Negative Regulation of Hypoxic Responses via Induced Reptin Methylation

Jason S. Lee,김윤호,김익수,김보규,최희준,이지민,신희재,김정화,김지영,서상범,이호,Olivier Binda,Or Gozani,Gregg L. Semenza,김민형,김근일,황대희,백성희 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

AH-66 Hepatoma 세포의 Histone에 관한 연구

최종오,이동욱,이희성 중앙대학교 의과대학 의과학연구소 1985 中央醫大誌 Vol.10 No.2

In attempt to investigate histone fractions of tumor cells, histone fractions HI, H2a, H2b, H3 and H4 were prepared from isolated nuclei of AH-66 hepatoma cells and histone fractions were characterized by amino acid analysis and by polyacrylamide disc gel electrophoresis. The results obtained were as follows; 1, The five histone fractions found in most tissues were also present in the AH-66 hepatoma cell histories. 2. The yield of whole histone was 6.46mg per g of packed cells. 3. The yield of DNA was 14.78mg per g packed cells. Consequently the DNA to histone ratio was 1:0.44. 4. The relative amounts of five fractions, i.e., H1, H2a, H2b, H3 and H4 were 15.64% 34.83%, 21,21%, 11.76% and 16.56%, respectively. The data presented in this report show that there is a considerable variation in the relative amounts of histone fractions as compared to those of calf thymus. 5. The electrophoretic mobility of individual histone fractions gave almost similar patterns to those of corresponding fractions of calf thymus. 6. Amino acid analysis of the individual histone fractions showed that the over-all compositions were similar but not identical to those of corresponding fractions from calf thymus and other tumor cells. 7. Histone fractions HI, H2b. H3 and H4 were found to contain detectable amounts of ε-N-monomethyllysine. Usually the methylated derivatives of histidine have been reported in histone Hi fraction of Sarcoma 180 ascites tumor cells and Walker 256 carcinosarcoma. However, the methylated histidine residue was not found in all histone fractions from AH-66 hepatoma cells. 8. The evidence was presented that AH-66 hepatoma cells contained five major histone fractions which were homologous to corresponding five fractions of calf thymus.

Negative Regulation of Hypoxic Responses via Induced Reptin Methylation

Jason S. Lee,김윤호,김익수,김보규,최희준,이지민,신희재,김정화,김지영,서상범,이호,Olivier Binda,Or Gozani,Gregg L. Semenza,김민형,김근일,황대희,백성희 Sookmyung Women's University Research Institute of 2010 여성과 건강 Vol.6 No.1

中·高等學校 體育管理의 實態調査 硏究 : 全羅北道를 中心으로

李濟炯,柳尙秀,姜熙成 全北大學校 師範大學 1980 사대논문집 Vol.6 No.-

Ehrlich 복수암 세포의 지질대사에 관한 연구

이희성,하창우,이근배 중앙대학교 의과대학 의과학연구소 1977 中央醫大誌 Vol.2 No.1

We have studied on lipids of Ehrlich ascites tumor cells (EATC). Lipids were extracted by Kennedy and Kanfer(1964) method and lipoproteins were separated by paper electrophoresis. For the identification and assay of lipid extracts, thin-layer chromatography was employed. The incorporation of ^32P-orthophosphate into phospholipids of EATC was also observed. The results obtained were summarized as follows; 1. The total lipid content of EATC was 47.15 ㎎ per g wet weight; of this 32.2㎎ per g was neutral lipid and 14.95 ㎎ per g was phospholipid. 2. The amounts of neutral lipid fractions of EATC, i.e., free cholesterol, esterified cholesterol, mono-, di-, triglyceride and free fatty acid were 2.06㎎, 1.75㎎, 4.71㎎, 7.15㎎, 10.74㎎ and 2.21㎎, respectively. The amount of triglyceride of EATC was similar to those of normal mouse liver. 3. Phospholipid composition of EATC was phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and unidentified phospholipid, and contents of the individual phospholipids showed that the over-all composition were very similar to normal mouse liver. 4. Lipoprotein fractions of EATC were chylomicron, β-lipoprotein, α-lipoprotein, and prealbumin. Chylomicron fraction contained a larger amount of lipid and α-lipoprotein fraction contained a smaller amount of lipid. 5. The incorporation of ^32P into phospholipids reached a maximum 5 minutes after incubation.

자연 방사선량 중 ²²²Rn 기여 성분 정량 해석

이병영,채하석,전재식,오희필,김도성 대한방사선 방어학회 1989 방사선방어학회지 Vol.14 No.2

옥외 라돈이 호흡기관에 주는 선량을 측정·평가할 목적으로 CR-39 비적검출기를 내장한 라돈컵을 사용하여 대기중 라돈농도를 측정하였다. 직접형 검출기 및 개방 컵과 필터 컵의 구조를 갖는 CR-39비적검출기에 대한 라돈검출인자는 공기중의 농도가 잘 알려진 표준라돈 조사시설에서 이들 검출기와 라돈컵을 일정기간 조사하여 결정하였다. CR-39를 70°c, 30% NaOH용액으로 220분간 화학부식하였을 때 직접형 거출기와 개방 컵, 필터 컵에 대한 라돈검출인자는 각각 0.273, 0.0813, 0.0371tr mm-2/37 Bqm-3·d)였다. 또한 1988년 5월에서 1989년 3월까지 대전(충남대학교)에서 측정한 대기중의 라돈농도는 개방 컵에 의한 결과는 27.4 - 135.8Bq/m3(0.74 - 3.67 pCi/l)로서 연평균 73.3 Bq/m3(1.98 pCi/l)이었으며, 필터 컵에 의한 결과는 16.7 - 143.9 Bq/m3(0.45 - 3.89 pCi/l)로 연평균 68.5 Bq/m3(1.85 pCi/)이었다. 측정한 옥외 대기중이 라돈농도와 부위별 폐선량모형으로부터 산출한 ICRP표준인의 호흡기관에 대한 실효 선량당량률은 약 520 nSv/h로 평가되었다. A study for the assessment of dose given by outdoor radon to respiratory system has been carried out by making use of radon-cups containing CR-39 plastic track detectors. Detection efficiencies were determined by irradiation of the radon-cups in a standard radon chamber of known concentration. Thus determined detection factors of CR-39 plastic track detector in bare, open cup and filtered cup geometry are found to be 0.273, 0.0813 and 0.0371 trmm-2/(37 Bqm-3 d), respectively, which are chemically etched in 30% NaOH solution of 70℃ for 220 minutes. The outdoor radon concentrations measured at Taejeon (Chungnam National University) from May 1988 to March 1989 are in the range of 27.4 - 135.8 Bq/m3 (0.74 - 3.67pCi/l) by open cup and 16.7 - 143.9 Bq/m3 (0.45 - 3.89pCi/l) by filtered cup, which yield overall annual average value of outdoor radon concentration of 70.8 Bq/m3 (1.91 pCi/l). Corresponding effective dose equivalent rate to respiratory system of ICRP standard man is assessed to be 520nSv/h.

Ferrous Oxalate Dihydrate부터 다공질 철분말의 제조 및 소결성

이상엽,이동희,이재영 연세대학교 산업기술연구소 1989 논문집 Vol.21 No.2

A systematic study on the production of fine iron powders containing numerous micro-pores by reduction of ferrous oxalate dihydrate and also on the sintering behavior of their powder compacts to impart uniformly dispersed fine pores in the sintered mass has been carried out. Very fine porous iron powders(B.E.T. size≤0.4㎛, specific surface area 15m²/g) produced from ferrous oxalate dihydrate were resulted from the evolution of gases during reduction process. Powders reduced around 400℃ for 30∼60 min, showed the good sinterability due to a kind of the activated sintering even at low sintering temperature (700-800℃), mainly because of lager surface area and unreacted inclusions.

학습자 중심 질 좋은 교육 평등화를 위한 조건정비에 관한 연구

이성희 참마음교육학회 2006 未來敎育硏究 Vol.13 No.2

본 연구는 우리 나라 초·중등학교의 현장은 학습자들에게 질 좋은 교육을 평등하게 베푸는데 필요한 교육적 조건과 환경이 너무 열악하기 때문에 교수-학습의 전개과정에서 전인교육을 충실하게 실천하는 것은 어려운 실정이다. 따라서 학교교육을 정상화하고 교수-학습의 전개과정에서 질 좋은 교육을 평등화하기 위해서는 학생 각자가 언제, 어디서나 자기에게 알맞은 것을 제때에 배우고 익히게 하는 수단과 조건, 그리고 그것들을 적절하게 활용하는 절차와 방법 등이 정비되어 있어야 하는 것이다. 이를 위하여 다음과 같은 연구의 내용을 구체적으로 선정하였다. 첫째, 학습자 중심 질 좋은 교육 평등화를 위한 조건 정비와 관련되는 개념체계는 인간교육학의 입장에서 정립된 다음 세 가지를 고찰한다. 둘째, 학습자 중심 질 좋은 교육 평등화를 위한 조건 정비의 실천 체제가 학교교육의 교수-학습 전개과정에서 갖는 활용 타당성과 실천 가능성은 다음 두 가지 차원에서 실습을 통하여 실증적으로 분석·확인한다. 본 연구를 수행하는 데 있어서 사용한 연구의 방법은 크게 두 가지이다. 첫째는, 본 연구의 주제인 학습자 중심 질 좋은 교육 평등화를 위한 조건 정비에 관한 개념 체계와 실천 체제를 구명·고찰하는 데는 관련 문헌과 연구자료, 그리고 이들에 관계되는 선행연구들을 확인·분석하는 문헌조사 연구방법을 사용하였다. 둘째는, 연구자 자신이 체험적 현장 실습을 통한 실증적 분석방법을 사용하였다. 본 연구자는 인간교육학에 더하여 새로 고안·개발된 교육용 두뇌로봇(BRED)이 학교교육의 교수-학습 전개과정에서 실제적으로 학습자 중심의 질 좋은 교육 평등화를 위한 조건 정비의 기능과 역할을 제대로 할 수 있는지, 그리고 그것의 활용 타당성과 실천 가능성은 어느 정도일 것인지를 교육용 두뇌로봇(BRED)의 발명자와 직접 일대일의 교호작용을 통해서 체험적·실증적으로 연구하는 방법을 사용하였다. It is not easy to conduct the child-centered education during the progress of teaching and learning, since there is a lack of Equalization of Excellence Education condition and the environment to give the opportunities for Equalization of Excellence Education in elementary and middle schools. Therefore, students themselves should be prepared for a way and the condition that they could learn things anytime, anywhere with appropriate steps and methods. Two purposes were set as follows in order to fulfil the requirements for the study that was previously indicated. Firstly, conceptual system on the Equalization of Excellence Education should be investigated, which is focused on the learner's point of view. The conceptual system can be conceptually complied as a factor that realize the ideal humanism in the progress of the education and the educational concept by Hong-ik concept. Secondly, the reasonability of the application of actual performing system and the possibility of actual performance on the preparation of the condition for equalization of Excellence Education during the progress of teaching and learning in elementary and middle school education should be analyzed and confirmed through the actual experiments. The specific contents were selected as follows to achieve the purpose of this study. Firstly, the conceptual system related to the preparation of the condition for equalization of Excellence Education focused on the learner considers the 3 factors listed below. A. The equalization of Excellence Education that is focused on the learner B. The measurement of the learning characteristics of learners C. The preparation of learning condition of leaner Secondly, the reasonability of the application and the possibility of actual performance in the progress of teaching-learning stage at school derived from the practice of condition preparation for the Equalization of Excellence Education can be analyzed and actually proved as in the following two factors. A. Analyzing the examples of learner's learning style B. Actual experiment and analyze of the preparation of the condition for equalization of Excellence Education education that is focused on the learner The method of the study can be briefly divided into two parts. One is document research method and the other one is actual evidential analysis through the experiment practice. The conclusion of the study based on the purpose, contents, and the method can be suggested as follows. Firstly, as the result of the actual analysis of the distribution of learning characteristics of learners, the learning characteristics can be divided into 8 categories. This distribution confirmed that learning characteristics are various in the progress of teaching-learning that is occurred between the teacher and student, and students could independently study according to their learning characteristics. Secondly, after the experiment of making various learning subjects(one examination) on 31 students in one class was conducted, based on the learning contexts in the math textbooks for elementary students, it took 217 hours to make the learning subjects that students would apply later on. This explains it is impossible that teacher can create various learning subjects based on students' learning characteristics, while the teacher is performing general tasks. Thirdly, the results as below were found through the experiment using Brain Robat for Education BRED. Learners can find out their individual learning characteristics during the progress of teaching and learning in school education. Learners can select the appropriate learning subjects by themselves. Therefore, they could figure out the results by themselves right away. Also, teachers can understand each student's learning characteristics as well as the results of learning for the whole class objectively.

성격평가 질문지의 불안척도 요인구조 : 정신과 환자집단 자료를 중심으로

이숙희,박민철,이상열,오상우 圓光大學校 醫科學硏究所 2005 圓光醫科學 Vol.20 No.2

Stuides on Histone ( Ⅵ ) : Histones from Normal and Myelocytic Leukemia Leucocytes

이근배,이희성,전재수,최재득 생화학분자생물학회 1980 BMB Reports Vol.8 No.1

The five major fractions of histone from normal and myelocytic leukemia leucocytes were prepared by the procedure of Johns. Electrophoretic behaviors on acrylamide gels and amino acid composition of various histone fractions from normal and leukema leucocytes: were compared:: The results obtained were as follows 1. The yields of whole hisfome isolated from normal and : inyelocytic leukemia leucocytes recovered were 37.08㎎ and 58.64㎎ per lg packed cells, respectively. 2. A marked increase in the amount of fraction KAS (f2b) from leukemia leucocytes was observed. These events were apparently correlated with a parallel decrease in the fractions KAP (f1) and ARE (f3). The .amount of fraction fZa (GRK+LAK) was almost to those of corresponding fraction of normal leucocytes. 3. Amino acid analysis showed the fraction ARE (f3) from leukemia cells contained markedly greater amounts of e-N-monomethyllysine and e-N-dimethyllysine than that from the normal cells.