Glyoxal-induced exacerbation of pruritus and dermatitis is associated with <i>staphylococcus aureus</i> colonization in the skin of a rat model of atopic dermatitis

Han, Rafael Taeho,Kim, Hye Young,Ryu, Hyun,Jang, Wooyoung,Cha, Seung Ha,Kim, Hyo Young,Lee, JaeHee,Back, Seung Keun,Kim, Hee Jin,Na, Heung Sik Elsevier 2018 Journal of dermatological science Vol.90 No.3

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Atopic dermatitis (AD) is a highly pruritic, chronic inflammatory skin disease associated with hyperreactivity to environmental triggers. Among those, outdoor air pollutants such as particulate matter (PM) have been reported to aggravate pre-existing AD. However, underlying mechanisms of air pollution-induced aggravation of AD have hardly been studied.</P> <P><B>Objective</B></P> <P>To investigate the molecular mechanisms by which glyoxal, a PM-forming organic compound, exacerbates the symptoms of AD induced by neonatal capsaicin treatment.</P> <P><B>Methods</B></P> <P>Naïve and AD rats had been exposed to either fresh air or vaporized glyoxal for 5 weeks (2 h/day and 5 days/week) since one week of age. Pruritus and dermatitis were measured every week. The skin and blood were collected and immunological traits such as Staphylococcus aureus skin colonization, production of antimicrobial peptides and immunoglobulin, and mRNA expression of inflammatory cytokines were analyzed.</P> <P><B>Results</B></P> <P>Exposure to glyoxal aggravated pruritus and dermatitis in AD rats, but did not induce any symptoms in naïve rats. Staphylococcus aureus skin colonization was increased in the skin of both naïve and AD rats. Expression of antimicrobial peptides such as LL-37 and β-defensin-2 was also increased by exposure to glyoxal in the skin of both naïve and AD rats. The mRNA expression of Th1-related cytokines was elevated on exposure to glyoxal. However, serum immunoglobulin production was not significantly changed by exposure to glyoxal.</P> <P><B>Conclusion</B></P> <P>In AD rats, exposure to glyoxal exacerbated pruritus and cutaneous inflammation, which was associated with increased colonization of <I>S. aureus</I> and subsequent immunological alterations in the skin.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Exposure to glyoxal aggravated the symptoms in AD rats, but did not induce AD in naïve rats. </LI> <LI> <I>S. aureus</I> skin colonization and subsequent expression of antimicrobial peptides were increased after exposure to glyoxal. </LI> <LI> Exposure to glyoxal elevated the production of Th1-related cytokines such as TNF-α and IFN-γ in the AD lesional skin. </LI> </UL> </P>

여성의 유방자가검진의 지식 정도

구한나,박인경,박하영,성초아,장다엘,홍은민 이화여자대학교 간호과학대학 2012 이화간호학회지 Vol.- No.46

Purpose: This study was to grasp women’s knowledge about Breast Self-examination. Methods: We collected data from 317 women who lives in certain city, Korea using structured questionnaire from 5th, August to 20th, September. The collected data was analyzed by SPSS 19.0. Results: The average score of women’ Breast Self-examination knowledge was 6.79±3.68 (of 17). There are three categories in knowledge about Breast Self-examination. Average score of category is 2.31 ± 0.71(of 4) for knowledge of breast cancer, 2.11 ± 0.57(of 5) for knowledge of breast cancer symptom, 2.37 ± 0.45(of 8) for knowledge of Breast Self-examination. Knowledge about Breast Self-examination according to general characteristics varied significantly for different age, marital status, education, and history of breast disease. Knowledge about Breast Self-examination according to characteristics related Breast examination was higher for women who received Breast Self-examination education, received Breast Self-examination education by lecture, performed Breast Self-examination, and who received Clinical Breast Examination Conclusion: People who are teenager and over sixties, their knowledge of Breast Self-examination was low. Therefore, effective Breast Self-examination education program should be provided for women who are teenager and over sixties to promote their Breast Self-examination practice regularly.

中學 科學敎育의 探究學習에 대한 評價問項 開發 (Ⅱ)

閔庚德,楊洪準,李善行,鄭遠佑,이병교,金裕漢,羅長薰 경북대학교 과학교육연구소 1985 科學敎育硏究誌 Vol.9 No.-

This study was accomplished to develope the evaluation items for inquiry learning in the 2nd grade Middle School Science for the consecutive study of the evaluation items for inquiry learning in the 1st grade Middle School Science(U-Hang Ki et al, 1984). In this study, paper and pencil test items and performance test items are made by analyzing the abilities of inquiry according to the contents and four basic experiments from each unit in the 2nd grade Middle school science. These evaluation items were applied to tke five classes of the 2nd grade of middle school to test their validity. It is desirable that performance test schuld be used for the evaluation for the abilities of inquiry which can not be evaluated by paper and pencil test. In the evaluation methods of performance test, tester evaluation, peer evaluation and self-evaluation can be applied to the science class in a multi-student class. In higher grade, however tester evaluation is more desirable than peer and self evaluation. It is found that peer evaluation and self-evaluation make possible the perfect study by feedback.

Cannabidiol Treatment for Lennox-Gastaut Syndrome at a Single Tertiary Center in South Korea

Han Na Jang(Han Na Jang),Min-Jee Kim(Min-Jee Kim),Mi-Sun Yum(Mi-Sun Yum),Tae-Sung Ko(Tae-Sung Ko) 대한소아신경학회 2022 대한소아신경학회지 Vol.31 No.1

Purpose: This study evaluated the efficacy and tolerability of cannabidiol (CBD) as an add-on therapy for childhood-onset Lennox-Gastaut syndrome (LGS). Methods: This retrospective study enrolled patients who visited the Department of Pediatric Neurology at Asan Medical Center from March 2019 to February 2022 and were treated with CBD. Electronic medical records and clinically relevant factors (including the type of epilepsy and seizures, etiology, and the number of concomitantly used anti-epileptic drugs) were reviewed. The outcome was clinical response to CBD (≥50% or <50% seizure reduction at 1, 3, and 6 months after CBD introduction and the last follow-up visit). Relevant adverse events were monitored. Results: Thirty patients were included. The median age of epilepsy onset was 5.5 years (interquartile range [IQR], 3.3 to 25.3), with a median treatment duration of CBD of 6 months (IQR, 3.3 to 7.0). Sixteen patients (53.3%) showed ≥50% seizure reduction at the last follow-up. In a univariate analysis, patients whose epilepsy commenced after 3 years of age were more likely to respond to CBD (odds ratio, 10.11; 95% confidence interval, 1.05 to 97.00; P=0.04). Adverse events were observed in 11 patients (36.6%); the most common adverse event was somnolence. Conclusion: CBD could be a treatment option for children and young adults with drug-resistant LGS with a tolerable safety profile. Age at epilepsy onset (>3 years) was associated with a favorable response to CBD treatment. Further prospective studies with larger populations are needed to evaluate the tolerability and efficacy of CBD in patients with drug-resistant epilepsy of various etiologies.

Characterization of Monoclonal Antibodies against Heavy and Light Chains of Flounder (Paralichthys olivaceus) Immunoglobulin

( Han Na Jang ),( Jong Kyu Woo ),( Young Hye Cho ),( Seo Bong Kyong ),( Sang Hoon Choi ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.3

Flounder (Paralichthys olivaceus) Immunoglobulins (Igs) were purified from the serum of mouse IgG-immunized flounder by using aflinity chromatography. Under denaturing conditions in SDS-PAGE, the flounder Igs appeared to be composed of 2 heavy (H) chains (72 and 77 kDa) and two light (L) chains (26 and 28 kDA). Monoclonal antibodies (MAbs) were produced by the fusion of myeloma cells (SP2/0) with Balb/c mouse spleen cells that were previously sensitized against affinity-purified flounder Igs. In a Western blot analysis, the produced MAbs, FIM511, FIM519, and FIM562 recognized both the 72 and 77 kDa H chains, 26 kDa, and 25 kDa L chain, respectively. Mouse antiserum against flounder Igs reacted more strongly with the L chain of 28 kDa than with 26 kDa, suggesting that the 28 kDa molecule is more immunogenic than the 26 kDa L chain molecule. In a FACS analysis, the ratios of the Ig+ cell population in the flounder head kidney and spleen cells were 49 % and 24 % , respectively. Unexpectedly, however, the ratios of the Ig+ B-like cell population in the flounder were not significantly augmented, even after the immunization of an immunogenic antigen. This suggests that the humoral immune response in fish could be considerably different from that in mammals. The produced MAbs in this study would he useful in characterizing flounder Ig+ B-like cells and in developing flounder Ig detecting an immunoassay system.

Characterization of Monoclonal Antibodies against Heavy and Light Chains of Flounder (Paralichthys olivaceus) Immunoglobulin

Jang, Han-Na,Woo, Jong-Kyu,Cho, Young-Hye,Kyong, Seo-Bong,Choi, Sang-Hoon Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.3

Flounder (Paralichthys olivaceus) Immunoglobulins (Igs) were purified from the serum of mouse IgG-immunized flounder by using affinity chromatography. Under denaturing conditions in SDS-PAGE, the flounder Igs appeared to be composed of 2 heavy (H) chains (72 and 77 kDa) and two light (L) chains (26 and 28 kDa). Monoclonal antibodies (MAbs) were produced by the fusion of myeloma cells (SP2/0) with Balb/c mouse spleen cells that were previously sensitized against affinity-purified flounder Igs. In a Western blot analysis, the produced MAbs, FIM511, FIM519, and FIM562 recognized both the 72 and 77 kDa H chains, 26 kDa, and 28 kDa L chain, respectively. Mouse antiserum against flounder Igs reacted more strongly with the L chain of 28 kDa than with 26 kDa, suggesting that the 28 kDa molecule is more immunogenic than the 26 kDa L chain molecule. In a FACS analysis, the ratios of the Ig+ cell population in the flounder head kidney and spleen cells were 49% and 24%, respectively. Unexpectedly, however, the ratios of the Ig+ B-like cell population in the flounder were not significantly augmented, even after the immunization of an immunogenic antigen. This suggests that the humoral immune response in fish could be considerably different from that in mammals. The produced MAbs in this study would be useful in characterizing flounder Ig+ B-like cells and in developing flounder Ig detecting an immunoassay system.

Preparation of Novel T-type Polyurethanes with High Thermal Stability of Second Harmonic Generation and Their Nonlinear Optical Properties

Han-Na Jang,이주연,Ga-Young Lee 대한화학회 2008 Bulletin of the Korean Chemical Society Vol.29 No.4

2,5-Di-(2'-hydroxyethoxy)benzylidenemalononitrile (3) was prepared and polymerized with 2,4-toluenediiso- cyanate and 3,3'-dimethoxy-4,4'-biphenylenediisocyanate to yield novel T-type polyurethanes 4 and 5 containing 2,5-dioxybenzylidenemalononitrile, nonlinear optical (NLO)-chromophores as part of the polymer backbones. Polyurethanes 4 and 5 were soluble in common organic solvents such as acetone and N,N-dimethylformamide and showed a thermal stability up to 250℃ with glass-transition temperatures (Tg) in the range 119-146℃. The second harmonic generation (SHG) coefficients (d33) of poled polymer films at 1064 nm fundamental wavelength were around 5.82 ´ 10-9 esu. The dipole alignment exhibited high thermal stability up to Tg, and there was no SHG decay below 140℃ due to the partial main-chain character of the polymer structure, which was acceptable for nonlinear optical device applications.

Synthesis of Novel Y-type Nonlinear Optical Polyesters with Enhanced Thermal Stability of Dipole Alignment

Han-Na Jang,이주연 대한화학회 2008 Bulletin of the Korean Chemical Society Vol.29 No.5

2,4-Di-(2'-hydroxyethoxy)benzylidenemalononitrile (3) was prepared and polymerized with terephthaloyl chloride and adipoyl chloride to yield novel Y-type polyesters 4 and 5 containing dioxybenzylidenemalononitrile groups as NLO-chromophores, which constituted parts of the polymer backbone. The resulting polymers 4 and 5 are soluble in common organic solvents such as acetone and N,N-dimethylformamide. Polymers 4 and 5 showed thermal stability up to 300℃ in thermogravimetric analysis with glass-transition temperatures obtained from differential scanning calorimetry in the range 83-94 oC. The second harmonic generation (SHG) coefficients (d33) of poled polymer films at the 1064 nm fundamental wavelength were around 6.48 ´ 10-9 esu. The dipole alignment exhibited high thermal stability even at 10℃ higher than Tg and no significant SHG decay was observed below 105℃ partially due to the main-chain character of polymer structure, which is acceptable for NLO device applications.

Synthesis and Nonlinear Optical Properties of Novel Polyester with 2,3-Dioxybenzylidenecyanoacetate

Han-Na Jang,Hyo Jin No,Ju-Yeon Lee 대한화학회 2009 Bulletin of the Korean Chemical Society Vol.30 No.3

Synthesis and Nonlinear Optical Properties of Novel Polyester with 2,3-Dioxybenzylidenecyanoacetate

Jang, Han-Na,No, Hyo-Jin,Lee, Ju-Yeon Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.3