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Yoon Jeong Nam,Jeong Jun Kim,Gayoung Jeong,Ji Hee Han,Sang Yeob Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04
Sweetpotato whitefly (Bemisia tabaci), especially Q biotype, has been recognized one of the most destructive insect pests worldwide because of increased resistance to some insecticide groups requiring alternative strategies for its control. We studied the conidia production of entomopathogenic fungus Isaria javanica Pf04, which had been reported high virulence isolate against Q biotype of B. tabaci, using grain. Brown rice was most suitable for conidia mass production of the isolate of I. javanica. Conidia was produced high at 25 ~ 27.5℃. The isolate produced more spores when conidia suspension directly inoculated onto media than two-phase fermentation. When concentration of inoculum was high spore production was high, but increasing rate of conidia production was highest at low inoculum concentration (1×105 conidia/ml) as 6,700 times increase compared with 20 times increase at high inoculum concentration (1×108 conidia/ml). These results indicated that the isolate can produce more conidia with cheap agricultural product and can develop as a microbial pesticide to control sweetpotato whitefly.
Quinolone-resistant Shigella flexneri Isolated in a Patient Who Travelled to India
You La Jeon,You-sun Nam,Gayoung Lim,조선영,Yun-Tae Kim,Ji-Hyun Jang,Junyoung Kim,Misun Park,이희주 대한진단검사의학회 2012 Annals of Laboratory Medicine Vol.32 No.5
We report a recent case in which ciprofloxacin-resistant Shigella flexneri was isolated from a 23-yr-old female patient with a history of travel to India. Prior to her admission to our internal medicine department, she experienced symptoms of high fever and generalized weakness from continuous watery diarrhea that developed midway during the trip. S. flexneri was isolated from the stool culture. Despite initial treatment with ciprofloxacin, the stool cultures continued to show S. flexneri growth. In the susceptibility test for antibiotics of the quinolone family, the isolate showed resistance to ciprofloxacin(minimum inhibitory concentration [MIC], 8 μg/mL), norfloxacin (MIC, 32 μg/mL), ofloxacin (MIC, 8 μg/mL),nalidixic acid (MIC, 256 μg/mL), and intermediate resistance to levofloxacin (MIC, 4 μg/mL). In molecular studies for quinolone resistance related genes, plasmid borne-quinolone resistance genes such as qnrA, qnrB, qnrS, aac(6’)-Ib-cr, qepA, and oqxAB were not detected. Two mutations were observed in gyrA (248C→T, 259G→A) and 1 mutation in parC (239G→T). The molecular characteristics of the isolated S. flexneri showed that the isolate was more similar to the strains isolated from the dysentery outbreak in India than those isolated from Korea.