ectopic overexpression of a constitutively active form of NFATc1 partly rescued the antiosteoclastogenic effect of 5-LO inhibitor. The knockdown of 5-LO in BMMs also resulted in a significant reduction in RANKL-induced osteoclast formation, accompanied by decreased expression of NFATc1. Similar effects were shown with CysLT receptor (CysLTR)1/2 antagonist and small RNA for CysLTR1 in BMMs, indicating the involvement of CysLT and CysLTR1 in 5-LO-mediated osteoclastogenesis. Finally, 5-LO inhibitor suppressed LPS-induced osteoclast formation and bone loss in the in vivo mouse experiments, suggesting a potential therapeutic strategy for treating diseases involving bone destruction. Taken together, the results of this study demonstrate that 5-LO is a key mediator of RANKL-induced osteoclast formation and possibly a novel therapeutic target for bone-resorption diseases.</P>
Minjeong Lee(Minjeong Lee),Hyojung Bae(Hyojung Bae),Hokyun Rho(Hokyun Rho),Vishal Burungale(Vishal Burungale ),Pratik Mane(Pratik Mane ),Chaewon Seong(Chaewon Seong),Jun-Seok Ha(Jun-Seok Ha) 한국마이크로전자및패키징학회 2020 마이크로전자 및 패키징학회지 Vol.27 No.4
The Cu/Cu2O/CuO photoelectrode has been successfully fabricated by Rapid Thermal Annealing technique. The structural characterization of fabricated photoelectrode was performed using X-Ray diffraction, while elemental composition of the prepared material has been checked with X-Ray Photoelectron Spectroscopy. The synthesis parameters are optimized on the basis of photoelectrochemical performance. The best photoelectrochemical performance has been observed for the Cu/Cu2O/CuO photoelectrode fabricated at 550 ℃ oxidation temperature and oxidation time of 50 seconds with highest photocurrent density of -3 mA/㎠ at -0.13 V vs. RHE.
Kyeongrin Bang,Hyojung Kwon,Minsup Lee,Jiae Lee,Sejung Hwang,Saeyoull Cho 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04
A full-length lysozyme cDNA from Gryllotalpa orientalis was cloned and sequenced. The deduced amino acid sequence of the lysozyme protein was 143 amino acids in length, with a calculated molecular mass of 15.84 kDa and an isoelectric point of 4.74. Sequence motifs, together with alignment and phylogenetic results, confirmed that G. orientalis lysozyme belongs to the C (chicken)-type lysozyme family of proteins. The protein sequence of lysozyme from G. orientalis showed high identity to that of Drosophila melanogaster (51.7%); however, in contrast to D. melanogaster lysozyme, G. orientalis lysozyme was immune inducible and expressed in a wide range of tissues. Expression of G. orientalis lysozyme mRNA was highest at 8 h post-infection and subsequently decreased with time after bacterial infection. We also expressed G. orientalis lysozyme protein in vitro using the pET expression system. Compared with the negative control, over-expressed G. orientalis lysozyme showed antimicrobial activity against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Bacillus subtilis by radial diffusion assay, with MIC values of 30.3 μM and 7.55 μM respectively. These results indicate that G. orientalis lysozyme may have stronger antimicrobial activity than other lysozymes against a broad range of microorganisms.
MinSoo Byun,Dahyun Yi,JunHo Lee,YoungMin Choe,BoKyung Sohn,JunYoung Lee,HyoJung Choi,Hyewon Baek,YuKyeong Kim,YunSang Lee,ChulHo Sohn,Inhee MookJung,Murim Choi,YuJin Lee,DongWoo Lee,SeungHo Ryu,ShinGy 대한신경정신의학회 2017 PSYCHIATRY INVESTIGATION Vol.14 No.6
Objective-The Korean Brain Aging Study for the Early Diagnosis and Prediction of Alzheimer’s disease (KBASE) aimed to recruit 650 individuals, aged from 20 to 90 years, to search for new biomarkers of Alzheimer’s disease (AD) and to investigate how multi-faceted lifetime experiences and bodily changes contribute to the brain changes or brain pathologies related to the AD process. Methods-All participants received comprehensive clinical and neuropsychological evaluations, multi-modal brain imaging, including magnetic resonance imaging, magnetic resonance angiography, [11C]Pittsburgh compound B-positron emission tomography (PET), and [18F]fluorodeoxyglucose-PET, blood and genetic marker analyses at baseline, and a subset of participants underwent actigraph monitoring and completed a sleep diary. Participants are to be followed annually with clinical and neuropsychological assessments, and biannually with the full KBASE assessment, including neuroimaging and laboratory tests. Results-As of March 2017, in total, 758 individuals had volunteered for this study. Among them, in total, 591 participants-291 cognitively normal (CN) old-aged individuals, 74 CN young- and middle-aged individuals, 139 individuals with mild cognitive impairment (MCI), and 87 individuals with AD dementia (ADD)-were enrolled at baseline, after excluding 162 individuals. A subset of participants (n=275) underwent actigraph monitoring. Conclusion-The KBASE cohort is a prospective, longitudinal cohort study that recruited participants with a wide age range and a wide distribution of cognitive status (CN, MCI, and ADD) and it has several strengths in its design and methodologies. Details of the recruitment, study methodology, and baseline sample characteristics are described in this paper.
Lee, Donghwa,Kim, Jongyoun,Kim, Honggi,Heo, Hyojung,Park, Kyutae,Lee, Youngu The Royal Society of Chemistry 2018 Nanoscale Vol.10 No.39
<P>An ultra-sensitive and transparent piezoresistive pressure sensor based on a sea-urchin shaped metal nanoparticle (SSNP)-polyurethane (PU) composite with microdome arrays is successfully fabricated for the first time. The piezoresistive pressure sensor with microdome arrays was prepared using a nanoimprinting process based on an intermediate polymer substrate (IPS) replica mold. It showed a superior sensitivity (71.37 kPa<SUP>−1</SUP>) and a high optical transmittance (77.7% at 550 nm) due to the effective quantum tunneling effect even at small concentrations of conductive SSNP filler (6 mg mL<SUP>−1</SUP>). The high-performance characteristics of the piezoresistive pressure sensor are attributed to the geometric effects of the microdome structure, especially the stress concentration at small contact spots and the deformation of the contact area. The piezoresistive pressure sensor with microdome arrays also exhibited a fast response/relaxation time (30 ms), ultra-low pressure detection (4 Pa), and excellent long-term stability under harsh conditions. In addition, the effectiveness of the piezoresistive pressure sensors in various sensing applications including sensing mapping, human arterial pulse monitoring, and the detection of muscle movement is also successfully demonstrated. It is anticipated that this novel transparent pressure sensor based on a SSNP-PU composite with microdome arrays will be a key component in the development of integrated transparent sensing applications.</P>
Lee, Seung Soo,Shin, Hyojung,Jo, Suah,Lee, Sun-Mi,Um, Youngsoon,Woo, Han Min Elsevier 2018 Enzyme and microbial technology Vol.114 No.-
<P><B>Abstract</B></P> <P>RNA-guided genome engineering technologies have been developed for the advanced metabolic engineering of microbial cells to enhance production of value-added chemicals in <I>Corynebacterium glutamicum</I> as an industrial host. In this study, the RNA-guided CRISPR interference (CRISPRi) was applied to rapidly identify of unknown genes for native esterase activity in <I>C. glutamicum</I>. Combining with the carboxyl esterase (MekB) protein sequence alignment, two target genes (the <I>cg0961</I> and <I>cg0754</I>) were selected for the CRISPRi application to investigate the possible native esterase in <I>C. glutamicum</I>. The recombinant strain with repressed expression of the <I>cg0961</I> gene exhibited almost no capability on degradation of methyl acetate as a substrate of carboxyl esterase. This result was also confirmed in the <I>cg0961</I> gene deletion mutant. Thus, we concluded that Cg0961 plays a major role of the native carboxyl esterase activity in <I>C. glutamicum</I>. In addition, CRISPRi demonstrated an application for gene identification and its function as another genetic tool for metabolic engineering in <I>C. glutamicum.</I> </P> <P><B>Highlights</B></P> <P> <UL> <LI> RNA-guided genome engineering technology was applied for the gene identification. </LI> <LI> Cg0961 plays a major role of the native esterase activity in <I>C. glutamicum</I>. </LI> <LI> CRISPR interference saved time for the laborious work to delete the target gene. </LI> <LI> CRISPR interference has potentials used for genome-wide gene identifications. </LI> </UL> </P>
[P136] Epidermolysis bullosa acquisita developed after chronic graft-versus-host disease
( Hyojung Kim ),( Ju Hee Han ),( Chul Hwan Bang ),( Hyun-min Seo ),( Ji Hyun Lee ),( Young Min Park ),( Jun Young Lee ) 대한피부과학회 2017 대한피부과학회 학술발표대회집 Vol.69 No.1
Epidermolysis bullosa acquisita (EBA) is a rare acquired blistering disorder caused by production of auto-antibody against type-VII collagen. About 2 cases of epidermolysis bullosa acquisita have been reported after allogenic bone marrow transplantation associated with chronic graft-versus-host disease (GVHD). Herein we describe a rare case of EBA after chronic GVHD.. A 41-year-old male presented with slightly pruritic and intermittently painful, diffuse, variable-sized, erythematous patches and large tense bullae with erosion on the whole body for 4 months. The patient was diagnosed as acute myeloid leukemia 3 years ago and had history of chronic oral, ocular and liver GVHD after allogenic peripheral blood stem cell transplantation. Skin biopsy was performed with direct immunofluorescent (IF) test on the lesional and perilesional skin respectively. Epidermis was not included in the specimen and mild perivascular lymphohistiocytic infiltration was observed in the upper dermis. Direct IF test showed IgG3 and C3 infiltration in the dermo-epidermal junction. For confirmative diagnosis, indirect IF test was performed and revealed serum antibody infiltration on the dermal side of the separated dermo-epidermal junction. Patient was finally diagnosed as EBA. Treatment was started with prednisolone 20mg-30mg daily, and mycophenolic acid 2g was added. During 3 months of treatment, lesion showed waxing and waning pattern.