A Possible Significance in Vertebrate Phototransduction of Multi-Protein Signaling Complexes on Raft-Like Membranes

Hayashi, Fumio,Liu, Han,Seno, Keiji Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2

Raft is a distinctive membrane domain enriched in a certain class of lipids, cholesterol, and proteins observed on the plasma membrane. Growing evidence has revealed that such membrane domains play key roles in signal transduction, fertilization, development, transmitter release, and so on. Recently, we have isolated raft-like detergent-resistant membrane (DRM) fraction from bovine photoreceptor rod outer segments. Transducin and its effecter, cGMP-phosphodiesterase, elicited stimulus-dependent translocation between detergent-soluble membrane and DRM. This suggested potential importance of such distinct membrane domains in vertebrate phototransduction. Here, we will discuss physiological meaning of the translocation of major components of cGMP cascade to raft-like membrane in phototransduction. We would like to propose a hypothesis that raft-like membrane domains on the disk membrane are the place where cGMP cascade system could be quenched.

Loss Analysis of Hybrid Electric Vehicle Inverters

Fumio Asakura,Norihito Kimura,Yuji Hayashi 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

The analysis of electrical losses of hybrid electric vehicle (HEV) inverters is significant for improved fuel efficiency for HEVs. To analyze the breakdown of electrical losses in LA-4 Cycle or under other similar driving conditions, measurement and calculation methods have been developed to ascertain losses attributable to different parts of HEV inverters. To determine electrical losses, the methods use voltages and currents in HEV inverters measured with a vehicle-mounted virtual and real simulator (VRS)

The antlions of the Dendroleon pupillaris group (Neuroptera, Myrmeleontidae, Dendroleontinae), with description of three new species from China

Zheng Yuchen,Hayashi Fumio,Matsumoto Rikio,Liu Xingyue 한국응용곤충학회 2024 Journal of Asia-Pacific Entomology Vol.27 No.1

An antlion species-group of Dendroleon Brauer, 1866, namely the D. pupillaris group, is defined, including five species (two described species and three new species): D. jianyuni Zheng & Liu sp. nov., D. motuoensis Wang & Wang, 2008, D. pupillaris (Gerstaecker, 1893), D. wenii Zheng, Hayashi, Matsumoto & Liu sp. nov., and D. xiaohani Zheng & Liu sp. nov. We sequenced the DNA barcodes of all species to test our morphological species delimitation. The immature stage of D. jianyuni sp. nov. is also described. In addition, the generic diagnosis of Dendroleon is further discussed. https://www.zoobank.org/zoobank.org:pub:91282009-E5D4-41EB-BCD5-2B96AA4D6516.

Signaling Protein Complex Formation in Detergent Resistant Membrane of Bovine Photoreceptor Rod Outer Segments

Liu, Han,Seno, Keiji,Hayashi, Fumio Korean Society of Photoscience 2002 Journal of Photosciences Vol.9 No.2

We have recently found that a detergent-resistant raft like membrane (DRM) can be prepared from bovine rod outer segment membranes as a low-density buoyant fraction in sucrose density gradient ultracentrifugation. G protein (transducin) and its effector enzyme (phosphodiesterase: PDE) drastically change their affinities to DRM in the process of phototransduction. We report here that the recruitment of transducin and/or $^2$PDE to DRM has close relationship with their states in signal transduction. Active T$\alpha$/PDE-complex has a high affinity to DRM, whereas inactive transducin, or inactive PDE are excluded from DRM. Active T$\alpha$/PDE-complex seems to bind to a GTPase activating protein (GRS9) in multi- protein complexes localized on DRM. Physiological significance of the multi-protein complex on the raft-like membrane in vertebrate phototransduction would be discussed.

Vertebral Lateral Notch as Optimal Entry Point for Lateral Mass Screwing Using Modified Roy-Camille Technique

Norio Yamamoto,Hirofumi Kosaka,Kosaku Higashino,Masatoshi Morimoto,Kazuta Yamashita,Fumitake Tezuka,Fumio Hayashi,Yoichiro Takata,Toshinori Sakai,Akihiro Nagamachi,Koichi Sairyo 대한척추외과학회 2018 Asian Spine Journal Vol.12 No.2

Study Design: Retrospective study of 37 consecutive female patients with cervical spondylotic myelopathy who underwent reconstructed computed tomography (CT) scanning of the cervical spine. Purpose: The purpose of this study was to investigate whether the vertebral lateral notch of the cervical spine is an effective landmark to determine the entry point for lateral mass screwing. A modified Roy-Camille technique was used to determine the entry point associated with the lateral notch of the cervical spine. Overview of Literature: The Roy-Camille technique has been a popular technique for the posterior fixation of the cervical spine. A problem with this technique is determining the entry point on the lateral mass via visual inspection, such as in cases with degenerative or destructive cervical facet joints. Methods: Thirty-three female patients with cervical spondylotic myelopathy underwent reconstructed CT scanning of the cervical spine. Overall, 132 vertebrae from C3 to C6 were reviewed using reconstructed CT. The probable trajectory using a modified Roy- Camille technique was determined using reconstructed CT scans, and the optimal entry point was identified. Horizontal and vertical distances from the vertebral lateral notch were measured. Results: The entry point determined using the modified Roy-Camille technique was significantly superior and medial compared with that determined using the conventional Roy-Camille technique. At C3 and C4 levels, the entry point using the modified technique was 1.4 mm below and 4.4 mm medial to the lateral notch, and at C5 and C6 levels, it was 2.3 mm below and 4.9 mm medial to the lateral notch. Conclusions: The vertebral lateral notch of the cervical spine was an effective landmark to determine the entry point for lateral mass screwing. The modified Roy-Camille technique proposed here may prevent surgical complications and poor outcomes.

Influence of Signal Intensity Non-Uniformity on Brain Volumetry Using an Atlas-Based Method

Masami Goto,Osamu Abe,Tosiaki Miyati,Hiroyuki Kabasawa,Hidemasa Takao,Naoto Hayashi,Tomomi Kurosu,Takeshi Iwatsubo,Fumio Yamashita,Hiroshi Matsuda,Harushi Mori,Akira Kunimatsu,Shigeki Aoki,Kenji Ino,K 대한영상의학회 2012 Korean Journal of Radiology Vol.13 No.4

Objective: Many studies have reported pre-processing effects for brain volumetry; however, no study has investigated whether non-parametric non-uniform intensity normalization (N3) correction processing results in reduced system dependency when using an atlas-based method. To address this shortcoming, the present study assessed whether N3 correction processing provides reduced system dependency in atlas-based volumetry. Materials and Methods: Contiguous sagittal T1-weighted images of the brain were obtained from 21 healthy participants, by using five magnetic resonance protocols. After image preprocessing using the Statistical Parametric Mapping 5 software, we measured the structural volume of the segmented images with the WFU-PickAtlas software. We applied six different bias-correction levels (Regularization 10, Regularization 0.0001, Regularization 0, Regularization 10 with N3, Regularization 0.0001 with N3, and Regularization 0 with N3) to each set of images. The structural volume change ratio (%) was defined as the change ratio (%) = (100 x [measured volume - mean volume of five magnetic resonance protocols] / mean volume of five magnetic resonance protocols) for each bias-correction level. Results: A low change ratio was synonymous with lower system dependency. The results showed that the images with the N3 correction had a lower change ratio compared with those without the N3 correction. Conclusion: The present study is the first atlas-based volumetry study to show that the precision of atlas-based volumetry improves when using N3-corrected images. Therefore, correction for signal intensity non-uniformity is strongly advised for multi-scanner or multi-site imaging trials. Objective: Many studies have reported pre-processing effects for brain volumetry; however, no study has investigated whether non-parametric non-uniform intensity normalization (N3) correction processing results in reduced system dependency when using an atlas-based method. To address this shortcoming, the present study assessed whether N3 correction processing provides reduced system dependency in atlas-based volumetry. Materials and Methods: Contiguous sagittal T1-weighted images of the brain were obtained from 21 healthy participants, by using five magnetic resonance protocols. After image preprocessing using the Statistical Parametric Mapping 5 software, we measured the structural volume of the segmented images with the WFU-PickAtlas software. We applied six different bias-correction levels (Regularization 10, Regularization 0.0001, Regularization 0, Regularization 10 with N3, Regularization 0.0001 with N3, and Regularization 0 with N3) to each set of images. The structural volume change ratio (%) was defined as the change ratio (%) = (100 x [measured volume - mean volume of five magnetic resonance protocols] / mean volume of five magnetic resonance protocols) for each bias-correction level. Results: A low change ratio was synonymous with lower system dependency. The results showed that the images with the N3 correction had a lower change ratio compared with those without the N3 correction. Conclusion: The present study is the first atlas-based volumetry study to show that the precision of atlas-based volumetry improves when using N3-corrected images. Therefore, correction for signal intensity non-uniformity is strongly advised for multi-scanner or multi-site imaging trials.

TP53 variants in p53 signatures and the clonality of STICs in RRSO samples

Tomoko Akahane,Kenta Masuda,Akira Hirasawa,Yusuke Kobayashi,Arisa Ueki,Miho Kawaida,Kumiko Misu,Kohei Nakamura,Shimpei Nagai,Tatsuyuki Chiyoda,Wataru Yamagami,Shigenori Hayashi,Fumio Kataoka,Kouji Ban 대한부인종양학회 2022 Journal of Gynecologic Oncology Vol.33 No.4

Objective: Precursor lesions may be identified in fallopian tube tissue after risk-reducing salpingo-oophorectomy (RRSO) in patients with pathogenic variants of . Serous tubal intraepithelial carcinoma (STIC) is considered a precursor of high-grade serous carcinoma, whereas the significance of the p53 signature remains unclear. In this study, we investigated the relationship between the p53 signature and the risk of ovarian cancer. Methods: We analyzed the clinicopathological findings and conducted DNA sequencing for variants of p53 signatures and STIC lesions isolated using laser capture microdissectionin 13 patients with pathogenic variants of who underwent RRSO and 17 control patients with the benign gynecologic disease. Results: pathogenic variants were detected significantly higher in RRSO group than control (p<0.001). No difference in the frequency of p53 signatures were observed between groups (53.8% vs 29.4%; p=0.17). sequencing and next-generation sequencing analysis in a patient with STIC and occult cancer revealed 2 mutations causing different p53 staining for STICs and another mutation shared between STIC and occult cancer. Conclusion: The sequence analysis for revealed 2 types of p53 signatures, one with a risk of progression to STIC and ovarian cancer with pathological variants in and the other with a low risk of progression without pathological variants in as seen in control.