Genetic Diversity and Structure of Cordyceps sinensis Populations from Extensive Geographical Regions in China as Revealed by Inter-Simple Sequence Repeat Markers

Hong-Hui Liang,Zhou Cheng,Xiao-Ling Yang,Shan Li,Zu-Quan Ding,Tong-Shui Zhou,Wen-Ju Zhang,Jia-Kuan Chen 한국미생물학회 2008 The journal of microbiology Vol.46 No.5

Cordyceps sinensis is one of the most valuable medicinal caterpillar fungi native to China. However, its productivity is extremely limited and the species is becoming endangered. The genetic diversity of eighteen C. sinensis populations across its major distributing regions in China was evaluated by inter-simple sequence repeat (ISSR) markers. A total of 141 markers were produced in 180 individuals from the 18 populations, of which 99.3% were polymorphic. The low average of Shannon (0.104) and Nei index (0.07) of the 18 populations indicates that there are little genetic variations within populations. For all 18 populations, estimates of total gene diversity (HT), gene diversity within populations (HS), coefficient of genetic differentiation (GST), and gene flow (Nm) were 0.170, 0.071, 0.583, and 0.357, respectively. This pattern suggests that the genetic diversity of C. sinensis is low and most of the ISSR variations are found among populations with little gene exchange. The 18 populations are divided into five groups based on the genetic distance and the grouping pattern matches with the geographic distribution along the latitudinal gradient. The five groups show obvious difference in the GST and Nm values. Therefore, the genetic diversification of C. sinensis populations may be determined by geographic isolation and the combined effects of life history characters and the interaction with host insect species. The information illustrated by this study is useful for selecting in situ conservation sites of C. sinensis.

Hardness prediction based on microstructure evolution and residual stress evaluation during high tensile thick plate butt welding

Zhou, Hong,Zhang, Qingya,Yi, Bin,Wang, Jiangchao The Society of Naval Architects of Korea 2020 International Journal of Naval Architecture and Oc Vol.12 No.-

Two High Tensile Strength Steel (EH47) plates with thickness of 70 mm were butt-welded together by multi-pass Submerged Arc Welding (SAW), also the hardness and welding residual stress were investigated experimentally. Based on Thermal-Elastic-Plastic Finite Element (TEP FE) computation, the thermal cycles during entire welding process were obtained, and the HAZ hardness of multi-pass butt welded joint was computed by the hardenability algorithm with considering microstructure evolution. Good agreement of HAZ hardness between the measurement and computational result is observed. The evolution of each phase was drawn to clarify the influence mechanism of thermal cycle on HAZ hardness. Welding residual stress was predicted with considering mechanical response, which was dominantly determined by last cap welds through analyzing its formation process.

Investigation on Excitation Equivalence Technique for Thin-Walled Panel Based on Pseudo-excitation Method

Hong-wei Zhou,Qun Yan,Ding-wen Guo,Yan Wang 한국항공우주학회 2022 International Journal of Aeronautical and Space Sc Vol.23 No.4

The new-generation high-speed fighter aircraft primarily utilizes the internal weapons bay structure. When the bay door opens, the level of acoustic load on the structure panel could reach more than 170 dB. As a result, the problem of sonic fatigue is evident in the internal structures of the weapon bay. Nowadays, when conducting ground test, an acoustic noise loading greater than 170 dB is difficult and uneconomic to apply because of limited noise generation ability. An equivalence technique based on the modal superimposition approach and a pseudo-excitation method has been developed to mimic the acoustic loading into an equivalent vibration excitation, and a procedure based on FEM code has been implemented. A single laminated plate structure and a typical built-up panel specimen from internal weapons bay were taken for validation. The results indicate that the response under the derived equivalent vibration excitation shows a good consistency with an error of less than 1% with that under the acoustic load in simulation, while with an error less than 10% in alternative vibration test.

Synthesis, Crystal Structure and Photoluminescent Property of Cadmium(II) Metal-organic Framework with Mixed Carboxylate and Bis(imidazole) Ligand

Zhou, Yong Hong,Tian, Yu Peng Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.9

Ginsenoside Ro, an oleanolic saponin of Panax ginseng, exerts antiinflammatory effect by direct inhibiting toll like receptor 4 signaling pathway

Hong-Lin Xu,Guang-Hong Chen,Yu-Ting Wu,Ling-Peng Xie,Zhang-Bin Tan,Bin Liu,Hui-Jie Fan,Hong-Mei Chen,Gui-Qiong Huang,Min Liu,Ying-Chun Zhou 고려인삼학회 2022 Journal of Ginseng Research Vol.46 No.1

Background: Panax ginseng Meyer (P. ginseng), a herb distributed in Korea, China and Japan, exerts benefits on diverse inflammatory conditions. However, the underlying mechanism and active ingredients remains largely unclear. Herein, we aimed to explore the active ingredients of P. ginseng against inflammation and elucidate underlying mechanisms. Methods: Inflammation model was constructed by lipopolysaccharide (LPS) in C57BL/6 mice and RAW264.7 macrophages. Molecular docking, molecular dynamics, surface plasmon resonance imaging (SPRi) and immunofluorescence were utilized to predict active component. Results: P. ginseng significantly inhibited LPS-induced lung injury and the expression of proinflammatory factors, including TNF-a, IL-6 and IL-1b. Additionally, P. ginseng blocked fluorescence-labeled LPS (LPS488) binding to the membranes of RAW264.7 macrophages, the phosphorylation of nuclear factor-kB (NF-kB) and mitogen-activated protein kinases (MAPKs). Furthermore, molecular docking demonstrated that ginsenoside Ro (GRo) docked into the LPS binding site of toll like receptor 4 (TLR4)/myeloid differentiation factor 2 (MD2) complex. Molecular dynamic simulations showed that the MD2-GRo binding conformation was stable. SPRi demonstrated an excellent interaction between TLR4/MD2 complex and GRo (KD value of 1.16 × 10<SUP>-9</SUP> M). GRo significantly inhibited LPS488 binding to cell membranes. Further studies showed that GRo markedly suppressed LPS-triggered lung injury, the transcription and secretion levels of TNF-α, IL-6 and IL-1β. Moreover, the phosphorylation of NF-kB and MAPKs as well as the p65 subunit nuclear translocation were inhibited by GRo dose-dependently. Conclusion: Our results suggest that GRo exerts anti-inflammation actions by direct inhibition of TLR4 signaling pathway.

Association Between XRCC5, 6 and 7 Gene Polymorphisms and the Risk of Breast Cancer: A HuGE Review and Meta-analysis

Zhou, Li-Ping,Luan, Hong,Dong, Xi-Hua,Jin, Guo-Jiang,Man, Dong-Liang,Shang, Hong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Objective: Non-homologous end joining (NHEJ) is a pathway for repairing DNA double-strand breaks. Recent publications indicated that XRCC5, XRCC6 and XRCC7 genes may participate in the pathogenesis of breast cancer. The aim of this Human Genome Epidemiology (HuGE) review and meta-analysis was to investigate associations between XRCC5, XRCC6 and XRCC7 genetic polymorphisms in the NHEJ pathway and breast cancer risk. Methods: Studies focusing on the relationship between genetic polymorphisms in XRCC5, XRCC6 and XRCC7 genes and susceptibility to breast cancer were selected from the Pubmed, Cochrane library, Embase, Web of Science, Springerlink, CNKI and CBM databases. Data were extracted by two independent reviewers. The meta-analysis was performed with Review Manager Version 5.1.6 and STATA Version 12.0 software. The odds ratio (OR) with 95% confidence interval (95%CI) was calculated based on the extracted data. Results: According to the inclusion criteria, we final included seven studies with a total of 2,864 breast cancer cases and 3,060 healthy controls. Meta-analysis results showed that rs3835 (G>A) and rs828907 (G>T) in XRCC5 gene, and rs132793 (G>A) in XRCC6 gene might increase the risk of breast cancer, while rs132788 G>T and rs6002421 (A>G) might be protective factors. However, there was no relationship between XRCC7 genetic polymorphisms and the risk of breast cancer. Conclusion: This meta-analysis suggests that the rs3835 G>A and rs828907 G>T in XRCC5 gene, rs6002421 (A>G), rs132788 (G>T) and rs132793 (G>A) in XRCC6 gene might be risk factors for breast cancer, while the rs132788 (G>T) and rs6002421 (A>G) in XRCC6 gene might be protective.

Computational analysis of welding radial deformation of typical pressure cylindrical shell with ring stiffener

Zhou Hong,Shen Chaonan,Wang Jiangchao 대한조선학회 2022 International Journal of Naval Architecture and Oc Vol.14 No.1

We report on the evaluation of the radial deformation caused by the welding process on a typical pressure cylindrical shell with ring stiffeners. Welding experiments on butt and fillet welding were conducted beforehand, and temperature-dependent material properties were considered. The out-ofplane welding distortion and residual stress were measured using a three-coordinate measuring machine and X-ray diffraction, respectively. Effective thermal elastic plastic FE analysis based on parallel computation technology was performed to examine the mechanical behaviors. Computed results were validated by comparison with experimental measurements. Welding inherent deformations of the examined butt and fillet joints were then evaluated by integrating computed plastic strains. The elastic FE analysis, with welding inherent deformation as mechanical loading, was employed to examine the dimensional variation of the considered pressure cylindrical shell. The influence of ring stiffener on welding radial deformation was also discussed. Moreover, the application of welding sequence modification and inverse deformation was numerically examined with the proposed elastic FE analysis to reduce the welding radial deformation and enhance the fabrication accuracy.

A STUDY OF CHINESE ANCIENT COMETARY RECORDS

ZHOU HONG NAN,ZHUANG WEIFENG,WANG Yu The Korean Astronomical Society 1996 Journal of The Korean Astronomical Society Vol.29 No.suppl1

From 146 B.C. to A.D. 1760, 363 sets of cometary observations have been recorded in Chinese Ancient Records of Celestial Phenomena. The positions of all recorded comets, or their paths, on the sky are compared. Taking into account the perturbations of all nine planets and using the numerical method of N-body problem, the orbits of well-recorded comets are calculated. Identification of a periodic comet is presented.

Analysis and validation of novel inverter and LCC‑S topology‑based WPT system

Hong Zhou,Haitang Liu,Qijun Deng,Li Fang 전력전자학회 2022 JOURNAL OF POWER ELECTRONICS Vol.22 No.3

Wireless power transfer (WPT) technology has been widely researched and employed due to its advantages when compared with conventional cable-connected power transmission in terms of convenience, flexibility, security, and so on. In common wireless power transfer systems, DC sources, such as batteries and AC–DC modules, are frequently utilized in the front end of the inverters to generate a relatively high frequency circuit driving voltage. The strategy of directly arranging inverters behind AC sources is feasible by replacing a single NMOS with a pair of back-to-back placed switches that can tolerate both forward and reverse voltage. This novel inverter structure is systematically explored from the perspectives of the time domain and the frequency domain, which demonstrates the convenience and feasibility of the proposed structure. Due to the canceling of the front-end rectifier, the complexity of the system hardware is significantly reduced. In addition, when taking the constant current output feature into account, the LCC-S topology is comprehensively investigated and properly applied. Finally, a laboratory platform is constructed and experiments are conducted to verify the effectiveness of the proposed structure. Furthermore, experiment results show that there is only a slight transfer efficiency decrease when compared with the commonly adopted scheme containing an extra diode rectifier at the transmitting side.

A novel ceramic GEM used for neutron detection

Zhou, Jianrong,Zhou, Xiaojuan,Zhou, Jianjin,Jiang, Xingfen,Yang, Jianqing,Zhu, Lin,Yang, Wenqin,Yang, Tao,Xu, Hong,Xia, Yuanguang,Yang, Gui-an,Xie, Yuguang,Huang, Chaoqiang,Hu, Bitao,Sun, Zhijia,Chen, Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.6

A novel ceramic Gas Electron Multiplier (GEM) has been developed to meet the demand of high counting rate for the neutron detection which is an alternative to ³He-based detector at China Spallation Neutron Source (CSNS). An experiment was performed to measure the neutron transmittance of ceramic-GEM and FR4-GEM at the small angle neutron scattering (SANS) instrument. The result showed the ceramic-GEM has higher transmittance and less self-scattering especially for cold neutrons. One single ceramic GEM could give a gain of 10²-10⁴ in the mixture gas of Ar and CO₂ (90%:10%) and its energy resolution was about 27.7% by using ⁵⁵Fe X ray of 5.9 keV. A prototype has been developed in order to investigate the performances of the ceramic GEM-based neutron detector. Several neutron beam tests, including detection efficiency, spatial resolution, two-dimensional imaging, and wavelength spectrum, were carried out at CSNS and China Mianyang Research Reactor (CMRR). The results show that the ceramic GEM-based neutron detector is a good candidate to measure the high intensity neutrons.