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Distribution of (-)-Yatein in Cupressaceae Family Analysed by High Performance liquid Chromatography
Hwang, Gwi-Seo,Phuong, Nguyen-Thi,Park, Kyung-Rae,Kim, Young-Ho,Kim, Kyeong-Ho,Kang, Jong-Seong The Pharmaceutical Society of Korea 2004 Archives of Pharmacal Research Vol.27 No.1
The method for the chiral analysis of (-)-yatein was developed and the distribution of this component in the plants of three genera like Juniperus, Thuja and Chamaecyparis belonging to Cupressaceae family was examined. The chiral analysis of (-)-yatein from the plants was carried out by high performance liquid chromatography on (R,R)-Whelk-O1 column using 81 v/v% methanol as mobile phase. The yatein content in the leaves of Juniperus was the highest in compare with that of the other two genera, providing the possibility of the chemical discrimination of the plants in Juniperus from the other plants in the Cupressaceae family. In general, the yatein content in the leaves was much higher than that in the twigs. This method could be applied for the quality control of (-)-yatein in the plants belonging to Cupressaceae family.
Hwang, Gwi-Seo,Kim, Ji-Young,Chang, Tong-Shin,Jeon, Sun-Duck,So, Dhong-Su,Moon, Chang-Kiu The Pharmaceutical Society of Korea 1998 Archives of Pharmacal Research Vol.21 No.6
Brazilin [7,11b-dihydrobenz[b]indeno[1,2-d]pyran-3,6a,9,10(6H)-tetrol] inhibited thrombin-, collagen- and ADP-induced aggregation of washed rat platelets. T hrombin- and collagen-induced ATP release were also inhibited by brazilin in a concentration-dependent manner. Brazilin inhibited the formation of platelet thromboxane $A_2$ caused by thrombin, whereas it had no effect on the prostaglandin $D_2$ formation. Brazilin inhibited $^3H$-arachidonic acid liberation from membrane phospholipids of thrombin-stimulated platelets. Brazilin inhibited the rise of intracellular free calcium caused by thrombin. These results indicate that the inhibition of phospholipase ($PLA_2$) activity and [$[Ca^{2+}]_1$ elevation might be at least a part of antiplatelet mechanism of brazilin.
Distribution of (-)-Yatein in Cupressaceae Family Analysed by High Performance Liquid Chromatography
Gwi Seo Hwang,Nguyen Thi Phuong,박경래,김영호,Kyeong Ho Kim,강종성 대한약학회 2004 Archives of Pharmacal Research Vol.27 No.1
The method for the chiral analysis of (-)-yatein was developed and the distribution of this component in the plants of three genera like Juniperus, Thuja and Chamaecyparis belonging to Cupressaceae family was examined. The chiral analysis of (-)-yatein from the plants was carried out by high performance liquid chromatography on (R,R)-Whelk-O1 column using 81 v/v% methanol as mobile phase. The yatein content in the leaves of Juniperus was the highest in compare with that of the other two genera, providing the possibility of the chemical discrimination of the plants in Juniperus from the other plants in the Cupressaceae family. In general, the yatein content in the leaves was much higher than that in the twigs. This method could be applied for the quality control of (-)-yatein in the plants belonging to Cupressaceae family.
황귀서,이기선,박종형 한국환경독성학회 1999 환경독성보건학회지 Vol.14 No.3
To elucidate the preventive effect of oriental medicine Gungchi-hwadamsun (GH) on stress, we investigated the physiological change of rats which were applied immobilization stress. For immobilization stress, rats were placed in restrainer for 12 hours a day for 3 days. During application of stress, body weight of rats was measured. After sacrifice, 8 organs were taken for measurement of organ weight. Brain was sectioned into 4 parts that are Frontal Cortex, Corpus Striatum, Hypothalamus and Hippocampus. Each part was homogenated and its catecholamine and serotonin contents were measured with HPLC. In our study, stress mainly induced increase of concentration of neurotransmitters in brain, but had mild effect on other physical function of rats. GH inhibited stress induced changes of neurotransmitter content in brain.
산약(山藥)(Dioscoreae Rhizoma)으로부터 Allantoin의 분리 및 함량분석
황귀서,Hwang Gwi-Seo 대한예방한의학회 2003 대한예방한의학회지 Vol.7 No.1
It is well known that Dioscoreae Rhizoma tonifies and benefits the Spleen and Stomach, benefits the Lung and nourishes the Kidney. A major component isolated from this herb consist of dioscin, diosgenin, allantoin, starch, alanin, mucilage, choline, amylase, and glycoprotein. In this study, we aimed to measure the content of allantoin, one of major component, to determine the quality of Dioscoreae Rhizoma.