Bacillus subtilis를 이용한 발효 감자 칩의 감자 품종에 따른 아크릴아마이드 저감화 비교

여성순(Seoungsoon Yeo),임상은(Sangeun Yim),진용익(Yong-Ik Jin),장동칠(Dong-Chil Chang),장윤혁(Yoon Hyuk Chang),이영승(Youngseung Lee),정윤화(Yoonhwa Jeong),김미숙(Misook Kim) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.6

본 연구에서는 240일간 저장한 네 가지 품종의 감자를 Bacillus subtilis로 발효한 후 제조한 감자 칩의 아크릴아마이드 함량과 색도 변화를 알아보고자 하였다. B. subtilis에 의한 단시간 발효는 모든 품종의 감자 슬라이스로부터 유리된 환원당 제거에 효과적이었으나 총당 함량 감소에는 영향을 미치지는 않았다. 또한, 새봉과 진선 감자 슬라이스 내 존재하는 아스파라긴 함량도 감소하였다. 이러한 결과는 복합적으로 감자 칩의 아크릴아마이드 생성량과 색 형성에 영향을 미쳤다. 대서를 제외한 나머지 세 품종은 모두 83% 이상의 아크릴아마이드 감소율을 보였다. 따라서 본 연구는 B. subtilis에 의한 단시간 발효를 통하여 저장감자를 가공식품으로 활용할 수 있는 방안을 제시하고 있다. Acrylamide is a probable human carcinogen and can be formed during frying of starchy foods. The objective of this study was to investigate the effects of acrylamide reduction in storage potato chips by fermentation of Bacillus subtilis and compare its usefulness with four different cultivars of storage potatoes (Goeun, Atlantic, Saebong, and Jinsun). Potato slices were fermented by B. subtilis at 37°C for 0, 2, and 4 h and fried at 180°C for 115 s. The total sugar contents of the fermented potato slices did not change compared to the control. However, reducing sugar contents increased in the fermentation solution containing potato slices. Asparagine contents of Saebong and Jinsun potato slices decreased with fermentation time. Color values of the fermented potato chips were improved compared to those of non-fermented potato chips. The highest L* value was found in Saebong (57.4), followed by Goeun (56.7), Jinsun (52.5), and Atlantic (48.8) after 4 h of fermentation. Potatoes stored for 240 days generated considerable amounts of acrylamide, ranging from 4.99 to 10.38 ppm, after frying. Four hours of fermentation reduced acrylamide formation in all potato chips. The lowest acrylamide content was found in Saebong (0.77 ppm), followed by Jinsun (1.21 ppm), Goeun (1.76 ppm), and Atlantic (4.09 ppm). In conclusion, fermentation of storage potatoes by B. subtilis can effectively lower acrylamide formation during frying of potato chips.

마이크로웨이브 진공건조로 생산된 마늘, 고추 분말의 품질특성

김다인(Dain Kim),여성순(Seoungsoon Yeo),신의철(Euicheol Shin),송혁환(Hyukhwan Song),김기화(Kihwa Kim),이형용(Hyoungyong Lee),정윤화(Yoonhwa Jeong),김미숙(Misook Kim),이영승(Youngseung Lee) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.10

이 연구는 열풍, 동결, 마이크로웨이브 진공 건조방법으로 생산한 마늘, 고추 분말의 품질을 비교하고 특성을 알아보는 것이 목적이었다. 일반성분에서는 건조방법에 따른 유의적인 차이를 보이지 않았다. 열풍 건조한 마늘, 고추 분말의 pH가 유의적으로 낮은 값을 보였고 동결건조 한 마늘, 고추분말의 용해도가 유의적으로 높은 값을 보였고 원물의 색상을 가장 잘 나타냈다. 수용성비타민은 마늘, 고추 분말 모두 열풍건조에서 비타민 B₁, B₂의 값이 유의적으로 낮게 나왔고 마이크로웨이브 진공건조에서 비타민 B₃, C의 값이 유의적으로 낮게 나타났다. 마늘 분말은 건조방법에 따른 무기질 함량의 차이가 없었으나 고추 분말은 동결건조 분말의 P, Mg, Mn, Cu, Zn 값이 유의적으로 낮게 나타났다. 물성은 마늘 분말에서는 열풍건조 분말의 응집성이 유의적으로 높은 값을 보였고 고추 분말에서는 마이크로웨이브 진공건조 분말의 응집성이 유의적으로 높게 나타났다. 소비자 조사결과 건조방법에 따른 분말의 외관 및 향 강도의 차이는 나타났으나 분말을 조리식품에 적용했을 때에는 소비자 기호도에 영향을 미치지 않았다. This study was conducted to compare and characterize the quality of garlic and chili pepper powders prepared by hot air drying (HD), freezing drying (FD), and microwave vacuum drying (MVD) methods. There was no significant difference in proximate analysis according to drying methods. The pH of HD dried garlic and chili pepper powder was 6.30 and 5.02 respectively, which were significantly lower than that of the MVD. The solubility of garlic powder was highest for FD, while the FD and MVD showed higher solubility of chill pepper powder. Both samples showed significantly lower values of vitamin B₁ and B₂ for HD and significantly lower values of vitamin C for MVD. There was no difference in the mineral contents of garlic powder according to the drying methods and the P, Mg, Mn, Cu, and Zn values of chili pepper powder were lowest for FD. The cohesiveness of HD powder was significantly higher in garlic powder, while the cohesiveness of MVD powder was significantly higher in chili pepper powder. Sensory evaluation showed no significant difference in characteristics when dry powder was applied to cooked food.

소스 종류를 달리한 햄 주먹밥에서의 Staphylococcus aureus 성장예측모델 개발 및 위해평가

오수진 ( Sujin Oh ),여성순 ( Seoungsoon Yeo ),김미숙 ( Misook Kim ) 대한영양사협회 2019 대한영양사협회 학술지 Vol.25 No.1

This study compared the predictive models for the growth kinetics of Staphylococcus aureus in ham rice balls. In addition, a semi-quantitative risk assessment of S. aureus on ham rice balls was conducted using FDA-iRISK 4.0. The rice was rounded with chopped ham, which was mixed with mayonnaise (SHM), soy sauce (SHS), or gochujang (SHG), and was contaminated artificially with approximately 2.5 log CFUㆍg^-1 of S. aureus. The inoculated rice balls were then stored at 7℃, 15℃, and 25℃, and the number of viable S. aureus was counted. The lag phases duration (LPD) and maximum specific growth rate (SGR) were calculated using a Baranyi model as a primary model. The growth parameters were analyzed using the polynomial equation as a function of temperature. The LPD values of S. aureus decreased with increasing temperature in SHS and SHG. On the other hand, those in SHM did not show any trend with increasing temperature. The SGR positively correlated with temperature. Equations for LPD and SGR were developed and validated using R² values, which ranged from 0.9929 to 0.9999. In addition, the total DALYs (disability adjusted life years) per year in the ham rice balls with soy sauce and gochujang was greater than mayonnaise. These results could be used to calculate the expected number of illnesses, and set the hazard management method taking the DALY value for public health into account.

여주를 첨가한 발효음료의 소화효소 억제와 항산화 활성

박수인(Suin Park),여성순(Seoungsoon Yeo),이영승(Youngseung Lee),정윤화(Yoonhwa Jeong),김미숙(Misook Kim) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.11

본 연구는 다양한 생리활성 기능을 가지고 있는 여주(Momordica charantia L.)와 유산균의 기능성을 이용하여 건강 기능성을 갖춘 발효 음료를 개발하고자 하였다. 따라서 여주에 Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus reuteri 를 각각 접종하여 여주 유산균 발효음료를 제조하고 이들의 항산화 활성 및 소화효소 억제 효과를 연구하였다. 여주 유산균 발효음료의 pH는 24시간 발효 후 감소하였으나 총산 함량은 발효시간이 진행될수록 꾸준히 증가하였다. 총 페놀함량은 발효시간에 따른 유의적인 차이가 없었으나 총 플라보노이드 함량은 48시간 발효 후 모든 군에서 유의적으로 가장 높은 값을 나타냈다. 또한, 모든 발효음료군에서 48시간 발효 후의 α-glucosidase 저해 활성은 91.24~95.05%로 높았으나 α-amylase의 저해 활성은 14.21~19.00%로 낮았다. DPPH 라디칼 소거 활성과 FRAP 활성 모두 여주 분말 첨가량에 따라 증가하였고, 이에 따라 여주 유산균 발효음료의 프로바이오틱 효과와 탄수화물 분해효소 저해 활성 및 항산화 활성이 우수함을 확인하였으며 향후 이들이 기능성 음료로서의 이용 가능성이 높을 것으로 기대된다. This study was conducted to develop Momordica charantia L. juice fermented by four Lactobacillus species such as Lactobacillus paracasei (LPA), Lactobacillus plantarum (LPL), Lactobacillus rhamnosus (LRH), and Lactobacillus reuteri (LRE) as well as to investigate their inhibitory effects against digestive enzymes and antioxidant activities. Fermentation was performed at 37°C without nutrient supplementation for 72 h. The pH and total lactic acid contents were within the ranges of 3.75∼3.96 and 5.21∼10.04% in fermented juices, respectively. The type of starter culture and fermentation time induced changes in flavonoid contents more than total phenolic contents. All juices fermented for 48 h strongly inhibited α-glucosidase activity with the percentage of inhibition ranging of 91.24∼95.05%. Antioxidant activities of all juices mostly increased after 48 h of fermentation. Our results suggest that fermented juice possesses inhibitory activity against digestive enzymes and antioxidant activity, and they can be used as health functional beverages.

충청남도 해안지역의 비브리오 패혈증균 분포 및 분리주의 특성 분석

이현아(Hyunah Lee),고영은(Young-Eun Ko),최지혜(Jihye Choi),이다연(Dayeon Lee),여성순(Seoungsoon Yeo),박종진(Jongjin Park),이미영(Miyoung Lee),최진하(Jinha Choi),박준혁(Junhyuk Park) 한국환경보건학회 2021 한국환경보건학회지 Vol.47 No.5

Background: Vibrio vulnificus has been frequently detected in seawater, fish, and shellfish mainly in the coastal areas of Chungcheongnam-do Province. Objectives: This study was conducted to investigate the analyzed biochemical properties, genetic characteristics, and distribution of Vibrio vulnificus isolated from environmental sources in coastal areas of Chungcheongnam-do Province from 2019 to 2020. Methods: A total of 1,510 samples were obtained from six different sites in Chungcheongnam-do Province. Isolated strains from the samples were identified by a VITEK 2 system and MALDI-TOF. Antibiotic susceptibility testing for 85 isolates was done by microdilution minimum inhibitory concentration methods, and 11 isolates were analyzed for 16s rRNA sequences in multiple alignments. Results: Among the 1,510 samples taken during the investigation period, 306 strains were isolated and the detection rate of V. vulnificus was 20.3%. One hundred eighty-eight strains (24.6%) from seawater and 118 strains (15.8%) from mud flats were isolated. It was mainly detected in July (17.3%), August (36.5%), and September (28.8%), and the proportion was 82.0%. Based on the CLSI-recommended breakpoints, V. vulnificus isolates were all susceptible to amoxicillin/clavulanic acid, amikacin, gentamicin, ciprofloxacin and trimethoprim/sulfamethoxazole. However, nonsusceptible isolates to ampicillin, ampicillin/sulbactam, cefazolin, cefoxitin, imipenem, tetracycline and chloramphenicol were identified. In the analysis of the nucleotide sequences for 16s rRNA of V. vulnificus isolates, it was confirmed that mutations frequently occurred between nucleotide number 922 and 952, and 98.2% to 100% nucleotide identities between isolates was verified. Conclusions: The results of this study can be used as a basic data for infection control and prevention of Vibrio vulnificus infection by describing the distribution and characteristics of Vibrio vulnificus strains isolated in coastal areas of Chungcheongnam-do Province.