류마티스 관절염 환자의 T세포 수용체 Vβ 유전자 레퍼토리 분석

정성수 ( Sung Soo Jung ),황관표 ( Kwan Pyo Hong ),김동욱 ( Dong Yook Kim ),김태환 ( Tae Hwan Kim ),이인홍 ( In Hong Lee ),전재범 ( Jae Bum Jun ),배상철 ( Sang Cheol Bae ),유대현 ( Dae Hyun Yoo ),김성윤 ( Seong Yoon Kim ),이은영 ( 대한류마티스학회 1996 대한류마티스학회지 Vol.3 No.1

목적: 류마티스 관절염(rheumatoid arthritis; RA)의 병인에 있어 중추적인 역할을 하는 것으로 알려진 T세포에 관한 연구가 최근에 관심의 초점이 되고 있으나, 현재까지 한국인을 대항으로 시행된 보고는 없다. 한국인 류마티스 관절염 환자의 관절병변부위에서 T세포 수용체(TCR)의 Vβ유전자 사용의 빈도를 검색하여 한국인 류마티스 관절염 환자에서 사용되는 빈도가 높은 유전자계를 찾는데 목적을 두었다. 방법: 이에 저자는 T세포에 관한 연구의 일환으로 류마티스 관절염 환자 3명과 정상인 4명을 대상으로 말초 혈액과 활액 T세포로부터 추출한 RNA를 이용하여 cDNA를 합성한뒤 cDNA를 주형을 Vβ family specific oligonucleotide를 시발체(primer)로하여 반정량적 역전사 연쇄중합반응(semiquantative RT-PCR)을 시행하여 T세포 수용체(T cell receptor; TCR) Vβ 레퍼토리를 분석하여 다음과 같은 결과를 얻었다. 결과: 1. 정상인 4명의 말초혈액에서 T세포 수용체 Vβ유전자 평균사용빈도는 Vβ7(8.68±3.20%), Vβ3(7.83±2.03%), Vβ(6.74±1.43%)의 순서로 빈도가 높았으며, HLA-DR4 양성군(3명)에서는 Vβ8(7.39±1.71%), Vβ2(7.31±2.30%), Vβ1(7.22±1.54%)의 순서로 빈도가 높았고, HLA-DR4 음성(1명)에서는 Vβ3(17.79%), Vβ4(11.41%), Vβ24(9.8%)의 순서로 사용빈도가 높았다. 2. 류마티스 관절염환자 3명의 말초 혈액에서는 Vβ16(6.90±1.81%), Vβ18(6.89±0.80%), Vβ14(6.58±0.65%)의 순서로 빈도가 높았다. HLA-DR4 양성 환자군에서는 Vβ16(7.52±2.06%), Vβ14(6.96±0.04%), Vβ22(6.78±0.18%)의 순서로 빈도가 높았다. 각각 환자의 결과를 보면 첫번째 환자(HLA-DR4 양성)는 Vβ16(8.98%), Vβ14(6.99%), Vβ22(6.91%)의 순서로 빈도가 높았고, 두번째 환자(HLA-DR13 양성)는 Vβ18(7.79%), Vβ20(5.98%), Vβ24(5.90%)의 순서로 빈도가 높았다. 이를 종합해 보면 류마티스 관절염 환자의 말초혈액에서 Vβ16, Vβ20, Vβ14, Vβ18유전자계가 제한되어 사용되었다. 3. 류마티스 관절염환자의 활액의 T세포에서 Vβ유전자 평균발현빈도는 Vβ20(7.39±1.77%), Vβ18(5.60±1.31%), Vβ12(5.56±1.77%)순으로 높았으며, HLA-DR4양성인 환자군에서는 Vβ12(6.56±0.58%), Vβ20(6.44±0.94%), Vβ4(5.30±0.49%)순으로 빈도가 높았으며, HLA-DR4음성 환자에서는 Vβ20(9.29%), Vβ19(7.57%), Vβ18(7.07%)의 순서로 빈도가 높았다. 각각 환자의 결과를 보면 첫번째 환자(HLA-DR4 양성)는 Vβ12(6.15%), Vβ20(5.77%), Vβ7(5.74%)의 순서로 빈도가 높았고, 두번째 환자(HLA-DR4 양성)는 Vβ20(7.10%), Vβ12(6.98%), Vβ24(5.83%)의 순서이었고, 세번째 환자(HLA-DR13 양성)는 Vβ20(9.29%), Vβ19(7.57%), Vβ18(7.06%)의 순서로 빈도가 높았다. 활액 T세포에서는 Vβ20, Vβ12유전자계가 제한되어 사용되었다. 4. 말초 혈액에 비해 활액 T세포에서 TCR Vβ유전자 사용빈도가 1.5배 이상인 경우는 Vβ5.2, Vβ9, Vβ23 이었으나, 이들 유전자계가 활액 T세포 전체에서 차지하는 사용빈도에 있어서는 각각 Vβ5.2는 1.07±1.26%, Vβ9는 2.17±1.42%, Vβ23는 3.84+1.97%로 낮은 수치를 나타내었다. 각각 환자에서 비교했을때에 첫번째 환자(HLA-DR4 양성)는 Vβ23(3.69%:1.12%), Vβ5.1(3.51%:1.40%), Vβ12(6.15%:3.29%), Vβ6(3.35%:2.10%), Vβ2(5.74%:3.73%)의 유전자계에서, 두번째 환자(HLA-DR4 양성)는 Vβ9(3.58%:0.74%), Vβ19(4.67%:1.52%), Vβ5.2(2.52%:0.87%), Vβ10(4.25%:1.75%), Vβ1(5.74%:2.35%)의 유전자계에세, 세번째 환자(HLA-DR13 양성)는 Vβ6(2.68%:0.15%) Vβ9(2.17%:0.73%), Vβ11(2.30%:1.10%), Vβ20(9.29%:5.98%)의 유전자계에서 활액에서 말초혈액보다 1.5배이상 증가되어 사용됨을 볼수 있었다. 즉 활액에서 말초혈액보다 의미 있게 편향되어 사용되는 Vβ 유전자계는 각각 환자마다 다른 결과를 나타내었다. 5. 류마티스 관절염환자의 말초 혈액과 정상인의 말초 혈액의 T세포의 Vβ 유전자 사용빈도를 비교했을때 류마티스 관절염 환자의 말초혈액 T세포에서 Vβ16(6.90±1.81%:3.23±1.21%), Vβ18(6.89±0.80%:2.72±0.70%) 유전자계가 2배 이상으로 빈도가 높았고, HLA-DR4양성군만 비교하였을때도 Vβ16(7.52±2.06%:3.69±1.46%), Vβ18(6.45±0.28%:2.61±0.86%) 유전자계가 2배 이상 사용이 많았다. 결론: 이상의 성적으로 미루어 보아 류마티스 관절염 각각 환자마다 다른 Vβ 유전자계의 제한적 사용과 편향됨을 볼 수 있었으나, 공통된 유전자계의 증식은 볼 수 없었다. 이러한 결과는 다른 연구보고와는 다른 결과를 보였고, 이것은 대상 환자의 질병의 유병기간이 다르고, 또 유전적인 배경, 생존 환경, 적용된 방법의 차이로 기인한다고 하는 기존의 보고와 부합된다고 사료되는데 질병의 진행단계에 따라 주로 면역반응을 일으키는 항원의 에피토프가 달라짐에 따라 여기에 대항하는 T세포들의 수용체도 이들 에피토프에 반응할 수 있는 수용체를 가진 코론들이 증식하게 된다는 epitope spreading theory에 부합되며, 이는 아주 초기에 질병을 시작하게 유도하는 항원의 특성을 규명하기 위해서는 환자선택이 연구결과에 결정적인 역할을 할 것으로 사료된다. 또 다른 가능성은 각각 환자마다 다른 Vβ유전자계의 증식된 클론들의 CDR3 연기 서열을 규명하여 비록 유전자 서열이 다를 지라도 항원과 결합하는 같은 성상을 갖는 아미노산 motif를 가질 가능성에 대해서도 연구가 필요하리라 사료된다. Objectives: Polymerase chain reaction (PCR) technology was eamine synovial fluid and peripheral T cells in patients with rheumatoid arthritis (RA) to determine the preferential usage of the T cell receptor(TCR) variable region (V) gene. Methods: Oligonucleotide primers specific for individual TCR Vβ gene families were used to amplify the TCR gene products in a semiquantitative assay of their relative utilization in unselected T cell populations. Results: The result of Vβ utilization was generally heterogenous, similar with previous reports. However, the mean expression of Vβ16 and Vβ18 in RA was more preferentially utilized compared to normal donors. The usage of Vβ in peripheral blood from 3 patients with RA demonstrated restrictions in Vβ16, Vβ20 and Vβ18 genes, respectively. Analyses of synovial fluid resulted in restriction in β12, Vβ20 and Vβ20, respectively. Although there was no significant pattern of skewed Vβ gene mean usage when comparing the synovial fluids with the peripheral blood T cells from RA patients, there were significant biased Vβ genes, Vβ12, Vβl and Vβ20, each 3 patients. As the HLA type is a determining factor in shaping TCR repertoire of peripheral T cells, we compared the Vβ utilization in HLA-DR4 expressing groups that have susceptibility and gene dosage effect in disease progression. It was a little different that comparing the pattern of Vβ usage in peripheral blood and synovial fluid from RA patients between HLA-DR4 positive and negative group. Conclusion: The results were consistent with the conclusion that the increased Vβ family T cells infiltrate synovium and are dependent on each patient and may be involved in inducing and maintaining the synovitis that characterizes RA The different outcome of each patient may be due to the difference in disease duration, genetic background and geographic region. A more important factor may be the stage of disease, because epitope induced immune reaction may change over time. Therefore, selecting patients early in the course of disease may be important and may facilitate the need for more in-depth TCR analysis in the future.

고식적 체외수정시술과 난자 세포질내 정자주입술에 의해 태어난 아이의 주산기 결과 및 선천성 기형 발생빈도의 비교 연구

임정은,유근재,이종표,이문섭,현우영,전진현,홍수정,송지홍,송인옥,백은찬,최범채,손일표,궁미경,강인수,전종영,박인서,Lim, Jeong-Eun,Yoo, Keun-Jai,Lee, Jong-Pyo,Lee, Moon-Seob,Hyun, Woo-Young,Jun, Jin-Hyun,Hong, Soo-Jeong,Song, Ji-Hong,Song, In-Ok,Paik, 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.3

The safety of ICSI as a novel procedure of assisted fertilization may be assessed by the health of the baby born. In order to evaluate the safety of ICSI, perinatal outcome and congenital anomaly of the babies born after ICSI were compared with those of babies born after IVF (control group). We analysed the clinical data from the obstetric and pediatric records, including the information obtained through telephone. The results are as follows; Mean gestational age $({\pm}SEM)$ and birth weight in singleton pregnancy were $38.8{\pm}1.9$ weeks and $3209.7{\pm}501.9gm$ in IVF group, $39.0{\pm}2.2$ weeks and $3289.9{\pm}479.5gm$ in ICSI group, respectively. Mean gestational age and birth weight in twins were $36.8{\pm}2.1$ weeks and $2512.8{\pm}468.0gm$ in IVF group, $36.5{\pm}2.8$ weeks and $2492.7{\pm}537.1gm$ in ICSI group. In IVF group, perinatal mortality rates were 8.5 in singletons and 56.6 in twins; for the ICSI singletons and ICSI twins, the perinatal mortality rates were 11.6 and 49.0, respectively. The incidence of congenital malformations was 3.6% (8/224) in IVF group and 2.1% (4/188) in ICSI group, there was no statistical difference (p>0.05, Fisher's exact test). The incidence of major congenital anomalies was 0.9% (2/224; pulmonary artery hypoplasia, renal cystic dysplasia) in IVF group and 1.1% (2/188; holoprosencephaly, Cri du chat syndrome) in ICSI groups (p>0.05, Fisher's exact test). Similarly, there was no significant difference in incidence of minor congenital anormalies 2.7% (6/224) in IVF group and 1.1% (2/188) in ICSI group respectively (p>0.05, Fisher's exact test). In conclusion, there was no difference in the perinatal outcome and the incidence of congenital anomalies between the babies born after ICSI and those after conventional IVF.

β-Lactamase생성균주에 대한 새로운 β-Lactamase Inbitating Compound와 β-lactam항생제와의 병용시 항균효과

홍인표,김홍진,임채욱,김기호 중앙대학교 약학연구소 1996 약학 논총 Vol.10 No.-

The in vitro and in vivo activities of CH1250, a new β-lactamase inhibiting compound, were compared with the activities of clavulanic acid and sulbactam against 30 β-lactamase-producing bacteria. Broth microdilution susceptibility testing was performed to compare the activities of β-lactamase inhibitors. Serial two fold dilutions of β-lactams were tested alone and in two combinations with β-lactamase inhibitor. CH1250 was more active than clavulanic acid against Acinetobacter calcoaceticus S21, Pseudomonas aerug-nosa GN918, Proteus vulgaris 20, Staphylococcus aureus MS15009/1258, Serratia marcescens 1 when combined with cefotaxime. When combined with amoxicillin, CH1250 was more effective against Citrobacter diversus 2046E. Staphylococcus aureus MS25009/1258, Proteus vulgaris GN76. With ampicillin, CH1250 had an better or simillar activities against Escherichia coli 3455E, Klebsiella aerogenes 1976E, Serratia marcescens 1, Staphylococcus aureus MS15009/1258. The efficacy of ampicillin-CH1250 in treatment of mice with systemic infections produced by Klebsiella aerogenes 1976E, a β-lactamase-producing bacteria, was compared with that of ampicillin, ampicillin-sulbactam. Acute systemic infections in mice were produced by i.p. inoculation of bacterial cultures suspended in 6% mucin. Mice were treated s.c. 1hr after challenge. PD50s of the ampicillin, ampicillin-sulbactam. ampicillin-CH1250 were 334.32, 170.58, 250.5mg/kg, respectively. CH1250 was not more effective than sulbactam in reducing ampicillin doses required to protect mice from infection produced with Klebsiella aerogenes 1976E. The reduction in the 50% protective dose of ampicillin by CH1250 was 23% better than those effected by sulbactam for Klebsiella aerogenes 1976E infection.

혈액 내 구리, 아연 및 ceruloplasmin 농도에 흡연, 음주 및 신체적 활동이 미치는 영향

홍연표,강은용,신인철,최병선,박정덕,장임원,박진완 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.4

Objectives : To investigate the influence of smoking, alcohol ingestion, and physical activity on copper and zinc in RBC and serum and serum ceruloplasmin, this study was performed in a cross-sectional study in 113 healthy men aged 20 to 40 years who had no symptomatic liver, heart, gastrointestinal, and other chronic diseases. Methods : At the men's entry into the study, blood samples were drawn from each subject and immediately centrifuged for analysis of copper, zinc, iron, ceruloplasmin, total cholesterol, and hematocrit. Each man completed a questionnaire that provided information on smoking, amount of alcohol intake, and physical activity. Partial regression analysis was performed on confounding variables such as age, body mass index, hematocrit, serum cholesterol, and serum iron. Results : In general linear models, adjustment for confounding variables did not show statistical differences, and there was only an increasing tendency in serum copper in heavy smoker(P=0.0678). There was no difference between high physical activity with mild smokers and lower physical with heavy smokers. Conclusions : This study suggested that copper, zinc and ceruloplasmin were not good biomarker for early effect by smoking, alcohol intake and physical activity in young adult. However, selection bias should be considered in evaluation of this result, and a large prospective study will be needed in advance on usefulness of copper, zinc and ceruloplasmin as a marker for risk factors and early change of atherosclerosis.

카드뮴 정맥주사시 랏트의 치사율과 간, 신에서의 양-반응관계

김인봉,박정덕,홍연표,장임원 중앙대학교 의과대학 의과학연구소 1992 中央醫大誌 Vol.17 No.4

To investigate the lethality and dose-response relationship in liver and kidney of rats at 48 hours after cadmium exposure, single doses of cadmium chloride were given to Sprague-Dawley male rats by tail-intravenous injection with several groups of doses ranging from 2 to 8 mg CdCl_2/kg for lethality and from 0.2 to 3.2 mg CdCl_2/kg for dose-response relationship. In addition, control was treated with distilled water by same method. Hepatic and renal cadmium concentrations were analyzed with I.C.P.(Inductively Coupled Plasm) Emission Spectrometry (Jobin Yvon, Model JY 24, France). Dosages over 4 mg/kg were relevant to LD_100 and LD_50 was calculated as 3.3 mg/kg at 48 hours after cadmium chloride exposure in rat. The concentration in liver and kidney were increased according to given doses, and dose-response relationship was expressed as log Cd in liver= 0.8799+0.913×log Dose (F=1116.1, r=0.9877) and log Cd in kidney= 0.2542+0.0653×log Dose (F=884.8, r=0.9845), respectively. Also, the correlation between hepatic and renal cadmium concentration was highly significant(p<0.01), and dose-response relationship was expressed as long Cd in kidney = -0.6649+1.0259×log Cd in liver (F=411.1, r=0.9676). I consider that this data will be useful for the further studying of low-dose cadmium toxicity.

금강의 수질, 어패류, 이끼 및 토양중의 중금속의 함량 조사

한인전,홍춘표,최용규 공주대학교 사범대학 과학교육연구소 1993 과학교육연구 Vol.24 No.1

This survey is to study the corresponding degree of water pollution of the Kum river areas, covering the eight places of main stream of the river from the Daechung dam to Kangkyung county, and two trivutary streams of Kap stream, and Miho stream. Besides, the heavy metal content of the fishes, shellfish, moss, and soil in the main stream of Kum river is investigated, whose results are as follows: 1. The water quality The water quality of the area from the Daechung dam to the Sintanjin county is first grade for the standard of drinking water in terms of BOD, COD values, that of both Kap stream and Miho stream is third or fourth grade respectively, and the other areas are turned out to be second grade. The water of the Daechung dam to the Sintanjin has little NO₂-N and ??, which can be said to be possible for the drinking water. The quantity of ?? and ?? in the water of all the areas is below the standard for the available drinking water, but, that of Kap stream and Miho stream is about two or three times higher than other areas. 2. Heavy metal The content of Cd, Cu, Mn, and Zn are shown to be comparatively less than that of Co, Fe, Ni, and Pb. And the content of Cu, Mn, Zn are less than the possible standard value for the drinking water. The relationship of the heavy metal content of the fish and shellfish were in order of Co>Zn>Cu>Cd, for the moss, Zn>Pb>Co>Cu>Cd, and for the soil, Zn>Pb>Cu>Co>Cd. The most of collecting samples in the Kumnam area have lots of heavy metals, and Pb is well contained in shells than fishes. When summarize the above result, the water in the Daechung dam and Sintanjin area is comparatively favorable for the drinking water, and the water quality below the Kumnam areas appears to be wores because of influx of Kap stream and Miho stream. In short, the necessary counter measure is to be required for the good preservation of water of fine quality in Kum River.

Sow Transfer of Cultured Embryos : Embryo Recovery, Oocyte Collection, IVM-IVF-IVC Treatment, Vitrification/Thaw, and Surgical and Nonsurgical Transfer

Kim In-Doc,Ahn Mi-Hyun,Hur Tae-Young,Son Dong-Soo,Hong Moon-Pyo,Seok Ho-Bong 한국발생생물학회 2003 한국발생생물학회 학술발표대회 Vol.2003 No.1

The aims of this study are 1) to test oocytes and embryos collected from in-vivo and in-vitro to achieving the valuable protocol by culturing, vitrifying and thawing of oocytes/embryos, and 2) to transfer them to recipient, and finally have resulted in pregnancies from recipient females after surgical or nonsurgical transfer. In vitro maturation and fertilization were performed according to the procedures of Funahashi et al. Fertilized oocytes were cultured in glucose-free NCSU 23 supplemented with 5 mM sodium pyruvate, 0.5 mM sodium lactate and 4 mg/ml bovine serum albumin for 2 days at 39, and 10% fetal bovine serum was added to the culture medium thereafter. Embryos were treated with 7.5g/ml cytochalasin-B for 30 min, centrifuged at 13,000 g for 13 min and then exposed sequentially to an ethylene glycol (EG) vitrification solution, aspirated into OPSs, and plunged/thawed into/from liquid nitrogen. In vivo embryos were surgically collected from three donors after Al. Forty-six embryos (18, 9 and 19 embryos, respectively) were washed 3 times in mPBS+10%FBS, followed treatments : cultured, centrifuged, vitrified, recovered and transferred to recipients as in vitro prepared embryos. Three recipients received surgically 34(control), 188 and 184 embryos (derived from abattoir), respectively. Another three recipients were received nonsurgically 150, 100 and 150 embryos, respectively. All recipient sows exhibited delayed returns to estrus. To our knowledge, these results suggest that required an improved techniques, more vigorous embryos preparation and cleaner uterous condition(use gilt).

Poster Session : PS 1510 ; Rheumatology : Postoperative Infection Rates after Surgery with Prosthesis in Patients with Ankylosing Spondylitis Treated by TNF Alpha Blockade Compared to Conventional NSAIDs

( Ki Pyo Kim ),( Joon Young Hur ),( Ji Young Choi ),( Ran Song ),( Sang Wan Chung ),( Yeon Ah Lee ),( Seung Jae Hong ),( Hyung In Yang ),( Sang Hoon Lee ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Some patients with severely advanced ankylosing spondylitis (AS) need to replace or correct destructed joints by surgical treatment. Among these patients, some patients who don’trespond sufficiently to conventional NSAIDs have to be treated by anti-TNF alpha blockades. We reviewed these patients to assess postoperative infection rate after surgery with prosthesis. Methods: We retrospectively reviewed the medical records of the patients with AS who experienced the surgery with prosthesis in Kyung Hee university hospital at Gangdong in Seoul, South Korea from Mar 2006 to May 2014. Results: Total 307 patients underwent the surgeries such as total hip replacement (THR) or corrective osteotomy of spine. Among them, 25 patients have been treated by TNF alpha blockades after surgery. 9 patients had been treated by TNF alpha blockade before the surgery. Total follow-up period was 4.3±2.3 years per person. Their average ages for surgeries are 35.6± 10.7 years. Among them, 13 patients had THR and the others had corrective osteotomy in spine. 11 patients have been treated by adalimumab, 9 patients have been treated by etanercept and 5 patients have been treated by in. iximab. There was no wound complication associated with infection after the surgery during follow-up period in the group of patients treated by TNF alpha blockade. But in the group of patients who had been treated by conventional NSAIDs, one patient had wound infection after spine surgery. His age was 62 and he had uncontrolled DM. Conclusions: There is no increased risk of infection after the surgeries using prosthesis in patients with AS treated by TNF alpha blockade compared to the patients treated by conventional NSAIDs.

錦江上流地域에 있어 다슬기科의 分布에 關한 硏究

金鍾煥,崔信錫,宋仁植,洪榮杓 충남대학교 자연과학연구소 1982 忠南科學硏究誌 Vol.9 No.1

The distribution and identification of Semisulcospira species were studied at the 8 stations in the upper streams of Geum River and it's tributatries, and some physicochemical analysis were obtained from all stations. The results are summerized as follows: 1. Four species of Semisulcospira were collected from the 7 stations in the 8 stations, except a station, the Buchang stream. S. coreana was found at all the 7 stations, S. ovulum at the 6 stations, S. globus at the 4 stations, and S. tegulata was found only two stations, but the density of all the snails was generally low in a m^2. 2. Identification of the snail species were based on the external morphology, radula ribbon and central teeth patterns. 3. The chromosome numbers were observed : n = 18 in S. coreana and S. ovulum ; n = 16 in S. globus, however, in S. tegulata the chromosome was not observed. 4. Physicochemical analysis in the aquatic environment were determined from all the 8 stations. The pH, Ca, Mg, SO_4, Na and Cl values in the water varied not widely, but the values of chemical oxidation demand at the 5th station and electric conductivity at the 7th station were higher than those of the another stations.

다층지반에서의 아칭현상에 의한 수직갱 토압

이인모,문홍표,이대수,김경렬,조만섭,Lee, In-Mo,Moon, Hong-Pyo,Lee, Dea-Su,Kim, Kyung-Ryeol,Cho, Man-Sub 한국터널지하공간학회 2007 한국터널지하공간학회논문집 Vol.9 No.1

본 연구에서는 사질토 지반에 설치된 수직갱에 작용하는 토압에 대한 기존 제안식을 수정하였다. 이를 검증하기 위하여 깊이에 관계없이 동일한 반경방향 변위를 일으킬 수 있는 실내 모형시험장치를 개발하였으며, 벽면마찰각과 상대 밀도를 변수로 시험하였다. 실내 모형시험 결과는 수정식에서 접선방향 토압계수(수직응력에 대한 접선방향 응력 비)인 $\lambda$값을 $\lambda=1-sin\phi$와$\lambda=1$을 사용하였을 경우의 토압 사이에 분포하였다. 다층지반에 설치된 원형수직갱 배면지반의 파괴면을 가정하고 수정식을 적용하여 다층지반에 설치된 원형수직갱에 작용하는 토압 산정방법을 제안하였다. 시공현장의 3개의 수직갱으로부터 계측 데이터를 획득하였으며 이를 토압으로 환산하여 제안된 방법을 검증하였다. 계측 데이터로부터 환산된 대부분의 환산 토압은 제안된 방법의 토압과 잘 일치하였다. A new earth pressure equation acting on the vertical shafts in cohesionless soils has been proposed by modifying the equations proposed by others. In order to verify the modified equation, model tests which can control uniform wall displacement with depth to radial direction were conducted. Model tests were performed with three different wall friction angles and two different relative densities. The measured values were larger than estimated values when assuming $\lambda=1$ ; smaller than those when assuming $\lambda=1-sin\phi$. The parameter, $\lambda$ is the ratio of tangential stress to vertical stress and is the most critical value in proposed equation. A method which can estimate the earth pressure on vertical shafts in layered soils is also proposed by reasonably assuming the failure surface of layered soils and using the modified equation. In order to verify the proposed method, in-situ measurement data have been collected from the three in-situ vertical shafts installed in layered soils. Most of earth pressures converted from measured data match reasonably well with estimated values using proposed method.