The F-box Protein KIB1 Mediates Brassinosteroid-Induced Inactivation and Degradation of GSK3-like Kinases in <i>Arabidopsis</i>

Zhu, Jia-Ying,Li, Yuyao,Cao, Dong-Mei,Yang, Hongjuan,Oh, Eunkyoo,Bi, Yang,Zhu, Shengwei,Wang, Zhi-Yong Elsevier 2017 Molecular cell Vol.66 No.5

<P><B>Summary</B></P> <P>The glycogen synthase kinase-3 (GSK3) family kinases are central cellular regulators highly conserved in all eukaryotes. In <I>Arabidopsis</I>, the GSK3-like kinase BIN2 phosphorylates a range of proteins to control broad developmental processes, and BIN2 is degraded through unknown mechanism upon receptor kinase-mediated brassinosteroid (BR) signaling. Here we identify KIB1 as an F-box E3 ubiquitin ligase that promotes the degradation of BIN2 while blocking its substrate access. Loss-of-function mutations of KIB1 and its homologs abolished BR-induced BIN2 degradation and caused severe BR-insensitive phenotypes. KIB1 directly interacted with BIN2 in a BR-dependent manner and promoted BIN2 ubiquitination in vitro. Expression of an F-box-truncated KIB1 caused BIN2 accumulation but dephosphorylation of its substrate BZR1 and activation of BR responses because KIB1 blocked BIN2 binding to BZR1. Our study demonstrates that KIB1 plays an essential role in BR signaling by inhibiting BIN2 through dual mechanisms of blocking substrate access and promoting degradation.</P> <P><B>Highlights</B></P> <P> <UL> <LI> KIB1 is an essential positive regulator in brassinosteroid signaling </LI> <LI> KIB1 mediates BR-induced ubiquitination and degradation of GSK3 kinase BIN2 </LI> <LI> KIB1 binding to BIN2 prevents BIN2-substrate interaction and promotes BIN2 degradation </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

Static and free vibration analysis of shallow sagging inclined cables

Li, Zhi-Jiang,Li, Peng,He, Zeng,Cao, Ping Techno-Press 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.45 No.2

Based on link-model, we conducted a static analysis and computation of a three-span suspended cable structure in the present paper, and obtained the static configuration and tension distribution of the cable. Using the link and beam model based on finite element method, we analyzed the vibration modal of three-span suspended cable structure, and compared with the results obtained from ANSYS using link and beam element. The vibration modals of shallow sagging inclined cables calculated from proposed method agrees well with ANSYS results, which validates the proposed method. As a result, the influence of bend stiffness on in-plane natural frequencies is much greater than that on out-of-plane natural frequencies of inclined cables.

Static and free vibration analysis of shallow sagging inclined cables

Zhi-Jiang Li,Peng Li,Zeng He,Ping Cao 국제구조공학회 2013 Structural Engineering and Mechanics, An Int'l Jou Vol.45 No.2

Based on link-model, we conducted a static analysis and computation of a three-span suspended cable structure in the present paper, and obtained the static configuration and tension distribution of the cable. Using the link and beam model based on finite element method, we analyzed the vibration modal of three-span suspended cable structure, and compared with the results obtained from ANSYS using link and beam element. The vibration modals of shallow sagging inclined cables calculated from proposed method agrees well with ANSYS results, which validates the proposed method. As a result, the influence of bend stiffness on in-plane natural frequencies is much greater than that on out-of-plane natural frequencies of inclined cables.

Mixtures of Tea and Citrus maxima (pomelo) Alleviate Lipid Deposition in HepG2 Cells Through the AMPK/ACC Signaling Pathway

Ran An,Shuai Wen,Dong-Li Li,Qiu-Hua Li,Xing-Fei Lai,Wen-Ji Zhang,Ruo-Hong Chen,Jun-Xi Cao,Zhi-Gang Li,Qiu-Sheng Huang,Ling-Li Sun,Shi-Li Sun 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.9

Tea and citrus maxima are natural, medicinal homologous plants, typically used for making beverages, which have anticancer, antiobesity, and antioxidation properties. Green tea, yellow tea, and black tea were combined with citrus maxima to obtain green tea and Citrus maxima (GTCM), yellow tea and Citrus maxima (YTCM), and black tea and Citrus maxima (BTCM). The biochemical components of these mixtures were analyzed, and their possible effects and mechanisms on relieving liver lipid deposition were explored. The tea polyphenols, free amino acids, phenolamine ratio, and caffeine were comparable in YTCM and GTCM, being significantly higher than those in BTCM. In addition, the content of esterified catechins, nonesterified catechins, and total catechins in YTCM was significantly higher than those in GTCM and BTCM. All three mixtures of Citrus maxima tea significantly reduced lipid deposition in HepG2 cells, with GTCM and YTCM being slightly more effective than BTCM. Regarding the possible mechanism, Western blot analysis revealed that the three Citrus maxima tea mixtures could activate the AMPK/ACC signaling pathway, upregulate the expression of p-AMPK, p-ACC, and CPT-1 proteins, and downregulate the expression of SREBP1c and fatty acid synthase proteins to inhibit fat synthesis, thereby relieving lipid deposition in liver cells. In conclusion, as a novel and healthy beverage, Citrus maxima tea has the potential to alleviate liver lipid deposition, and further could be responsible for obesity treatment.

Weak Connection Edges Independent Discriminant of Rapid Spanning Tree Recommendation of Social Network Community

Cao Ke-xing,Li Zhao-xing,Li Xin,Lv Zhi-han4 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.3

Aiming at the problem of low accuracy and high computational complexity of the traditional social network community recommendation algorithm, a rapid spanning tree detection algorithm is proposed to independently discriminate social network community with the weak connected edge, in order to improve the accuracy of community recommendation and reduce the complexity of algorithm. Firstly, according to the characteristics of social network community recommendation, the maximum spanning tree algorithm is proposed, which is based on the edge weight distribution node similarity, to realize the effective detection of social network community. Secondly, for the proposed algorithm having the problems of repeated adding and deleting of weakly connected edges and the waste of computing resources, a rapid spanning tree detection algorithm based on the independent discrimination of weakly connected edge is proposed so as to further improve the calculation efficiency of the algorithm. Lastly, the effectiveness of the proposed algorithm is verified by comparing the experimental results in the standard test database.

Expression profiles of microRNAs in skeletal muscle of sheep by deep sequencing

Zhi-Jin Liu,Cun-Yuan Li,Xiao-Yue Li,Yang Yao,Wei Ni,Xiang-Yu Zhang,Yang Cao,Wureli Hazi,Dawei Wang,Renzhe Quan,Shuting Yu,Yuyu Wu,Songmin Niu,Yulong Cui,Yaseen Khan,Shengwei Hu 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.6

Objective: MicroRNAs are a class of endogenous small regulatory RNAs that regulate cell proliferation, differentiation and apoptosis. Recent studies on miRNAs are mainly focused on mice, human and pig. However, the studies on miRNAs in skeletal muscle of sheep are not comprehensive. Methods: RNA-seq technology was used to perform genomic analysis of miRNAs in prenatal and postnatal skeletal muscle of sheep. Targeted genes were predicted using miRanda software and miRNA-mRNA interactions were verified by quantitative real-time polymerase chain reaction. To further investigate the function of miRNAs, candidate targeted genes were enriched for analysis using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment. Results: The results showed total of 1,086 known miRNAs and 40 new candidate miRNAs were detected in prenatal and postnatal skeletal muscle of sheep. In addition, 345 miRNAs (151 up-regulated, 94 down-regulated) were differentially expressed. Moreover, miRanda software was performed to predict targeted genes of miRNAs, resulting in a total of 2,833 predicted targets, especially miR-381 which targeted multiple muscle-related mRNAs. Furthermore, GO and KEGG pathway analysis confirmed that targeted genes of miRNAs were involved in development of skeletal muscles. Conclusion: This study supplements the miRNA database of sheep, which provides valuable information for further study of the biological function of miRNAs in sheep skeletal muscle.

Expression profiles of circular RNAs in sheep skeletal muscle

Cao, Yang,You, Shuang,Yao, Yang,Liu, Zhi-Jin,Hazi, Wureli,Li, Cun-Yuan,Zhang, Xiang-Yu,Hou, Xiao-Xu,Wei, Jun-Chang,Li, Xiao-Yue,Wang, Da-Wei,Chen, Chuang-Fu,Zhang, Yun-Feng,Ni, Wei,Hu, Sheng-Wei Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.10

Objective: Circular RNAs (circRNAs) are a newfound class of non-coding RNA in animals and plants. Recent studies have revealed that circRNAs play important roles in cell proliferation, differentiation, autophagy and apoptosis during development. However, there are few reports about muscle development-related circRNAs in livestock. Methods: RNA sequencing analysis was employed to identify and annotate circRNAs from longissimus dorsi of sheep. Reverse transcription followed by real-time quantitative (q) polymerase chain reaction (PCR) analysis verified the presence of these circRNAs. Targetscan7.0 and miRanda were used to analyse the interaction of circRNA-microRNA (miRNA). To investigate the function of circRNAs, an experiment was conducted to perform enrichment analysis hosting genes of circRNAs using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathways. Results: About 75.5 million sequences were obtained from RNA libraries of sheep skeletal muscle. These sequences were mapped to 729 genes in the sheep reference genome. We identified 886 circRNAs, including numerous circular intronic RNAs and exonic circRNAs. Reverse transcription PCR (RT-PCR) and DNA sequencing analysis confirmed the presence of several circRNAs. Real-Time RT-PCR analysis exhibited resistance of sheep circRNAs to RNase R digestion. We found that many circRNAs interacted with muscle-specific miRNAs involved in growth and development of muscle, especially circ776. The GO and KEGG enrichment analysis showed that hosting genes of circRNAs was involved in muscle cell development and signaling pathway. Conclusion: The study provides comprehensive expression profiles of circRNAs in sheep skeletal muscle. Our study offers a large number of circRNAs to facilitate a better understanding of their roles in muscle growth. Meanwhile, we suggested that circ776 could be analyzed in future study.

Alternative Splicing of Breast Cancer Associated Gene BRCA1 from Breast Cancer Cell Line

( Li Xia Miao ),( Zhi Jian Cao ),( Chao Shen ),( Chao Jiang Gu ),( Cong Yi Zheng ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.1

Microstructure Evolution During Continuous Cooling in Niobium Microalloyed High Carbon Steels

Zhaodong Li,Qilong Yong,Zhengyan Zhang,Xinjun Sun,Jianchun Cao,Haiquan Qi,Zhi Liao 대한금속·재료학회 2014 METALS AND MATERIALS International Vol.20 No.5

Effects of microalloyed niobium (Nb) on the austenite decomposition behaviors and microstructure evolutionduring continuous cooling in the near eutectoid steels were investigated. Compared to the Nb freesteel, the Nb microalloyed steel was refined with regard to polygonal ferrite grain, pearlite block and colonysizes. This was because its austenite grain size was smaller. The volume fraction of polygonal ferrite transformedwas more in the Nb microalloyed steels, which indicated the eutectoid carbon content exceededthat of pure carbon steel. The spheroidization of pearlite during continuous cooling was enhanced by Nbmicroalloying, mainly due to a higher critical transformation temperature and the finer pearlite structurewith smaller colony size and narrower interlamellar spacing. Hot deformation right above the equilibriumeutectoid temperature accelerated the spheroidization kinetics of pearlite, especially in the Nb microalloyedsteel.

CHRNA5 rs16969968 Polymorphism Association with Risk of Lung Cancer - Evidence from 17,962 Lung Cancer Cases and 77,216 Control Subjects

Xu, Zhi-Wei,Wang, Guan-Nan,Dong, Zhou-Zhou,Li, Tao-Hong,Cao, Chao,Jin, Yu-Hong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15

Background: Genetic studies have shown a possible relationship between the rs16969968 polymorphism in CHRNA5 and the risk of lung cancer. However, the results have been conflicting. Thus we rigorously conducted a meta-analysis to clarify any association. Materials and Methods: A total of 10 case-control studies involving 17,962 lung cancer cases and 77,216 control subjects were analysed. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to measure the strength of the association. Results: We found the CHRNA5 rs16969968 polymorphism to be associated with the risk of lung cancer (AA vs GG: OR=1.60, 95%CI=1.51-1.71). On stratified analysis by smoking status, a statistically significant increased risk was observed in the smoking group (AA vs GG: OR=1.80, 95%CI=1.61-2.01). However, this polymorphism was not associated with lung cancer risk in Asians (AA vs GG: OR=0.95, 95%CI=0.35-2.59), whereas it was linked to increased risk of lung cancer among Caucasians (AA vs GG: OR=1.65, 95%CI=1.55-1.76). Conclusions: Our meta-analysis provided statistical evidence for a strong association between rs16969968 polymorphism and the risk of lung cancer, especially in smokers and Caucasians. Application of this relationship may contribute to identification of individuals at high risk of lung cancer and indicate a chemoprevention target.