Therapeutic Effect of Dioctahedral Smectite on Diarrhea Caused by E. coli and Salmonella in Calves

Hu Jang Lee1,3*, Chun-Nam Cha, Yeo-Eun Lee, Hyunju Choi, Joon-Seob Shin, Suk Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.2

The objectives of the current study were to evaluate the therapeutic effect of dioctahedral smectite (smectite) against calf diarrhea caused by pathogenic E. coli and/or Salmonella typhimurium. In this study, 20 calves (aged 2~3 months) with diarrhea were used for evaluation of the efficacy of smectite on calf diarrhea with 20% smectite suspension in PBS. Calves received 10 ml smectite suspension three times per day after feeding, and fecal samples were collected at the gate of treatment and on the first, second, third, fourth, and fifth day after administration. On the fifth day after treatment with smectite suspension, the diarrhea index showed a significant decrease in the treated group, compared to the control group (P<0.001). The number of pathogenic E. coli in feces of the treated group was significantly decreased, compared to each control group from the second day after treatment (P<0.001), and that of Salmonella typhimurium was significantly decreased from the first day after treatment (P<0.05). According to the results of the current study, 20% smectite suspension had a therapeutic effect on diarrhea caused by E. coli and/or Salmonella in calves.

우모 분해균의 분리 및 특성에 관한 연구

강호조,이후장,정태성,김도경,어용준,김재황 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.3

Feather waste, generated in large quantities as a byproduct of commercial poultry processing, is almost pure keratin, which is not easily degradable by common proteolytic enzymes. Several feather-degrading bacterial isolates were isolated from feather waste and soil. These isolates were able to degrade chicken feather, when grown on basal medium containing 1.0% native feather as a source of energy, carbon, and nitrogen. The isolates were characterized according to morphological characteristics and biochemical tests, and Vitek and API kit system. belonging to the Acinetobacter calcoaceticus complex, Bacillus thuringiensis, Bacillus circulans, Bacillus cereus. It was observed that aerobic growth by the isolate on single whole chicken feather, resulted in complete degradation of the keratin protein after 5-6 days of incubation at 37℃. The production of protein by the organism was decreased with cultivation time, with the maximum activity coinciding with the start of the stationary phase. The kerationolytic activity increased with cultivation time.

Interfacial nanostructure induced spin-reorientation transition in Ni/Fe/Ni/W(110)

Lee, J.-S.,Sadowski, J. T.,Jang, H.,Park, J.-H.,Kim, J.-Y.,Hu, J.,Wu, R.,Kao, C.-C. American Physical Society 2011 Physical review. B, Condensed matter and materials Vol.83 No.14

Awareness of Asbestos and Action Plans for Its Exposure can Help Lives Exposed to Asbestos

Lee, Hu-Jang,Park, Eun-Kee,Wilson, Donald,Tutkun, Engin,Oak, Chulho Occupational Safety and Health Research Institute 2013 Safety and health at work Vol.4 No.2

Despite the fact that asbestos is a known carcinogen to humans, it is still used in industrialized countries, especially Asian countries. The global incidence of asbestos-related diseases (ARDs) due to the past use of asbestos, continues to increase, although many countries have adopted a total ban on asbestos use. The implementation of effective strategies to eliminate ARDs is therefore an important challenge in Asia, where asbestos is still mined and consumed. Collaborative efforts and strategies at the local and international levels are vital, in the pursuit toward the elimination of ARDs in this region.

Proteomic analyses reveal that ginsenoside Rg3(S) partially reverses cellular senescence in human dermal fibroblasts by inducing peroxiredoxin

Jang, Ik-Soon,Jo, Eunbi,Park, Soo Jung,Baek, Su Jeong,Hwang, In-Hu,Kang, Hyun Mi,Lee, Je-Ho,Kwon, Joseph,Son, Junik,Kwon, Ho Jeong,Choi, Jong-Soon The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.1

Background: The cellular senescence of primary cultured cells is an irreversible process characterized by growth arrest. Restoration of senescence by ginsenosides has not been explored so far. Rg3(S) treatment markedly decreased senescence-associated β-galactosidase activity and intracellular reactive oxygen species levels in senescent human dermal fibroblasts (HDFs). However, the underlying mechanism of this effect of Rg3(S) on the senescent HDFs remains unknown. Methods: We performed a label-free quantitative proteomics to identify the altered proteins in Rg3(S)-treated senescent HDFs. Upregulated proteins induced by Rg3(S) were validated by real-time polymerase chain reaction and immunoblot analyses. Results: Finally, 157 human proteins were identified, and variable peroxiredoxin (PRDX) isotypes were highly implicated by network analyses. Among them, the mitochondrial PRDX3 was transcriptionally and translationally increased in response to Rg3(S) treatment in senescent HDFs in a time-dependent manner. Conclusion: Our proteomic approach provides insights into the partial reversing effect of Rg3 on senescent HDFs through induction of antioxidant enzymes, particularly PRDX3.

Effect of feeding the combination with Lactobacillus plantarum and Bacillus subtilis on fecal microflora and diarrhea incidence of Korean native calves

Lee, Yeo-Eun,Kang, In-Jin,Yu, Eun-Ah,Kim, Suk,Lee, Hu-Jang The Korean Society of Veterinary Service 2012 韓國家畜衛生學會誌 Vol.35 No.4

This study was investigated the effects of feeding the combination with Lactobacillus plantarum and Bacillus subtilis on the diarrhea incidence and fecal microflora of weaned calves. A total of 12 newly weaned calves were allocated to two dietary treatments in a randomized design based on body weight. The dietary treatments included a commercial basal diet supplemented with: 1) no microbial inoculants (Control); 2) a mixture of Lactobacillus plantarum and Bacillus subtilis (LB). Calves were fed diets for a 4-week period. At the end of the experiment, the counts of fecal lactic acid bacteria and Enterobacteriaceae in LB were significantly improved compared to control (P<0.05). Over the 4-week period, fecal scores and duration of diarrhea in LB were significantly decreased compared with those in control (P<0.05). The present results suggest that LB is a potential feed additive which could be used for the balance of intestinal microflora and the prevention of diarrhea in Korean native calves.

Effects of mixed formulation of tamoxifen and blue honeysuckle on the pharmacokinetics profiles of tamoxifen after single oral administration

Hu, Jin-Ryul,Jang, Tae-Woo,Kang, Su-Jin,Ku, Sae-Kwang,Choi, Seong-Hun,Lee, Young-Joon The Society of Korean Medicine 2019 대한한의학회지 Vol.40 No.4

Objectives: Here, we investigated the effects of concentrated and lyophilized powders Blue honeysuckle (BH) on the PK of tamoxifen, to establish the pharmacokinetics (PK) profiles as one of essential process in new drug development. Methods: After single oral treatment of 0.4 mg/ml of tamoxifen or tamoxifen 0.4 with BH 40, 20 and 10 mg/ml, the plasma were collected at 0.5 hr before administration, 0.5, 1, 2, 3, 4, 6, 8 and 24 hr after end of single or mixed formula treatment. Plasma concentrations of tamoxifen were analyzed using LC-MS/MS methods. Tmax, Cmax, AUC, t1/2 and MRTinf were analyzed using noncompartmental PK data analyzer programs. Results: Tamoxifen and BH 40 mg/ml did not induce any significant change on the plasma tamoxifen concentrations, while significant decreases were observed in tamoxifen and BH 10 mg/ml from 2 to 8 hr as compared with tamoxifen only, respectively. Furthermore, significant increases of Tmax in tamoxifen and BH 40 mg/ml, significant decreases of Cmax in tamoxifen and BH 20 mg/ml, significant decreases of AUC0-t, AUC0-inf and MRTinf in tamoxifen and BH 10 mg/ml were demonstrated as compared with tamoxifen only. Conclusion: Taken together, tamoxifen and BH 10 mg/ml induced significant decrease of the oral bioavailability of tamoxifen, while tamoxifen and BH 40 or 20 mg/ml did not critically influenced, suggesting formulated BH concentration-independencies. It, therefore, seems to be needed that pharmacokinetic study after repeated administration should be tested to conclude the effects of BH on the pharmacokinetics of tamoxifen.

청정지역과 도시지역의 오존농도 특성 연구

장광미,강창희,서명석,허철구,박경윤,이호근,김영준 濟州大學校 基礎科學硏究所 1996 基礎科學硏究 Vol.9 No.2

A study has been performed on the characteristics of rural and urban surface ozone concentration for the period of March 1992 to February 1993. The monitoring station of rural ozone is located at Kosan, Cheju and other urban monitoring stations are located at Seoul, Pusan and Kwangju. Rural's and urban's ozone data exhibit a distinct features in many ways. First, annual mean of rural ozone concentration is very high(42 ppbv) but urban's are very low(10~15 ppbv). Second, rural ozone data shows a seasonal variation with it's maximum in spring, and minimum in summer, but urban's show a seasonal variation with it's maximum in spring, and minimum in winter. Third, diurnal variation of rural data is very small but that of urban's are very large. Fourth, monthly mean of daily minimum of rural data is not low and shows a large seasonal variation, but those of urban's data are extremely low(<3 ppbv) and have no seasonal variations.

165 rDNA PCR을 이용한 양수내 세균 검출

이성현,장숙진,최상준,김영숙,한호림,문대수,박영진 朝鮮大學校 附設 醫學硏究所 2004 The Medical Journal of Chosun University Vol.29 No.1

Background and Objectives : Microbial invasion of the amniotic cavity is a significant clinical problem in obstetrics leading to infectious morbidity of both the mother and the newborn. So rapid and reliable test to detect bacteria in amniotic fluid is urgently needed. The purpose of this investigation was to determine the feasibility of using the 16S rDNA PCR to detect bacteria in amniotic fluid. Material and Method : Amniotic fluid samples from 15 pregnant women with hydramnios, preterm labor, or old age pregnancy were analysed with universal bacterial PCR. We used universal primers which amplify a DNA fragment of 16s rDNA from all known bacteria and sequenced the positive samples to identify the bacterial species, In some cases, routine bacterial culture, WBC, and CRP were studied also. Result : Three of 15 pregnant women were positive in the PCR. DNA sequencing of the PCR product detected Staphylococcus epidermidis in one case, and Streptococcus anginosus in two cases. Conclusion : The broad-spectrum bacterial 16S rDNA PCR assay, in properly controlled conditions, may provide a fast and reliable means for the detection of bacteria in amniotic fluid. 배경 및 목적 : 양수강의 미생물 침습은 산모와 신생아 양자에게 감염성 질환을 야기할 수 있어 산과학에 있어 중요한 임상적 문제이다. 따라서 양수에서 세균을 검출할 수 있는 신속하고 믿을 만한 검사가 절실히 필요하다. 이 연구의 목적은 16S rDNA PCR을 이용하여 양수에서 세균을 검출할 수 있는지 그 실행가능성을 검사하는데 있다. 대상 및 방법 : 양수과다증이나 조기 분만, 노산 등이 있는 임산부 15인의 양수 검체를 16S rDNA PCR로 분석하였다. 모든 알려진 세균의 16S rDNA 분절을 중폭할 수 있는 보편적인 시동물질을 사용하여 증폭하였고, 양성 검체를 염기순서분석하여 세균 균종을 동정하였다. 일부 환자들에서는 세균 배양검사와 WBC, CRP 검사도 함께 시행하였다. 결과 : 15명중 3명의 임산부가 PCR에 양성이었고 PCR 산물을 DNA 염기순서분석한 결과 한 환자에서 Staphylococcus edidermidis가 다른 두 환자에서 Streptococcus anginosus가 검출되었다. 결론 : 광범위 세균 16S rDNA PCR 분석은 적절히 관리된 조건 하에서 양수내 세균 검출을 위한 신속하고 믿을 만한 방법을 제공해 줄 수도 있다고 생각되었다.

EMS-induced Mutagenesis for C18 Unsaturated Fatty Acids in Rapeseed (Brassica napus L.)

Lee, Yong-Hwa,Kim, Kwang-Soo,Jang, Young-Seok,Choi, In-Hu 韓國作物學會 2014 한국작물학회지 Vol.59 No.2

Rapeseed (Brassica napus L.) oil with high oleic acid content is of great interest for both food and non-food uses. The ‘Tamla’ variety, characterized by oleic acid content of approximately 69%, was treated with 1% ethyl methane sulfonate (EMS) to induce mutations in the fatty acid biosynthesis pathway. M1 plants were selfed and subsequent generations (M2, M3, and M4 mutants) were analyzed to identify mutants having increased levels of oleic acid. M2 mutants showed oleic acid content ranging from 13.5% to 76.9% with some mutants (TR-458 and TR-544) having up to 74.7% and 76.9% oleic acid, which was an increase of nearly 5% and 7%, respectively, compared to untreated cv ‘Tamla’. We selected two M3 mutants with >75% oleic acid content. One mutant (TR-458-2) had increased oleic acid (75.9%) and decreased linoleic acid (12.5%) and linolenic acid (4.4%) contents. The other (TR-544-1) showed increased oleic acid content (75.7%) and decreased linoleic acid (13.5%) and linolenic acid (3.3%) contents. The accumulation or reduction of oleic acid content in the selected M4 mutants was also accompanied by a simultaneous decrease or increase in linoleic and linolenic acid contents. The high-oleic lines could be utilized further in breeding programs for improvement of rapeseed oil quality.