Chemical Composition and Phytoestrogen Analysis of Iranian Black Pomegranate Juice Concentrate and Seeds

Choi,One-Kyun,Kim,Yong-Seong,Yu,Hye-Kyoung,Lee,Chan,Bang,Hyo-Pil,Yang,Deok-Chun,Kim,Young-Kee 한국자원식물학회 2003 Plant Resources Vol.6 No.1

In this study, as preliminary research for the development of natural estrogen supplement the chemical properties of Iranian black pomegranate juice concentrate and seeds were evaluated. Proximate compositions of pomegranate juice concentrate and seeds were as follows; crude lipid 0.4% and 8.2%, moisture 39.9% and 6.6%, crude protein 0.9% and 12.2%, ash 1.4% and 1.7%, and carbohydrate 42.0% and 84.5% respectively. Major amino acids are glutamic acid (1310.0ppm) and aspartic acid (896.2ppm) in juice concentrate, and glycine (611.1ppm) and arginin (401.6ppm) in seeds. Ascorbic acid has the highest concentration of 20.0mg/l00g in juice concentrate and 0.23mg/l00 in seeds. The compositions of unsaturated fatty acids such as linoleic acid and linolenic acid were higher than those of saturated fatty acids such as stearic palmitic acid. Major minerals were potassium, calcium and sodium, potassium was highest in both juice concentrate and seeds. Vitamins were composed of ascorbic acid (20.0mg/l00g), vitamin B$_1$ 수식 이미지(0.12mg/100g) and niacin (0.80mg/l00g) in juice concentrate, and only ascorbic acid(0.23mg/l00g) in seeds. Organic acids such as citric and L-malic acid were detected only in pomegranate juice concentrate. The contents of total polyphenols were 4.55g/L in juice concentrate and 3.5mg/l00g in seeds, respectively. Phytoestrogens detected in pomegranate juice concentrate and seeds were daidzein, quercetin, genistein and 17β-estradiol.

공동주택의 범죄발생 실태 및 범죄불안감에 관한 연구 : 부산진구 사례 중심으로 The Case Studies of Busan Jin-Gu

최열,김효숙,임하경 釜山大學校 都市問題硏究所 2003 都市硏究報 Vol.15 No.-

Security of residential environment is a basic desire as well as a necessary condition to make happy home. Specially a security from the crime is an important matter at the large scale and high density dwelling complex in the industrialized city. The concept of defensible space is the living environment for crime prevention and reduction in fear of crime, based on recognition that crime occurrences have influenced on the quality of residential environment. Recently Crime Prevention Through Environment Design (CPTED) is used a variety fields including housing, commerce, education and recreation facilities. So it became important factors to construct a good city. It means that possibility of a CPTED's application is very diverse. This study intended to understand the place where produces crime and affects the uneasiness and to propose the design's guideline of defensible space to residential site. Therefore this paper analyzed the differences of the case among the apartments, the mixed-use residential buildings, the multiplex houses.

항공우주용 복합재튜브의 충격압궤특성에 관한 연구

김영남,최효석,기경서,김기형,양인영 朝鮮大學校 機械技術硏究所 2000 機械技術硏究 Vol.3 No.1

본 연구의 목적은 준정적 및 충격실험에 대한 CFRP 박육부재의 에너지흡수특성에 관하여 고찰하고자 한다. 준정적실험은 신강좌굴실험기를, 충격실험은 수직식 충격압궤실험장치를 사용하였으며, 총 178개의 CFRP 박육부재에 대하여 준정적 및 충격압궤실험을 수행하였다. CFRP 원형관의 압궤실험시 그 응답특성은 매우 복잡하며, 압궤과정중 상이한 역학적 상호관계에 의존하게 된다. 본 연구에서는 다양한 적층조건을 갖는 박육부재의 압궤특성과 흡수에너지에 관하여 실험후 고찰하였다. 그 결과 CFRP 박육부재의 에너지 흡수 특성은 초기결함(Trigger)과 계면수 등에 관한 다양한 함수로 나타났으며, 일반적으로 CT-타입의 CFRP 박육부재의 에너지 흡수특성이 다른 박육부재에 비해 가장 높음을 알수 있었다. The purpose of this study is to investigate on the energy absorption characteristics of CFRP (Carbon Fiber Reinforced Plastics) tubes on quasi-static and impact tests. Quasi-static and impact compression tests were performed on 178 tubes. Static compression tests have been carried out using the static testing machine(Shin-gang buckling testing machine) and impact compression tests have been carried out using the vertical crushing testing machine. When such tubes are subjected to crushing loads, the response is complex and depends on the interaction between the different mechanisms that control the crushing process. The collapse characteristics and energy absorption of a variety of tubes have been examined. The results varied significantly as a function of trigger and interlaminar number. In general, the CT-type tubes absorbed more energy than the others.

Carrageenan 유도 관절염에 대한 灸律 藥鍼의 진통작용

구성태,신종근,최윤영,송정방,김재효,김경식,손인철 한국전통의학연구소 2006 한국전통의학지 Vol.15 No.1

Objectives: This study was produced to examine the effects of moxibustion that had been played important role to traditional oriental medical treatment on disease. Recently, it was reported that moxi-tar which is generated in the process of moxibustion as burning combustibles decreased nitric oxide(NO) and inducible NO synthase (iNOS) generation in cellular experiments. Methods: Carrageenan-induced arthritis rat model was used to test the effect of moxi-tar as a chronic pain model. Diluted moxi-tar was single injected in several acupoints or combined with electroacupuncture (1 ms, 2 Hz, and 2 mA) into contralateral ST36 acupoint for 30 min to assess the synergic effects. After the treatment, behavioral tests measuring stepping force were periodically conducted during the next 12 hours. Endogenous NO and iNOS, cyclooxygenase-2 (COX-2), and c-Fos protein expression in the spinal cord were examined on a rat model of carrageenan-induced arthritis. Results : After the induction of arthritis, rats subsequently showed a reduced stepping force of the affected limb for at least the next 4 days. The reduced stepping force of the limb was presumably due to a painful knee, since oral injection of indomethacin produced temporary improvement of weight bearing. Moxi-tar produced significant improvement of stepping force of the hindlimb affected by the arthritis lasting at least 9 hours. The magnitude of this improvement was equivalent to that obtained after an oral injection of 3 mg/kg of indomethacin and this improvement of stepping force was interpreted as an analgesic effect. Moxi-tar produced the improvement of stepping force of the affected hindlimb in a dose-dependent manner. Both NO production and iNOS, COX-2 protein expression increased by arthritis were suppressed by moxi-tar. Moxi-tar on combination with electroacupuncture (EA) produced more powerful and longer lasting improvement of stepping force of the hindlimb affected by the arthritis than either moxi-tar or EA did. Conclusion : The present study suggest that moxi-tar produces a potent analgesic effect on the chronic knee arthritis pain model in the rat and that moxi-tar-induced analgesia modulate endogenous NO through the suppression of iNOS/COX-2 protein expression.

정치 웹사이트를 대상으로 한 웹 공간 분석

박한우,한지은,최경호,김효동,Kate A. Mirandilla 한국정보사회진흥원 2006 정보화정책 Vol.13 No.1

본 연구의 목적은 필리핀의 정치 시스템 및 선거 과정에 있어 인터넷의 이용, 역할, 영향 등을 살펴봄으로써 사이버 공간에서 전개되는 정치 활동의 온라인 구조를 분석하는 것이다. 구체적으로 말하면, 본 논문은 지난 2004년의 필리핀 총 선거에서 인터넷이 이용되는 다양한 방식을 검토하여 선거에서의 인터넷의 역할을 필리핀의 정치 문화와 연관하여 탐구하였다. 방법론적으로 먼저, 지난 필리핀 선거의 웹 공간을 형성하는 여러 웹사이트들 가운데 92개가 표집되었다. 표본들을 대상으로 정보성 및 참여유도성 피쳐의 분포 현황을 운영자의 유형에 따라 조사하였다. 연구 결과에 따르면, 정당과 비정부기구에서 운영하는 웹사이트들의 역할이 두드러지는 경향이 나타났다. 정당과 비정부기구는 선거와 관련된 뉴스와 온라인 포럼의 운영 등 가장 많은 유형의 피쳐(feature)를 제공하였다. 후보자 웹사이트 또한 유권자들의 참여를 촉진하는 여러 컨텐츠와 서비스를 상당히 제공하고 있었다. 이러한 결과는 필리핀의 인터넷 정치 문화에 영향을 미친 정치 및 사회적 상황과 결부되어 논의되었다.

PINK1 positively regulates HDAC3 to suppress dopaminergic neuronal cell death

Choi, Hyo-Kyoung,Choi, Youngsok,Kang, HeeBum,Lim, Eun-jin,Park, Soo-Yeon,Lee, Hyun-Seob,Park, Ji-Min,Moon, Jisook,Kim, Yoon-Jung,Choi, Insup,Joe, Eun-Hye,Choi, Kyung-Chul,Yoon, Ho-Geun IRL Press 2015 Human molecular genetics Vol.24 No.4

<P>Deciphering the molecular basis of neuronal cell death is a central issue in the etiology of neurodegenerative diseases, such as Parkinson's and Alzheimer's. Dysregulation of p53 levels has been implicated in neuronal apoptosis. The role of histone deacetylase 3 (HDAC3) in suppressing p53-dependent apoptosis has been recently emphasized; however, the molecular basis of modulation of p53 function by HDAC3 remains unclear. Here we show that PTEN-induced putative kinase 1 (<I>PINK1</I>), which is linked to autosomal recessive early-onset familial Parkinson's disease, phosphorylates HDAC3 at Ser-424 to enhance its HDAC activity in a neural cell-specific manner. PINK1 prevents H<SUB>2</SUB>O<SUB>2</SUB>-induced C-terminal cleavage of HDAC3 via phosphorylation of HDAC3 at Ser-424, which is reversed by protein phosphatase 4c. PINK1-mediated phosphorylation of HDAC3 enhances its direct association with p53 and causes subsequent hypoacetylation of p53. Genetic deletion of PINK1 partly impaired the suppressive role of HDAC3 in regulating p53 acetylation and transcriptional activity. However, depletion of HDAC3 fully abolished the PINK1-mediated p53 inhibitory loop. Finally, ectopic expression of phosphomometic-HDAC3<SUP>S424E</SUP> substantially overcomes the defective action of PINK1 against oxidative stress in dopaminergic neuronal cells. Together, our results uncovered a mechanism by which PINK1–HDAC3 network mediates p53 inhibitory loop in response to oxidative stress-induced damage.</P>

발아조건과 배지종류 및 양액 농도에 따른 식물공장 재배 청경채와 상추의 묘 생육특성

최효길(Hyo-Gil Choi),권준국(Joon-Kook Kwon),박경섭(Kyoung-Sub Park),강윤임(Yun-Im Kang),조명환(Myeong-Whan Cho),노일래(Il-Rae Rho),강남준(Nam-Jun Kang) (사)한국생물환경조절학회 2011 생물환경조절학회지 Vol.20 No.4

본 연구는 식물공장에서 청경채와 상추의 발아조건과 육묘배지 및 육묘 시 양액농도가 묘의 생육에 미치는 영향을 구명하고자 수행되었다. 청경채와 상추의 발아율은 광의 유무와 온도처리(15, 20, 25℃) 간에 유의적 차이가 없었으나, 육묘배지 간에는 우레탄스펀지의 경우 파종 전ㆍ후 수분관리 유ㆍ무에 따라 큰 차이를 보였다. 즉, 우레탄스펀지 배지에서 파종전ㆍ후 수분관리를 하지 않았을 경우 암면에 비해 발아율이 현저히 낮았으나, 수분관리를 해주었을 때에는 암면보다 발아율이 높았으며, 묘의 생육도 암면과 차이가 없었다. 그리고 청경채의 생체중은 EC 1.0dSㆍm?¹에서 1.5dSㆍm?¹에서보다 1.8배 무거웠고, 0.5dSㆍm?¹에서보다는 6배 무거웠다. 상추의 생체중 또한 EC 1.0dSㆍm?¹이 1.5dSㆍm?¹와 0.5dSㆍm?¹에 비해 1.7배와 3.5배 각각 무거웠다. This experiment was conducted to study the effects of germination condition, nursery media and nutrient concentration in plant factory on the growth characteristics of pak-choi and lettuce seedlings. The results showed that temperature (15, 20, 25℃) and light conditions (lighting and no lighting) had no significant effect on germination rate of pak-choi and lettuce, whereas the interaction of growing media (rockwool and urethane sponge) and moisture management before and after sowing had significant effect on germination percentage. In case of no moisture management before and after sowing, germination percentage was much higher in rockwool medium than urethane sponge medium. But germination percentage of urethane sponge was higher than that of rockwool with applying moisture before and after sowing. At the nutrient concentration of EC 1.0 dSㆍm?¹ the fresh weight of pak-choi was heavier than in EC 1.5 and 0.5 dSㆍm?¹ by 1.8 and 6.0 times, respectively. At the nutrient concentration of EC 1.0 dSㆍm?¹ the fresh weight of lettuce was also heavier than in EC 1.5 and 0.5 dSㆍm?¹ by 1.7 and 3.5 times, respectively.

Electrical conductivity and raman studies of B₂O₃-Na₂O-NaI glass systems

Choi, Byoung-KooShin, Kyoung-HeeLee, Hyo-Kyoung 檀國大學校 基礎科學硏究所 1995 論文集 Vol.7 No.-

Reversible SUMOylation of TBL1-TBLR1 Regulates β-Catenin-Mediated Wnt Signaling

Choi, Hyo-Kyoung,Choi, Kyung-Chul,Yoo, Jung-Yoon,Song, Meiying,Ko, Suk ,Jin,Kim, Chul ,Hoon,Ahn, Jin-Hyun,Chun, Kyung-Hee,Yook, Jong ,In,Yoon, Ho-Geun Elsevier 2011 Molecular cell Vol.43 No.2

<P><B>Summary</B></P><P>Dysregulation of Wnt signaling has been implicated in tumorigenesis. The role of Transducin β-like proteins TBL1-TBLR1 in the promotion of Wnt/β-catenin-mediated oncogenesis has recently been emphasized; however, the molecular basis of activation of Wnt signaling by the corepressor TBL1-TBLR1 is incompletely understood. Here, we show that both TBL1 and TBLR1 are SUMOylated in a Wnt signaling-dependent manner, and that this modification is selectively reversed by SUMO-specific protease I (SENP1). SUMOylation dismissed TBL1-TBLR1 from the nuclear hormone receptor corepressor (NCoR) complex, increased recruitment of the TBL1-TBLR1-β-catenin complex to the promoter of Wnt target genes, and consequently led to activation of Wnt signaling. Conversely, SENP1 decreased formation of the TBL1-TBLR1-β-catenin complex, leading to inhibition of β-catenin-mediated transcription. Importantly, inhibition of SUMOylation significantly decreased the tumorigenicity of SW480 colon cancer cells. Thus, our data reveal a mechanism for activation of Wnt signaling via the SUMOylation-dependent disassembly of the corepressor complex.</P> <P><B>Highlights</B></P><P>► Both TBL1 and TBLR1 are SUMOylated in a Wnt signaling-dependent manner ► SUMOylation dismissed TBL1-TBLR1 from the NCoR/SMRT corepressor complexes ► SENP1-mediated deSUMOylation decreased formation of the TBL1-TBLR1-β-catenin complex ► DeSUMOylation of TBL1-TBLR1 suppressed the tumorigenicity of SW480 colon cancer cells</P>

Self-reported Food Intolerance in Korean Patients With Irritable Bowel Syndrome

Hyo Jeong Lee,Hyun Jin Kim,Eun Hee Kang,Kee Wook Jung,Seung-Jae Myung,Yang Won Min,Chang Hwan Choi,Han Seung Ryu,Jong Kyoung Choi,Joong Goo Kwon,Kyoung Sup Hong,Kyung Sik Park 대한소화기 기능성질환∙운동학회 2019 Journal of Neurogastroenterology and Motility (JNM Vol.25 No.2

Background/Aims Various foods trigger and/or worsen the symptoms of irritable bowel syndrome (IBS). However, Korean food-related gastrointestinal (GI) symptoms in IBS patients have not yet been investigated. This study aims to evaluate the prevalence of self-reported food intolerance in Korean IBS patients and determine the Korean food items and food groups perceived by patients to worsen their GI symptoms. Methods We recruited 393 study subjects, comprising 101 IBS patients, 167 symptomatic non-IBS subjects, and 125 control subjects. All participants completed a questionnaire to identify the most problematic foods and assess the occurrence of GI symptoms caused by 119 Korean food items. They also completed the validated Rome III questionnaire for IBS. Results The prevalence of self-reported food intolerance in Korean IBS patients was 79.2%, which was significantly higher than that in control subjects (44.8%, P < 0.001). The most problematic foods reported by IBS patients who experienced food intolerance were high-fat foods (25.0%), gluten foods (23.8%), spicy foods (15.0%), and dairy products (15.0%). A total of 63.4% of IBS patients reported GI symptoms related to the consumption of foods high in fermentable oligo-, di-, mono-saccharides, and polyols (FODMAP), while 48.5% of IBS patients reported symptoms associated with high-fat foods. Gas problems and loose stools were the most frequently reported symptoms. Conclusions A large proportion of Korean IBS patients complained of intolerance to certain food items, with high-fat and high-FODMAP foods being the main triggers. This study provides a basis for planning food intervention studies for Korean IBS patients. Background/Aims Various foods trigger and/or worsen the symptoms of irritable bowel syndrome (IBS). However, Korean food-related gastrointestinal (GI) symptoms in IBS patients have not yet been investigated. This study aims to evaluate the prevalence of self-reported food intolerance in Korean IBS patients and determine the Korean food items and food groups perceived by patients to worsen their GI symptoms. Methods We recruited 393 study subjects, comprising 101 IBS patients, 167 symptomatic non-IBS subjects, and 125 control subjects. All participants completed a questionnaire to identify the most problematic foods and assess the occurrence of GI symptoms caused by 119 Korean food items. They also completed the validated Rome III questionnaire for IBS. Results The prevalence of self-reported food intolerance in Korean IBS patients was 79.2%, which was significantly higher than that in control subjects (44.8%, P < 0.001). The most problematic foods reported by IBS patients who experienced food intolerance were high-fat foods (25.0%), gluten foods (23.8%), spicy foods (15.0%), and dairy products (15.0%). A total of 63.4% of IBS patients reported GI symptoms related to the consumption of foods high in fermentable oligo-, di-, mono-saccharides, and polyols (FODMAP), while 48.5% of IBS patients reported symptoms associated with high-fat foods. Gas problems and loose stools were the most frequently reported symptoms. Conclusions A large proportion of Korean IBS patients complained of intolerance to certain food items, with high-fat and high-FODMAP foods being the main triggers. This study provides a basis for planning food intervention studies for Korean IBS patients.