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Streptomyces sp. GCA0001로 부터의 신규 항생물질 Cystocin의 구조분석, 생물활성 및 유도체제조
김자용,이희찬,우진석,송재경 호서대학교 반도체제조장비국산화연구센터 2001 반도체장비학술심포지움 Vol.2001 No.-
본 발명의 Puromycin 유도체인 Cystocin 화합물은 유기 합성에 의해 제조된 물질이 아니라 방선균계열의 신균주인 Streptomyces sp GCA0001로부터 추출된 신규 물질로서, 항박테리아, 항종양 및 항바이러스 활성 등의 생물학적 미생물 활성면에서 종래의 Puromycin 화합물에 비해 현저히 뛰어난 효과를 지니고 있고 Streptomyces sp GCA0001로부터 추출, 분리 및 정제 과정을 통해 제조된 자연의 선택의 과정을 거친 화합물이므로, Puromycin을 대체할 수 있는 획기적인 물질로 볼 수 있다. Cystocin, a derivative of Puromycin, is a new material derived from Streptomyces sp GCA0001, new strain of Actinomycetes spiecies.This compound has outstanding biological activities in anti-bacteria, anti-tumor and anti-virus than former Puromycin compounds.And it is chosen by natural selection processing through extraction, isolation and purification from, so it may replace old Puromycins in most applications.
( Hei Chan Lee ),( Seung Don Lee ),( Jae Kyung Sohng ),( Kwang Kyoung Liou ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.4
Glucose-l-phosphate uridylyltransferase from E. coli K12 was used to convert uridine-5`-triphosphate and glucose-l-phosphate to UDP-D-glucose. The conversion was efficient and completed within 5 minutes under the employed conditions. In addition, thymidine-5`-monophosphate kinase and acetate kinase were proven to be non-specific, converting udridine-5`-monophosphate to uridine-5`-triphosphate with 55% conversion after 6 h, which was much slower than the production of TTP under the same conditions (complete conversion within one hour). Since these two reactions could proceed under the same conditions, a one-pot synthesis of UDP-D-glucose with ATP regeneration was designed from easily available starting materials, and conversion up to 40% by HPLC peak integration was achieved given a reaction time of 4 h.
Lee, Hei Chan 한국공업화학회 1996 Journal of Industrial and Engineering Chemistry Vol.2 No.2
The purpose of this research is to overcome the oxygen transfer limitations generally found in mammalian cell bioreactors, which most often limit the productivity of commercial scale processes using mammalian cells. Instead of developing techniques to provide more oxygen to the cells, we propose to develop strategies that allow the cells to grow normally while using little to no oxygen. The possibility for culturing cells in the presence of rotenone, respiration inhibitor, is discussed using the T cell lymphoblastoid, Jurkat, as a model. Respiration of Jurkat was completely inhibited at 40 nM rotenone. Jurkat cells were able to grow in rotenone containing media only if 1 mM pyruvate is added to the serum-free medium and addition of 1% serum helped Jurkat cells to grow at a nearly normal rate up to 65% of maximum cell density of rotenone-free culture, Inhibition of respiration reduced significantly glutamine consumption and the production of ammonia, a growth inhibitory by-product.