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Zhang, Yong-Guang,Liu, Qing,Wang, Hong-Fei,Zhang, Dao-Feng,Zhang, Yuan-Ming,Park, Dong-Jin,Kim, Chang-Jin,Li, Wen-Jun International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.6
<P>A facultatively alkaliphilic actinomycete strain, designated EGI 80088<SUP>T</SUP>, was isolated from a saline-alkali soil sample from Xinjiang province, north-west China, and subjected to a polyphasic taxonomic characterization. Strain EGI 80088<SUP>T</SUP> formed fragmented aerial hyphae and short spore chains, and rod-like spores aggregated at maturity. Whole-cell hydrolysates of the isolate contained <SMALL>ll</SMALL>-diaminopimelic acid as the diagnostic diamino acid, and glucosamine, mannose, galactose, glucose and rhamnose as the marker sugars. The major fatty acids identified (>5 %) were anteiso-C<SUB>15 : 0</SUB>, iso-C<SUB>15 : 0</SUB>, summed feature 4 (iso-C<SUB>17 : 1</SUB>I/anteiso-C<SUB>17 : 1</SUB>B), iso-C<SUB>16 : 0</SUB> and anteiso-C<SUB>17 : 0</SUB>. The predominant menaquinone was MK-9(H<SUB>4</SUB>). The G+C content of the genomic DNA of strain EGI 80088<SUP>T</SUP> was 70.6 mol%. EGI 80088<SUP>T</SUP> showed the highest 16S rRNA gene sequence similarity to its closest phylogenetic neighbour <I>Haloactinopolyspora alba</I> YIM 93246<SUP>T</SUP> (98.5 %). The DNA–DNA relatedness value of the strain EGI 80088<SUP>T</SUP> and <I>H. alba</I> YIM 93246<SUP>T</SUP> was 59.3±5.2 %. On the basis of morphological, chemotaxonomic and phylogenetic characteristics and DNA–DNA hybridization data, strain EGI 80088<SUP>T</SUP> represents a novel species of the genus <I>Haloactinopolyspora</I>, for which the name <I>Haloactinopolyspora alkaliphila</I> sp. nov. (type strain EGI 80088<SUP>T</SUP> = BCRC 16946<SUP>T</SUP> = JCM 19128<SUP>T</SUP>) is proposed. The description of the genus <I>Haloactinopolyspora</I> has also been emended.</P>
Anomalous magnetization jumps in granular Pb superconducting films
Zhang An-Lei,Jiang Wan-Yan,Chen Xing-Hong,Zhang Xiao-Ke,Lu Wen-Lai,Chen Fei,Feng Zhen-Jie,Cao Shi-Xun,Zhang Jin-Cang,Ge Jun-Yi 한국물리학회 2022 Current Applied Physics Vol.35 No.-
In granular superconductors, the grain boundaries are closely related to the vortex dynamics and the macroscopic superconducting properties. In our research, Pb films with different grain sizes were prepared by tuning the substrate temperature. With the grain size decreasing, Pb films are prone to feature the anomalous magnetization jumps in the M − T curves, while in the M − H curves flux avalanche happens. Both phenomena appear in the same region of the H − T phase diagram and thus are considered to have the same origin. The further theoretical analysis shows that with grain size decreasing the pinning mechanism evolves from a mixed δTc and δl pinning to the δl pinning mechanism. The results shed light on the study of pinning mechanism for granular superconductors and is beneficial to the potential application of manipulating vortex pinning by regulation of intrinsic defects.
Chemical Synthesis and Cloning of Panax ginseng Peptide Gene
Zhang, Hong-Ying,Chen, Dong-Song,Zhang, Jin The Korean Society of Ginseng 1990 Journal of Ginseng Research Vol.14 No.2
The sequence of ginseng peptide gene was designed and synthesized by the solid phase phosphoramidiate method. Synthetic segments were isolated, pllrified and joined to the plasmid pUC19. E.icherichiu coli JM101 cells were transformed with above hybrid plasmids. AmpiciIBin resistant transformants were screened and identified by in situ colony hybridization and Southern blot techniques. Finally the gene sequencing was done by the Sanger dideoxy method using primer extension.
Plasma Membrane as the Target Site of Cholic Acid Analogs
Tian Hong Zhang,Zhen Qing Zhang,Chun Guang Liu,Jin Xiu Ruan 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7
Although the mechanism is unknown, Calculus Bovis and its active components, cholic acid analogs (CAAs), have been used in China to treat a wide range of diseases. Based on the previous finding that the potency of CAA is strongly dependent on the intrinsic surface activity, this paper aimed to investigate the role of the plasma membrane in the pharmacological activity of CAAs. First, CAAs (0.1 mM) caused a surface activity-dependent depression on ATPase activity in the cell membrane extract, but it had no effects on other cellular extracts, suggesting an indispensable role of the membrane environment for pharmacological activity. Second, CAAs lowered the membrane fluidity of cultured Caco-2 cells with the same rank-order of potency sequence. Third, the hypothesis that any functional protein located on the membrane is influenced by changes in cellular membrane fluidity was supported by: ileal contraction that was induced by acetylcholine and mediated by the muscarinic receptor (M-receptor) or the relaxation induced by adrenaline and mediated by the β-adrenergic receptor (β-receptor) was inhibited by CAAs. They also had similar rank-order of potency and the effects on the plasma membrane. Collectively, the plasma membrane may be a target for the CAAs to exert the multiple pharmacological effects which are mediated by the alteration of the membrane mobility and the function of integral membrane proteins.
Guang-jin Zhu,Hai-yan Tang,Peng-hui Qing,Hong-ling Zhang,Xi-chuan Cheng,Zai-hua Cai,Hong-bin Xu,Yi Zhang 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.5
An ion-imprinted polymer (IIP) with monophosphonic groups was prepared by thermal copolymerization. Bis(2-methacryloxyethyl) phosphate (BMAOP) was used as functional monomer to react with Fe3+ in dimethyl sulfoxide (DMSO). Ethylene glycol dimethacrylate (EGDMA) was used as cross-linker during polymerization process. A suitable molar ratio of BMAOP to Fe3+ was investigated by UV/Vis/NIR Spectrometer and ICP-OES. The obtained results showed that the monophosphonic groups could be selectively combined with Fe3+ in solutions containing other coexisting ions, and the selectivity could be further enhanced by ion-imprinted process. The prepared IIP was used for removing trace Fe3+ from high concentration basic chromium sulfate solutions. After adsorption process, the concentration of Fe3+ could be reduced from 4.486mg L1 to 0.171mg L1, which was much lower than the concentration in the solution treated by non-imprinted polymer (NIP). Moreover, the IIP exhibited excellent recyclability after six adsorption-desorption cycles.