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Hui Tan,Hui Ling,Jie He,Lan Yi,Jianguo Zhou,Min Lin,Qi Su 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6
We investigated the effects of diallyl disulfide (DADS) on the induction of apoptosis in human Leukemia cell line HL-60 and explored the roles of mitogen-activated protein kinase (ERK and p38 MAPK) pathways in the growth inhibition and apoptosis induced by DADS. MTT assay was used to determine the DADS induced cell growth inhibition in HL-60 cells. Flow cytometry and DNA fragmentation were used to examine the roles of apoptosis in DADS-mediated cell death. Western blot analysis of the expression of phospho-MAPKs (ERK and p38) was employed to elucidate the possible mechanisms of DADS induced apoptosis. We found that growth inhibition of HL-60 cells treated with DADS exhibited a dose-dependent response (P<0.05) and DADS induced significant apoptosis. DADS at the concentration of 10 mg/L persistently activated p38 and simultaneously reduced ERK activity. PD98059, an inhibitor of ERK upstream activators MAPK kinase MKK1 and MKK2, promoted cytotoxicity and apoptosis in HL-60 cells treated with DADS. In contrast, SB203580, an inhibitor of p38, decreased cytotoxicity and apoptosis induced by DADS. Therefore, DADS can effectively inhibit the proliferation and induce apoptosis of human leukemia cell line HL-60. Inhibition of ERK signaling pathways and activation of p38 signaling pathways are likely involved in DADS induced apoptosis in HL- 60 cells.
Tan, Hui,Ling, Hui,He, Jie,Yi, Lan,Zhou, Jianguo,Lin, Min,Su, Qi 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.6
We investigated the effects of diallyl disulfide (DADS) on the induction of apoptosis in human Leukemia cell line HL-60 and explored the roles of mitogen-activated protein kinase (ERK and p38 MAPK) pathways in the growth inhibition and apoptosis induced by DADS. MTT assay was used to determine the DADS induced cell growth inhibition in HL-60 cells. Flow cytometry and DNA fragmentation were used to examine the roles of apoptosis in DADS-mediated cell death. Western blot analysis of the expression of phospho-MAPKs (ERK and p38) was employed to elucidate the possible mechanisms of DADS induced apoptosis. We found that growth inhibition of HL-60 cells treated with DADS exhibited a dose-dependent response (P<0.05) and DADS induced significant apoptosis. DADS at the concentration of 10 mg/L persistently activated p38 and simultaneously reduced ERK activity. PD98059, an inhibitor of ERK upstream activators MAPK kinase MKK1 and MKK2, promoted cytotoxicity and apoptosis in HL-60 cells treated with DADS. In contrast, SB203580, an inhibitor of p38, decreased cytotoxicity and apoptosis induced by DADS. Therefore, DADS can effectively inhibit the proliferation and induce apoptosis of human leukemia cell line HL-60. Inhibition of ERK signaling pathways and activation of p38 signaling pathways are likely involved in DADS induced apoptosis in HL-60 cells.
간세포암에서 임상병리학적 특성에 따른 Apolipoprotein (apoA-1, apoL-1)의 발현양상
김인희 ( Kim In Hui ),박정향 ( Park Jeong Hyang ),유경란 ( Yu Gyeong Lan ),조백환 ( Jo Baeg Hwan ),문우성 ( Mun U Seong ),이승옥 ( Lee Seung Og ),이수택 ( Lee Su Taeg ),김대곤 ( Kim Dae Gon ),안득수 ( An Deug Su ) 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-
<목적> 혈청 apolipoprotein A-I은 담즙울체성 간질환, 알콜성 간질환, 간섬유화 및 간경변증에 대한 좋은 지표이다. 본 연구에서는 간세포암과 그 주위 조직에서 apolipoprotein (apoA-I, apoL-I, apoM)의 발현양상을 분석하여 간세포암의 발생과정에서의 이들의 역할을 알아보고자 한다. <대상 및 방법> 간세포암으로 수술 받은 43명의 환자들로부터 얻은 간세포암과 그 주위 조직 내에서 apoA-I, apoL-I, apoM
Genotypespecific methylation of HPV in cervical intraepithelial neoplasia
Yaw-Wen Hsu,Rui-Lan Huang,Po-Hsuan Su,Yu-Chih Chen,Hui-Chen Wang,Chi-Chun Liao,Hung-Cheng Lai 대한부인종양학회 2017 Journal of Gynecologic Oncology Vol.28 No.4
Objective: Hypermethylation of human papillomavirus (HPV) and host genes has beenreported in cervical cancer. However, the degree of methylation of different HPV typesrelative to the severity of the cervical lesions remains controversial. Studies of the degree ofmethylation associated with the host gene and the HPV genome to the severity of cervicallesions are rare. We examined the association of methylation status between host genes andlate gene 1 (L1) regions of HPV16, 18, 52, and 58 in cervical brushings. Methods: Cervical brushings from 147 HPV-infected patients were obtained. The samplescomprised normal (n=28), cervical intraepithelial neoplasia (CIN) 1 (n=45), CIN2 (n=13), andCIN3/carcinoma in situ (n=61). The methylation status of HPV and host genes was measuredusing bisulfite pyrosequencing and quantitative methylation-specific polymerase chainreaction (PCR). Results: The degree of methylation of L1 in HPV16, 18, and 52 was associated with theseverity of the cervical lesion. In HPV52, C-phosphate-G (CpG) sites 6368m, 6405m, and6443m showed significantly higher methylation in lesions ≥CIN3 (p=0.005, 0.003, and0.026, respectively). Methylation of most HPV types except HPV52 (r<−0.1) was positivelycorrelated with the degree of methylation of host genes including PAX1 and SOX1 (0.4≤r≤0.7). Combining HPV methylation with PAX1 methylation improved the clustering for ≥CIN2. Conclusion: Our study showed that the degree of L1 methylation of HPV16, 18, and 52but not 58 is associated with the severity of cervical lesions. The association betweenHPV methylation and host gene methylation suggests different responses of host cellularepigenetic machinery to different HPV genotypes.
상복부 자기자극(magnetic stimulation)이 피부 위전도에 미치는 영향
김현정 ( Kim Hyeon Jeong ),이준성 ( Lee Jun Seong ),임희혁 ( Im Hui Hyeog ),황경란 ( Hwang Gyeong Lan ),정인섭 ( Jeong In Seob ),고봉민 ( Go Bong Min ),홍수진 ( Hong Su Jin ),유창범 ( Yu Chang Beom ),김진오 ( Kim Jin O ),조주영 ( 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-
<목적> 위마비증의 치료로 개발된 위전기자극법은 주파수에 따라 위전기 박동(GEP), 고주파수 위전기자극(HI-GES), 신경 위전기자극(NEGS)이 있다. GEP나 HI-GES는 위의 수축 및 배출에는 거의 영향이 없고, NEGS의 경우 동물에서 콜린성 신경자극 및 아세틸콜린 유리로 위를 수축하지만 인체에서는 위 수축 및 배출 효과, 수축을 결정하는 주파수를 잘 모른다. 최근 개발된 자기자극기는 전기자극과 달리 비교적 안전하며 투과 깊이가 깊어 비침습
호소형 및 하천형 댐 호의 육수학적 특성과 조류발생과의 상관관계
김종민 ( Kim Jong Min ),허성남 ( Heo Seong Nam ),노혜란 ( Noh Hye Lan ),양희정 ( Yang Hui Jeong ),한명수 ( Han Myeong Su ) 한국하천호수학회 2003 생태와 환경 Vol.36 No.2
This paper aimed to analyze the relationship between algal bloom patterns and hydrological, limnological data which were collected from major reservoirs in Korea for 8 years (1990~1997). Water temperature of river-type reservoirs showed wider seasonal fluctuations than that of lake-type. pH of lake-type reservoirs was low in winter season but high in summer season. In contrast, river-type reservoirs showed high pH in spring and autumn seasons as well, and very low in summer season. COD of lake-type reservoirs and Paldang reservoir was lower (2~3 mg/L) than that of Geumgang and Nagdonggang reservoirs (6~9 mg/L). Dissolved oxygen (DO) of river-type reservoirs was higher than that of lake-type reservoirs. Seasonal fluctuation pattern of Do saturation in river-type reservoirs was high (80~100%) and remained relatively constant whereas lake-type reservoirs showed the highest level (93%) in late spring or early summer, which gradually decreased entering winter season (46~66%). And monthly variation of DO saturation showed inverse proportion to water volume in lake-type reservoirs. Nutrients concentration in river-type lake is higher than lake-type. Seasonal fluctuation of nutrients (T-N, T-P) in lake-type reservoirs was relatively small than that of river-type reservoirs. Annual mean N/P mass ratio of lake-type reservoirs was higher than that of river-type. Transparency tended to related with the suspended solid concentration in river-type reservoirs. Algal bloom of lake-type and river-type reservoirs occurred at any time except rainfall and winter periods. And it dominated in summer and early autumn, respectively. Algal bloom of river-type reservoirs was higher than that of lake-type. Relationship between rainfall and chlorophyll-α in lake-type reservoirs was relatively high, however river-type reservoirs showed insignificant.
위 식도 역류 질환에서 공동관(common cavity)의 임상적 가치에 대한 연구
김성환 ( Kim Seong Hwan ),이준성 ( Lee Jun Seong ),임희혁 ( Im Hui Hyeog ),황경란 ( Hwang Gyeong Lan ),정인섭 ( Jeong In Seob ),고봉민 ( Go Bong Min ),홍수진 ( Hong Su Jin ),유창범 ( Yu Chang Beom ),김진오 ( Kim Jin O ),조주영 ( J 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-
<목적> 위식도 역류의 식도 내압검사 상 표지자로 공동관(common cavity)이 알려져 있으나 아직 이에 대한 연구가 많지 않고 임상적으로 거의 사용되고 있지 않다. 본 연구의 목적은 1) 위 식도 역류시 공동관 동반 유무를 알아보며, 기립시, 앙와위, 식후에 차이가 있는지 조사해보고, 2) 공동관 횟수로 위 식도 역류질환(GERD)을 예측할 수 있는 지 그 진단적 가치를 알아보고자 하였다. <방법> 24시간 보행성 식도 pH 검사와 보행성 식도내