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Molecular Cloning and Bioinformatic Analysis of SPATA4 Gene
Liu, Shang-Feng,Ai, Chao,Ge, Zhong-Qi,Liu, Hai-Luo,Liu, Bo-Wen,He, Shan,Wang, Zhao Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.6
Full-length cDNA sequences of four novel SPATA4 genes in chimpanzee, cow, chicken and ascidian were identified by bioinformatic analysis using mouse or human SPATA4 cDNA fragment as electronic probe. All these genes have 6 exons and have similar protein molecular weight and do not localize in sex chromosome. The mouse SPATA4 sequence is identified as significantly changed in cryptorchidism, which shares no significant homology with any known protein in swissprot databases except for the homologous genes in various vertebrates. Our searching results showed that all SPATA4 proteins have a putative conserved domain DUF1042. The percentages of putative SPATA4 protein sequence identity ranging from 30% to 99%. The high similarity was also found in 1 kb promoter regions of human, mouse and rat SPATA4 gene. The similarities of the sequences upstream of SPATA4 promoter also have a high proportion. The results of searching SymAtlas (http://symatlas.gnf.org/SymAtlas/) showed that human SPATA4 has a high expression in testis, especially in testis interstitial, leydig cell, seminiferous tubule and germ cell. Mouse SPATA4 was observed exclusively in adult mouse testis and almost no signal was detected in other tissues. The pI values of the protein are negative, ranging from 9.44 to 10.15. The subcellular location of the protein is usually in the nucleus. And the signal peptide possibilities for SPATA4 are always zero. Using the SNPs data in NCBI, we found 33 SNPs in human SPATA4 gene genomic DNA region, with the distribution of 29 SNPs in the introns. CpG island searching gives the data about CpG island, which shows that the regions of the CpG island have a high similarity with each other, though the length of the CpG island is different from each other.This research is a fundamental work in the fields of the bioinformational analysis, and also put forward a new way for the bioinformatic analysis of other genes.
Recycling of automotive shredder residue by solid state shear milling technology
Shuangqiao Yang,Feng Zhong,Meng Wang,Shibing Bai,Qi Wang 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.57 No.-
The main compositions in automotive shredder residue (ASR) were polymers, up to about 70 wt%, whichis difficult to be separated and classified. In this work, ultrafine ASR powders was prepared through amechanochemical technology called solid state shear milling (S3M) and the melt blending process wasfurther applied to produce value-added polypropylene (PP) composites. Both optical and electronmicroscopy revealed the excellent dispersion and in-situ compatibilization of ASR were achieved in thePP/ASR composites. After S3M treatment the tensile strength, elongation at break and charpy notchedimpact strength of PP/ASR composites were increased by 39%, 285% and 50%, respectively. Moreover, themechanical properties of the composites were even superior to those with addition of PP-g-MAH (acommercial compatibilizer). The ASR recycling strategy proposed here is simple, low cost, efficient andgreen, exhibiting great potential for fabricating non-structural products used in the realms of containerfor waste, plastic garbage bags and the road stone.
Mei-rong Zhou,Zhong-hua Tang,Jing Li,Jin-Hu Fan,Yi Pang,Hong-jian Yang,Shan Zheng,Jing-qiao Bai,Ning Lv,You-Lin Qiao,Feng Xu,Hai-zhi Qi 한국유방암학회 2013 Journal of breast cancer Vol.16 No.1
Purpose: This study aims to analyze the clinical-pathological characteristics of multifocal and multicentric breast cancer (MMBC) in Chinese women. Methods: Sixty-seven cases with MMBC were randomly collected and reviewed at seven hospitals in representative districts of China during 1999 to 2008. Results: The incidence of MMBC in breast cancer in China was 1.75%. Compared to those with unifocal breast cancer, women with MMBC were more likely to have larger tumor size, lymph node metastasis (59.70% vs. 45.62%) and stage III to IV (46.26% vs. 21.10%). The peak age at onset of MMBC was 40 to 49 years old and has been gradually increasing during 1999 to 2008. Most of the MMBC women were treated with surgery and adjuvant therapy. Conclusion: In China, the incidence of MMBC in breast cancer is significantly lower than that in Western countries. Compared to unifocal breast cancer, MMBC is biologically more aggressive. Most MMBC women underwent mastectomy, instead of breast conservation surgery.
Flexural stiffness of steel-concrete composite beam under positive moment
Fa-Xing Ding,Jing Liu,Xuemei Liu,Feng-Qi Guo,Li-Zhong Jiang 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.20 No.6
This paper investigates the flexural stiffness of simply supported steel-concrete composite I-beams under positive bending moment through combined experimental, numerical, and different standard methods. 14 composite beams are tested for experimental study and parameters including shear connection degree, transverse and longitudinal reinforcement ratios, loading way are also investigated. ABAQUS is employed to establish finite element (FE) models to simulate the flexural behavior of composite beams. The influences of a few key parameters, such as the shear connection degree, stud arrangement, stud diameter, beam length, loading way, on the flexural stiffness is also studied by parametric study. In addition, three widely used standard methods including GB, AISC, and British standards are used to estimate the flexural stiffness of the composite beams. The results are compared with the experimental and numerical results. The findings have provided comprehensive understanding of the flexural stiffness and the modelling of the composite beams. The results also indicate that GB 50017-2003 could provide better results in comparison to the other standards.