Mesenchymal-to-epithelial transition of osteoblasts induced by Fam20c knockout

Geng Ya-Wei,Zhang Zhen,Jin Han,Da Jun-Long,Zhang Kai,Wang Jian-Qun,Guo Yu-Yao,Zhang Bin,Li Ying 한국유전학회 2022 Genes & Genomics Vol.44 No.2

Background: Fam20c is intimately related to tissue development and diseases. At present, it has been reported that Fam20c regulates the mineralization of osteoblasts, but there are few reports on other effects. Objective: To study the effect of Fam20c on osteoblasts by knocking out the Fam20c gene. Methods: Fam20c knockout osteoblasts were constructed by transfecting mouse osteoblasts with lentivirus. The proliferation, migration and mineralization of Fam20c knockout cells were detected by CCK-8, scratch test and alizarin red staining assays. The subcellular structure was observed by transmission electron microscopy. RT-PCR was used to detect the differential expression of mesenchymal-to-epithelial transition (MET)-related marker genes and core transcription factors. The differential expression of MET-related proteins was detected by immunofluorescence or Western blot. Transcriptome analysis of Fam20c knockout osteoblasts was performed, and real-time PCR was used to verify transcriptome analysis related to MET. Results: The proliferation ability of osteoblasts was not significantly changed after Fam20c deletion, but the migration ability and mineralization ability were significantly weakened. There were tight junctions between Fam20c knockout cells. The expression of mesenchymal cell marker genes and core transcription factors was significantly decreased, and the expression of epithelial cell marker genes was significantly increased. The expression of mesenchymal cell marker proteins was significantly decreased, and the expression of epithelial cell marker proteins was significantly increased. Multiple signalling molecules and pathways involved in MET have changed. Conclusions: Knockdown of Fam20c resulted in MET. Fam20c affects the transcription of key factors in osteoblast MET.

Limit distribution of maxima of strongly dependent Gaussian vector sequences under complete and incomplete samples

Zhang, Geng,Chen, Shouquan 한국통계학회 2012 Journal of the Korean Statistical Society Vol.41 No.4

Let {$X_n$, $n{\geq}1$} be a sequence of d-dimensional stationary Gaussian vectors, and let $M_n$ denote the maxima of {$X_k$, $1{\leq}k{\leq}n$}. Suppose that there are missing data in each component of $X_k$ and let $\tilde{M}_n$ denote the maxima of the observed variables. In this paper, we study the asymptotic distribution of the random vector ($\tilde{M}_n$, $M_n$) as the correlation and cross-correlation satisfy strongly dependent conditions.

Hydrogen storage in microwave-treated multi-walled carbon nanotubes

Geng, Hong-Zhang,Kim, Tae Hyung,Lim, Seong Chu,Jeong, Hae-Kyung,Jin, Mei Hua,Jo, Young Woo,Lee, Young Hee Elsevier 2010 International journal of hydrogen energy Vol.35 No.5

<P><B>Abstract</B></P><P>Multiwalled carbon nanotubes (MWCNTs) treated by microwave and heat treatment were used for hydrogen storage. Their storage capacity was measured using a quadruple quartz crystal microbalance in a moisture-free chamber at room temperature and at relatively low pressure (0.5MPa). Deuterium was also used to monitor the presence of moisture. The hydrogen storage capacity of the microwave-treated MWCNTs was increased to nearly 0.35wt% over 0.1wt% for the pristine sample and increased further to 0.4wt%, with improved stability after subsequent heat-treatment. The increase in the storage capacity by the microwave treatment was mostly attributed to the introduction of micropore surfaces, while the stability improvement after the subsequent heat treatment was related to the removal of functional groups. We also propose a measurement method that eliminates the moisture effect by measuring the storage capacity with hydrogen and deuterium gas.</P>

Isolation and characterization of heat-responsive gene TaGASR1 from wheat (Triticum aestivum L.)

Liyuan Zhang,Xiaoli Geng,Haiyan Zhang,Chunlei Zhou,Aiju Zhao,Fei Wang,Yue Zhao,Xuejun Tian,Zhaorong Hu,Mingming Xin,Yingyin Yao,Zhongfu Ni;Qixin Sun,Qixin Sun,Huiru Peng 한국식물학회 2017 Journal of Plant Biology Vol.60 No.1

GA-stimulated transcript (GAST) family genes have been identified in numerous plant species. In this paper, we isolated and characterized a heat-responsive gene, TaGASR1, from heat tolerant variety TAM107. The complete ORF of TaGASR1 was cloned, which encoded a 98-kDa protein, and the sequence shared 51.52% similarity to OsGASR1. Analysis of the TaGASR1 promoter region showed that it contained a heat shock element (HSE) and several cis-elements involved in abiotic stress response and hormone signal transduction. Expression patterns of TaGASR1 revealed that it was strongly induced by stress factors, such as high temperature, drought, high salinity and oxidation, as well as the phytohormones, including MeJA, ACC and ABA, which suggested the TaGASR1 gene might participate in these stress and hormone signal transduction pathways. Transient expression of TaGASR1-GFP fusion proteins in onion epidermal cells indicated that TaGASR1 protein was localized to the cell membrane or cytosol. Further analysis showed that ectopic expression of TaGASR1 in Arabidopsis enhanced thermotolerance and reduced the accumulation of reactive oxygen species (ROS) after heat stress. Moreover, we also found that TaGASR1-overexpressing wheat improved tolerance to heat stress and oxidative stress.

The sedimentary environment of Early Cretaceous rift basin in eastern China and its response to the Faraoni event

Xiangyu Zhang,Shoujun Li,Xiuli Zhao,Geng Geng,Mingming Yan 한국지질과학협의회 2020 Geosciences Journal Vol.24 No.4

To better understand the sedimentary environment of the rift basin under the influence of a warm global climate, Lingshan Island in eastern Shandong Province (China) was studied. Inorganic geochemical indexes (B, equivalent B, B/Ga, Ga, V, Couch’s palaeosalinity, Adams’s palaeosalinity, Sr/Ba, Sr/Cu, Rb/Sr, Al2O3/MgO, and CaO/MgO·Al2O3) were analyzed and a quantitative calculation of palaeosalinity was carried out based on the Adams and Couch methods. The sedimentary environment of the rift basin at Lingshan Island was determined according to the morphology and distribution of its palaeobiota as well as inorganic geochemical indexes. We demonstrate that eastern China had high-temperature drought-like conditions during the Early Cretaceous, which may have been influenced by the Faraoni event. The dry-hot climate transformed the rift basin at Lingshan Island into saline lacustrine basin. Therefore, the salinization of water was not influenced by seawater but was the response of terrestrial strata to the warm, dry climate. This study helps to understand the sedimentary background of Cretaceous rift basins in eastern China and the influence of a warm climate on China’s terrestrial strata.

Gut Microbiome Alterations and Functional Prediction in Chronic Spontaneous Urticaria Patients

( Xinyue Zhang ),( Jun Zhang ),( Zhaowei Chu ),( Linjing Shi ),( Songmei Geng ),( Kun Guo ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.5

The effects of the gut microbiome on both allergy and autoimmunity in dermatological diseases have been indicated in several recent studies. Chronic spontaneous urticaria (CSU) is a disease involving allergy and autoimmunity, and there is no report detailing the role of microbiota alterations in its development. This study was performed to identify the fecal microbial composition of CSU patients and investigate the different compositions and potential genetic functions on the fecal microbiota between CSU patients and normal controls. The gut microbiota of CSU patients and healthy individuals were obtained by 16s rRNA massive sequencing. Gut microbiota diversity and composition were compared, and bioinformatics analysis of the differences was performed. The gut microbiota composition results showed that Firmicutes, Bacteroidetes, Proteobacteria, and Verrucomicrobia were dominant microbiota in CSU patients. The differential analysis showed that relative abundance of the Proteobacteria (p = 0.03), Bacilli (p = 0.04), Enterobacterales (p = 0.03), Enterobacteriaceae (p = 0.03) was significantly increased in CSU patients. In contrast, the relative abundance of Megamonas, Megasphaera, and Dialister (all p < 0.05) in these patients significantly decreased compared with healthy controls. The different microbiological compositions impacted normal gastrointestinal functions based on function prediction, resulting in abnormal pathways, including transport and metabolism. We found CSU patients exhibited gut microbiota dysbiosis compared with healthy controls. Our results indicated CSU is associated with gut microbiota dysbiosis and pointed out that the bacterial taxa increased in CSU patients, which might be involved in the pathogenesis of CSU. These results provided clues for future microbial-based therapies on CSU.

A Hybrid Link Protection Scheme for Ensuring Network Service Availability in Link-state Routing Networks

Haijun Geng,Han Zhang,Xingang Shi,Zhiliang Wang,Xia Yin,Ju Zhang,Zhiguo Hu,Yong Wu 한국통신학회 2020 Journal of communications and networks Vol.22 No.1

The internet is playing an increasingly crucial role inboth personal and business activities. In addition, with the emergenceof real-time, delay sensitive and mission-critical applications,stringent network availability requirement is put forward for internetservice providers (ISPs). However, commonly deployed intradomainlink-state routing protocols react to link failures by globallyexchanging link state advertisements and recalculating routingtable, inevitably causing significant forwarding discontinuityafter a failure. Therefore, the loop-free criterion (LFC) approachhas been widely deployed by many ISPs for coping with the singlenetwork component failure scenario in large internet backbones. The success of LFC lies in its inherent simplicity, but this comesat the expense of letting certain failure scenarios go unprotected. To achieve full failure coverage with LFC without incurring significantextra overhead, we propose a novel link protection scheme,hybrid link protection (HLP), to achieve failure resilient routing. Compared to previous schemes, HLP ensures high network availabilityin a more efficient way. HLP is implemented in two stages. Stage one provides an efficient LFC based method (MNP-e). Thecomplexity of the algorithm is less than that of Dijkstra’s algorithmand can provide the similar network availability with LFC. Stage two provides backup path protection (BPP) based on MNP-e,where only a minimum number of links need to be protected, usingspecial paths and packet headers, to meet the network availabilityrequirement. We evaluate these algorithms in a wide spread ofrelevant topologies, both real and synthetic, and the results revealthat HLP can achieve high network availability without introducingconspicuous overhead. HLP not only needs around 10% time ofthat of full protection, but also provides full protection capabilitiesthat full protection provide.

MOLECULAR CLONING AND CHARACTERIZATION OF LEPTIN IN THE LINED SEAHORSE (Hippocampus erectus)

Huixiang Zhang,Geng Qin,Yanhong Zhang,Qiang Lin 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5

Research on Ant Colony Algorithm Optimization Neural Network Weights Blind Equalization Algorithm

Yanxiang Geng,Liyi Zhang,Yunshan Sun,Yao Zhang,Nan Yang,Jiawei Wu 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.2

The project of ant colony algorithm optimization neural network combining blind equalization algorithm is proposed. The better initial weights of neural networks are provided because of the randomness, ergodicity and positive feedback of the ant colony algorithm. And then, a combination of optimal weights are found through BP algorithm, which is fast local search speed. Thus blind equalization performance is improved. Computer simulation show that, the novel blind equalization algorithm speeds up the convergence rate, reduces the remaining steady-state error and bit error rate, which is compared with the Neural Network Blind Equalization Algorithm(NNBE) and Genetic Algorithm optimization Neural Network Blind Equalization Algorithm(GA-NNBE) .

Over expression of the wheat BEL1-like gene TaqSH1 affects floral organ abscission in Arabidopsis thaliana

Lan Zhang,Danmei Liu,Di Wang,Rongzhi Zhang,Shuaifeng Geng,Liang Wu,Aili Li,Long Mao 한국식물학회 2013 Journal of Plant Biology Vol.56 No.2

Seed shattering is one of the major domestication traits of crops. In wheat, except for the Q gene whose mutation renders free threshing and changing of rachis fragility, not much is known about the molecular mechanism for this process. We report here the cloning and characterization of TaqSH1, the ortholog of the rice seed shattering gene qSH1. TaqSH1 encodes a BEL1-like protein that is conserved between monocots and eudicots. TaqSH1 was located on the homoeologous group 3, a potential new genetic locus for seed threshability in wheat. Over expression of TaqSH1 in Arabidopsis resulted in dwarfed plants. The inflorescences of transgenic plants were more compact with larger pedicel angles. Scanning Electron Microscopy (SEM) showed that the transgenic siliques had a narrower replum where the dehiscence zone was altered. In addition, abscission of petals was significantly delayed due to delayed abscission zone development. Real-time PCR assays showed that over expression of TaqSH1 down regulated known Arabidopsis abscission related genes IDA, HAESA, KNAT1/6 and SHP1/2 in the transgenic plants. Taken together, our data suggest that TaqSH1 may represent another example of conserved mechanisms across monocots and eudicots for fruit/grain abscission and should have potential application in genetic manipulation of wheat seed shattering.