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      • KCI등재

        각종 EIA시약을 이용한 C형간염항체 검출에 관한 연구

        오영철,최범열,배형준,김기홍,김상인 大韓輸血學會 1992 大韓輸血學會誌 Vol.3 No.1

        Several kinds of anti-HCV EIA reagents are available and the selection of most appropriate reagent is very important for the screening of blood donors. The authors compared Ortho first generation kit based on C 100-3 protein, Organon Taknika second generation kit based on C 100-3, some none-structural and one structural protein and Lucky made kit based on two none-structural protein and one structural protein. Study subjects consist of 637 random blood donor samples and 180 positive blood donors screened by first generation reagent. The results are as follows. In 637 random samples, the positive results by screening EIA were 0.63%(4 cases) by Ortho first generation kit, 0.63%(4cases) by Organon Teknika 2nd generation kit and 0.94%(6cases) by Lucky 2nd generation kit. HCV antibody screening is very useful test for the detection of blood donors, post-transfusion hepatitis and various liverdisease but sensitivity and specificity are relatively low. So futher evaluation and development of reagent for detection of hepatitic C virus infection by enzyme immunoassay.

      • KCI등재SCOPUS
      • KCI등재

        Expression of ssrA in non-pathogen-induced adaptation in the oral cavity through signal exchange with oral pathogens

        Sung-Ryoul Kim,Jae-Woo Kwak,Sung-Ka Lee,Seung-Gon Jung,Man-Seung Han,Bang-Sin Kim,Min-Suk Kook,Hee-Kyun Oh,Hong-Ju Park 대한구강악안면외과학회 2012 대한구강악안면외과학회지 Vol.38 No.1

        Introduction: This study was conducted to evaluate ssrA expression resulting from adaptation of Escherichia coli (E. coli) to oral pathogens through signal exchange. Materials and Methods: Human cell lines Hep2 and HT29, wild-type E. coli (WT K-12), ssrA knock-out E. coli (Δ K-12), and Scleropages aureus (S. aureus) were used. A single culture consisting of Hep2, HT29, WT K-12, and Δ K-12, and mixed cultures consisting of Hep2 and WT K-12, Hep2 and Δ K-12, WT K-12 and S. aureus, Δ K-12 and S. aureus, and Hep2, WT K-12, and S. aureus were prepared. For HT29, a mixed culture was prepared with WT K-12 and with WT K-12 and S. aureus. Total RNA was extracted from each culture with the resulting expression of ssrA, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and p53 was evaluated by Reverse transcription polymerase chain reaction (RT-PCR). Results: The expression of ssrA in a single culture of WT K-12 was lower than that observed in the mixed culture of WT K-12 with S. aureus. Greater ssrA expression was observed in the mixed culture of WT K-12 with Hep2 than in the single culture of WT K-12. The expression of NF-κB was higher in the mixed culture of Hep2 with Δ K-12 than that in the mixed culture of Hep2 with WT K-12, and was lowest in the single culture of Hep2. The expression of ssrA was higher in the mixed culture of WT K-12 with Hep2 and S. aureus than in the mixed culture of WT K-12 with Hep2. Conclusion: These results suggest that ssrA plays an important role in the mechanism of E. coli adaptation to a new environment.

      • A Study on the Long-term Cooling Effect of Hydride Reorientation in Unirradiated Zircaloy-4 Cladding (3, 6 & 12 Months)

        Dae-Ho Kim,Hong-Ryoul Oh,Dong-Hak Kook 한국방사성폐기물학회 2023 한국방사성폐기물학회 학술논문요약집 Vol.21 No.1

        The hydride reorientation (HR) of the post-irradiated nuclear fuel cladding after use affects the integrity of the spent nuclear fuel. During the dry storage process, which is an intermediate storage method, it was found that the hydride in the circumferential direction is rearranged into radial hydride, and this is believed to be due to factors such as hoop stress, peak temperature, accumulated hydrogen concentration, and cooling rate during the storage period. f(HR) = f(Tmax) + f(σH) + f(CH) + f(△T) + f(10Cy) + f(cooling rate) + ...... To simulate long-term dry storage of spent nuclear fuel, the hydride reorientation behavior was evaluated using unirradiated Zircaloy-4 (CWSRA) cladding with hydrogen charged under various hoop stresses (70, 80, 90, and 110 MPa) at long-term cooling periods (3, 6, and 12 months). Test results showed that as the cooling time increased, the sample with 90 MPa hoop stress at a maximum temperature of 400°C approached the ductility recommendation limit of 2%. In a 90 MPa hoop stress specimen with 3 months cooling period at peak temperature of 400°C, the offset strain was 4.24% at room temperature RCT, while it showed the result of 2.86% for the cooling period of 12 months. On the other hand, the specimen with hoop stress of 110 MPa and cooling period of 12 months showed result of 1.4%. The test results need to take into account errors in hydrogen charging and hydrogen analysis, and it is necessary to consider reproducibility through repeated tests. These results indicate the need for continued attention to the evaluation of the effects of hydride reorientation due to long-term cooling in the context of the integrity of spent fuel.

      • SCOPUSKCI등재

        국내 트리티케일 품종의 품질 및 이화학적 특성

        오선민(Seon-Min Oh),최유찬(Yu-Chan Choi),곽지은(Jieun Kwak),박현진(Hyun-Jin Park),이정희(Jeong-Heui Lee),이점식(Jeom-Sig Lee),박명렬(Myoung Ryoul Park),김홍식(Hong-Sik Kim),김현주(Hyun-Joo Kim),오유근(You-Geun Oh) 한국식품과학회 2022 한국식품과학회지 Vol.54 No.6

        본 연구에서는 국내산 트리티케일 3품종(신영, 조성, 신조성)의 이화학적 특성을 조사하고 이를 국내 품종의 밀(백강, 새금강) 및 호밀(씨드그린)과 비교하였다. 트리티케일은 7.77-9.69%의 단백질을 함유하여 밀과 호밀의 단백질 함량보다 다소 낮았지만, 전분함량이 높아(81.57-85.25%) 에너지원 또는 전분 기반 소재에 활용도가 높을 것으로 생각된다. 신영과 조성의 탄산나트륨 보유능은 각각 72.5와 82.0%로 이는 새금강과 백강과 비슷한 수준이었으며 신조성은 97.6%로 트리티케일 중 제분에 의한 전분 손상이 가장 많이 발생한 것을 알 수 있다. 50% 자당 보유능에서는 조성과 신조성은 신영보다 펜토산에 의한 수화 능력이 높았지만, 트리티케일의 젖산 보유능은 밀과 호밀보다 현저하게 낮아 글루텐 형성에는 취약함을 알 수 있다. 이는 wet gluten의 결과에서 트리티케일의 글루텐형성능이 밀가루의 30-50% 수준이었던 것과도 상응하며 트리티케일 중 신조성이 다른 품종보다 높은 GPI와 wet gluten을 나타내 양적 및 질적으로 가공에 유리할 것으로 생각된다. 조성과 신영은 peak viscosity가 200cp 미만으로 빵용 밀인 백강보다 낮은 반면, 신조성은 백강과 새금강의 중간 수준의 점도를 나타내었고 최종적으로 조성과 신영은 저점도의 페이스트 특성을 보였지만 신조성의 경우 다른 두 품종보다 5배 높은 점도를 가지고 있었다. 트리티케일은 낮은 호화 피크와 호화 엔탈피 값이 관측되었다. α-amylase에 의한 전분 가수분해 특성에서 트리티케일은 밀과 호밀보다 초기 20분 동안 가수분해가 더 많이 진행되었으며 120분이 경과하였을 때 밀과 호밀은 38.2-42.2% 수준인 반면 트리티케일은 47% 이상으로 가수분해 정도가 현저히 높았다. 국내산 트리티케일 품종과 특성에 대한 기초연구와 이를 기반으로 한 가공 연구를 다각적으로 수행한다면 국내 식량작물로부터 새로운 식품소재 창출과 더불어 식량안보문제 해결에도 기여할 수 있을 것이라 생각된다. This study investigated the physicochemical properties of domestic triticale flour and compared it with domestic wheat and rye flour. The triticale flour had a low protein content (7.7-9.7%) but a high total starch and amylose content compared to that of the wheat and rye flour. Shinjoseong showed the highest solvent retention capacity for four solutions (water, Na₂CO₃, lactic acid, and sucrose) among the triticale flour. The wet gluten content of triticale ranged from 13.6- 20.4%, which is lower than that of wheat flour (35.7-39.0%). Joseong showed low viscosity behavior in rapid visco analyzer (RVA), and Shinjoseong showed ranges between Baekkang and Saekeumkang flour. The differential scanning calorimetry (DSC) results showed that Shinyoung had a higher gelatinization temperature and enthalpy compared to that of the other triticale flours. All the triticale flour was hydrolyzed over 50% by α-amylase solution for 120 min, which is higher than wheat and rye flour. This study is expected to provide basic information regarding domestic triticale for the food industry and expand its utility by identifying the physicochemical properties.

      • A Study on the Behavior of Low-concentration Hydrogen on the Hydride Reorientation of Unirradiated Zircaloy-4 Cladding

        Dae-Ho Kim,Hong-Ryoul Oh,Dong-Hak Kook 한국방사성폐기물학회 2023 한국방사성폐기물학회 학술논문요약집 Vol.21 No.1

        In the process of spent fuel dry storage, which is an intermediate management method, it was found that hydrides in the circumferential direction rearranged into radial hydrides. Various factors, such as hoop stress, peak temperature, cooling rate during the storage period, and hydrogen concentration accumulated during the burnup process, significantly affect the susceptibility of spent fuel cladding. In recent studies based on the hydrogen solubility value of about 210 ppm corresponding to the peak temperature of 400°C, if the threshold stress decreases as the hydrogen concentration increases in the low hydrogen range under 210 ppm, the threshold stress increases as the hydrogen concentration increases in the low hydrogen range under 210 ppm. The fundamental cause of this trend is the diffusion of hydrogen into the high-stress region due to the stress gradient formed in the specimen, and hydrogen compounds which remain undissolved in the circumferential direction, even at the peak temperature, play a crucial role to determine the magnitude of the threshold stress. This study evaluated the behavior of hydride reorientation under various hoop stress conditions (70, 80, 90, and 110 MPa) using unirradiated Zircaloy-4(CWSRA) cladding tubes under long-term cooling conditions (3, 6, and 12 months). The results of analyzing the offset strain by hydrogen concentration for long-term cooling showed that specimens with low hydrogen concentration exhibited higher integrity than specimens with high hydrogen concentration at hoop stresses of 90 and 110 MPa. The HR test using irradiated fuel cladding showed that specimens with low hydrogen concentrations exhibited relatively higher susceptibility. To quantify these results, it is necessary to research further in detail by repeated tests.

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