Anti-Inflammatory Effects of Graviola Stem Bark Extracts on the Testosterone-induced Benign Prostatic Hyperplasia Model in Rats

Hong Gwan Ui,Choi Minji,Chung Myung-Hee,노재열 건강기능식품미래포럼 2021 건강기능식품미래포럼 학술지 Vol.1 No.2

Pathophysiology for the development of benign prostatic hyperplasia (BPH) is not clearly known yet but it is suggested that inflammation plays a critical role. Graviola as a traditional medicine has been used for inflammatory disorders. This study aims to investigate the ameliorative effects of graviola extract against the development of BPH. BPH was induced in male Wistar rats by subcutaneous injection of testosterone propionate (TP; 5 mg/kg) once per day for 4 weeks, and graviola stem bark (GV) extract (70, 150, 300 mg/kg) or finasteride (1 mg/kg) as a positive control were administrated by gavage feeding once per day concomitantly with TP. GV extract attenuated the prostatic index (ratio prostatic tissue weight to body weight), epithelial thickness, the amounts of target molecules (dihydrotestosterone, prostate specific antigen, prostatic acid phosphatase, cyclooxygenase [COX]-2) in the sera or prostate tissues, the mRNA expression of androgen receptor (AR), 5α-reductase, COX-2, inflammatory cytokines (interleukin [IL]-6, IL-8), growth factors (insulin-like growth factor, epidermal growth factor), transforming growth factor (TGF)-β, CCL2 and CCR2, the protein expression of AR and TGF-β, and the mast cell population in prostatic tissues. Finasteride reduced all the responses like or less than those by GV extract. The data suggest that GV extract may attenuate the development of BPH through inhibiting the release of inflammatory cytokines, growth factors and COX-2 secreted/synthetized from mast cell infiltrated into stromal area of prostatic tissues.

Inflammatory mediators resulting from transglutaminase 2 expressed in mast cells contribute to the development of Parkinson's disease in a mouse model

Hong, Gwan Ui,Cho, Jin Whan,Kim, Soo Youl,Shin, Joo Ho,Ro, Jai Youl Elsevier 2018 Toxicology and applied pharmacology Vol.358 No.-

<P><B>Abstract</B></P> <P>This study aimed to investigate the role of transglutaminase 2 (TG2) expressed in mast cells in substantia nigra (SN) in Parkinson's disease (PD) model or human PD patients. C57BL/6 mice received 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) by ip injection to induce PD. Bone marrow-derived mast cells (BMMCs) were adoptively transferred to TG2 knockout (KO or TG2<SUP>−/−</SUP>) mice by iv injection 1 day before MPTP injection or stimulated by 1 methyl-4-phenylpyridinium (MMP<SUP>+</SUP>). KO-MPTP mice showed reduced expression of tyrosine hydroxylase (TH) and dopamine (DA) transporter (DAT) and loss of TH<SUP>+</SUP> DA neurons, and expression of markers (c-kit, tryptase, FcεRI), mediators' release (histamine, leukotrienes, cytokines), and TG2 related to mast cells, and co-localization of DA neuronal cells and mast cells in SN tissues or release of mediators and TG2 activity in SN tissues and sera versus those in WT (wild type)-MPTP or BM + KO-MPTP mice. KO-MPTP mice reversed the alterations of behavior. KO-BMMCs-transferred KO-MPTP (BM + KO-MPTP) mice had restoration of all the responses versus the KO-MPTP mice. MPP<SUP>+</SUP>-stimulated BMMCs had increased mediators' release, which were inhibited by TG2 inhibitor (R2 peptide). All the mediators and TG2 activity were also increased in the sera of human PD patients. The data suggest that TG2 expressed in mast cells recruited into SN tissues might contribute to neuroinflammation, which is known as one of the important features in pathogenesis of PD, via up-regulating the release of various mediators.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Mast cells recruited into substantia nigra (SN) of PD, are activated by MPP<SUP>+</SUP>. </LI> <LI> The activated mast cells activate TG2 in the SN tissues of PD model in mice. </LI> <LI> Mast cells-activated TG2 releases mediators such as histamine, LTs and cytokines. </LI> <LI> Mediators may induce neuroinflammation caused DA neuron death in mouse and human PD. </LI> <LI> TG2 inhibitor may be developed as a therapeutic agent for human PD patients. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Hwang Chan Go, a Mixture of Onion, Garlic and Wild Rice Extracts, Reduces the Release of Mediators from Mast Cell in Ovalbumin-Induced Allergic Lung in Mice

Hong Gwan Ui,Hong Eun Young,Chung Myung-Hee,노재열 건강기능식품미래포럼 2021 건강기능식품미래포럼 학술지 Vol.1 No.4

Hwang Chan Go (HCG), a mixture of extracts of Allium cepa (onion), Allium sativum (garlic) or Zizania latifolia (wild rice) was shown to have anti-allergic activities but its mechanism of action has not been elucidated yet. Thus, HCG was examined for its effects on the allergic responses in B57bl/6 mice with asthma induced with ovalbumin (OVA). HCG, when administered to the OVA-induced allergic mice, was found to reduce levels of OVA-specific IgE, infiltration of inflammatory cells including mast cells into bronchoalveolar lavage (BAL) fluid or lung tissues, bronchial goblet cell hyperplasia, expression of eosinophil major basic protein (EMBP), mucin 5ac (Muc5ac), migration molecules, cytokines and COX1/2, 5-lipoxygenase (5-LOX), c-kit and tryptase in the lung tissues, the release of granular mediator (histamine) and newly synthetized mediators (PGD2 and LTC4) and expression of inflammatory cytokines. The data suggest that HCG has a therapeutic potential for allergic asthma.

Ryupunghwan (7 Plant Extract Blend) Improves the Destructive Cartilage Through Regulating Proteases and Cytokines Secreted From Mast Cells Infiltrated Into Synovial Membranes in Collagenase II-Induced Osteoarthritis Model in Rats

Hong Gwan Ui,Chung Myung-Hee,Jai Youl Ro 건강기능식품미래포럼 2023 건강기능식품미래포럼 학술지 Vol.3 No.4

Osteoarthritis (OA) is a chronic degenerative disease characterized by synovium inflammation, cartilage destruction and bone margin erosion. Mast cells are multifunctional in the pathogenesis of OA through releasing proteases and inflammatory cytokines when the cells are activated. The seven plants has been reported to have anti-oxidant, anti-inflammatory, and anti-OA effects. Thus, a mixture of seven extracts of respective plants with the adjusted percentage ratio was prepared and named Ryupunghwan (RPH) under the assumption that the mixture has a higher efficacy on OA than the one plant extract. This study was performed to investigate the effect of RPH on OA using a collagenase II (Col II)-induced OA model in rats. To induce OA, Col II was injected in the articular space of right knee of Wistar rats. After 3 days, the rats with OA were orally administered RPH (50, 100, 300 mg/kg daily) for 3 weeks. Joint tissues were stained using Safranin-O for proteoglycan, hematoxylin-eosin for inflammatory cells and May-Grünwald-Giemza stains for mast cells. Synovial fluids were used for assays of amounts of matrix metalloproteinase (MMP) 1, MMP13, and pro-inflammatory cytokines (interleukin [IL]-1β, tumor necrosis factor [TNF]-α, IL-6) by enzyme-linked immunosorbent assay, and MMP2 and MMP9 by gelatin zymography. The OA rats revealed an increase in irregularities in the surface layer of cartilage, proteoglycan loss (red staining), collage destruction by MMP2/9. The OA rats also increased infiltration of inflammatory and mast cells into synovial membranes, amounts of MMP1, MMP9, MMP13, activities of MMP2/9, pro-inflammatory cytokines in synovial fluid, compared to normal rats. However, in OA rats with RPH administered, all these changes indicating cartilage destruction and arthritis were significantly decreased compared to the OA rats. These data suggest that RPH has anti-inflammatory effects on OA through inhibiting the secretion of MMPs and pro-inflammatory cytokines from chondrocytes and mast cells.

하악 대구치 근심치근의 근관 형성방법에 따른 각 부위별 근관벽 후경 및 근관 면적의 변화에 관한 연구

홍찬의,박종관 大韓齒科保存學會 1994 Restorative Dentistry & Endodontics Vol.19 No.1

GMP 공장과 BCR

홍관의,Hong, Gwan-Ui 한국설비기술협회 1988 설비 : 공조ㆍ냉동ㆍ위생 Vol.5 No.11

GMP설비의 구조특성

홍관의,Hong, Gwan-Ui 한국설비기술협회 1987 설비 : 공조ㆍ냉동ㆍ위생 Vol.4 No.7

크린룸의 설계

홍관의,Hong, Gwan-Ui 한국설비기술협회 1987 설비 : 공조ㆍ냉동ㆍ위생 Vol.4 No.8

비대면 시스템 구현을 위한 자율주행 서빙 로봇

홍정훈 ( Jeoun-hun Hong ),송창의 ( Chang-ui Song ),김대관 ( Dae-gwan Kim ),이근호 ( Geun-ho Lee ) 한국정보처리학회 2020 한국정보처리학회 학술대회논문집 Vol.27 No.2

최근 코로나 사태로 인하여 한국에서는 사람 간에 거리를 두는 사회적 거리 두기와 생활 속 거리 두기를 시행하고 있다. 또 식당에서의 지출 중 가장 큰 비율을 차지하고 있다. 인건비를 절감하기 위해 본 연구는 2D Lidar 센서를 사용하여 식당에서의 사람과의 접촉이 가장 큰 서빙의 역할을 로봇이 직접 하는 것을 목표로 하였다. Lidar 센서를 이용해서 SLAM을 구현하였고 안드로이드 앱을 사용하여 주문 결제를, Four Bar Linkage를 사용하여 음식 전달 부분을 구현하였다.

Effects of a Mixture of Three Extracts of Wolfberry, Chives and Graviola on the Erectile Dysfunction Induced by Bilateral Cavernous Nerve Injury in Rats

Jai Youl Ro,Gwan Ui Hong,신연호,Chung Myung-Hee 건강기능식품미래포럼 2022 건강기능식품미래포럼 학술지 Vol.2 No.4

Although some chemicals are available for erectile dysfunction (ED), efforts have been also made to seek health functional foods that can improve ED. Lycium barbarum (wolfberry) and Allium tuberosum (chives) were reported to enhance erectile function. And Annona muricata L is known to have anti-inflammatory and antioxidant actions. We assumed that if the three plants were used together, they may enhance erectile function synergistically. Hyunsung Vital Co. Ltd. prepared a mixture of water extracts of the three plants and named it Jikdaijangryeuk (JDJR). In the present study, JDJR was tested for the effect of improving ED in the rats subjected to bilateral corpus cavernous nerve injury (BCNI). BCNI decreased intra-cavernous pressure and also induced biological responses in the penile tissue that lead to ED, which were decreases in the expressions of endothelial nitric oxide (NO) synthase (eNOS) and nervous NO synthase (nNOS) that produce NO (a stimulator of 3′,5′-cyclic guanosine monophosphate [cGMP] synthesis), neurofilament-1 (a marker of nerve fibers) and an anti-apoptotic protein (Bcl2) and in the amounts of NO, cGMP (a blood vessel dilator) and smooth muscle as well as increases in the expression of inducible NO synthase (iNOS) (an inducer of microvasculature dysfunction), phosphodiesterase-5 (a cGMP destroyer), apoptotic molecules (caspase-3 and Bax) and transforming growth factor-β (a fibrosis inducer) and in the amount of asymmetric dimethylarginine (an endogenous NO synthases inhibitor). These data indicate that BCNI suppresses function of NO/cGMP axis and causes apoptosis and fibrosis of cavernous tissue. JDJR, however, reversed all these responses in a dose-dependent manner and the effects of JDJR were comparable to those of sildenafil (a positive control). The powder of Platycodon grandiflorum (a negative control) did not show the effects. These data support that JDJR has the action to enhance erectile function and thus, may be of help for ED.