BRAF-Activated Long Noncoding RNA Modulates Papillary Thyroid Carcinoma Cell Proliferation through Regulating Thyroid Stimulating Hormone Receptor

Haitao Zheng,Meng Wang,Lixin Jiang,Haidi Chu,Jinchen Hu,Jinyao Ning,Baoyuan Li,Dong Wang,Jie Xu 대한암학회 2016 Cancer Research and Treatment Vol.48 No.2

Purpose The importance of long noncoding RNAs (lncRNAs) in tumorigenesis has recently been demonstrated. However, the role of lncRNAs in development of thyroid cancer remains largely unknown. Materials and Methods Using quantitative reverse transcription polymerase chain reaction, expression of three lncRNAs, including BRAF-activated long noncoding RNA (BANCR), papillary thyroid cancer susceptibility candidate 3 (PTCSC3), and noncoding RNA associated with mitogen-activated protein kinase pathway and growth arrest (NAMA), was investigated in the current study. Results Of the three lncRNAs (BANCR, PTCSC3, and NAMA), expression of BANCR was significantly up-regulated while PTCSC3 and NAMA were significantly down-regulated in papillary thyroid carcinoma (PTC) compared to that in normal tissue. BANCR-knockdown in a PTC-derived cell line (IHH-4) resulted in significant suppression of thyroid stimulating hormone receptor (TSHR). BANCR-knockdown also led to inhibition of cell growth and cell cycle arrest at G0/G1 phase through down-regulation of cyclin D1. In addition, BANCR was enriched by polycomb enhancer of zeste homolog 2 (EZH2), and silencing BANCR led to decreased chromatin recruitment of EZH2, which resulted significantly reduced expression of TSHR. Conclusion These findings indicate that BANCR may contribute to the tumorigenesis of PTC through regulation of cyclin D1 and TSHR.

A Study on Influence of Decisiion Control of Parent Firms on Knowledge Acquisition and Technological Innovation of IJVs

Haitao Zheng 인하대학교 정석물류통상연구원 2009 인하대학교 정석물류통상연구원 학술대회 Vol.2009 No.10

Previous research examining the effectiveness of international joint ventures (IJVs) has focused on the differences in the background of IJVs’ parent firms, while little attention has been given to IJVs themselves. This study combines control theory and the perspective of knowledge integration to develop a general model of IJVs technological innovation. Specifically, we examine how IJV and parent involvement in the strategic decision control influences the technological innovation of IJV. We analyze this model from the perspective of structural equation model with 96 manufacturing and service-oriented IJVs in China as the samples for this study. Our results indicate that decision control positively affects IJVs’ technological innovation in different ways, and knowledge acquisition plays a basic role This study sets out to contribute to the understanding of the roles of IJVs’ decision control in gaining their competitive advantage of management innovation.

Study on temperature characteristics of gasoline engine particulate filters during fuel cut-off

Nan Li,Zheng Nan,Qiushi Zhang,Haitao Liu,Lun Hua,Caihong Zhang 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.12

Based on the bench test of a 1.5 L gasoline engine equipped with a catalytic gasoline engine particulate filter(CGPF), the fuel cut-off regeneration characteristics of the next generation with small pore size GPF and the commonGPF under different soot loading and temperatures were studied. The results showed that after the fuel was cutoffduring the regeneration process, the center temperature of the sample rose exponentially, and as the regenerationtime extended, the temperature gradually became flat. The temperature distribution inside the carrier was such that theinlet temperature was low at the axial position, and the temperature near the outlet was high, the radial position showeda trend of high temperature in the middle and low temperature at the edge, and the temperature difference betweenthe edge and the center of the carrier was large. The maximum temperature inside the GPF increased with the increasein soot loading, and the maximum temperature gradient also increased. When the soot loading reached 6 g/L and thefuel cut-off regeneration was conducted at 650 oC, the maximum and maximum temperature gradient inside the GPFexceeded the tolerance limit of the GPF, and the inside of the carrier was damaged to varying degrees. Therefore, theseparameters should be controlled during regeneration.

Research and Optimization of Lateral Compressive Performance of the 3-D Printed Beetle Elytron Plate

Caiqi Zhao,Tengteng Zheng,Lijie Shang,Haitao Lan,Shuo Yang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.8

In this paper, two types of beetle elytron plates (end-trabecular beetle elytron plate (EBEP) and middle-trabecular beetle elytron plate (MBEP)) and honeycomb plate (HP) were manufactured by 3-D printed and applied to large-span spatial structure. The lateral compressive performance of the 3-D printed beetle elytron plate was investigated by lateral compression bearing capacity test and numerical analysis. The influence of number of cylinders, ratio of the radius of the cylinder to the side length of the hexagonal honeycomb core (ratio of radius-length), thickness of core layer and configuration of beetle elytron plate on the lateral compressive performance of the beetle elytron plates were studied and the optimization method for lateral compressive performance of the beetle elytron plates was proposed. The result shows that the lateral compression bearing capacity of EBEP is greater than that of MBEP, and both are greater than of HP. The lateral compression bearing capacity of the beetle elytron plate with six cylinders is about 25% higher than that of the HP without cylinders. The lateral compression bearing capacity of beetle elytron plate can be improved by increasing the thickness of plates. The results of the study will promote the application of beetle elytron plates in large-span spatial structures.

Changes of Collagen in Sea Cucumber (Stichopus japonicas) During Cooking

Xiuping Dong,Beiwei Zhu,Liming Sun,Jie Zheng,Dan Jiang,Dayong Zhou,Haitao Wu,Yoshiyuki Murata 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.4

Changes of the collagen in sea cucumber (Stichopus japonicas) during cooking were investigated. Crude collagen fibers (CCF) is more sensitive to heat than pepsin-solubilized collagen (PSC), absorbance at 226-232nm increased from 60 to 100^oC. PSC nearly completely degraded after cooking for 8-10 h, 4-6 h, 1-1.5 h, 40-50 min, and 10-20 min at 60, 70, 80, 90, and 100^oC,respectively. Collagen fiber shrinkage, disappearance of periodic cross striation, complete denaturation, and dispersion of denatured fibers at 40, 60, 80 and 100^oC,respectively, were demonstrated by transmission electron microscope (TEM). Above results might be instructional for sea cucumber processing and collagen usage.

Fe₃O₄ magnetic nanoparticles provide a novel alternative strategy for Staphylococcus aureus bone infection

Youliang, Ren,Jin, Yang,Jinghui, Zhang,Xiao, Yang,Lei, Shi,Dajing, Guo,Yuanyi, Zheng,Haitao, Ran,Zhongliang, Deng,Lei, Chu Techno-Press 2022 Advances in nano research Vol.13 No.6

Due to its biofilm formation and colonization of the osteocyte-lacuno canalicular network (OLCN), Staphylococcus aureus (S.aureus) implant-associated bone infection (SIABI) is difficult to cure thoroughly, and may occur recurrently subsequently after a long period dormant. It is essential to explore an alternative therapeutic strategy that can eradicate the pathogens in the infected foci. To address this, the polymethylmethacrylate (PMMA) bone cement and Fe3O4 nanoparticles compound cylinder were developed as implants based on their size and mechanical properties for the alternative magnetic field (AMF) induced thermal ablation, The PMMA mixed with optimized 2% Fe₃O₄ nanoparticles showed an excellent antibacterial efficacy in vitro. It was evaluated by the CFU, CT scan and histopathological staining on a rabbit 1-stage transtibial screw model. The results showed that on week 7, the CFU of infected soft tissue and implants, and the white blood cells (WBCs) of the PMMA+2% Fe₃O₄+AMF group decreased significantly from their controls (p<0.05). PMMA+2% Fe₃O₄+AMF group did not observe bone resorption, periosteal reaction, and infectious reactive bone formation by CT images. Further histopathological H&E and Gram Staining confirmed there was no obvious inflammatory cell infiltration, neither pathogens residue nor noticeably burn damage around the infected screw channel in the PMMA+2% Fe₃O₄+AMF group. Further investigation of nanoparticle distributions in bone marrow medullary and vital organs of heart, liver, spleen, lung, and kidney. There were no significantly extra Fe₃O₄ nanoparticles were observed in the medullary cavity and all vital organs either. In the current study, PMMA+2% Fe₃O₄+AMF shows promising therapeutic potential for SIABI by providing excellent mechanical support, and promising efficacy of eradicating the residual pathogenic bacteria in bone infected lesions.