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형태학적 형질과 분자 표지들을 이용한 콩 유전자원의 분류
최용순,김남수,신영범,최익영,임성희,김두환 한국유전학회 2000 Genes & Genomics Vol.22 No.2
Detection and characterization of genetic diversity in germplasm are the critical steps for crop improvement. We have analyzed the genetic variation in a set of 108 different soybean accessions with agronomic traits and molecular markers, and tried to detect the association between the molecular diversity and agronomic trait variation. 10 agronomic traits including plant height, leaf shape, number of branches per plant, number of nodes per plant, number of podes per plant, 100-seed weight, days to flowering, days from flowering to maturity, days to maturity, and crude oil content showed high variation among the soybeans. Genetic correlation among traits was also sought. Plant height and 100-seed weight showed significant correlation coefficient with many other traits. In principal component analysis, 70% of the total variation were accounted by the first 4 principal components and days to maturity among ten agronomic traits took the largest proportion of the variation. The 108 soybean lines were grouped into 12 clusters at the minimum similarity of 0.815 with Simple Sequence Repeated DNA Polymorphism (SSRP) and Amplified Fragment Length Polymorphism (AFLP) markers. The clustering pattern did not permit the grouping of the soybean lines according to their geographic origins. In an attempt to associate the variation of agronomic traits with the molecular diversity, except for the number of pods per plant, the other nine agronomic traits showed highly significant differences between one or more of the molecular subclusters at the Least Significant Difference (LSD) level of 5%. Although most of the studies on the association of the agronomic traits with the molecular markers were done with the molecular maps, our attempt could provide another mean for detecting the association of the agronomic trait variation with molecular diversity.
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Resistance exercise improves cardiac function and mitochondrial efficiency in diabetic rat hearts
Ko, Tae Hee,Marquez, Jubert C.,Kim, Hyoung Kyu,Jeong, Seung Hun,Lee, SungRyul,Youm, Jae Boum,Song, In Sung,Seo, Dae Yun,Kim, Hye Jin,Won, Du Nam,Cho, Kyoung Im,Choi, Mun Gi,Rhee, Byoung Doo,Ko, Kyung Springer-Verlag 2018 Pfl ugers Arch Vol.470 No.2
<P>Metabolic disturbance and mitochondrial dysfunction are a hallmark of diabetic cardiomyopathy (DC). Resistance exercise (RE) not only enhances the condition of healthy individuals but could also improve the status of those with disease. However, the beneficial effects of RE in the prevention of DC and mitochondrial dysfunction are uncertain. Therefore, this study investigated whether RE attenuates DC by improving mitochondrial function using an in vivo rat model of diabetes. Fourteen Otsuka Long-Evans Tokushima Fatty rats were assigned to sedentary control (SC, n = 7) and RE (n = 7) groups at 28 weeks of age. Long-Evans Tokushima Otsuka rats were used as the non-diabetic control. The RE rats were trained by 20 repetitions of climbing a ladder 5 days per week. RE rats exhibited higher glucose uptake and lower lipid profiles, indicating changes in energy metabolism. RE rats significantly increased the ejection fraction and fractional shortening compared with the SC rats. Isolated mitochondria in RE rats showed increase in mitochondrial numbers, which were accompanied by higher expression of mitochondrial biogenesis proteins such as proliferator-activated receptor-gamma coactivator-1 alpha and TFAM. Moreover, RE rats reduced proton leakage and reactive oxygen species production, with higher membrane potential. These results were accompanied by higher superoxide dismutase 2 and lower uncoupling protein 2 (UCP2) and UCP3 levels in RE rats. These data suggest that RE is effective at ameliorating DC by improving mitochondrial function, which may contribute to the maintenance of diabetic cardiac contractility.</P>
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폐경이후 여성의 영양섭취 및 활동관계와 골밀도의 상관관계에 관한 연구
문수재,최은정,이명희,임승길,허갑범 연세대학교 생활과학연구소 1993 生活科學論集 Vol.7 No.-
The purpose of the present study was to examine correlations between nutrient intake, physical activity and bone mineral density in postmenopausal women. The subjects were 18 normal and 17 osteoporotic subjects. A trained nutritionist interviewed the subject about their usual dietary intake and daily activity by means of a questionnaire, Bone mineral density was measured by dual photon absorptiometry. The age distribution of the subjects was 51-70 years. The results of this study can be summerized as follows: 1. The nutrient intake of the normal subjects showed higher value than the intake of osteoporotic subjects at all age levels. Seventy percent of the normal group had taken more than 70% of the Recommended Dietary Allowance in 8 out of the 9 nutrients measured in this study. On the other hand 50% of the osteoporotic group had taken below 70% of RDA in protein, vitamin A, riboflavin and ascorbic acid. 2. The dietary sources of calcium were compared between the normal and the osteoporotic group. Plant sources provided most of the calcium in both groups, but the normal group had higher calcium intake from animal sources than the osteoporotic group. Among the total calcium intake, calcium intake from milk and milk products was very low and contributed only 10 - 13% for the normal group and 4 -5% for osteoporotic group. 3. There was a positive correlation between nutrient intake and bone mineral density. In particular, dietary calcium intake showed a highly significant positive correlation with bone mineral density of spine, femur neck, Ward's triangle and trochanteric region(p < 0.01) in subjects between the ages 51 - 60 years. 4. All the normal group subjects showed significantly higher physical activity and daily total energy expenditure than the osteoporotic group subjects. Higher physical activity was significantly associated with greater bone mineral density of four sites(p < 0.01, p < 0.05) In conclusion, physical activity as well as dietary calcium intake seemsto be important in preventing osteoporosis on postmenopausal women.
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