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Impact of a Glyphosate-Tolerant Soybean Line on the Rhizobacteria, Revealed by Illumina MiSeq
( Gui-hua Lu ),( Yin-ling Zhu ),( Ling-ru Kong ),( Jing Cheng ),( Cheng-yi Tang ),( Xiao-mei Hua ),( Fan-fan Meng ),( Yan-jun Pang ),( Rong-wu Yang ),( Jin-liang Qi ),( Yong-hua Yang ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3
The global commercial cultivation of transgenic crops, including glyphosate-tolerant soybean, has increased widely in recent decades with potential impact on the environment. The bulk of previous studies showed different results on the effects of the release of transgenic plants on the soil microbial community, especially rhizosphere bacteria. In this study, comparative analyses of the bacterial communities in the rhizosphere soils and surrounding soils were performed between the glyphosate-tolerant soybean line NZL06-698 (or simply N698), containing a glyphosate-insensitive EPSPS gene, and its control cultivar Mengdou12 (or simply MD12), by a 16S ribosomal RNA gene (16S rDNA) amplicon sequencing-based Illumina MiSeq platform. No statistically significant difference was found in the overall alpha diversity of the rhizosphere bacterial communities, although the species richness and evenness of the bacteria increased in the rhizosphere of N698 compared with that of MD12. Some influence on phylogenetic diversity of the rhizosphere bacterial communities was found between N698 and MD12 by beta diversity analysis based on weighted UniFrac distance. Furthermore, the relative abundances of part rhizosphere bacterial phyla and genera, which included some nitrogen-fixing bacteria, were significantly different between N698 and MD12. Our present results indicate some impact of the glyphosate-tolerant soybean line N698 on the phylogenetic diversity of rhizosphere bacterial communities together with a significant difference in the relative abundances of part rhizosphere bacteria at different classification levels as compared with its control cultivar MD12, when a comparative analysis of surrounding soils between N698 and MD12 was used as a systematic contrast study.
Cheng-Hua, Jin 원광대학교 법학연구소 2010 圓光法學 Vol.26 No.1
In recent years, there has been a phenomenon which happens in Jiaodong Peninsula and Pearl River Delta of china that some foreign-funded enterprises disappear overnight. The abnormal withdrawal of the foreign-funded enterprise has occurred frequently since the breaking out of American economic crisis. And this tendency is increasing. This not only brings serious economic losses to our country, but also has a negative effect on economic and trade exchanges and social stability. The phenomenon has been a matter of great concern ofthe central and local government departments and interested parties. At the same time, the government has issued some department rules and remedies. However, there are still many shortcomings in analysis and legal measures for the abnormal withdrawal of the foreign-funded enterprise now. So it is necessary to deeply analyze the phenomenon and the juristical puzzledom in the legal perspective. What's more, the effective remedy mechanism should be put forward.
Cheng-hua Shi,Cheng-yong Cao,Ming-feng Lei 대한토목학회 2017 KSCE Journal of Civil Engineering Vol.21 No.5
The ground movement induced by the construction of shield tunnels affects the safety of nearby underground pipes and aboveground structures. Therefore, the reliable prediction of ground movement is important. In this paper, a model describing the interaction between soil and an EPB shield used in tunnels is presented, based on the classical elastic theory of Mindlin. In this model, the changing location of the working shield is considered. The equations describing the ground deformation around the tunnel caused by the additional force on the shield working surface and the frictional force between the outer surface of the shield and the surrounding soil are derived. The ground deformation caused by ground loss due to shield tunnel construction is derived using stochastic medium theory. Finally, this model is applied to two running tunnels, and the sensitivity of the ground deformation to the calculation parameters is discussed.
Cheng-yong Cao,Cheng-hua Shi,Ming-feng Lei,Li-min Peng,Ruxue Bai 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.8
Hongmian Road goes under Jihe highway by the way of tunnel in Shenzhen City, Guangdong Province, China. The tunnel hassome special characteristics such as shallow depth, large span, soft soil, busy traffic, and long distance under-crossing the operatinghighway, thus there is great difficulty and risk during its construction. A series of in-situ tests were conducted to reveal deformationcharacteristics during tunneling beneath the existing highway by the double-side-drift method (DSDM). The results show that initialconstruction method and measures can't meet the safety requirement of tunnel construction, and the level of risk is extremely high, sothe construction method need to be timely adjusted. By comprehensive analysis, the double-side-drift optimum method (DSDOM) isput forward, on the basis of which a series of optimum measures also have been put forward, such as reasonable constructionmethods, the suitable length of bench and excavation, soil reinforcement measures on tunnel face, optimizing construction process,closing primary support in time and speeding up the construction of secondary lining. Then the feasibility of this optimizedconstruction method and measures are verified by the numerical simulations. Finally, from feedbacks of monitoring results, theoptimum method and measures have been proved to be reasonable and feasible, and they can guarantee a successful pass beneath theoperating highway.
Glycerol steam reforming over Ni/γ-Al2O3 catalysts modified by metal oxides
Cheng-Hua Xu,Zun-Yu Huang,Chuan-Qi Liu,Hui-Wen Xiao,Jun Chen,Yong-Xiang Zhang,Ya-Cong Lei 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.3
The metal oxides modified Ni/γ-Al2O3 catalysts for glycerol steam reforming were prepared by impregnation. Characterization results of fresh catalysts indicated that the molybdates modification abated the acidity and the stronger metal-support interaction of Ni/γ-Al2O3 catalysts, leading to a stable catalytic activity. Especially, NiMoLa-CaMg/γ-Al2O3 (NiMoLa/CMA) catalyst exhibited no deactivation along with glycerol complete conversion to stable gaseous products containing 69% H2, 20% CO and 10% CO2 during time-on-stream of 42 h. TPO of spent Ni/γ-Al2O3catalysts modified by different components showed that the carbon deposit on acidic sites and NiAl2O4 species led to catalysts deactivation. A lower reforming temperature and a higher LHSV and glycerol content were helpful to the production of syngas from GSR over NiMoLa/CMA; the reverse conditions would improve the formation of H2.
Design and Evaluation of a Scalding Animal Model by the Boiling Water Method
Cheng Hua,Lele Lyu,류현석,So Young Park,임남규,ABUEVA CELINE,Phil-Sang Chung 대한의학레이저학회 2020 MEDICAL LASERS Vol.9 No.1
Background and ObjectivesFor experiments on simulated burn, the preparation of an animal model is a very important step. The purpose of the current experiment is to design a simple and controllable method for the preparation of third-degree scald in a mouse model using the boiling water method. Materials and MethodsA total of 18 Swiss mice were used. After the anesthetization, the mice were scalded by boiling water (100°C) using a mold with a 1 cm2 circle area on the dorsum at contact times of 3s, 5s, and 8s. After confirming that 8 seconds of scald can cause a third-degree scald, the skin samples were collected at day 2, 4, 6, 8, 10, and 12, and analyzed by histopathological examinations. The wound retraction index (WRI) was also measured. ResultsThird-degree scald involving full-thickness skin was observed in the 8-second scald group, while a 3-second scald caused a superficial second-degree scald and a 5-second scald caused a deep second-degree scald. After third-degree scald, the burn wound continued to contract until day 14. ConclusionThe scalding model of mice can be successfully established by the boiling water method. This method is easy to operate, it has a low cost, and it can control the scald depth by controlling the scald time. This is adequate to study skin thermal injury in the future. The scald model established by this method can last for 14 days.
Structure and bioactivity of triterpenoids from the stems of Schisandra sphenanthera
Cheng-Qin Liang,Rong-Hua Luo,Ju-Ming Yan,Yan Li,Xiao-Nian Li,Yi-Ming Shi,Shan-Zhai Shang,Zhong-Hua Gao,Liu-Meng Yang,Yong-Tang Zheng,Wei-Lie Xiao,Hong-Bin Zhang,Han Dong Sun 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.2
Two new triterpenoids, schisphendilactoneA and B (1 and 2), together with three known triterpenoids,were isolated from the stems of Schisandra sphenanthera. Their structures were elucidated by spectroscopic methods,and the absolute configuration of 1 was determined bysingle-crystal X-ray diffraction. Compound 2 showedmoderate inhibitory activity against SW480 cancer cellline, and compound 5 exhibited promising anti-HIV-1activity with EC50 value of 0.52 lg ml-1 and therapeuticindex value of 117.12.