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Application of an IPM-based Spray Program to Protected Cultivation of Strawberry in Korea
Myeong Hyeon Nam,Hyeon Suk Kim,Won Keun Lee,Yeol Kyu Seong,Mark. L. Gleason,Jeong Young Song,Hong Gi Kim 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.5
An IPM-based spray program (ISP), based on ecological and agronomic information, is necessary for reducing pesticide sprays and producing strawberries safely. In this study, the applicability of ISP to safe production of strawberries was compared with a conventional spray program (Con-SP) during the 2005-2006 and 2006-2007 seasons in plastic houses in Nonsan, Korea. The ISP program consisted of fungicide, insecticide, and miticide treatments as well as release of natural enemies before and after harvest. The ISP treatment schedule was as follows: dipping transplants in azoxystrobin before transplanting (mid-September); spraying with abamectin and thiacloprid when black mulch was applied (early October); spraying with pyraclostrobin + acetamiprid and tetraconazole + abamectin before blooming in early and mid-November, respectively; transplanting banker plants colonized by Aphidius colemani in mid-February (2005-2006 season) or November (2006-2007 season); and releasing Phytoseiulus persimilis in mid-March. Number of pesticide sprays per season for ISP and Con-SP was five and nine, respectively. Anthracnose crown rot, caused by Colletotrichum gloeosporioides, did not occur in ISP in either season, but incidence was ≤ 3.3% in Con-SP during the 2005-2006 season. Powdery mildew (Sphaerotheca aphanis var. aphanis) did not occur during harvest in ISP, but occurred during early and late harvest stages in Con-SP in both years. Incidence of Botrytis fruit rot did not differ between ISP and Con-SP. Two-spotted spider mite (Tetranychus urticae) did not appear until March in ISP; subsequently, spider mite density remained at ≤-0.5 mite per leaf until the last harvest period in both years. The number of spider mites in ISP and Con-SP during the harvest did not differ. Populations of cotton aphids (Aphis gossypii) were also higher in ISP than Con-SP but decreased from March to April, accompanied by production of aphid mummies by A. colemani. The results showed that ISP was more effective than Con-SP, and required fewer pesticide sprays.
식용식물자원으로부터 활성물질의 탐색-6, 쑥갓(Chrysanthemum coronarium L.)으로부터 sterol의 분리
송명종 ( Song Myeong Jong ),홍윤희 ( Hong Yun Hui ),김동현 ( Kim Dong Hyeon ),김대근 ( Kim Dae Geun ),정인식 ( Jeong In Sig ),이윤형 ( Lee Yun Hyeong ),김성훈 ( Kim Seong Hun ),박미현 ( Park Mi Hyeon ),김인호 ( Kim In Ho ),권병옥 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4
The aerial parts of the Garland (Chrysanthemum coronarium L.) were extracted in MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. EtOAc fractions gave three steroid compounds through application of silica gel column chromatographies. The chemical structures of the steroids were determined by the interpretation of several spectral data, including NMR and MS as stigmast-5-en-3β-ol (1, β-sitosterol), stigmast-4-en-6β-ol-3-one (2), stigmast-4-en-6α-ol-3-one (3). Compounds 2 and 3 have been so far reported only in the aquatic plants, were isolated for the first time from the land plants.
뇌손상 환자에게 적용되는 관절운동이 두개강 내압에 미치는 영향
현미숙,한미라,홍성현,송동현,남명선,조선옥,송선옥,김영주,Hyeon, Mi-Suk,Han, Mi-Ra,Hong, Seong-Hyeon,Song, Dong-Hyeon,Nam, Myeong-Seon,Jo, Seon-Ok,Song, Seon-Ok,Kim, Yeong-Ju 대한간호협회 2000 대한간호 Vol.39 No.1
Purpose: The study was. relating to the increase of intracranial pressure of patients in ICU with acute and subacute brain damage. carried out to determine if the ICP could be influenced by passive ROM exercise which is provided early after the operation in order to prevent articular contracture and muscle atrophy from regular ROM exercise for poor exercising ability. Method : Among the patients in Neurosurgical Intensive Care Unit(ICU) who got operation on brain damage, 19 ones whose ICP could be measured through extraventricular drainage system or Camino ICP catherer were selected. Each patient's intracarinial pressure, systolic blood pressure, mean arterial pressure, heart rate and respiration rate were checked six times, before and 1. 2, 3. 4, 5 minutes after the ROM exercise. From this, the cerebral perfusion pressure was calculated and the maximum increase of ICP during the ROM exercise was measured. Details of the passive ROM exercise : As for upper limb, the exercise was in the order of flexion & extension of shoulder and arms, adduction & abduction, shoulder rotation, supination & pronation of elbow, and flexion & extension of wrist. For lower limb, the order was flexion &. extension of hip and knee. adduction & abduction of hip, flexion & extension of hip, and flexion & extension of ankle. Each exercise was tried 10 times and proceed to the next region. Results : As a result of consecutive exercises, six exercise for upper limb and 4 for lower limb, the pressure was slightly increased, as compared with resting one, by average 0.89mmHg(p= .0002) in the flexion & extension of shoulder and arm. O.64mmHg(p= .02) in the flexion & extension of hip and knee. As for the same condition of exercise times. that is. the data(before and 1. 2. 3, 4. 5 minutes after the exercise) of the first day only showed that every parameters like intracranial pressure, systolic blood pressure. heart rate. and mean arterial pressure had no change before and after the exercise. The last. the exercise was repeated 1. 2, and 3 days to measure the influence of repeated exercise. The result showed that there was little change in cerebral perfusion pressure. systolic blood pressure. heart rate. and resp. rate but faint change in intracrainal pressure. The intracranial pressure was affected slightly on POD(p=.0358. f=4.74) and exercise day(p=.0452, f=4.23); decreased by about 1.15 mmHg with every 1 day after the brain surgery and increased by about 1.32 mmHg with every 1 exercise day. Discussion : The results showed that the stimulus of ROM exercise for a given time didn't affect on intracranial pressure and cerebral perfusion pressure. Also, the repeated exercise for 3 days decreased intracranial pressure down to almost stable pressure with the postoperation days. This tells that the influence of ROM exercise reduce as patient's condition after brain damage get away from the acute period. That is, the increase of intracranial pressure due to ROM exercise is slight as long as patient's condition after brain damage become more serious no longer.
Development of a type II diabetic mellitus animal model using Micropig®
Myeong-Seop Lee,Hee-Jun Yang,Chester D. Solis,Ki-Duk Song,Soo-Hyeon Kim,Woon-Kyu Lee 한국실험동물학회 2012 Laboratory Animal Research Vol.28 No.3
Diabetes, which has shown an explosive increase in terms of its incidence, is regarded as a serious disease that must be overcome for the sake of human life. Among animal models used for testing of drug efficacy, the mini-pig model has shown a rapid upload due to its many similarities with human, particularly concerning the pharmacokinetics of compounds after subcutaneous administration, the structure and function of the gastrointestinal tract, the morphology of the pancreas, and overall metabolic status. Based on these various advantages, we sought to develop an animal model of type II diabetic mellitus using the Micro-pig, which differs from other miniature pigs. We used six male Micro-pigs for induction of a moderate insulin deficient model with nicotinamide (NIA)/streptozotocin (STZ) treatment and three animals for control. For evaluation of incidence of type II diabetes, we measured blood glucose level, and performed oral glucose tolerance test and immunohistochemistry on pancreatic tissue using insulin antibody. Compared to control animals, all animals treated with NIA/STZ showed high levels of glucose and low levels of insulin. In addition, we observed the partially destroyed beta cell population from tissue of the pancreas in treated animals. Based on these results, we report that the Micro-pig model developed in this study can be used for testing of the efficacy of therapeutic agents for treatment of Type 2 diabetic mellitus.
Song, Jeong Young,Seo, Mun Won,Kim, Sun Ick,Nam, Myeong Hyeon,Lim, Hyoun Sub,Kim, Hong Gi The Korean Society of Mycology 2014 Mycobiology Vol.42 No.2
We analyzed the genetic diversity of Cylindrocarpon destructans isolates obtained from Korean ginseng (i.e., Panax ginseng) roots by performing virulence tests and nuclear ribosomal gene internal transcribed spacer (ITS) and mitochondrial small subunit (mt SSU) rDNA sequence analysis. The phylogenetic relationship analysis performed using ITS DNA sequences and isolates from other hosts helped confirm that all the Korean C. destructans isolates belonged to Nectria/Neonectria radicicola complex. The results of in vivo and ex vivo virulence tests showed that the C. destructans isolates could be divided into two groups according to their distinctive difference in virulence and the genetic diversity. The highly virulent Korean isolates in pathogenicity group II (PG II), together with foreign isolates from P. ginseng and P. quinquefolius, formed a single group. The weakly virulent isolates in pathogenicity group I, together with the foreign isolates from other host plants, formed another group and exhibited a greater genetic diversity than the isolates of PG II, as confirmed by the mt SSU rDNA sequence analysis. In addition, as the weakly virulent Korean isolates were genetically very similar to the foreign isolates from other hosts, they were likely to originate from hosts other than the ginseng plants.
( Myeong Su Chu ),( Hyeon Jeong Yun ),( Myeong Jun Song ),( Seung Won Jung ),( Young Seok Kim ),( Si Hyun Bae ),( Jong Young Choi ),( Sang Wook Choi ),( Seung Kew Yoon ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1
Aims: Biopsy is still the gold standard for the diagnosis of nonalcoholic steatohepatitis (NASH) but the definition may vary in evaluation of biopsies for clinical trials. Recently, a scoring system (steatosis, activity, fibrosis[SAF]) allowing the use of an algorithm (fatty liver inhibition of progression [FLIP]) reported for the classification of liver injury in morbid obesity. We evaluated the application of SAF score and FLIP algorithm in Korean patients with nonalcoholic fatty liver disease (NAFLD). Methods: We analyzed the 126 patients with biopsy-proven NAFLD in 5 centers in Korea from Aug, 2008 to April, 2016. Results: In total study populations, 56 patients (44.4 %) were diagnosed to NASH, 70 (55.6%) as NAFLD including 60 patients of a gray zone (NAS 3-4) according to NAS scoring system. Based FLIP algorithms, 72 patients (57.1 %) were categorized as NASH, 54 (42.9 %) as NAFLD. 21 patients (35%) in gray zone (NAS 3-4) and 5 patients (NAS ≥5) were categorized as NASH and steatosis, respectively. The activity score (ballooning+ lobular inflammation) enabled discriminating NASH. All patients with NASH had A ≥3, whereas 3 patients (5.2%) with A2 had NASH. Especially, the presence of ballooning in activity score was the significant factor to discriminate NASH and steatosis. This activity score was also closely correlated with both alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and Fragmented cytokeratin-18, respectively (P=0.000, 0.023, 0.006 respectively). Fibrosis grade by SAF score was significantly correlated with NAFLD fibrosis score and fibroscan (P=0.026, 0.000, respectively). Conclusions: The FLIP algorithm and SAF score system in the NAFLD may provide the favorable tools in Korean patients. Among the SAF score, the ballooning of hepatocyte may be an important factor to discriminate NASH and NAFLD, although it is one of the major limitations of liver biopsy.