DSP를 이용한 Trapezoidal형 브러시리스 직류 전동기의 가변 구조 위치제어기 설계

千熙英(Hee-Young Chun),朴貴泰(Gwi-Gae Park),宋明現(Myung-Hyun Song),呂炯基(Hyeong-Gee Yeo),高輔亨(Po-Hyoung Koh),李相樂(Sang-Lak Lee) 대한전기학회 1991 전기학회논문지 Vol.40 No.10

Sensitive on-chip detection of cancer antigen 125 using a DNA aptamer/carbon nanotube network platform

Gedi, Vinayakumar,Song, Chung Kil,Kim, Gae Baik,Lee, Jin Oh,Oh, Eunkyul,Shin, Bum Seok,Jung, Mingi,Shim, Jinhee,Lee, Haiwon,Kim, Young-Pil Elsevier 2018 Sensors and actuators. B Chemical Vol.256 No.-

<P><B>Abstract</B></P> <P>Despite the major role of cancer antigen 125 (CA125) in cancer progression, its structural diversity makes antibody-based immunoassay of this protein difficult. Here we report the selection of an anti-CA125 ssDNA aptamer and its application to sensitive detection of CA125 when used together with a CA125-specific antibody immobilized on a three-dimensional network of carbon nanotubes (3DN-CNTs). With exceptional stability and easy modifications over antibodies, the selected ssDNA aptamer (rCAA-8) showed high binding affinity (166nM) for recombinant CA125, which enabled CA125-specific imaging in ovarian cancer cells (OVCAR-3). Furthermore, when compared to other fluorescent assays based on graphene oxide or flat surface and traditional enzyme-linked immunosorbent assay, a chip-based assay using a 3DN-CNT surface and anti-CA125 antibody-aptamer pair resulted in higher sensitivity and broader dynamic range as a function of CA125 concentration, due to high target specificity and high surface loading density. Our on-chip aptamer-based assay will facilitate sensitive and specific monitoring of CA125 in the biological and clinical fields.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The ssDNA aptamer for cancer antigen 125 (CA125) was selected through SELEX. </LI> <LI> Specificity of aptamer to CA125 was validated using cell imaging and 3DN-CNT. </LI> <LI> Biochip platform showed much improved sensitivity than that of 2D or ELISA. </LI> </UL> </P>

Tolerogenic dendritic cells show gene expression profiles that are different from those of immunogenic dendritic cells in DBA/1 mice

Lee, Eun Gae,Jung, Nam-Chul,Lee, Jun-Ho,Song, Jie-Young,Ryu, Sang-Young,Seo, Han Geuk,Han, Sung Gu,Ahn, Keun Jae,Hong, Kwan Soo,Choi, Jinjung,Lim, Dae-Seog Informa UK (Informa Healthcare) 2016 Autoimmunity Vol.49 No.2

Fluorescing aptamer-gold nanosensors for enhanced sensitivity to bisphenol A

Lee, Eun-Song,Kim, Gae Baik,Ryu, Su-Hyun,Kim, Hyeon,Yoo, Hye Hyun,Yoon, Moon Young,Lee, Jin-Won,Gye, Myung Chan,Kim, Young-Pil Elsevier 2018 Sensors and actuators. B Chemical Vol.260 No.-

<P><B>Abstract</B></P> <P>An insufficient sensitivity of aptamer-integrated colorimetric gold nanoparticles (AuNPs) is a common challenge in the detection of environmental chemicals. We report the use of a modified aptamer/AuNP-based sensor in conjunction with a fluorescing single-stranded DNA aptamer for the sensitive detection of bisphenol A (BPA), which is a major endocrine disruptor (EDC). While an anti-BPA single-stranded DNA aptamer was bound with BPA, a weak fluorescence signal was observed upon the addition of SYBR Green-I (SG-I), which is specific to a duplex region of the free aptamer. This reaction was accompanied by a subsequent aggregation of unmodified AuNPs (citrate-stabilized AuNPs) at high salt concentrations, leading to a color change from reddish to purple. In contrast, the absence of BPA elicited a high fluorescence signal from SG-I and produced no color change in the AuNP solution even upon the addition of salt ions. Unlike aptamers that are labeled at their ends with fluorophores, our strategy gave rise to neither a loss of target-binding affinity nor a perturbation of the AuNP colorimetry. Thus, a quantitative analysis with a broad dynamic range was achieved by combining the fluorescent and colorimetric measurements at BPA concentrations ranging over four orders of magnitude. Our approach also yielded a greater detection sensitivity (as low as 9 pg·mL<SUP>−1</SUP>) than classical AuNP colorimetry or other aptamer-combined methods Moreover, this method enabled the detection of BPA in a small fraction extracted from thermal paper with a high specificity among EDCs. We anticipate that this approach will facilitate further advances in the design of traditional AuNP colorimetric sensors using different aptamers for chemical sensing in the environmental and clinical fields.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Aptamer-facilitated AuNP sensors was implemented for the detection of BPA. </LI> <LI> Fluorescing ssDNA aptamer/AuNPs enabled the improved detection of BPA. </LI> <LI> This method exceeds the sensitivity of classical colorimetry or fluorescent method. </LI> </UL> </P>

종이/페놀수지가 주성분인 동박적층판(Copper Clad Laminate)의 열분해 특성

송재헌(Jae Hun Song),김승도(Seung Do Kim),안현철(Hyun Cheol Ahn),김경수(Gyung Soo Kim),김상범(Sang Bum Kim),정재성(Jae Sung Jung),공성호(Sung Ho Gong),조영개(Young Gae Cho) 大韓環境工學會 2007 대한환경공학회지 Vol.29 No.9

본 논문의 목적은 최근 발생량이 급속히 증가하고 있으나, 적절한 처리방법이 없는 인쇄회로기판의 원자재인 페놀수지 동박적층판(Phenol Copper Clad Laminate, 이하 p-CCL)의 재활용을 위해 열분해 적용 가능성을 조사하는데 있다. 동역학 특성은 열중량분석기(Thermogravimetric Analyzer, 이하 TGA)를 사용하였다. 또한 280, 350, 600℃의 온도에서 생성된 액체와 고체부산물에 대해서는 원소분석, 공업분석, 발열량 등의 일반적인 특성을 분석하였고, 액체부산물은 GC/MS, FT-IR를 이용하여 구성 성분을 분석하였다. TGA 결과에 의하면 p-CCL의 분해는 세 단계에 걸쳐 일어났다. 첫 번째는 280℃ 이하의 저온 분해구간, 280∼350℃의 중온분해구간, 350℃ 이상의 고온 분해구간으로 구분할 수 있다. 저온, 중온에서의 액체부산물의 주요 성분은 수분과 페놀인 반면에 고온에서는 가지가 있는 페놀류와 퓨란류로 나타났다. 반응온도가 높아짐에 따라 휘발성분의 양은 감소하는 반면 고정탄소의 함량은 증가하였다. 고체부산물의 고위 발열량은 7,400∼7,600 kcal/kg으로 연료로서의 활용 가능성이 있다고 여겨진다. 또한 고체부산물의 고정탄소 함량이 높고, 회분 성분 함량이 낮기 때문에 적절한 개질화 과정을 거친다면 흡착제로서도 활용 가능하다고 판단된다. Electronic wastes have increased tremendously. However, any reliable treatment methodologies have rarely been established. Electronic wastes have posed serious disposal problem due to their physico-chemical stability. This paper investigated the application possibility of pyrolysis for the purpose of recycling the p-CCL(phenol based Copper Clad Laminate). Thermogravimetric analysis(TGA) was used to investigate the thermal decomposition pattern of p-CCL. We elucidated the characteristics of pyrolysis by-products at operating temperatures of 280, 350 and 600℃. GC/MS and FT-IR were used to characterize the liquid by-products along with general characterization methods such as Ultimate Analysis, Proximate Analysis and Heating Value, whereas general characterization methods were only introduced for the solid by-products. At a heating rate of 5℃/min, TGA curves exhibited three decomposition stages: (1) low-temperature decomposition region(<280℃), (2) medium temperature region(280∼350℃) and (3) high-temperature region(>350℃). The major compounds of liquid by-products at low- and medium-temperatures were accounted for by water and phenol, whereas branched phenols and furans were major compounds at high-temperatures. As the temperature increases, volatile quantities decreased but the fixed carbon increased. High heating values of solid by-products(7,400∼7,600 kcal/kg) would suggest that the solid by-products could be applicable as fuel. In addition, high fixed carbon but low ash content of the solid by-products offered an implication that they are capable of being upgradable for adsorbent after applying appropriate activating process.

위눈꺼풀에 발생한 아포크린샘암종 1예

임형빈,김경남,송계용,이성복,Hyung Bin Lim,Kyoung Nam Kim,Gae Young Song,Sung Bok Lee 대한안과학회 2014 대한안과학회지 Vol.55 No.3

Purpose: To report a case involving an eyelid mass that was diagnosed as apocrine carcinoma. Case summary: A 52-year-old man visited our hospital with a recurrent mass on his right upper eyelid, which had developed 4 years prior. Initially, he received laser therapy at a dermatologic clinic to remove the mass. Two years later, the mass recurred and was excised at another clinic. At the time the patient visited our institution, the lesion had developed into multiple erythematous nodules at the margin of the right upper eyelid. The results of excisional biopsy performed under local anesthesia revealed hidradenoma papilliferum. One month after excision, recurred multiple elevated nodules were found at the margin of the excision, and thus total excision of the mass and reconstruction of the upper eyelid was performed. Biopsy confirmed that the mass was apocrine adenocarcinoma. Five months have passed since the excision and no evidence of recurrence has been observed. Conclusions: Apocrine adenocarcinoma is a malignant tumor of the sweat gland and is rarely found on the eyelid. Apocrine adenocarcinoma should be considered in the differential diagnosis of recurrent eyelid mass at the eyelid margin. J Korean Ophthalmol Soc 2014;55(3):432-436

Myosin-primed tolerogenic dendritic cells ameliorate experimental autoimmune myocarditis

Lee, Jun-Ho,Kim, Tae-Hoon,Park, Hyo Eun,Lee, Eun Gae,Jung, Nam-Chul,Song, Jie-Young,Seo, Han Geuk,Seung, Ki-Bae,Chang, Kiyuk,Lim, Dae-Seog Oxford University Press 2014 Cardiovascular research Vol.101 No.2

<P><B>Aims</B></P><P>Autoimmunity plays an important role in the pathogenesis of viral myocarditis and giant cell myocarditis. Experimental autoimmune myocarditis (EAM) is a mouse model of myocarditis that is induced by cardiac myosin. Tolerogenic dendritic cells (tDCs) are used as anti-inflammatory and immunosuppressive targets in a number of autoimmune disease models, but their effect on EAM has not been addressed. The aim of this study was to investigate whether tDC therapy in an EAM mouse model can suppress inflammatory myocarditis, a potential precursor of dilated cardiomyopathy.</P><P><B>Methods and results</B></P><P>tDCs were generated by treating immature DCs (imDCs) with TNF-α and cardiac myosin. Mice with EAM were injected twice with tDCs (with a 1-week interval) at three doses (2 × 10<SUP>5</SUP>, 1 × 10<SUP>6</SUP>, or 2 × 10<SUP>6</SUP>). The severity of myocarditis was histopathologically assessed. The phenotypes of the DC and regulatory T (Treg) cell populations were determined by flow cytometry and the effect of tDCs on autoimmunity-inducing cytokines was examined by ELISA. Myosin-pulsed tDCs displayed lower levels of DC-related surface markers and expressed higher levels of indoleamine 2, 3-dioxygenase (IDO) than mature DCs (mDCs). Histopathological examination revealed that hearts from tDC-treated mice showed markedly reduced myocardial inflammation compared with those of untreated EAM mice. These therapeutic effects by tDCs were mediated at least by enhanced myosin-specific Treg cell induction and anti-inflammatory cytokine secretion.</P><P><B>Conclusion</B></P><P>Taken together, these results show for the first time that myosin-pulsed tDCs ameliorate EAM, and that this occurs most likely via the induction of antigen-specific Treg cells.</P>

습식분류층 석탄가스화기 수치해석 및 실험적 연구

김혜숙(Hey Suk Kim),최승희(Seung Hee Choi),황민정(Min Jung Hwang),송우영(Woo Young Song),신미수(Mi Soo Shin),장동순(Dong Soon Jang),윤상준(Sang June Yun),최영찬(Young Chan Choi),이재구(Gae Goo Lee) 大韓環境工學會 2010 대한환경공학회지 Vol.32 No.2

간세포암의 전이 표지자로서 말초 혈액내 알부민 mRNA 검출의 의의

유병철,김정아,서동진,박능화,정영화,이영상,고문수,송일한,이근찬,추윤호,안계순 대한소화기학회 1999 대한소화기학회지 Vol.33 No.2

Background/Aims: Albumin mRNA, one of hepatocyte-specific genes, in peripheral blood has been reported as a marker of circulating hepatocytes in hepatocellular carcinoma (HCC). We evaluated the correlation between albumin mRNA in peripheral blood and evident vascular invasion or extrahepatic metastasis of HCC, and characterized the tumor and clinical features of patients with HCC in association with albumin mRNA in circulation. Methods: We analyzed albumin m-RNA in peripheral blood of 62 patients with hepatocellular carcinoma, 18 patients with chronic viral hepatitis and 8 patients with metastatic liver cancer. Also, we compared the radiological, clinical and tumor characteristics o patients with hepatocellular carcinoma. Results: In 36 of 62 patients with HCC (58%), 5 of 18 patients with chronic viral hepatitis (28%) and none of patients with metastatic liver cancer (0%) albumin mRNA was detected in peripheral blood. We analysed 61 patients with HCC (35 positive and 26 negative for albumin mRNA in peripheral circulation). We did not examine the remaining one patient whose serum ALT level was above 200 IU/L to reduce the possibility of false positive resul by severe hepatic necrosis. Conclusions: The HCC patients with albumin mRNA in peripheral blood were younger than those without it (55 ±9 vs 50 ±9; p=0.03). The rates of serum HBsAg positivity were higher in the patients with albumin mRNA in pheripheral blood than in those without it. Mos of HCC patients with extrahepatic metastasis (17/18; 84%), evident portal vein involvement (26/31 84%), and biliary invasion (9/9; 100%) had albumin mRNA in peripheral circulation. Albumin mRNA was detected more commonly in patients with tumor over 5 cm in diameter than in patients with tumor less 5 cm (76% vs 20%; p$lt;0.001). Moreover, albumin mRNA was more commonly detected in infiltrative type of tumor than in nodular type (90% vs 43%; p$lt;0.001). (Kor J Gastroentero 1999;33:240 - 251)