Recombinant canstatin inhibits angiopoietin‐1‐induced angiogenesis and lymphangiogenesis

Hwang‐,Bo, Jeon,Yoo, Ki Hyun,Park, Jong‐,Hwa,Jeong, Han‐,Sin,Chung, In Sik Wiley Subscription Services, Inc., A Wiley Company 2012 International journal of cancer: Journal internati Vol.131 No.2

<P><B>Abstract</B></P><P>We describe the effect of recombinant canstatin, the NC1 domain of the α2 chain of Type IV collagen, on suppression of angiogenesis and lymphangiogenesis both <I>in vitro</I> and <I>in vivo</I>. Recombinant canstatin produced from stably transformed <I>Drosophila</I> S2 cells reduced the expression of angiopoietin‐1 in hypoxia mimetic agent, CoCl<SUB>2</SUB>‐treated CT‐26 cells. Recombinant canstatin inhibited proliferation, tube formation and migration of human angiopoietin‐1 (rhAngpt‐1)‐treated human umbilical vein endothelial cells (HUVEC) and lymphatic endothelial cells (LEC). Recombinant canstatin suppressed the expression of Tie‐2 and vascular endothelial growth factor‐3 (VEGFR‐3) transcripts in rhAngpt‐1‐treated HUVEC and LEC, respectively. The inhibitory effect of recombinant canstatin on tumor growth was also investigated using a heterotopic CT‐26 colon carcinoma animal (BALB/c mice) model. Recombinant canstatin reduced the final volume and weight of tumors, and blood and lymphatic vessel densities of tumors, which were evaluated by CD‐31 and LYVE‐1 immunostaining. Immunohistochemical analysis showed that recombinant canstatin dramatically reduced the expression of angiopoietin‐1 in CT‐26 colon carcinoma‐induced tumor, but not the expression of VEGF‐C. Tie‐2 and VEGFR‐3 expressions were also reduced in recombinant canstatin‐treated tumors. These results indicate that recombinant canstatin has anti‐tumoral activities against CT‐26 colon carcinoma cells. Recombinant canstatin reduces the expression of angiopoietin‐1 in hypoxia‐induced CT‐26 cells and inhibits the angiogenic and lymphangiogenic signaling induced by angiopoietin‐1. Recombinant canstatin probably inhibits angiogenesis and lymphangiogenesis <I>via</I> suppression of the integrin‐dependent FAK signaling induced by angiopoietin‐1/Tie‐2 and/or VEGFR‐3.</P>

기능적 언어중재 프로그램이 언어장애 아동의 언어능력에 미치는 영향

황보명 대불대학교 2003 論文集 Vol.9 No.1

This study was aimed atclarifying the effects of a Functional Language Intervention in children with language disoders. Five subjects were selected and two speech-language pathologists engaged in the functional language intervention. In the functional language intervention, activities consisted of group time, palying riddles time, following directions time, drawing time, cooking time, word-chain game time, role-playing time, searching for objects based on directions, and recreation game time. To compare pre-treatment with post-treatment in the children with language disorders, repeated-measures ANOVA was used. The results were as follows; First, the Functional Language Intervention was effective in improving the number of communicative intentions of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of communicative intentions. Second, the Functional Language Intervention was effective in improving the number of semantic relations of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of semantic relations. Third, the Functional Language Intervention was effective in improving the overall language abilities of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the overall language abilities.

공주군내 일부국민학교 아동의 학교 및 가정사고에 대한 조사

황보연 공주교육대학교 초등연구원 1977 公州敎大論叢 Vol.13 No.2

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부산지역 산성강우의 장기적인 화학적 성상의 변화

황성욱,전보경,김찬훈,서정민,최금찬 동아대학교 환경문제연구소 2001 硏究報告 Vol.24 No.1

It is important to know the impacts of acid rain because of soil acidification, reduced biological productivity. Many researchers were interested in regional acid problems, and former research were focued in short-term trends of acid rain. It is not easy to evaluate acid rain problems because of many parameters relating to meteorological factors, which are wind, temperature, humidity and other factors. Rainfall sampling for this study was done from September 1933 to December 2000 in Dong-A University, Busan. All Samples were collected in separately to early fraction and succeeding fraction. Also, the samples were measured for pH, and EC, cations and anions were analysed by ion Chromatography.

대학생의 상해에 관한 조사연구

황보연 / 김수균 공주교육대학교 초등연구원 1978 公州敎大論叢 Vol.14 No.2

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The Anglo-Saxon Chronicle의 어순연구 : Parker 및 Peterborough본을 중심으로

황보근 대구대학교 인문과학연구소 1988 人文科學硏究 Vol.6 No.-

The main purpose of this paper is to describe synchronic word-order types of the Parker and the Peterborough manuscripts of the Anglo-Saxon Chronicle and to explain diachronic word-order changes. The manuscripts were divided, according not to the syntactic characteristics of the sentences analyzed but to the compilers, into four sections: (Ⅰ) 878∼891, (Ⅱ) 925∼955, 971∼1001, (Ⅲ)1122∼1131 and (Ⅳ) 1132∼1154. The sentences in each entry year were analyzed and counted on the basis of three clause types: main, subordinate and relative. The number of clause types in the sections of the ASC is as follows: Ⅰ Ⅱ Ⅲ Ⅳ Main Clauses 113 65 329 249 Subordinate Clauses 13 7 109 81 Relative Clauses 28 7 66 28 Description of the word-order types led to the following conclusions: 1) The dominant word-order types in the main clause are SVX, SXV and X°VS. The frequency of SVX constantly increases from Section Ⅱ through Section Ⅳ. This type tends to have the topical or the semi-topical subject and to be used as the new information sentence conjoined with the preceding X°VS type. The SXV has the topical nominal or pronominal subject, and the pre-verbal X is mostly pronominal. The high frequency of X°VS in Section Ⅰ is due to its use as "the new event order" though it has the topical subject. The decrease in both SXV and X°VS is relatively constant. 2) In the subordinate and relative clauses SXV, SVX are the two dominant orders in Section Ⅰ. But from Section Ⅱ though Section Ⅳ the decrease in SXV is constant, and is coincident with the decrease in the main clause. The reason is to be explained by the hyphothesis that the order of elements in dependent clauses will be changed by analogy to the order of elements in independent clauses.

소도시 학생의 의료비지출에 관한 조사연구 -공주군내 초등학교 아동을 중심으로-

황보연, 이유복 공주교육대학교 초등연구원 1974 公州敎大論叢 Vol.11 No.-

In order to study Medical care expenditures spent by elementary school students of Gong Ju-gun on sickness, surveys conducted on the elected 4 schools consisting of 625 students during the 9 month period from March 1 to December 20, 1973. The following results have been obtained. 1. The average number of pers persons ons per household were 6.24. 2. OF all the households of students surveyed, 47.2 per cent were for agricultual] occupations and 20.5 per cent were clerical workers. 3. Illiterates constituted 13.4 per cent, primary school graduates 36.6 per cent, high? school graduates 18.5 per cent, and colleg graduates 13.9 per cent. 4.The average monthly income per household was 34.000 won. (47,000 won out of A area and 21;000 won out of B area) 5. The monthly average Number of absences from school due to sickness were 423.9’ case. April. June. October were the Highest months of the sickness ratio which total's 3,815 case, and which means 1,008 times of absence of each sick student. 6. The sickness of most students who needed medical care was cold, tiredness, and sto-- mach ace, etc. 7. The average monthly amount of medical care spent were 1,930 won for a households expendkures and 302 won per person. (373. 4 won out of A area and 236.8 won out of B area)

"요구하기" 사회적 언어중재가 언어장애 유아의 사회적 언어 자발 표현에 미치는 효과

황보명,강수균 한국언어치료학회 1997 言語治療硏究 Vol.6 No.1

The purpose of this study is to investigate how Social Language Intervention affect the spontaneous social language of language disordered children in the language tharapy room. For this purpose, three preschool children with language disorder were selected in the integrated preschool and nonintegrated preschool settings. Social Language Intervention of this study was limited to 'Request' among the categories of the pragmatic funtion of the social language. Using multiple baseline design accross subjects, the frequency of the spontaneou s 'Reguest' in response to the examiner's mand was measured. The frequency of the spontaneous 'Request' during free-play in the integrated and nonintegrated preschool settings was analyzed to see generalization of newly acquired social language. The results obtained from the present study are as follows: Firstly, the frequency of the spontaneous social language was increased through Social Language Intervention for children both in integrated and nonintegrated set tings. However, the generalization effect occurred significantly more often in children treated in the integrated setting. Secondly, Social Language Intervention increased the frequency of the spontaneous speech in 'Suggestion' and 'Action Request' among the subcategories of ' Request.' The other subcategories(i.e., 'Question' and 'Approval Request') stayed almost the same. In addition, children treated in the integrated setting showed better improvement in terms of frequency of spontaneous 'Suggestion' and 'Action Request.' Thirdly, Social Language Intervention increased the frequency of the spontaneou s 'Assertion' social language in children treated in the integrated setting than nonintegrated setting. Finally, Social Language Intervention increased the frequency of the spontaneou s 'Response' in children treated in the integrated setting than nonintegrated setting.

副詞的 代名詞 硏究

皇甫淳 慶北大學校 語學硏究所 1980 語文硏究 Vol.5 No.-

형제 참여 언어중재가 언어장애 아동의 언어능력에 미치는 효과

황보명 한국음성과학회 2003 음성과학 Vol.10 No.3

This study was aimed at clarifying the effects of sibling-involved language intervention in children with language disorders. Ten subjects were selected an assigned into two groups (language intervention group with sibling-involvement, a language intervention without sibling-involvement). Group 1 consisted of 5 children with language disorders. Group 2 consisted of 5 children with language disorders without sibling-involvement. To investigate the language and communication ability, Picture Vocabulary est. and the subjects's spontaneous speech sample were analyzed. To compare pre-treatment with post-treatment between two the groups, repeated-measures ANOVA and t-test were used. The results were as follows; First, the language intervention with sibling-involvement was not effective compared to without sibling-involvement in improving the picture vocabulary ability of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the picture vocabulary ability of the two groups. However, there was no significant difference in the post-trearment between the two groups. Second, the language intervention with sibling involvement was more effective compared to without sibling-involvement in improving the semantic aspects of language of children with language disorders. There was a significant difference between per-treatment and post-treatment in the number of semantic elations between the two groups in that group 1 showed a higher improvement than group 2. Third, the language intervention with sibling involvement was more effective in improving pragmatic aspects of language of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of communicative intentions of the two groups. And there was a significant difference in the post-treatment between the groups in that group 1 showed a higher improvement than group 2.