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충청남도 해안지역의 비브리오 패혈증균 분포 및 분리주의 특성 분석
이현아(Hyunah Lee),고영은(Young-Eun Ko),최지혜(Jihye Choi),이다연(Dayeon Lee),여성순(Seoungsoon Yeo),박종진(Jongjin Park),이미영(Miyoung Lee),최진하(Jinha Choi),박준혁(Junhyuk Park) 한국환경보건학회 2021 한국환경보건학회지 Vol.47 No.5
Background: Vibrio vulnificus has been frequently detected in seawater, fish, and shellfish mainly in the coastal areas of Chungcheongnam-do Province. Objectives: This study was conducted to investigate the analyzed biochemical properties, genetic characteristics, and distribution of Vibrio vulnificus isolated from environmental sources in coastal areas of Chungcheongnam-do Province from 2019 to 2020. Methods: A total of 1,510 samples were obtained from six different sites in Chungcheongnam-do Province. Isolated strains from the samples were identified by a VITEK 2 system and MALDI-TOF. Antibiotic susceptibility testing for 85 isolates was done by microdilution minimum inhibitory concentration methods, and 11 isolates were analyzed for 16s rRNA sequences in multiple alignments. Results: Among the 1,510 samples taken during the investigation period, 306 strains were isolated and the detection rate of V. vulnificus was 20.3%. One hundred eighty-eight strains (24.6%) from seawater and 118 strains (15.8%) from mud flats were isolated. It was mainly detected in July (17.3%), August (36.5%), and September (28.8%), and the proportion was 82.0%. Based on the CLSI-recommended breakpoints, V. vulnificus isolates were all susceptible to amoxicillin/clavulanic acid, amikacin, gentamicin, ciprofloxacin and trimethoprim/sulfamethoxazole. However, nonsusceptible isolates to ampicillin, ampicillin/sulbactam, cefazolin, cefoxitin, imipenem, tetracycline and chloramphenicol were identified. In the analysis of the nucleotide sequences for 16s rRNA of V. vulnificus isolates, it was confirmed that mutations frequently occurred between nucleotide number 922 and 952, and 98.2% to 100% nucleotide identities between isolates was verified. Conclusions: The results of this study can be used as a basic data for infection control and prevention of Vibrio vulnificus infection by describing the distribution and characteristics of Vibrio vulnificus strains isolated in coastal areas of Chungcheongnam-do Province.
An Norovirus Outbreak at a Local Festival in Chungnam Korea
Hyunah Lee,Donguk Kim,Seongmin Park,Jongjin Park,Hae-Sung Nam,Jinha Choi,박준혁 대한미생물학회 2019 Journal of Bacteriology and Virology Vol.49 No.2
Noroviruses (NoV) are the major viral pathogen causing epidemic acute gastroenteritis and outbreaks of foodborne and waterborne illness. During the local festival in Chungnam province, group food poisoning occurred outbreak by NoV infections in Jan 2019. In this study, epidemiological analysis and molecular characterization were conducted such as genotyping, phylogeny. The prevalent genotypes of food poisoning events were NoV GII.3 and GII.17, and NoV GII.3 and GII.17 isolates of this study were completely matched in nucleotide sequence comparison of capsid gene region, respectively. In underground water and stream water, various multiple genotypes of noroviruses were detected including NoV GII.3, GII.8 and GI.4 in aquatic environment of the local festival site. Among 32 worker samples, various NoVs of five genotypes (GI.7, GI.8, GII.3, GII.8, GII.17) were detected in 12 samples and expected to causing NoV contaminated by exposure to groundwater. NoV genotype GII.3, which was detected from groundwater 2, was completely consistent with that of patients and workers. Therefore, groundwater within the local festival site could be main cause of food poisoning event. Because NoV outbreaks are caused by fecal to oral transmission, proper management of sewage purification facilities, groundwater and sanitary toilets is required for many visitors, and efforts are needed to maintain clean environment.
Lee, Junghee,Hwang, Yu Jin,Kim, Yunha,Lee, Min Young,Hyeon, Seung Jae,Lee, Soojin,Kim, Dong Hyun,Jang, Sung Jae,Im, Hyoenjoo,Min, Sun-Joon,Choo, Hyunah,Pae, Ae Nim,Kim, Dong Jin,Cho, Kyung Sang,Kowall Springer Verlag 2017 Acta neuropathologica Vol.134 No.5
<P>Huntington's disease (HD) is an autosomal-dominant inherited neurological disorder caused by expanded CAG repeats in exon 1 of the Huntingtin (HTT) gene. Altered histone modifications and epigenetic mechanisms are closely associated with HD suggesting that transcriptional repression may play a pathogenic role. Epigenetic compounds have significant therapeutic effects in cellular and animal models of HD, but they have not been successful in clinical trials. Herein, we report that dSETDB1/ESET, a histone methyltransferase (HMT), is a mediator of mutant HTT-induced degeneration in a fly HD model. We found that nogalamycin, an anthracycline antibiotic and a chromatin remodeling drug, reduces trimethylated histone H3K9 (H3K9me3) levels and pericentromeric heterochromatin condensation by reducing the expression of Setdb1/Eset. H3K9me3-specific ChIP-on-ChIP analysis identified that the H3K9me3-enriched epigenome signatures of multiple neuronal pathways including Egr1, Fos, Ezh1, and Arc are deregulated in HD transgenic (R6/2) mice. Nogalamycin modulated the expression of the H3K9me3-landscaped epigenome in medium spiny neurons and reduced mutant HTT nuclear inclusion formation. Moreover, nogalamycin slowed neuropathological progression, preserved motor function, and extended the life span of R6/2 mice. Together, our results indicate that modulation of SETDB1/ESET and H3K9me3-dependent heterochromatin plasticity is responsible for the neuroprotective effects of nogalamycin in HD and that small compounds targeting dysfunctional histone modification and epigenetic modification by SETDB1/ESET may be a rational therapeutic strategy in HD.</P>
Hyunah Park,Joonhwan Lee,Sangsuk Lee 한국HCI학회 2017 한국HCI학회 학술대회 Vol.2017 No.2
Mobile remote presence (MRP) robots allow physically remote people to interact and communicate as if they are in the same place. MRP function as embodied proxy agents. In this study, we tried to examine the effect of MRP robots on quality of communication and life satisfaction for institutionalized elderly people. They usually have a mild cognitive impairment, which decreases their ability to communicate with people and will to live lively. We assumed that communicating via MRP robots would improve their quality of communication and life satisfaction through the sense of presence and mobility. To test this assumption, we conducted two experiments-involving 1) three communication conditions (telephone, video call, and MRP) to examine quality of communication, and 2) six tasks to test the perception of a presence and life satisfaction. Our results showed that quality of communication was highest in the MRP condition for most of the participants. Also, interacting with the MRP significantly increased life satisfaction, although no significant effect was found on perceived presence.
Lee, Hyunah,Shahbaz, Hafiz Muhammad,Ha, Namho,Kim, Jeong Un,Lee, Sang Jun,Park, Jiyong The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.1
Background: Korean ginseng (Panax ginseng Meyer) powder is in rising demand because powder forms of foods are convenient to handle and are highly preservable. However, ginseng powder (GP) manufactured using the conventional process of air drying and dry milling suffers nutrient destruction and a lack of microbiological safety. The objective of this study was to prepare GP using a novel process comprised of UV-TiO<sub>2</sub> photocatalysis (UVTP) as a prewashing step, wet grinding, high hydrostatic pressure (HHP), and freeze-drying treatments. Methods: The effects of UVTP and HHP treatments on the microbial population, ginsenoside concentration, and physiological characteristics of GP were evaluated. Results: When UVTP for 10 min and HHP at 600 MPa for 5 min were combined, initial 4.95 log CFU/g-fw counts of total aerobes in fresh ginseng were reduced to lower than the detection limit. The levels of 7 major ginsenosides in UVTP-HHP-treated GP were significantly higher than in untreated control samples. Stronger inhibitory effects against inflammatory mediator production and antioxidant activity were observed in UVTP-HHP-treated GP than in untreated samples. There were also no significant differences in CIELAB color values of UVTP-HHP-treated GP compared with untreated control samples. Conclusion: Combined processing of UVTP and HHP increased ginsenoside levels and enhanced the microbiological safety and physiological activity of GP.
Hyunah Kim,Seung-Hwan Yun,Seon-Woo Lee,Hyun-Na Koo,Gil-Hah Kim 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
A major concern in exporting agricultural commodities is the introduction or spread of exotic quarantine pests to the new area. To prevent spread of insect pests, various phytosanitary measures are used. In this study, we compared the effect of electron beam and X-ray irradiation on development and reproduction of the two-spotted spider mite, Tetranychus urticae. Eggs, nymphs, and adults were irradiated at target doses of 50, 70, 100, 150, 200, 250, and 300 Gy with electron beam and 10, 20, 30, 50, 70, 100, 150, and 200 with X-ray irradiation. When eggs were irradiated, egg hatching was completely inhibited at 150 Gy of electron beam and 50 Gy of X-ray irradiation. When irradiated to the nymphs, emergence was not affected at all target dose in both electron beam and X-ray irradiation. Hatchability of F1 generation was significantly decreased at 100 Gy of electron beam and 70 Gy of X-ray irradiation. When irradiated to the adults, longevity did not show any differences at all target dose in both electron beam and X-ray irradiation. These results suggest that the effect of X-ray irradiation was more potential than electron beam irradiation.