Silencing of long noncoding RNA PVT1 inhibits podocyte damage and apoptosis in diabetic nephropathy by upregulating FOXA1

Dong-Wei Liu,Jia-Hui Zhang,Feng-Xun Liu,Xu-Tong Wang,Shao-Kang Pan,Deng-Ke Jiang,Zi-Hao Zhao,Zhang-Suo Liu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

The number of patients with diabetic nephropathy (DN) is still on the rise worldwide, and this requires the development of new therapeutic strategies. Recent reports have highlighted genetic factors in the treatment of DN. Herein, we aimed to study the roles of long noncoding RNA (lncRNA) plasmacytoma variant translocation 1 (PVT1) and histone 3 lysine 27 trimethylation (H3K27me3) in DN. A model of DN was established by inducing diabetes in mice with streptozotocin. Mouse podocyte clone 5 (MPC5) podocytes and primary podocytes were cultured in normal and high glucose media to observe cell morphology and to quantify PVT1 expression. The roles of PVT1 and enhancer of zeste homolog 2 (EZH2) were validated via loss-of-function and gain-of-function in vitro experiments to identify the interactions among PVT1, EZH2, and forkhead box A1 (FOXA1). The podocyte damage and apoptosis due to PVT1 and FOXA1 were verified with in vivo experiments. PVT1 was highly expressed in MPC5 and primary podocytes in DN patients and in cultures grown in high glucose medium. A large number of CpG (C-phosphate-G) island sites were predicted at the FOXA1 promoter region, where PVT1 recruited EZH2 to promote the recruitment of H3K27me3. The silencing of PVT1 or the overexpression of FOXA1 relieved the damage and inhibited the apoptosis of podocytes in DN, as was evidenced by the upregulated expression of synaptopodin and podocin, higher expression of Bcl-2, and lower expression of Bax and cleaved caspase-3. The key findings of this study collectively indicate that the suppression of lncRNA PVT1 exerts inhibitory effects on podocyte damage and apoptosis via FOXA1 in DN, which is of clinical significance.

CCN4 Regulates Vascular Smooth Muscle Cell Migration and Proliferation

Hao Liu,Zhengjun Liu,Wenpeng Dong,Zhiqi Lin,Jingbo Lu,Heng Wan,Zhongxin Zhou 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.2

The migration and proliferation of vascular smooth muscle cells (VSMCs) are essential elements during the development of atherosclerosis and restenosis. An increasing number of studies have reported that extracellular matrix (ECM) proteins, including the CCN protein family, play a significant role in VSMC migration and proliferation. CCN4 is a member of the CCN protein family, which controls cell development and survival in multiple systems of the body. Here, we sought to determine whether CCN4 is involved in VSMC migration and proliferation. We examined the effect of CCN4 using rat cultured VSMCs. In cultured VSMCs, CCN4 stimulated the adhesion and migration of VSMCs in a dose-dependent manner, and this effect was blocked by an antibody for integrin 51. CCN4 expression was en-hanced by the pro-inflammatory cytokine tumor necrosis factor  (TNF-). Furthermore, knockdown of CCN4 by siRNA significantly inhibited the VSMC proliferation. CCN4 also could up-regulate the expression level of marker proteins of the VSMCs phenotype. Taken together, these results suggest that CCN4 is involved in the migration and proliferation of VSMCs. Inhibition of CCN4 may provide a promising strategy for the prevention of restenosis after vascular interventions.

Research Progress on Surface Coating Layers on the Positive Electrode for Lithium Ion Batteries

Zhen Dong Hao,Xiaolong Xu,Hao Wang,Jingbing Liu,Hui Yan 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.11

Lithium ion batteries (LIBs) are one of the most promising secondary batteries due to their advantages including long cycle life, high energy density, limited self-discharge, high operating voltage and environmental friendliness. The development of electrode materials is crucial for the further application of LIBs. There are many effective ways to enhance the performance of positive electrode materials of LIBs such as surface coating, ion doping, preparation of composite materials and nanosized materials and so forth. Among them, surface coating is considered to be a promising way to improve the electrochemical performance of LIBs. Surface coating can normally form a physical barrier or a doped surface layer to play favorable roles for the electrode materials, such as hindering side reactions between positive electrode materials and the electrolyte. In this paper, different kinds of surface coating layers will be discussed according to previous research, including carbon materials, metal oxides, metal fluorides, metal phosphates, nonmetal oxides, electrode materials coating layer, hybrid coating layer, polymer and so forth. In addition, the mechanism of these coating materials will be summarized, and the future development will be discussed in this paper.

Constituents of the Root of Anemone tomentosa

Hao-Bin Hu,Xu-Dong Zheng,Yu-Feng Jian,Jian-Xin Liu,Ji-Hua Zhu 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7

A new diterpene glycoside, tomentoside I (1), along with eleven known compounds, including the four coumarins, 4,5-dimethoxyl-7-methylcoumarin (2), 4,7-dimethoxyl-5-methylcoumarin (3), isofraxidin (4) and fraxidin (5) as well as the seven triterpenoids, oleanolic acid (6), oleanolic acid 3-O-α-L-arabinopyranoside (7), oleanolic acid 3-O-β-D-galactopyranosyl-(1→3)-β-Dglucopyranoside (8), hederagenin 3-O-α-L-arabinopyranoside (9), betulinic acid (10), 18-hydroxyursolic acid (11) and 2α,3β,23-trihydroxyurs-12-en-28-oic acid (12) were isolated from the ethanolic extract of the root of Anemone tomentosa and their chemical structures were elucidated by spectroscopic methods. The antimicrobial activities of compounds 1-12 were measured using the agar disc-diffusion method. Also, their antioxidant activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) were evaluated.

Serum Carbohydrate Antigen 19-9 as an Indicator of Liver Metastasis in Colorectal Carcinoma Cases

Dong, Hang,Tang, Jie,Li, Long-Hao,Ge, Jun,Chen, Xin,Ding, Jing,Men, Hai-Tao,Luo, Wu-Xia,Du, Yang,Li, Cong,Zhao, Feng,Chen, Ye,Cheng, Ke,Liu, Ji-Yan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Purpose: The liver is the organ to which colorectal carcinomas (CRCs) most commonly metastasize, and surgical resection has been established as the most effective and potentially curative treatment for CRC with liver metastasis (LM). Therefore, surveillance of LM is vital for improvement of prognosis of CRC patients. In this study, we aimed to explore the potential value of carbohydrate antigen 19-9 (CA 19-9), carcinoembryonic antigen (CEA), and marker enzymes in indicating LM with CRC. Methods: Three groups of eligible patients with metastatic cancers were retrospectively included: CRC patients with LM (CRC-LM) or without LM (CRC-NLM), and non-CRC patients with LM (NCRC-LM). All metastatic lesions were identified by CT or MRI. Data on characteristics of the patients, the primary site, the locations of metastasis, CA 19-9, CEA, and biochemical parameters were collected for analysis. Results: A total of 493 patients were retrospectively included. More alcohol consumption was found in CRC-LM than CRC-NLM. Some biochemical enzymes were found to be significantly higher in groups with LM than without (CRC-LM or NCRC-LM v.s CRC-NLM). Both CEA and CA 19-9 were much higher in CRC-LM than CRC-NLM or NCRC-LM. For CRC patients, CA 19-9, ${\gamma}$-glutamyl transpeptidase, CEA and alcohol consumption were identified as independent factors associated with LM. Conclusion: Our analysis suggested the CA 19-9 might be a potential valuable indicator for LM of CRC in the clinic.

Supplemental Lighting by Different Light Sources Affects Growth and Development of GraftedCucumber Plug Seedlings

Liu Chen,Hao Wei,Xuan Cai,Hai Kyoung Jeong,Xiuxia Ren,Dong Il Kang,Byoung Ryong Jeong 한국원예학회 2017 한국원예학회 학술발표요지 Vol.2017 No.10

Analysis of vibration characterization of a multi-stage planetary gear transmission system containing faults

Hao Dong,Yue Bi,Bing-Xing Ren,Zhen-Bin Liu,Yue Li 국제구조공학회 2023 Structural Engineering and Mechanics, An Int'l Jou Vol.88 No.4

In order to explore the influence of tooth root cracks on the dynamic characteristics of multi-stage planetary gear transmission systems, a concentrated parameter method was used to construct a nonlinear dynamic model of the system with 30- DOF in bending and torsion, taking into account factors such as crack depth, length, angle, error, time-varying meshing stiffness (TVMS), and damping. In the model, the energy method was used to establish a TVMS model with cracks, and the influence of cracks on the TVMS of the system was studied. By using the Runge- Kutta method to calculate the differential equations of system dynamics, a series of system vibration diagrams containing cracks were obtained, and the influence of different crack parameters on the vibration of the system was analyzed. And vibration testing experiments were conducted on the system with planetary gear cracks. The results show that when the gear contains cracks, the TVMS of the system will decrease, and as the cracks intensify, the TVMS will decrease. When cracks appear on the II-stage planetary gear, the system will experience impact effects with intervals of rotation cycles of the II-stage planetary gear. There will be obvious sidebands near the meshing frequency doubling, and the vibration trajectory of the gear will also become disordered. These situations will become more and more obvious as the degree of cracks intensifies. Through experiments, the theoretical results are in good agreement with experimental results, verifying the correctness of the theoretical model. This provides a theoretical basis for fault diagnosis and reliability research of the system.

Effect of Quality of Supplemental Light on Growth and Development of Grafted Tomato Plug Seedlings

Hao Wei,Xuan Cai,Hai Kyoung Jeong,Chen Liu,Xiuxia Ren,Dong Il Kang,Byoung Ryong Jeong 한국원예학회 2017 한국원예학회 학술발표요지 Vol.2017 No.10

PFP@PLGA/Cu12Sb4S13-mediated PTT ablates hepatocellular carcinoma by inhibiting the RAS/MAPK/MT-CO1 signaling pathway

Dong Tianxiu,Jiang Jian,Zhang Hao,Liu Hongyuan,Zou Xiaomeng,Niu Jiamei,Mao Yingxuan,Zhu Mingwei,Chen Xi,Li Zizhuo,Chen Yaodong,Shi Chunying,Yang Xiuhua 나노기술연구협의회 2021 Nano Convergence Vol.8 No.29

Hepatocellular carcinoma (HCC) is one of the most malignant tumors in the world, and patients with HCC face a poor prognosis. The conventional therapeutic strategies for HCC have undergone a challenge-riddled evolution owing to side effects and unsatisfactory efficacy. Here, aiming to provide a new method of HCC elimination, we formulated a novel multifunctional nanocapsule (PFP@PLGA/Cu 12 Sb 4 S 13 , PPCu) with applications in contrast-enhanced ultrasound imaging (CEUS) and photothermal therapy (PTT). These PPCu were successfully constructed with an average diameter of 346 nm (polydispersity index, PDI = 0.276). The reinforced contrast ratio of these PPCu was determined by CEUS, revealing their promising applications in image-guided monitoring of HCC treatment. Furthermore, the excellent photoabsorption and biocompatibility indicated by organ H&E staining indicated that PPCu meet quality expectations for use as photothermal transduction agent (PTA). PPCu treatment at 50 °C and higher temperatures efficiently repressed the proliferation, induced the apoptosis and decreased the motility of HCC cells. These effects might have been results of RAS/MAPK/MT-CO1 signaling pathway inhibition. In summary, PPCu were constructed to integrate CEUS and PTT successfully into therapy, which can lead to HCC elimination through RAS/MAPK/MT-CO1 signaling pathway repression.

Comparison of Robust Methods for Shear Wave Speed Estimation by Simulation

Hao Yin,Dong C. Liu,Xin Liu 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.8

Mechanical properties of tissue are often related to the pathological state of tissue. Therefore, non-invasively measuring tissue stiffness has important clinical applications. With the assumption of isotropy, incompressibility and linearity, the shear modulus of tissue is related to its shear wave propagation speed. Acoustic radiation force from a focused ultrasound beam can be used to generate shear waves at the focal region within tissue, which propagate orthogonally to the direction of the pushing ultrasound beam. The shear wave speed can be estimated based on the so called time-to-flight principle. The shear wave arrival time determined at several lateral positions along the shear wave propagation path can be measured by the displacement profiles, which can be tracked using correlation-based method by pulse-echo ultrasound. This approach has been successively used with various modifications by several groups. The purpose of this study is to design a simulation method to generate the pulse-echo ultrasound signal, calculate the displacement profile in the spatial and time domain, and estimate the shear wave speed using RANSAC, Radon Sum and robust linear regression method, compare and analyze the algorithm performance of these methods.