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Cui, Hai-Yan,Baque, Md. Abdullahil,Lee, Eun-Jung,Paek, Kee-Yoeup 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
In an attempt to scale-up of adventitious root cultures of Echinacea angustifolia for the production of biomass and caffeic acid derivatives, i.e. echinacoside, chlorogenic acid, cichoric acid, caftaric acid, and cynarin, the effects of Murashige and Skoog (MS) medium dilutions, and initial sucrose concentrations were investigated in a 5-L airlift bioreactor. In addition, the kinetics of adventitious root growth and accumulation of secondary metabolites were also studied. The greatest root dry weight ($6.50gL^{-1}$) and accumulation of total phenolics [$22.06mgg^{-1}$ DW (dry weight)], total flavonoids ($5.77mgg^{-1}$ DW) and total caffeic acid derivatives ($10.63mgg^{-1}$ DW) were obtained at quarter-strength MS medium. Of the various gradients of sucrose tested, 5 % sucrose supplementation was regarded as an optimal concentration for enhancing productivity of biomass and bioactive compounds. Neither higher salt strength (3/4-2 MS) nor sucrose concentrations (7 and 9 %) showed promotive effect on root growth and metabolite production. The kinetic studies revealed that 4 weeks of culture period is the optimal time to achieve highest productivity of metabolites. Based on these results, a largescale (20 L) and a pilot-scale (500 L) adventitious root culture system was established. In the pilot-scale bioreactor, adventitious roots were elicitor-treated with $100{\mu}M$ methyl jasmonate (MJ) on day 28. After 1 week of elicitation, 1.75 kg dry root biomass was harvested containing $60.41mgg^{-1}$ DW of total phenolics, $16.45mgg^{-1}$ DW of total flavonoids, and $33.44mgg^{-1}$ DW of total caffeic acid derivatives. Among the caffeic acid derivatives, the accumulation of echinacoside (the major bioactive compound) in MJ-treated adventitious roots grown in the 500-L bioreactor was the highest ($12.3mgg^{-1}$ DW), which is approximately threefold more than the non-MJ-treated roots cultured in 5- and 20-L bioreactors.
Study and Application of Evaluation Hierarchy on Power Supply Capability of Distribution Network
Cui, Yan-Yan,Wei, Tao,Chen, Hai,Li, Zhi-Yong 대한전기학회 2012 The Journal of International Council on Electrical Vol.2 No.4
Against the lack of quantitative assessment method on power supply capability of distribution network, evaluation hierarchy on power supply capability of distribution network is proposed. In addition to some common indices, the hierarchy includes some new indices such as "load balancing degree of main transformers, substations and lines", "coordination of power supply between high and medium voltage network", "matching degree of power supply and transmission capability" etc. Besides that, evaluation process is put forward. In accordance with urban and rural areas, the respective adaptive scoring standards and weight values are given. The power supply capabilities of distribution network of two districts in Chongqing, China are evaluated based on the hierarchy proposed in this paper. The example proves that the method and evaluation hierarchy can not only evaluate the power supply of distribution network scientifically, find the weak link, but also guide the planning and construction of network.
Hai-Yan Cui,Md. Abdullahil Baque,Eun Jung Lee,백기엽 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
In an attempt to scale-up of adventitious rootcultures of Echinacea angustifolia for the production ofbiomass and caffeic acid derivatives, i.e. echinacoside,chlorogenic acid, cichoric acid, caftaric acid, and cynarin,the effects of Murashige and Skoog (MS) medium dilutions,and initial sucrose concentrations were investigated in a 5-Lairlift bioreactor. In addition, the kinetics of adventitiousroot growth and accumulation of secondary metaboliteswere also studied. The greatest root dry weight (6.50 g L-l)and accumulation of total phenolics [22.06 mg g-1 DW(dry weight)], total flavonoids (5.77 mg g-1 DW) and totalcaffeic acid derivatives (10.63 mg g-1 DW) were obtainedat quarter-strength MS medium. Of the various gradients ofsucrose tested, 5 % sucrose supplementation was regardedas an optimal concentration for enhancing productivity ofbiomass and bioactive compounds. Neither higher saltstrength (3/4–2 MS) nor sucrose concentrations (7 and 9 %)showed promotive effect on root growth and metaboliteproduction. The kinetic studies revealed that 4 weeks ofculture period is the optimal time to achieve highestproductivity of metabolites. Based on these results, a largescale(20 L) and a pilot-scale (500 L) adventitious rootculture system was established. In the pilot-scale bioreactor,adventitious roots were elicitor-treated with 100 lMmethyljasmonate (MJ) on day 28. After 1 week of elicitation,1.75 kg dry root biomass was harvested containing60.41 mg g-1 DWof total phenolics, 16.45 mg g-1 DWoftotal flavonoids, and 33.44 mg g-1 DW of total caffeic acidderivatives. Among the caffeic acid derivatives, the accumulationof echinacoside (the major bioactive compound)in MJ-treated adventitious roots grown in the 500-L bioreactorwas the highest (12.3 mg g-1 DW), which isapproximately threefold more than the non-MJ-treated rootscultured in 5- and 20-L bioreactors.
Hai-Yan Cui,Hosakatte Niranjana Murthy,모상현,Yong-Yi Cui,백기엽 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.1
Dendrobium candidum is an important medicinal plant rich in polysaccharides, phenols, flavonoids and its bioactive compounds are known for various pharmacological activities. Natural populations of D. candidum are depleting due to overexploitation and increasing demand for medicinal and health products. An efficient protocol for protocorm suspension cultures of Dendrobium candidum in shake flasks was developed and the effect of auxins, basal media, nitrogen concentration, sucrose concentration, organic supplements like banana homogenate, coconut water and potato extract were investigated for the production of biomass and bioactive compounds. For the production of polysaccharides, polyphenolics, and flavonoids, the optimized culture conditions were found to be MS basal medium supplemented with 0.5 mg․L-1 NAA, 5:25 mM NH4:NO3 as nitrogen source, 2.5% (w/v) sucrose, and 1% (v/v) banana homogenate. Results of the current study could be used for scale-up process.