The Viability Change of Yeast Cell Responding to Trehalose Accumulation and Maintaining Neutral Trehalase Activity under Extracellular pH Acidified by H₂SO₄

Haesun Yun,Sangkyoo Paik,Ilsup Kim,Ho-Yong Sohn,Ingnyol Jin 한국생명과학회 2002 Journal of Life Science Vol.12 No.2

Saccharomyces cerevisiae KNU5377 (KNU5377) and S. cerevisiae ATCC24858 (ATCC24858) were exposed to H₂SO₄ as a stress, which was added at various conto a YPD media. The growth of KNU5377 was reduced to approximately 60% in the YPD media containing 40 mM sulfuric acid when compared to the non-stressed condition. When their growth was monitored during an overculture, two strains, KNU5377 and ATCC24858, could not grow when exposed to over 50 mM of sulfuric acid. After a short exposure to this acid for 1 h, KNU5377 exhibited stronger resistance against H₂SO₄ than ATCC24858. The neutral trehalase activity of KNU5377 unchanged despite under various concentrations of H₂SO₄. In contrast, that of ATCC24858 was much low at higher H2SO4 concentrations. Trehalose, a non-reducing disaccharide, was maximally accumulated after a short exposure to 60 mM H₂SO₄ for KNU5377, but it was reduced under more severe stressful conditions. These results suggest that KNU5377 should modulate the trehalose concentrations under the severe stress condition of high sulfuric acid concentrations. The most highly induced protein in the KNU5377 exposed to sulfuric acid was found to be an approximately 23 kDa protein, which was revealed to be the 60S large subunit ribosomal protein, LB, by FAST A search results.

대학생의 에너지드링크 섭취실태 및 영향 요인 분석

(Haesun Yun),(Su Hee Kim),오류제보하기 테스트(Chung Yul Lee) 한국보건교육건강증진학회 2013 보건교육건강증진학회지 Vol.30 No.3

Association between Subjective Health Perception and Cohabitation Status of the Elderly

YUN HAESUN (사)위기관리이론과실천 2023 Journal of Safety and Crisis Management Vol.13 No.9

The purpose of this research is for verifying the association between subjective health status and cohabitation status of elderly group in Korea. the 8th National Health and Nutrition Examination Survey (KNHANES Ⅷ-2) raw data used for analysis. The respondents of this research were 2,314 people aged 60 and older. Data analysis was performed by SPSS. Chi-square analysis was performed for differences of subjective health perception and general characteristics. Logistic regression analysis was performed for to analyze factors related to subjective health perception and cohabitation status. In 60s age group, gender, household income, education level, cohabitation status, drinking were related to subjective health perception. In 70s, gender, education level, walking were related to subjective health perception. In 80 and older, household income, walking were related to subjective health perception, In logistic regression analysis, subjective health perception in 60s age group was 2.1 times higher in the case of cohabitation (yes) compared to cohabitation (no). Subjective health status have been related to successful aging. This research' result would be used for basic data for the developing an intervention program to improve subjective health perception in the elderly.

욕창예방을 위한 중재와 영향요인 : 체계적 문헌고찰

YUN HAESUN,박지연 한국디지털정책학회 2020 디지털융복합연구 Vol.18 No.2

This study was to perform a systematic review to examine pressure ulcer preventive intervention and risk factors. PRISMA flow diagram in accordance with the selection process of this research was done. The literatures were retrieved from domestic and international study from 2010 to June, 2019. A total of 30 literatures were selected according to the selection criteria of this study. The literatures were searched electronically using the search engine with the key words of ‘pressure ulcers’, ‘bedsore’, ‘decubitus ulcers’, ‘intervention’, ‘prevention’. The results of this study suggest that mediation with position change and pressure reduction device reduced the incidence of pressure sores and was important for prevention. Based on the results of this study, we provide basic data for preventive intervention of pressure ulcers that are appropriate for each medical institution. 본 연구의 목적은 욕창에 대한 예방적 중재방법과 영향요인을 비교 ,분석하기 위해 기존문헌을 체계적으로 고찰하였으며 논문은 PRISMA(Preferred reporting items for systematic reviews) 지침에 따라 자료를 수집하고 분석하였다. 문헌은 2010년부터 2019년 6월까지 국내외에서 발표된 논문을 대상으로 하였다. 본 연구의 논문 선정기준에 따라 총 30의 논문이 선택되었으며 실험연구가 8편, 코호트 연구가 3편, 단면조사연구가 19편 이었다. 논문검색어는 국내논문의 경우 욕창, 압박욕창, 압박궤양, 예방, 중재의 단어를 조합하여 검색하였다. 분석결과 욕창의 예방적 중재와 예측요인을 통합적으로 제시하였으며 특히 체위변경과 압력 감소 장치 적용한 중재는 욕창 발생률을 감소시켰으며 욕창발생 감소와 욕창예방에 중요한 중재임이 확인되었다. 본 연구결과를 토대로 각 의료기관에 적합한 욕창의 예방적 중재를 위한 기초 자료를 제공하고자 한다.

우리나라 성인의 당뇨병과 음주행태 관련성 분석: 제7기(2017년) 국민건강영양조사 활용

YUN HAESUN 사단법인 인문사회과학기술융합학회 2019 예술인문사회융합멀티미디어논문지 Vol.9 No.11

The purpose of this study was to investigate the relationship between Diabetes mellitus and drinking behaviors on Koreans over 30 years old. A secondary data analysis was conducted using the data of The Korea National Health and Nutrition Examination Survey, 2017. Hierarchical logistic regression and χ2 and T-test were conducted based on complex sample analysis by using SPSS/WIN 25.0 program. The study sample comprised 5,462 who was responded to the dependent variable. As a result of this study, the incidence of diabetes in amount of alcohol consumed '5-6 cups / day' was about 1.7 times higher than 1-2 cups/day’ ones in final regression analysis model. And the incidence of diabetes in binge drinking once a month was about 1.4 times higher than no binge drinking. Conclusions: These findings suggest that education and interventions are needed to correct the drinking problems in diabetics patients and to increase their sensitivity to drinking problems. The limitation of this study is that it is difficult to understand the causal relationship between diabetes and alcohol consumption. The significance of this study is representative of the results of the study by analyzing the national health and nutrition survey data, a nationwide health and nutrition survey. 본 연구의 목적은 우리나라 성인 당뇨병과 음주행태의 관련성을 파악하여 음주 행태에 따라 요구되는 맞춤형 교육과 행태변화를 위한 중재를 개발하는데 기초자료로 활용되기 위함이다. 본 연구는 2017년도 국민건강영양조사결과를 기초자료로 한 이차자료 분석연구이며, 우리나라 만 30세 이상의 성인 5,727명을 대상으로 하였다. 자료 분석은 IBM 25 ver. SPSS를 이용하였으며 복합표본을 이용하여 로지스틱 회귀분석을 실시하였다. 본 연구 결과 한 번에 마시는 음주량이 ‘5-6잔/일’ 인 경우, 음주량이 ‘1-2잔/일’에 비해 당뇨병 발생이 약 1.7배 높았으며 폭음빈도의 경우, 폭음을 안 하는 경우에 비해 ‘한 달에 한번 정도 폭음’ 하는 경우가 당뇨병 발생이 약 1.4배 높은 것으로 분석되었다. 연구결과를 토대로 당뇨병 환자의 음주문제에 대한 인식을 교정하고 음주문제에 대한 민감성을 증진할 수 있는 교육과 중재 프로그램개발이 필요하다. 본 연구는 이차자료 분석연구로 당뇨병과 음주와의 인과관계를 파악하기 어려운 제한점이 있으나 전국적 규모의 건강 및 영양조사인 국민건강영양조사 자료를 이용하여 분석함으로써 연구결과의 대표성을 가지며, 음주행태를 중심으로 분석하였다는데 의의가 있다.

Factors associated with problematic alcohol consumption in adolescents

Haesun Yun 한국보건교육건강증진학회 2017 보건교육건강증진학회지 Vol.34 No.1

Factors associated with problematic alcohol consumption in adolescents

YUN HAESUN 한국보건교육건강증진학회 2017 보건교육건강증진학회지 Vol.34 No.1

Objectives: The purpose of this study was to investigate factors that influence problematic drinking among adolescents in South Korea. The first aim of this study was to examined the problematic drinking status according to ocio-demographics characteristics, mental-health, and health behavior factors. The second aim was to identify factors affecting problematic drinking. Methods: Using statistics from the 11 th (2015) Korea Youth Risk Behavior Web-based Survey, any variations among the subjects were presented by percentages and analyzed by χ 2 -tests, and then logistic regression analysis was conducted. Results: In the final model, gender, economic status, weekly allowance, father’s education level, experience of sadness, smoking, and consumption of high-caffeine drinks were all significantly associated with problematic drinking. Conclusions: Awareness of teenagers regarding the potential health effects of problematic drinking, in particular in regards to mixing smoking and high-caffeine drinks with consumption of alcoholic beverages, should be increased through health education. Teenagers need to be taught the physical and mental dangers of drinking. A program that can teach proper drinking habits and prevent excessive drinking is needed. It should be a national matter just like the smoking policy.

High prevalence of HBV and HCV infection among intravenous drug users in Korea

Yun, Haesun,Kim, Daijin,Kim, Soyeon,Kang, Sujin,Jeong, Seunghee,Cheon, Yonghoon,Joe, Keunho,Gwon, Do-Hoon,Cho, Sung-Nam,Jee, Youngmee Wiley Subscription Services, Inc., A Wiley Company 2008 Journal of medical virology Vol.80 No.9

<P>Although intravenous drug users are a well-known route of hepatitis C virus (HCV) and hepatitis B virus (HBV) transmission, there is no data on the prevalence of HBV and HCV infection among intravenous drug users in Korea. In order to describe the prevalence of HBV and HCV infection, and to determine HCV genotypes in the population, serum samples were collected from 107 intravenous drug users during 2005–2006. Fifty-seven percent (n = 61) were HCV RNA positive and 51% (n = 55) were HBV DNA positive. Co-infection of HBV and HCV were found in 23% (n = 25). HCV genotypes 1b, 2a/2c, 2, 2b, and 3a were found in 38% (n = 23), 44% (n = 27), 8% (n = 5), 2% (n = 1), and 3% (n = 2), respectively. Moreover, mixed infections of genotypes 1b and 2a/2c were found in 5% (n = 3). When the number of patients with HCV genotype 1b compared with that of patients with genotype 2a/2c, HBV DNA titer was not significantly different by independent t-test (t = −0.881, P = 0.392 > 0.05) between the two patient groups. These results suggest that the prevalence of HBV and HCV infection among intravenous drug users is high showing over 50% in Korea and a strategic prevention program should be performed in this group to prevent further infection into local community. J. Med. Virol. 80:1570–1575, 2008. © 2008 Wiley-Liss, Inc.</P>

The complete genome sequence and molecular analysis of human hepatitis E virus genotype IV identified from a Korean patient

Yun, Haesun,Kim, Jin Seon,Lee, Hyeok Jin,Jeong, Sook Hyang,Kim, Jeong Sim,Park, Sang Jong,Hwang, Seong Gyu,Kang, Sook Kyoung,Jee, Youngmee,Kim, Jong Hyun Springer-Verlag 2010 Archives of virology Vol.155 No.6

노인요양기관의 욕창발생 위험요인과 욕창예방 관리: 혼합연구

윤혜선(Haesun Yun),박지연(Jeeyeon Park) 한국산학기술학회 2020 한국산학기술학회논문지 Vol.21 No.3

본 연구의 목적은 노인요양기관의 욕창발생 위험요인과 욕창예방 관리에 대한 내용을 비교. 분석하여 노인요양기관에 적합한 욕창예방 관리 방법을 위한 기초자료를 제공하고자 하였다. 본 연구는 체계적 문헌고찰과 포커스그룹 인터뷰를 활용한 혼합연구로 체계적 문헌고찰은 PRISMA(Preferred reporting items for systematic reviews) 지침에 따라 자료를 수집하고 분석하였으며, 포커스그룹 인터뷰는 노인요양기관에서 3년 이상 근무한 경력직 간호사를 대상으로 시행하였다. 쳬계적 문헌고찰은 2010년부터 2019년 6월까지 국내외에서 발표된 논문을 본 연구의 논문 선정기준에 따라 총 8편 논문이 선택하였다. 논문검색어는 ‘pressure ulcers’, ‘bedsore’, ‘decubitus ulcers’, ‘intervention’, ‘prevention’, ‘elderly’, ‘long term’ 단어를 조합하여 검색하였다. 포커스그룹 인터뷰는 노인요양기관에 3년 이상 근무한 간호사를 대상으로 반 구조화된 설문지를 통해 내용분석을 하였다. 연구결과 노인요양기관의 경우 특히 체위변경과 압력 감소 장치를 적용한 중재가 욕창 예방관리에 중요한 중재임이 확인되었고 기관마다 표준화된 욕창간호 관리프로토콜 및 프로그램의 활용이 필요하다고 제시되었다. 본 연구는 혼합연구방법을 통해 욕창 예방관리 방법을 통합적으로 제시하였으며 특히 다학제간 협력이 노인요양기관의 중요한 이슈임이 확인되었다. The purpose of this study was to compare the risk factors of pressure sores and preventive intervention strategies and to provide basic data for the management of pressure sores prevention for long-term care facilities. It was a mixed method study using a systematic literature review and focus group interviews for analysis. A PRISMA flow diagram was prepared in accordance with the research selection process for the systemic literature review. The studies were retrieved from domestic and international studies from 2010 to June, 2019. A total of 8 studies were selected according to the selection criteria. The studies were searched electronically using a search engine with the key words of "pressure ulcers", "bedsore", "decubitus ulcers", "intervention", "prevention", "elderly", and "long term". Focus group interviews were conducted through a semi-structured questionnaire for nurses who had worked for more than three years in long-care facilities. The study results suggest that position change and pressure reduction device reduced the incidence of pressure sores and were important for prevention. The use of standardized care protocols is necessary. Multidisciplinary cooperation was an important issue.