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Heeyeon BAEK,Joo Young PARK,Yong Hwan KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Lignin peroxidase is the promising enzyme spanning various industries such as cosmetics and biorefinery because of its high redox potential which can oxidize phenolic and even non-phenolic compounds. Although its high potential to industrial application, it is still challenging because of its complexity in the production, especially expression and purification. This complexity is originated from four disulfide bonds of lignin peroxidase which expressed as an insoluble form in Escherichia coli requiring unfolding and refolding in vitro. Nevertheless, removing disulfide bond by rational design has not been attempted because it is known that disulfide bond contributes to stabilize the tertiary structure of proteins. But in our previous study, we obtained the triple mutant of PcLiP01 which have incredibly high thermodynamic stability by introducing the interaction between heme and neighboring residues. In this study, we designed mutants to remove the disulfide bond of the triple mutant with site-directed mutagenesis for soluble expression of recombinant lignin peroxidase in E. coli.
Pseudo-LiDAR를 활용한 자율주행 영상인식 시스템의 3D 객체 검출 성능향상 연구
이철우(Cheolwoo Lee),백초혜(Chohye Baek),나희연(Heeyeon Nah) 한국자동차공학회 2022 한국자동차공학회 학술대회 및 전시회 Vol.2022 No.11
With the rise of artificial intelligence (AI) and the success of various deep neural network (DNN) applications, the autonomous driving has gained significant interest as one of promising research fields in both industry and academia. Autonomous driving technologies are largely divided into three stages which are perception, decision and control, and AI is making great research achievements in the perception. So far, the LiDAR sensor has been considered as a primary sensing media since it provides accurate depth information. Nevertheless, researchers have sought the alternative of the LiDAR due to the high cost and power consumption, as well as its unattractive design. In this work, we present an alternative, cost-effective and highly accurate 3D object detection mechanism built upon a simple stereo camera sensor, while providing performance comparable to the one based on LiDAR sensor. By converting images into point cloud called Pseudo-LiDAR and using it, then we can achieve 3.6x greater accuracy than image based algorithms. In terms of speed, we can achieve 18x faster inference time than MV3D or F-PointNet. In conclusion, an accurate and cost-effective object detector can be made by combining Complex-YOLO and the Pseudo-LiDAR method.
Park Ki-Sup,Kim Doo Ri,Baek Jin Yang,Shin Areum,Kim Kyung-Ran,Park Hwanhee,Son Sohee,Cho Heeyeon,Kim Yae-Jean 대한의학회 2023 Journal of Korean medical science Vol.38 No.48
Background: Pediatric urinary tract infection (UTI) caused by extended-spectrum β-lactamase (ESBL)-positive gram-negative bacilli (GNB) has limited options for oral antibiotic treatment. The purpose of this study was to investigate the susceptibility of ESBLpositive Escherichia coli and Klebsiella pneumoniae isolates from pediatric urine samples to two oral antibiotics (fosfomycin and nitrofurantoin). Methods: From November 2020 to April 2022, ESBL-positive E. coli and K. pneumoniae isolates from urine samples were collected at Samsung Medical Center, Seoul, Korea. Patients over 18 years of age or with malignancy were excluded. For repeated isolates from the same patient, only the first isolate was tested. Minimum inhibitory concentrations (MICs) were measured using agar (fosfomycin) or broth (nitrofurantoin) dilution methods. MIC50 and MIC90 were measured for fosfomycin and nitrofurantoin in both E. coli and K. pneumoniae. Results: There were 117 isolates from 117 patients, with a median age of 7 months (range, 0.0–18.5 years). Among 117 isolates, 92.3% (108/117) were E. coli and 7.7% (9/117) were K. pneumoniae. Isolates from the pediatric intensive care unit (PICU) and general ward (GW) was 11.1% (13/117) and 88.9% (104/117), respectively. Among 108 E. coli isolates, MIC50 and MIC90 for fosfomycin were 0.5 μg/mL and 2 μg/mL, respectively. Fosfomycin susceptibility rate was 97.2% (105/108) with a breakpoint of 128 μg/mL. Fosfomycin susceptibility rate was significantly lower in PICU isolates than in GW isolates (81.8% vs. 99.0%, P = 0.027). For nitrofurantoin, both the MIC50 and MIC90 were 16 μg/mL. Nitrofurantoin susceptibility rate was 96.3% (104/108) with a breakpoint of 64 μg/mL based on Clinical and Laboratory Standards Institute guidelines. Among the nine K. pneumoniae isolates, the MIC50 and MIC90 for fosfomycin was 2 μg/mL and 32 μg/mL, respectively. MIC50 and MIC90 for nitrofurantoin were 64 μg/mL and 128 μg/mL, respectively. Conclusion: For uncomplicated UTI caused by ESBL-positive GNB in Korean children, treatment with fosfomycin and nitrofurantoin for E. coli infections can be considered as an effective oral therapy option.