Impact of a Glyphosate-Tolerant Soybean Line on the Rhizobacteria, Revealed by Illumina MiSeq

( Gui-hua Lu ),( Yin-ling Zhu ),( Ling-ru Kong ),( Jing Cheng ),( Cheng-yi Tang ),( Xiao-mei Hua ),( Fan-fan Meng ),( Yan-jun Pang ),( Rong-wu Yang ),( Jin-liang Qi ),( Yong-hua Yang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3

The global commercial cultivation of transgenic crops, including glyphosate-tolerant soybean, has increased widely in recent decades with potential impact on the environment. The bulk of previous studies showed different results on the effects of the release of transgenic plants on the soil microbial community, especially rhizosphere bacteria. In this study, comparative analyses of the bacterial communities in the rhizosphere soils and surrounding soils were performed between the glyphosate-tolerant soybean line NZL06-698 (or simply N698), containing a glyphosate-insensitive EPSPS gene, and its control cultivar Mengdou12 (or simply MD12), by a 16S ribosomal RNA gene (16S rDNA) amplicon sequencing-based Illumina MiSeq platform. No statistically significant difference was found in the overall alpha diversity of the rhizosphere bacterial communities, although the species richness and evenness of the bacteria increased in the rhizosphere of N698 compared with that of MD12. Some influence on phylogenetic diversity of the rhizosphere bacterial communities was found between N698 and MD12 by beta diversity analysis based on weighted UniFrac distance. Furthermore, the relative abundances of part rhizosphere bacterial phyla and genera, which included some nitrogen-fixing bacteria, were significantly different between N698 and MD12. Our present results indicate some impact of the glyphosate-tolerant soybean line N698 on the phylogenetic diversity of rhizosphere bacterial communities together with a significant difference in the relative abundances of part rhizosphere bacteria at different classification levels as compared with its control cultivar MD12, when a comparative analysis of surrounding soils between N698 and MD12 was used as a systematic contrast study.

Research Articles : Genetic analysis of the related traits of flowering and silk for hybrid seed production in maize

( Hui Ling Xie ),( Dong Ding ),( ZI Tian Cui ),( Xin Wu ),( Yan Min Hu ),( Zong Hua Liu ),( Yu Ling Li ),( Ji Hua Tang ) 한국유전학회 2010 Genes & Genomics Vol.32 No.1

Differential Impacts on Bacterial Composition and Abundance in Rhizosphere Compartments between Al-Tolerant and Al-Sensitive Soybean Genotypes in Acidic Soil

Wen Zhong-Ling,Yang Min-Kai,Fazal Aliya,Liao Yong-Hui,Cheng Lin-Run,Hua Xiao-Mei,Hu Dong-Qing,Shi Ji-Sen,Yang Rong-Wu,Lu Gui-Hua,Qi Jin-Liang,Zhi Hong,Qian Qiu-Ping,Yang Yong-Hua 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.8

In this study, two soybean genotypes, i.e., aluminum-tolerant Baxi 10 (BX10) and aluminumsensitive Bendi 2 (BD2), were used as plant materials and acidic red soil was used as growth medium. The soil layers from the inside to the outside of the root are: rhizospheric soil after washing (WRH), rhizospheric soil after brushing (BRH) and rhizospheric soil at two sides (SRH), respectively. The rhizosphere bacterial communities were analyzed by high-throughput sequencing of V4 hypervariable regions of 16S rRNA gene amplicons via Illumina MiSeq. The results of alpha diversity analysis showed that the BRH and SRH of BX10 were significantly lower in community richness than that of BD2, while the WRH exhibited no significant difference between BX10 and BD2. Among the three sampling compartments of the same soybean genotype, WRH had the lowest community richness and diversity while showing the highest coverage. Beta diversity analysis results displayed no significant difference for any compartment between the two genotypes, or among the three different sampling compartments for any same soybean genotype. However, the relative abundance of major bacterial taxa, specifically nitrogen-fixing and/or aluminum-tolerant bacteria, was significantly different in the compartments of the BRH and/or SRH at phylum and genus levels, indicating genotype-dependent variations in rhizosphere bacterial communities. Strikingly, as compared with BRH and SRH, the WRH within the same genotype (BX10 or BD2) always had an enrichment effect on rhizosphere bacteria associated with nitrogen fixation

Fotemustine, Teniposide and Dexamethasone in Treating Patients with CNS Lymphoma

Wu, Jing-Jing,Wang, Xin-Hua,Li, Ling,Li, Xin,Zhang, Lei,Sun, Zhen-Chang,Fu, Xiao-Rui,Ma, Wang,Chang, Yu,Zhang, Xu-Dong,Han, Li-Juan,Zhang, Ming-Zhi Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Purpose: We developed and evaluated a regimen including fotemustine, teniposide and dexamethasone (FTD) for treating patients with central nervous system (CNS) lymphoma based on pharmacokinetic properties of individual agents and in combination. Patients and Methods: In a comparison study, 8 patients with primary CNS lymphoma (PCNSL) and 8 with secondary CNS lymphoma (SCNSL) were treated with FTD (comprising fotemustine 100 mg/m2, 1h infusion, day 1; teniposide 60 mg/m2, >0.5 h infusion, on day 2, 3, 4; dexamethasone 40 mg, 1h infusion, on day 1, 2, 3, 4 and 5; and methotrexate 12 mg, cytosine arabinoside 50 mg plus dexamethasone 5 mg intrathecally, on day 2 and 7). Cycles were repeated every 3 weeks. After response assessment, patients received whole brain radiotherapy. Results: Of the 8 PCNSL patients, 4 (50%) achieved CR and 3 (38%) PR, an overall response rate of 88%. Four patients (50%) were in continuing remission at the end of this study after a median follow-up of 30 months (range 10 to 56 months). Of the 8 SCNSL patients the overall response rate was 63% (CR+PR: 38%+25%). All responses were achievable with predictable toxicity mainly reflecting reversible myelosuppression. Conclusion: This study suggests that FTD could be an effective treatment for CNS lymphoma, and is worthy of further evaluation.

Acute kidney injury in hospitalized adults with chronic kidney disease: comparing cROCK, KDIGO, and combined criteria

Ling Sun,Xue Hua Rui,Yu Wu,Lu Xi Zou 대한신장학회 2023 Kidney Research and Clinical Practice Vol.42 No.5

Background: Acute-on-chronic kidney disease (ACKD) increases the risk of progression of chronic kidney disease (CKD). This study aimed to evaluate the ability of a novel criteria of reference change value of the serum creatinine optimized criteria for acute kidney injury in CKD (cROCK) to detect ACKD patients. Methods: This was a retrospective observational study with a 3-year follow-up. All included patients with CKD stage 3 were evaluated using cROCK, Kidney Disease Improving Global Outcomes (KDIGO), and their combined criteria. The renal composite endpoints, major adverse cardiovascular events (MACEs), and all-cause mortality were recorded as clinical outcomes. Results: A total of 812 patients was enrolled. The cROCK criteria detected more ACKD events than did the KDIGO (68.0% vs. 59.5%, p < 0.001). Compared to KDIGO (−) & cROCK (−) group, ACKD patients diagnosed by cROCK had significantly higher hazard ratio [HR] for renal composite endpoints (HR, 3.591; p < 0.001), MACEs (HR, 1.748; p < 0.001), and all-cause mortality (HR, 2.985; p < 0.001). The patients in KDIGO (+) & cROCK (+) group had the lowest survival probability when considering renal composite endpoints, MACEs, and all-cause mortality (all p < 0.001). Furthermore, cROCK resulted in the largest area under the receiver operating characteristic curve (AUC) for predicting renal composite endpoints, and the combined criteria led to the largest AUC for predicting MACEs and allcause mortality. Conclusion: Compared to the KDIGO, the cROCK detected more ACKD events. Combining both cROCK and KDIGO criteria might improve the predictive ability for long-term outcomes in ACKD patients.

PCR-mediated Recombination of the Amplification Products of the Hibiscus tiliaceus Cytosolic Glyceraldehyde-3-phosphate Dehydrogenase Gene

( Ling Hui Wu ),( Tian Tang ),( Ren Chao Zhou ),( Su Hua Shi ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.2

Liner sweep voltammetry electroplating method to synthesize large monocrystalline Cu cones for interconnection

Hua Hu,Ruoxun Zhang,Yunwen Wu,Huiqin Ling,Tao Hang,Ming Li 대한금속·재료학회 2022 ELECTRONIC MATERIALS LETTERS Vol.18 No.1

As the size of interconnect technology in integrated circuits keeps minimizing, the electrical resistivity and signal transmission delay become increasingly serious. The monocrystalline materials can meet the requirements of miniaturization and high-speed interconnection, which is a proper solution of these problems. Hence, we fabricated large monocrystalline Cu cones with (111) orientation using a linear sweep voltammetry electroplating method for the first time. It was found that phosphorus could induce texturization of polycrystals at a certain potential. The grains with uniform orientation grown into single crystals through the oriented attachment growth mechanism. The screw dislocations produced during oriented attachment led to crystal spiral growth, developing into large monocrystalline cones. This work is expected for application in copper interconnect technology in the future.

Giant Diamagnetism in AuFe Nanoparticles

Jun-Hua Wu,Ji Hyun Min,Hong-Ling Liu,Ji Ung Cho,Young Keun Kim IEEE 2009 IEEE transactions on magnetics Vol.45 No.6

<P>Multifunctional Au<SUB>0.5</SUB>Fe<SUB>0.5</SUB> alloy nanoparticles from a polyol process were investigated, showing the alloy nanostructures with the formation of a new phase and twin microstructures. Giant diamagnetism, on the order of -10<SUP>-</SUP> <SUP>2</SUP> emu g<SUP>-1</SUP> Oe<SUP>-1</SUP> , is observed in the zero-field cooled process of the nanoparticles, which is modeled in terms of the spin-orbital interactions tuned by the modified potential energy as a result of the shrinking dimension, peculiar disordered microstructure, and heterogeneous elemental composition.</P>

Magnetic-Optical AuFe Alloy Nanoparticles

Hong Ling Liu,Jun Hua Wu,Ji Hyun Min,Young Keun Kim 한국자기학회 2007 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Smart nanoparticles (NPs) of multiple components offer exciting opportunities in fundamental studies and highly multidisciplinary nanotechnology that has rapidly grown with tremendous applications in many areas including medicine, life science, materials science, environment, electronics, and energy. Metallic alloy NPs have been extensively investigated for various purposes such as magnetic recording, drug delivery, bioseparation, medical diagnosis, and catalysis, particularly magnetic and optical properties of materials are widely pursued. Since fusion of the magnetic and optical elements in one single entity promises multifunctionality and potential applications, so a great deal of effort is instilled to prepare such nanoparticles containing Au and Fe. We have investigated a variety of functional nanoparticles via an efficient, scalable and non-toxic synthesis approach [1]. In this work, we report the preparation and characterization of multifunctional magnetic-optical AuFe alloy nanoparticles, integrating the optical functionality of Au composition and the magnetic properties of iron. The nanoparticles were synthesized by a modified polyol process using iron acetylacetonate (Fe(acac)3) and gold acetate (Au(ac)3) in the presence of surfactants at high temperature[1].Three compositions of AuFe nanoparticles, Au0.25Fe0.75, Au0.5Fe0.5, and Au0.75Fe0.25, were formed and investigated on the structure and properties as a function of composition by TEM/HRTEM, XRD, UV-vis, XPS and VSM/SQUID. It is found that the fusion of the two materials into one nanostructure entity retains the optical and magnetic properties of the individual components (Figs.1,2). The XRD and TEM analysis confirms the formation of the alloy nanostructure and provides the detailed structural arrangements, with a narrow distribution of particle sizes in the range of 5~10 ㎚ (Figs.34). X-ray photoelectron spectroscopy reveals the binding energies of Au and Fe in the state of the AuFe alloy nanoparticles. The magnetic characterization shows the superparamagnetic or soft ferromagnetic behavior (Fig.1) of the nanoparticles at room temperature, while the thermal response to different external fields was obtained from the M~T measurements in the FC and ZFC modes, respectively. The UV-vis spectra display the variation of the absorption bands at ~560㎚ with composition (Fig.2), which is characteristic of nanostructured gold. These AuFe nanoparticles are water soluble after thiolation.

A Characteristic Analysis and Countermeasure Study of the Hedging of Listed Companies in China Stock Markets

Guo-Hua WU,Xiao-Ling JIANG,Su-Ya DENG 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.10

Due to COVID-19, the risk of price volatility in commodity and equity markets increases. The research and application of hedging is the most effective way to reduce the market risk. Hedging is a risk management strategy employed to offset losses in investments by taking an opposite position in a related asset. We use K-means and hierarchical clustering methods to cluster companies and futures products respectively, and analyze the relationship between the number of hedging firms, regional distribution, nature of firms, capital distribution, company size, profitability, number of local Futures Commission Merchants (FCMs), regional location, and listing time. The study shows that listed companies with large scale and good profitability invest more money in hedging, while state-owned enterprises’ participation in hedging is more likely to be affected by the company size and the number of local futures commission merchants, and private enterprises are more likely to be affected by the company profitability and the regional location. Listed companies are more willing to choose long-listed and mature futures products for hedging. We also provide policy advice based on our conclusion. So far, there is no study on the characteristics of hedging. This paper fills the gap. The results provide a basis and guidance for people’s investment and risk management. Using clustering analysis in hedging study is another innovation of this paper.