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지중 가스 수송 강관의 차량 이동 속도에 따른 응력 분포 특성
원종화(Jong Hwa Won),김문겸(Moon Kyum Kim),유한규(Han-Kyu Yoo),김미승(Mi Seoung Kim) 한국가스학회 2008 한국가스학회지 Vol.12 No.1
주기적으로 진동이 발생하는 도로 하부에 매설되어 있는 가스 배관의 안정성을 검토하고 동특성을 분석하기 위하여 도로 설계 기준 및 가스공사 배관관리 규정에 의거, 현재 매설 실정을 타당하게 모사할 수 있는 유한요소모델을 구성하였으며, DB-24 하중의 진행속도에 따른 배관 발생 응력분포를 검토하였다. 매설 심도 1.5 m, 차량 이동 속도 40~160 km/h에 대하여 해석을 실시하였으며, 그 결과, 80 km/h를 정점으로 응력의 증가 추세가 감소로 돌아서는 것을 확인할 수 있었다. DB-24하중에 의하여 최대 약 10 Mpa의 응력이 증가 하였으나 설계압력에서 API 5L Gr. X65 매설 배관은 차량의 진동에 대하여 충분한 안정성을 확보하고 있는 것으로 판단된다. In order to estimate the integrity and identify the dynamic characteristics of buried gas pipelines subjected to vehicle loads, FE analysis is performed based on the ‘Highway and Local Road Design Criteria’ and the ‘KOGAS Guideline for Pipeline Management’. The FE model describes the current burial condition of Korea properly, and the DB-24 load model is adopted for this research. This study considers a varying velocity in the range of 40~160 km/h and Pi = 8 Mpa (internal pressure) with depth cover, Z = 1.5 m. Maximum stress occurs at v = 80 km/h and decreases after v = 80 km/h. The maximum induced stress by DB-24 loads is about 10 Mpa. Under the design pressure, however, the analysis results show that API 5L Gr. X65 pipelines have sufficient integrity to withstand the vibration of vehicle loads.
Lee, Geum-Hwa,Ahn, Taeho,Kim, Do-Sung,Park, Seoung Ju,Lee, Yong Chul,Yoo, Wan Hee,Jung, Sung Jun,Yang, Jae-Seong,Kim, Sanguk,Muhlrad, Andras,Seo, Young-Rok,Chae, Soo-Wan,Kim, Hyung-Ryong,Chae, Han-Jun American Society for Microbiology 2010 Molecular and cellular biology Vol.30 No.7
<B>ABSTRACT</B><P>Bax inhibitor 1 (BI-1), a transmembrane protein with Ca<SUP>2+</SUP> channel-like activity, has antiapoptotic and anticancer activities. Cells overexpressing BI-1 demonstrated increased cell adhesion. Using a proteomics tool, we found that BI-1 interacted with γ-actin via leucines 221 and 225 and could control actin polymerization and cell adhesion. Among BI-1<SUP>−/−</SUP> cells and cells transfected with BI-1 small interfering RNA (siRNA), levels of actin polymerization and cell adhesion were lower than those among BI-1<SUP>+/+</SUP> cells and cells transfected with nonspecific siRNA. BI-1 acts as a leaky Ca<SUP>2+</SUP> channel, but mutations of the actin binding sites (L221A, L225A, and L221A/L225A) did not change intra-endoplasmic reticulum Ca<SUP>2+</SUP>, although deleting the C-terminal motif (EKDKKKEKK) did. However, store-operated Ca<SUP>2+</SUP> entry (SOCE) is activated in cells expressing BI-1 but not in cells expressing actin binding site mutants, even those with the intact C-terminal motif. Consistently, actin polymerization and cell adhesion were inhibited among all the mutant cells. Compared to BI-1<SUP>+/+</SUP> cells, BI-1<SUP>−/−</SUP> cells inhibited SOCE, actin polymerization, and cell adhesion. Endogenous BI-1 knockdown cells showed a similar pattern. The C-terminal peptide of BI-1 (LMMLILAMNRKDKKKEKK) polymerized actin even after the deletion of four or six charged C-terminal residues. This indicates that the actin binding site containing L221 to D231 of BI-1 is responsible for actin interaction and that the C-terminal motif has only a supporting role. The intact C-terminal peptide also bundled actin and increased cell adhesion. The results of experiments with whole recombinant BI-1 reconstituted in membranes also coincide well with the results obtained with peptides. In summary, BI-1 increased actin polymerization and cell adhesion through Ca<SUP>2+</SUP> regulation and actin interaction.</P>
대동맥판막 치환술 환자에서 발생한 아스페르길루스 심내막염 1예
신재령 ( Jae Ryung Shin ),김승민 ( Seung Min Kim ),유정선 ( Jung Sun Yoo ),오치혁 ( Chi Hyuk Oh ),정명화 ( Myoung Hwa Jung ),박지윤 ( Ji Yoon Park ),문수연 ( Soo Youn Moon ),손준성 ( Jun Seoung Son ),이미숙 ( Soo Cheol Kim ),김수 전북대학교 의과학연구소 2013 全北醫大論文集 Vol.37 No.1
아스페르길루스 심내막염은 국내에서도 7증례만 보고된 바 있는 드문 질환이며 명확한 치료 방안이 정립되지 못하여 사망률 또한 매우 높은 질환이다. 최근 심장수술의 증가, 장기이식, 광범위 항생제 사용 등으로 입원환자 중에 그 발생빈도가 증가하고 있어 해당 질환에 대한 관심이 필요하겠다. 현재까지 아스페르길루스 심내막염의 치료는 외과적 수술 및 적절한 항균제의 사용이 고려되고 있으나 병합요법의 필요성 및 추후 이차적 예방에 관해 더 많은 연구가 요구되고 있다. 저자들은 대동맥판막 치환술및 관상동맥 우회술을 시행한 환자에서 판막파열 및 가성동맥류가 발생하였고, 수술 후 조직검사에서 A. fumigatus를 확인했던 아스페르길루스 심내막염 환자를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Aspergillus endocarditis is a rare infection that occurs most commonly after heart valve replacement surgery. The outcomes for patients with prosthetic valve endocarditis due to Aspergillus species have been extremely poor. Therapeutic management conventionally consists of combined medical and surgical approaches. However, there was no clear evidence to determine whether or not combination therapy was superior to liposomal amphotericin B alone. And no clear pattern could be determined in relation to the optimal duration of initial antifungal treatment or of long-term secondary prophylaxis. We report a case of aspergillus endocarditis in a patient who underwent aortic valve replacement surgery. The results of repeated blood culture were negative. Aortic root replacement was performed and Aspergillus fumigatus was isolated from aortic valve tissue. Despite of postoperative liposomal amphotericin B therapy, he died of multiple organ failure associated with diffuse brain damage.
Shin, Yong-Joo,Han, Song-Hee,Kim, Do-Sung,Lee, Geum-Hwa,Yoo, Wan-Hee,Kang, Yong-Mo,Choi, Je-Yong,Lee, Yong Chul,Park, Seoung Ju,Jeong, Seul-Ki,Kim, Hyung-Tae,Chae, Soo-Wan,Jeong, Hyun-Ja,Kim, Hyung-Ry BioMed Central 2010 ARTHRITIS RESEARCH AND THERAPY Vol.12 No.1
<P><B>Introduction</B></P><P>Synovial fibroblasts from rheumatoid arthritis show resistance to apoptotic stimuli, indicating they may be difficult to treat. To clearly understand these mechanisms of resistance, rheumatoid and osteoarthritis synovial fibroblasts (RASF and OASF) were exposed to endoplasmic reticulum (ER) stress such as thapsigargin, Ca<SUP>2+</SUP>-ATPase inhibitor.</P><P><B>Methods</B></P><P>Fibroblasts were assessed microscopically for cell viability by trypan blue exclusion and for autophagic cells by LC-3II formation. Caspase-3 activity was measured as aminomethyl-coumarin (AMC) liberated from AC-DEVD-AMC. Immunoblotting was performed to compare protein expression in OASF and RASF.</P><P><B>Results</B></P><P>ER stress caused cell death in OASF but not in RASF. Thapsigargin, a Ca<SUP>2+</SUP>-ATPase inhibitor, did not change the expression of GRP78, an ER chaperone in OASF and RASF, but induced another ER stress protein, CCAAT/enhancer binding protein (C/EBP) homologous protein (CHOP) differently, showing high levels in OASF and low levels in RASF. Thapsigargin increased the autophagy response in RASF, with autophagosome formation, beclin expression, and LC3-II conversion. Transfection with beclin siRNA inhibited autophagy and increased the susceptibility to ER stress-induced cell death. On the other hand, CHOP siRNA increased autophagy and improved cell survival, especially in RASF, indicating that CHOP is involved in regulation of autophagy and cell death, but that low expression of CHOP protects RASF from apoptosis.</P><P><B>Conclusions</B></P><P>Autophagy induction and CHOP under-expression increases cell resistance against ER stress-induced cell death in fibroblasts from rheumatoid arthritis patients.</P>
( Eun Ji Kim ),( Bo Ram Kim ),( Jung Won Cho ),( Jung Hwa Lee ),( Eun Sook Lee ),( Yun Mi Yu ),( Ho-seoung Han ),( Yoo Seok Yoon ),( Jai Young Choi ),( Euni Lee ),( Youngrok Choi ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Tacrolimus has a narrow therapeutic range requiring concentration monitoring. The purpose of this study is to evaluate the effect of tacrolimus intrapatient variability (IPV, %) on clinical outcomes in liver transplantation. Methods: A retrospective analysis using electronic medical records of liver transplant recipients from 2009 to 2016 was conducted in Seoul National University Bundang Hospital. IPV was calculated by using individual tacrolimus concentration and averaged tacrolimus concentration. Patients were divided into two groups based on the median IPV; high variability (HV) and low variability (LV) groups. The rate of deviation from the therapeutic range, the incidence of acute rejection, and post-transplant diabetes, the number of infection and estimated glomerular filtration rate (eGFR) within 6 months after transplantation were compared between two groups. Results: Of 82 study subjects, HV group (17.3%) has more frequently deviated from the therapeutic range than the LV group (4.5%) (P<0.001). There was no significant difference in the incidence of acute rejection, post-transplantation diabetes and eGFR in both groups, but the number of infection was significantly lower in LV group (0.4 times) than in HV group (0.9 times) for 6 months (P=0.039). The multivariate regression analysis showed that the number of infection was significantly increased as the IPV increased (P=0.003). Conclusions: We compared the clinical outcomes between HV and LV groups within 6 months after liver transplantation. High IPV of tacrolimus concentration was strongly associated with increased frequency of deviation from the therapeutic range and the number of infection.