Recombinant canstatin inhibits angiopoietin‐1‐induced angiogenesis and lymphangiogenesis

Hwang‐,Bo, Jeon,Yoo, Ki Hyun,Park, Jong‐,Hwa,Jeong, Han‐,Sin,Chung, In Sik Wiley Subscription Services, Inc., A Wiley Company 2012 International journal of cancer: Journal internati Vol.131 No.2

<P><B>Abstract</B></P><P>We describe the effect of recombinant canstatin, the NC1 domain of the α2 chain of Type IV collagen, on suppression of angiogenesis and lymphangiogenesis both <I>in vitro</I> and <I>in vivo</I>. Recombinant canstatin produced from stably transformed <I>Drosophila</I> S2 cells reduced the expression of angiopoietin‐1 in hypoxia mimetic agent, CoCl<SUB>2</SUB>‐treated CT‐26 cells. Recombinant canstatin inhibited proliferation, tube formation and migration of human angiopoietin‐1 (rhAngpt‐1)‐treated human umbilical vein endothelial cells (HUVEC) and lymphatic endothelial cells (LEC). Recombinant canstatin suppressed the expression of Tie‐2 and vascular endothelial growth factor‐3 (VEGFR‐3) transcripts in rhAngpt‐1‐treated HUVEC and LEC, respectively. The inhibitory effect of recombinant canstatin on tumor growth was also investigated using a heterotopic CT‐26 colon carcinoma animal (BALB/c mice) model. Recombinant canstatin reduced the final volume and weight of tumors, and blood and lymphatic vessel densities of tumors, which were evaluated by CD‐31 and LYVE‐1 immunostaining. Immunohistochemical analysis showed that recombinant canstatin dramatically reduced the expression of angiopoietin‐1 in CT‐26 colon carcinoma‐induced tumor, but not the expression of VEGF‐C. Tie‐2 and VEGFR‐3 expressions were also reduced in recombinant canstatin‐treated tumors. These results indicate that recombinant canstatin has anti‐tumoral activities against CT‐26 colon carcinoma cells. Recombinant canstatin reduces the expression of angiopoietin‐1 in hypoxia‐induced CT‐26 cells and inhibits the angiogenic and lymphangiogenic signaling induced by angiopoietin‐1. Recombinant canstatin probably inhibits angiogenesis and lymphangiogenesis <I>via</I> suppression of the integrin‐dependent FAK signaling induced by angiopoietin‐1/Tie‐2 and/or VEGFR‐3.</P>

펄스정전응력법을 이용한 고분자 재료에서 공간전하분표 측정에 대한 이론적 고찰

황보승 호남대학교 산업기술연구소 1998 산업기술연구논문집 Vol.6 No.-

본 논문에서는 최근 국 ·내외적으로 활발하게 연구되고 있는 고체 고분자 재료 내의 공간전하 측정법과 관련하여, 공간전하의 비파괴 측정법 중의 하나인 펄스정전응력법(PEA 법)에 대한 이론적 고찰을 수행하였다. 또한, 두꺼운 압전소자를 이용해서 PEA 법의 공간분해능을 향상시킬 수 있는 새로운 방법을 제안하였다. In this paper, we have evaluated theoretically the PEA method which is one of the most popular methods for the nondestructive measurement of space charge distribution in polymers. On the basis of this theoretical evaluation, we also suggest a new measuring method that uses a thick piexo-material for improving the space resolution of PEA method.

The Anglo-Saxon Chronicle의 어순연구 : Parker 및 Peterborough본을 중심으로

황보근 대구대학교 인문과학연구소 1988 人文科學硏究 Vol.6 No.-

The main purpose of this paper is to describe synchronic word-order types of the Parker and the Peterborough manuscripts of the Anglo-Saxon Chronicle and to explain diachronic word-order changes. The manuscripts were divided, according not to the syntactic characteristics of the sentences analyzed but to the compilers, into four sections: (Ⅰ) 878∼891, (Ⅱ) 925∼955, 971∼1001, (Ⅲ)1122∼1131 and (Ⅳ) 1132∼1154. The sentences in each entry year were analyzed and counted on the basis of three clause types: main, subordinate and relative. The number of clause types in the sections of the ASC is as follows: Ⅰ Ⅱ Ⅲ Ⅳ Main Clauses 113 65 329 249 Subordinate Clauses 13 7 109 81 Relative Clauses 28 7 66 28 Description of the word-order types led to the following conclusions: 1) The dominant word-order types in the main clause are SVX, SXV and X°VS. The frequency of SVX constantly increases from Section Ⅱ through Section Ⅳ. This type tends to have the topical or the semi-topical subject and to be used as the new information sentence conjoined with the preceding X°VS type. The SXV has the topical nominal or pronominal subject, and the pre-verbal X is mostly pronominal. The high frequency of X°VS in Section Ⅰ is due to its use as "the new event order" though it has the topical subject. The decrease in both SXV and X°VS is relatively constant. 2) In the subordinate and relative clauses SXV, SVX are the two dominant orders in Section Ⅰ. But from Section Ⅱ though Section Ⅳ the decrease in SXV is constant, and is coincident with the decrease in the main clause. The reason is to be explained by the hyphothesis that the order of elements in dependent clauses will be changed by analogy to the order of elements in independent clauses.

순천향의과대학 졸업생의 학부성적과 의사국가고시 성적의 관련성

황보영 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Objectives: This study analysed the correlation between grade point average (GPA) of medical school and the score of Korean Medical Licensing examination(KMLE). Methode: This study based on the result of the KMLE applicants who graduated from a college of medicine in 2003 and 2004. We also gathered data of these applicants from the college of medicine : gender, age, type of entrance, period of attendance, GPA of basic medicine, clinical medicine, clinical clerkships and final test scores. We analyed whether these variables affected the results of KMLE. Results: 184 applicants (90.6%) passed KMLE among 203. In the logistic regression test, GPAs of clinical medicine and final test scores were significant variables on the results of KMLE, after adjustment of covariates. Cut-off points of high risk group for failing KMLE were below 76.0(sensitivity 90; specificity 80; positive predictive value 32), 78.0(sensitivity 79; specificity 90; positive predictive value 46), 77.2(sensitivity 90; specificity 88; positive predictive value 43), 72.8(sensitivity 90; specificity 88; positive predictive value 44) in the GPAs of basic medicine, clinical medicine, clinical clerkships and final test scores, respectively. Conclusions: There were signficant associaton between the GPAs of clinical medicine and final test scores and the results of KMLE. High-risk groups of failing KMLE were the students whose GPA of baisc medicine were below 78.0, GPA of clinical clerkships were below 77.2 and final test score were below 72.8.

기능적 언어중재 프로그램이 언어장애 아동의 언어능력에 미치는 영향

황보명 대불대학교 2003 論文集 Vol.9 No.1

This study was aimed atclarifying the effects of a Functional Language Intervention in children with language disoders. Five subjects were selected and two speech-language pathologists engaged in the functional language intervention. In the functional language intervention, activities consisted of group time, palying riddles time, following directions time, drawing time, cooking time, word-chain game time, role-playing time, searching for objects based on directions, and recreation game time. To compare pre-treatment with post-treatment in the children with language disorders, repeated-measures ANOVA was used. The results were as follows; First, the Functional Language Intervention was effective in improving the number of communicative intentions of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of communicative intentions. Second, the Functional Language Intervention was effective in improving the number of semantic relations of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of semantic relations. Third, the Functional Language Intervention was effective in improving the overall language abilities of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the overall language abilities.

공정한 키 위탁 시스템에 대한 연구

황보성,이임영 순천향대학교 부설 산업기술연구소 1999 순천향 산업기술연구소논문집 Vol.5 No.1

It is important to manage private keys on large public networks. A Key recovery system can satisfy user key loss or law-enforced access of government. Generally, key recovery system, there are many problems at fairness between user and government. So that, this paper propose new fair key recovery system that can protect of user privacy and user can not pass by key recovery system based on Failsafe and Blind decoding scheme

연폭로 근로자들에서 빈혈증 관련 인자에 관한 연구

황보영,김용배,김화성,이성수,안규동,이병국 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Objectives : This study intended to obtain the interrelationship of lead exposure indices and anemia related factors for the better health management of lead exposed workers. Methods : 502 male workers in storage battery industries as lead exposed group and 106 male office workers as contorl group were selected for this study. The examined variables wer age, work duration, smoking and alcohol crinking habit, blood zinc protoporphyin concentraion (ZPP), blood lead concentration (PbB), urinary δ-aminoevulinic acid (ALAU), hemoglobin (Hb), hematocrit (Hct), serum calcium (S-Ca), serum iron (S-Fe), total iron binding capacity (TIBC), and percentage trasferrin saturation (PTS). Results : The means of ZPP, PbB ALAU in lead exposed proup were 58.4±35.7 ㎍/㎗, 27.6±10.9 ㎍/㎗, and 1.61±2.00 mg/ℓ, respectively. These three values in lead exposed group were significantly higher than control group (P<.05). The means of Hb, Hct, S-Ca, S-Fe, TIBC, PTS in lead exposed group were 15.3±1.1 g/㎗, 47.2±4.5 %, 9.4±0.4 ㎍/㎗, 124.0±67.5 ㎍/㎗, 317.1±68.5 ㎍/㎗, and 38.7±15.2 %, respectively. Hb, Hct, S-Ca, S-Fe, and TIBC in lead exposed group were significantly lower than contrl group (P<.05). PTS was no significant difference in lead exposed group and control group (P> .05). Hct, S-Ca, S-Fe, PTS were significant positive correlation with Hb and ZPP, corrected PbB, AlAU were significant negaitive correlation with Hb in lead exposed group (P< .05). On multiple regression using ZPP as a dependent variable and PbB, S-Ca and S-Fe as independent variables after adjustment for possible confounder such as age, smoking and drinking habit, Pbb, S-Ca, S-Fe and age contributed to ZPP. Conclusions : As the result of this study, it was suggested that inclusion of S-Ca, S-Fe, PTS measurement as a anemia screening test for lead workers was highly recommended for the effective prevention and early diagnosis of anemia.

놀이중심의 집단 언어중재 프로그램이 언어장애 아동의 언어능력에 미치는 영향

황보명 한국동서정신과학회 2003 동서정신과학 Vol.6 No.2

본 연구는 놀이중심의 집단 언어중재 프로그램이 언어장애 아동의 언어능력에 어떤 영향을 미치는지에 대해서 알아보고자 하는 것이다. 본 연구자가 정한 기준에 따라 5명의 언어장애 아동을 연구대상으로 선정하였다. 연구는 사전 단계, 중재 단계, 사후 단계의 세 단계로 나누어 실시하였다. 사전 단계에서는 각 연구대상 아동들의 언어능력 평가를 실시하였는데, 부모와의 인터뷰 실시, 관찰 실시, 언어샘플 수집 등의 하위 단계로 나누어 실시하였다. 중재 단계에서는 놀이중심의 집단 언어중재 프로그램을 연구 대상 아동들에게 주 2회기씩, 총 30회기 동안 제공하였다. 사후 단계에서는 사전 단계와 동일한 언어검사 실시 및 언어샘플을 수집하였다. 본 연구에서 사용한 언어장애 아동의 물리적 환경 및 교육적 환경을 고려하여 본 연구자가 고안한 언어중재 프로그램이다. 이 프로그램에서는 총 10가지의 놀이 활동을 제시하였는데, 이것을 다시 회기마다 포함되는 공통 활동 2가지와 회기마다 변화되는 활동 8가지로 구분하였다. 회기마다 포함되는 공통 활동으로는 자유놀이와 집단 시간이 있고, 회기마다 변화되는 활동들로는 수수께끼, 움직이기, 만들기/그리고, 운동게임, 역할놀이, 끝말잇기, 요리하기, 보물찾기가 있다. 연구 대상에 대한 사전-사후 검사 결과 의사소통 의도 다양성, 의미관계 다양성, 수용언어능력, 표현언어능력, 문장이해능력, 언어이해·인지능력, 그럼어휘능력은 유의미한 차이가 있는 것으로 나타났다. 그러나, 어휘다양도에서는 유의미한 차이가 발견되지 않았다. 따라서 본 연구 결과, 놀이중심의 집단 언어중재 프로그램이 언어장애 아동에게 언어능력 중 화용론 측면과 의미론적 측면뿐만 아니라 전반적인 언어능력을 높이는 데 효과가 있음이 증명되었다. The study was aimed at clarifying the effects of a Group Language Intervention in children with language disorders. Five subjects were selected and two speech-language pathologists engaged in the group language intervention. In the groupnal language intervention, activities consisted of group time, playing riddles time, following directions time, drawing time, cooking time, word-chain game time, role-playing time, searching for objects based on directions, and recreation game time. To compare pre-treatment with post-treatment in the children with language disorders, repeated-measures ANOVA was used. The results were as follows; First, the Functional Language Intervention was effective in improving the number of communicative intentions of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of communicative intentions. Second, the Functional Language Intervention was effective in improving the number of semantic relations of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the number of semantic relations. Third, the Functional Language Intervention was effective in improving the overall language abilities of children with language disorders. There was a significant difference between pre-treatment and post-treatment in the overall language abilities.

副詞的 代名詞 硏究

皇甫淳 慶北大學校 語學硏究所 1980 語文硏究 Vol.5 No.-

韓國企業倫理에 關한 硏究

皇甫太 단국대학교 경영대학원 1981 經院論叢(壇國大學校) Vol.1 No.-