Separation of Nattokinase from Bacillus subtilis Fermentation Broth by Expanded Bed Adsorption with Mixed-mode Adsorbent

Shan-Jing Yao,Miao-Hua Lu,Dong-Qiang Lin,Yuan-Chun Wu,Jun-Xian Yun,Le-He Mei 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2

Mixed-mode hydrophobic/ionic matrices exhibit a salt-tolerant property for adsorbing target protein from high-ionic strength feedstock, which allows the application of undiluted feedstock via an expanded bed process. In the present work, a new type of mixed-mode adsorbent designed for expanded bed adsorption, Fastline PRO, was challenged for the capture of nattokinase from the high ionic fermentation broth of Bacillus subtilis. Two important factors, pH and ion concentration, were investigated with regard to the performance of nattokinase adsorption. Under initial fermentation broth conditions (pH 6.6 and conductivity of 10 mS/cm) the adsorption capacity of nattokinase with Fastline PRO was high, with a maximum capacity of 5,350 U/mL adsorbent. The elution behaviors were investigated using packed bed adsorption experiments, which demonstrated that the effective desorption of nattokinase could be achieved by effecting a pH of 9.5. The biomass pulse response experiments were carried out in order to evaluate the biomass/adsorbent interactions between Bacillus subtilis cells and Fastline PRO, and to demonstrate a stable expanded bed in the feedstock containing Bacillus subtilis cells. Finally, an EBA process, utilizing mixed-mode Fastline PRO adsorbent, was optimized to capture nattokinase directly from the fermentation broth. The purification factor reached 12.3, thereby demonstrating the advantages of the mixed-mode EBA in enzyme separation.

On the Feasibility of Interference Alignment in the Cellular Network

( Hua Chen ),( Shan Wu ),( Ping Hu ),( Zhudi Xu ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.11

In this paper, we investigate the feasibility of interference alignment(IA) in signal space in the scenario of multiple cell and multiple user cellular networks, as the feasibility issue is closely related to the solvability of a multivariate polynomial system, we give the mathematical analysis to support the constraint condition obtained from the polynomial equations with the tools of algebraic geometry, and a new distribute IA algorithm is also provided to verify the accessibility of the constraint condition for symmetric system in this paper. Simulation results illustrate that the accessibility of the constraint condition is hold if and only if the degree of freedom(DoF) of each user can be divided by both the transmit and receive antenna numbers.

Evaluation of Fatigue Damage in Steel Plates Through Non-linear Ultrasonic Technique

Che-Hua Yang,N. Jeyaprakash,Shan-Yan Wu 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.5

Under the action of repeated stress on the components such as turbine blades, springs, crankshafts, biomedical transplantation, and load components, the strength of the material is reduced or the material is damaged. These components continue to be subjected to different types of tension, compression, bending, vibration, thermal expansion, and contraction or other repetitive stress effects. These stresses are often lower than the yield strength of the material, but must be greater than the fatigue limit of the material. The stress can cause the dislocation substructure of the material, thereby changing fatigue failure. In this study, we mainly used non-linear ultrasonic measurement to generate high-energy waves, made it incident on the steel and received its double-frequency signal at the other end. Then we calculated the non-linear factor of steel based on the ratio of the double frequency amplitude to the square of the fundamental frequency amplitude. Besides, the non-linear factor of steel was measured under different fatigue cycles. Then, the curve of the material non-linear factor was determined and the cycle stress number was measured by the relationship between different fatigue cycles and their non-linear factors. The final results show that, with the increase in the number of applied cyclic stresses, the non-linear factor also tends to increase. The non-linear factor of the test piece with no damage at all and the test piece with one million fatigue damages can be up to 6.5%. Non-linear ultrasonic technique was successfully implemented to analyse the fatigue damages.

Separation of Nattokinase from Bacillus subtilis Fermentation Broth by Expanded Bed Adsorption with Mixed-mode Adsorbent

Lu Miao-Hua,Lin Dong-Qiang,Wu Yuan-Chun,Yun Jun-Xian,Mei Le-He,Yao Shan-Jing The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2

Mixed-mode hydrophobic/ionic matrices exhibit a salt-tolerant property for adsorbing target protein from high-ionic strength feedstock, which allows the application of undiluted feedstock via an expanded bed process. In the present work, a new type of mixed-mode adsorbent designed for expanded bed adsorption, Fastline $PRO^{\circledR}$, was challenged for the capture of nattokinase from the high ionic fermentation broth of Bacillus subtilis. Two important factors, pH and ion concentration, were investigated with regard to the performance of nattokinase ad-sorption. Under initial fermentation broth conditions (pH 6.6 and conductivity of 10 mS/cm) the adsorption capacity of nattokinase with Fastline PRO was high, with a maximum capacity of 5,350 U/mL adsorbent. The elution behaviors were investigated using packed bed adsorption experiments, which demonstrated that the effective desorption of nattokinase could be achieved by effecting a pH of 9.5. The biomass pulse response experiments were carried out in order to evaluate the biomass/adsorbent interactions between Bacillus subtilis cells and Fastline PRO, and to demonstrate a stable expanded bed in the feedstock containing Bacillus subtilis cells. Finally, an EBA process, utilizing mixed-mode Fastline PRO adsorbent, was optimized to capture nattokinase directly from the fermentation broth. The purification factor reached 12.3, thereby demonstrating the advantages of the mixed-mode EBA in enzyme separation.

CEA, AFP, CA125, CA153 and CA199 in Malignant Pleural Effusions Predict the Cause

Wang, Xin-Feng,Wu, Yan-Hua,Wang, Mao-Shui,Wang, Yun-Shan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

Determination of the cause of malignant pleural effusions is important for treatment and management, especially in cases of unknown primaries. There are limited biomarkers available for prediction of the cause of malignant pleural effusion in clinical practice. Hence, we evaluated pleural levels of five tumor biomarkers (CEA, AFP, CA125, CA153 and CA199) in predicting the cause of malignant pleural effusion in a retrospective study. Kruskal-Wallis or Mann-Whitney U tests were carried out to compare levels of tumor markers in pleural effusion among different forms of neoplasia - lung squamous cell carcinoma, adenocarcinoma, or small cell carcinoma, mesothelioma, breast cancer, lymphoma/leukemia and miscellaneous. Receiver operator characteristic analysis was performed to evaluate sensitivity and specificity of biomarkers. The Kruskal-Wallis test showed significant differences in levels of pleural effusion CEA (P<0.01), AFP (P<0.01), CA153 (P<0.01) and CA199 (P<0.01), but not CA125 (P>0.05), among the seven groups. Receiver operator characteristic analysis showed that, compared with other four tumor markers, CA153 was the best biomarker in diagnosing malignant pleural effusions of lung adenocarcinoma (area under curve (AUC): 0.838 (95%confidence interval: 0.787, 0.888); cut-off value: 10.2U/ml; sensitivity: 73.2% (64.4-80.8)%, specificity: 85.2% (77.8-90.8)%), lung squamous cell carcinoma (AUC: 0.716 (0.652, 0.780); cut-off value: 14.2U/ml; sensitivity: 57.6% (50.7-64.3)%, specificity: 91.2% (76.3-98.0)%), and small-cell lung cancer (AUC: 0.812 (0.740, 0.884); cut-off value: 9.7U/ml; sensitivity: 61.5% (55.0-67.8)%, specificity: 94.1% (71.2-99.0)%); CEA was the best biomarker in diagnosing MPEs of mesothelioma (AUC: 0.726 (0.593, 0.858); cut-off value: 1.43ng/ml; sensitivity: 83.7% (78.3-88.2)%, specificity: 61.1% (35.8-82.6)%) and lymphoma/leukemia (AUC: 0.923 (0.872, 0.974); cut-off value: 1.71ng/ml; sensitivity: 82.8% (77.4-87.3)%, specificity: 92.3% (63.9-98.7)%). Thus CA153 and CEA appear to be good biomarkers in diagnosing different causes of malignant pleural effusion. Our findings implied that the two tumor markers may improve the diagnosis and treatment for effusions of unknown primaries.

HMGB1 regulates autophagy through increasing transcriptional activities of JNK and ERK in human myeloid Leukemia cells

( Ming Yi Zhao ),( Ming Hua Yang ),( Liang Chun Yang ),( Yan Yu ),( Min Xie ),( Shan Zhu ),( Rui Kang ),( Dao Lin Tang ),( Zhi Gang Jiang ),( Wu Zhou Yuan ),( Xiu Shan Wu ),( Li Zhi Cao ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.9

HMGB1 is associated with human cancers and is an activator of autophagy which mediates chemotherapy resistance. We here show that the mRNA levels of HMGB1 are high in leukemia cells and it is involved in the progression of childhood chronic myeloid leukemia (CML). HMGB1 decreases the sensitivity of human myeloid leukemia cells K562 to anti-cancer drug induced death through up-regulating the autophagy pathway, which is confirmed by the observation with an increase in fusion of autophagosomes and autophagolysosomes. When overexpressing HMGB1, both mRNA levels of Beclin-1, VSP34 and UVRAG which are key genes involved in mammalian autophagy and protein levels of p-Bcl-2 and LC3-II are increased. Luciferase assays document that over-expression of HMGB1 increases the transcriptional activity of JNK and ERK, which may be silenced by siRNA. The results suggest that HMGB1 regulates JNK and ERK required for autophagy, which provides a potential drug target for therapeutic interventions in childhood CML. [BMB reports 2011; 44(9): 601-606]

온라인 협업 도구 활용 한국어 수업 사례 : 상해 삼달대학교를 중심으로

배영련(Pei, Ying Lian),오선자(Wu, Shan Zi),최선진(Choi, Seon Jin),한나(Han, Na),오영화(Wu, Ying Hua) 글로벌교육연구학회 2021 글로벌교육연구 Vol.13 No.4

본 연구는 온라인 수업에서의 상호작용에 도움을 주며, 중국과 한국에서 동시에 사용할수 있는 다양한 온라인 협업 도구를 소개하고, 이 도구 네 가지(mentimeter, zoom, padlet, Quizizz)를 사용하여 중국인 대상 한국어 수업을 운영한 사례를 제시하였다. 그리고본 수업 운영에 대한 학습자 만족도를 살펴보기 위하여 2021학년도 3월 학기에 본 수업을수강한 상해 삼달대학교 한국어 학습자 75명을 대상으로 만족도 조사를 시행하였으며, 그결과 전체적인 수업 만족도는 평균 4.09였다. 또한, 온라인 수업에서의 상호작용 향상을위한 방법으로 활용한 온라인 협업 도구에 대한 전체적인 만족도는 4.24였으며, 각 도구의만족도는 mentimeter가 4.63, zoom이 4.15, padlet이 4.09, Quizizz 3.81 순이었다. 이 중에서 협업 도구에 대한 전체적인 만족도와 padlet 만족도가 수업 전체 만족도에 영향을미치는 것으로 분석되었다. 본 연구 결과에 따른 제언은 다음과 같다. 첫째, 온라인 협업도구 활용에 대한 학습자 만족도가 높기 때문에 한국어 수업에 활용할 수 있는 다양한 협업도구 연구를 바탕으로 수업 적용이 필요하며, 각 국 학습자용 교재 편찬이 필요하다고 할수 있다. 본 연구는 다양한 온라인 수업에서의 상호작용에 필요한 다양한 협업 도구를 소개하고 비대면 수업에서의 실제 활용방안을 제시했으며, 이에 대한 학습자들의 의견을 수렴했다는 점에서 의의가 있다. This study was to present a case of an Online Korean class using four online tools such as Mentimeter, Zoom, Padlet, Quizizz for helping the interaction of Korean and Chinese students. To investigate learner satisfaction, 75 Chinese students from Shanda University participated in a survey after the class. The results were as follows: First, the overall class satisfaction was 4.09, especially students were satisfied with using collaboration tools. Second, online tool satisfaction was 4.63 for Mentimeter, 4.15 for Zoom, 4.09 for Padlet, and 3.81 for Quizizz in the order of the program. Based on the results, it was suggested applications of various online tools into class and textbooks for learners. At last, it was significant that the case of online Korean class was presented with many online tools and learners satisfaction in this study.

Synergistic efficacy of LBH and αB-crystallin through inhibiting transcriptional activities of p53 and p21

( Yun Deng ),( Yong Qing Li ),( Xiong Wei Fan ),( Wu Zhou Yuan ),( Hua Ping Xie ),( Xiao Yang Mo ),( Yan Yan ),( Jun Mei Zhou ),( Yue Qun Wang ),( Xian Li Ye ),( Yong Qi Wan ),( Xiu Shan Wu ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.6

LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes αB-crystallin. Co-immunoprecipitation and GST pull-down assays showed that LBH interacts with αB-crystallin, which is further confirmed by mammalian two-hybrid assays. Co-localization analysis showed that in COS-7 cells, αB-crystallin that is cytoplasmic alone, accumulates partialy in the nucleus when co-transfected with LBH. Transient transfection assays indicated that overexpression of LBH or αB-crystallin reduced the transcriptional activities of p53 and p21, respectively, Overexpression of both αB-crystallin and LBH together resulted in a stronger repression of the transcriptional activities of p21 and p53. These results showed that the interaction of LBH and αB-crystallin may inhibit synergistically the transcriptional regulation of p53 and p21. [BMB reports 2010; 43(6): 432-437]

A novel human KRAB-related zinc finger gene ZNF425 inhibits mitogen-activated protein kinase signaling pathway

( Yue Qun Wang ),( Xiang Li Ye ),( Jun Mei Zhou ),( Yong Qi Wan ),( Hua Ping Xie ),( Yun Deng ),( Yan Yan ),( Yong Qing Li ),( Xiong Wei Fan ),( Wu Zhou Yuan ),( Xiao Yang Mo ),( Xiu Shan Wu ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.1

Zinc finger (ZNF) proteins play a critical role in cell growth, proliferation, apoptosis, and intracellular signal transduction. In this paper, we cloned and characterized a novel human KRAB-related zinc finger gene, ZNF425, which encodes a protein of 752 amino acids. ZNF425 is strongly expressed in the three month old human embryos and then is almost undetectable in six month old embryos and in adult tissues. An EGFP-ZNF425 fusion protein can be found in both the nucleus and the cytoplasm. ZNF425 appears to act as a transcription repressor. Over-expression of ZNF425 inhibits the transcriptional activities of SRE, AP-1, and SRF. Deletion analysis indicates that the C2H2 domain is the main region responsible for the repression. Our results suggest that the ZNF425 gene is a new transcriptional inhibitor that functions in the MAPK signaling pathway. [BMB reports 2011; 44(1): 58-63]

The Relationships among Symptom Distress, Hope and Self-care in Breast Cancer Patients with Chemotherapy after Mastectomy

Chiu-Yueh Hsiao,Shu-Hui Yeh,Lina- Lee,Shu-Fen Lan,Hua-Shan Wu 한국간호과학회 2009 한국간호과학회 학술대회 Vol.2009 No.10