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Micro cell array on silicon substrate using graphene sheet
Son, Hyeong-Guk,Oh, Hong-Gi,Park, Young-Sang,Kim, Dae-Hoon,Lee, Da-Som,Park, Woo-Hwan,Kim, Hyung Jin,Cho, Seung-Min,Lim, Ki Moo,Song, Kwang Soup Elsevier 2017 Materials letters Vol.196 No.-
<P><B>Abstract</B></P> <P>To fabricate micro-patterns for bioengineering applications, we used graphene sheet, metal mask, and plasma treatment rather than the commonly used photolithography process. Two types of micro-patterns were fabricated (line, and circle) on SiO<SUB>2</SUB>/Si (100, p-typed) substrate. In the line and circle micro-patterns, graphene etched areas were 100 and 150μm, respectively, with fluorinated graphene spacing. The efficiencies of early cell adhesion, which is necessary for the growth and proliferation of cells, were 62, 17, and 65% on the pristine, fluorinated, and etched graphene surface, respectively, for 6h of cell culture. After seeding the neuron cells on the patterned substrate, neuron cells proliferated and differentiated along the graphene etched regions. The graphene sheet was used as a passivation layer for micro-array of the neuron cell on SiO<SUB>2</SUB>/Si.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Graphene sheet has been used in the miniaturization process. </LI> <LI> Graphene sheet was partially etched by plasma treatment in O<SUB>2</SUB> gas environment. </LI> <LI> Graphene sheet was fluorinated by plasma treatment in C<SUB>3</SUB>F<SUB>8</SUB> gas environment. </LI> <LI> Fluorinated graphene sheet artificially control the adhesion of cell. </LI> <LI> Fluorinated graphene sheet was used as a passivation layer for micro-cell array. </LI> </UL> </P>
Characteristic of Neuroblastoma Cells Grown on Graphene Sheet
Oh, Hong Gi,Son, Hyeong Guk,Kim, Dae Hoon,Lee, Da Som,Jhee, Kwang Hwan,Park, Hye Bin,Kim, Hyung Jin,Cho, Seung Min,Lim, Ki Moo,Song, Kwang Soup American Scientific Publishers 2016 Journal of Nanoscience and Nanotechnology Vol.16 No.11
<P>We investigate the effect of cell culture conditions, using pristine graphene sheets as growth substrate, on the human nerve cell line (SH-SY5Y). The cell viability and morphology were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), lactate dehydrogenase (LDH) and caspase-3/7 activity assays, as well as fluorescence microscopy of cells stained with Hochest 33342 and Calcein AM. Human nerve cells exhibited 84% viability on pristine graphene sheets compared with control (cell culture polystyrene) after 3 days culturing. However, pristine graphene hardly induced apoptosis and allowed for 92% viability on necrosis, compared to positive control. Fluorescence data showed that the presence of graphene did not influence cell morphology. These results suggest that graphene sheets can be applied to advance tissue culture techniques for facilitating tissue repair and regeneration.</P>