Osthol and imperatorin from Cnidium monnieri fruits enhance adipocyte differentiation in 3T3-L1 cells

Eunjin Shin, Bang Yeon Hwang, Mi Kyeong Lee 충북대학교 약품자원개발연구소 2010 약학논문집 Vol.24 No.-

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Antifibrotic activity of coumarins from Cnidium monnieri fruits in HSC-T6 hepatic stellate cells

Shin, Eunjin,Lee, Chul,Sung, Sang Hyun,Kim, Young Choong,Hwang, Bang Yeon,Lee, Mi Kyeong Springer-Verlag 2011 Journal of Natural Medicines Vol.65 No.2

Aerial Parts and Roots of Pulsatilla koreana Affect the Viability of HSC-T6 Hepatic Stellate Cells

Eunjin Shin,Qing Liu,성상현,Young Choong Kim,황방연,이미경 한국생약학회 2010 Natural Product Sciences Vol.16 No.4

During liver fibrosis, hepatic stellate cells (HSCs) undergo a complex activation process characterized by increased proliferation and extracellular matrix deposition, which is the major pathological feature of hepatic cirrhosis. Therefore, suppression of HSCs activation has been proposed as therapeutic strategies for hepatic fibrosis. We tried to screen the antifibrotic activity of natural products employing HSC-T6, hepatic stellate cell lines as an in vitro assay system. In the present study, we investigated the antiproliferative activity of aerial parts and roots of Pulsatilla koreana Nakai (Ranunculaceae). Our present study shows that roots of P. koreana exerted more strong inhibitory activity compared to its aerial parts. In addition, among the fractions of the aqueous methanolic extract of P. koreana roots, both n-hexane and CHCl3 fraction showed the strong inhibitory activity on HSC proliferation. Further study also demonstrated that the n-hexane and CHCl3 fraction of P. koreana roots significantly inhibited the HSC proliferation in time- and concentration-related manners.

Inhibitory Effects of Coumarins from the Stem Barks of <i>Fraxinus rhynchophylla</i> on Adipocyte Differentiation in 3T3-L1 Cells

Shin, Eunjin,Choi, Kyeong-Mi,Yoo, Hwan-Soo,Lee, Chong-Kil,Hwang, Bang Yeon,Lee, Mi Kyeong Pharmaceutical Society of Japan 2010 Biological & pharmaceutical bulletin Vol.33 No.9

<P>In the course of screening anti-adipogenic activity of natural products employing the preadipocyte cell line, 3T3-L1 as an <I>in vitro</I> assay system, the EtOAc fraction of the stem barks of <I>Fraxinus rhynchophylla</I> D<SMALL>ENCE</SMALL> (Oleaceae) showed significant inhibitory activity on adipocyte differentiation as assessed by measuring fat accumulation using Oil Red O staining. Activity-guided fractionation led to the isolation of six coumarins such as esculetin (1), scopoletin (2), fraxetin (3), fraxidin (4) esculin (5) and fraxin (6). Among the six coumarins isolated, esculetin (1) showed the most potent inhibitory activity on adipocyte differentiation, followed by fraxetin (3). Further studies with interval treatment demonstrated that esculetin (1) exerted inhibitory activity on adipocyte differentiation when treated within 2 d (days 0—2) after differentiation induction. We further investigated the effect of esculetin (1) on peroxisome proliferator activated receptor γ (PPARγ), one of the early adipogenic transcription factors. Esculetin (1) significantly blocked the induction of PPARγ protein expression and inhibited adipocyte differentiation induced by troglitazone, a PPARγ agonist. Taken together, these results suggest that esculetin (1), an active compound from <I>F. rhynchophylla</I>, inhibited early stage of adipogenic differentiation, in part, <I>via</I> inhibition of PPARγ-dependent pathway.</P>

Lower serum uric acid level predicts mortality in dialysis patients

Bae, Eunjin,Cho, Hyun-Jeong,Shin, Nara,Kim, Sun Moon,Yang, Seung Hee,Kim, Dong Ki,Kim, Yong-Lim,Kang, Shin-Wook,Yang, Chul Woo,Kim, Nam Ho,Kim, Yon Su,Lee, Hajeong Wolters Kluwer Health 2016 Medicine Vol.95 No.24

<▼1><P>Supplemental Digital Content is available in the text</P></▼1><▼2><P><B>Abstract</B></P><P>We evaluated the impact of serum uric acid (SUA) on mortality in patients with chronic dialysis. A total of 4132 adult patients on dialysis were enrolled prospectively between August 2008 and September 2014. Among them, we included 1738 patients who maintained dialysis for at least 3 months and had available SUA in the database. We categorized the time averaged-SUA (TA-SUA) into 5 groups: <5.5, 5.5–6.4, 6.5–7.4, 7.5–8.4, and ≥8.5 mg/dL. Cox regression analysis was used to calculate the hazard ratio (HR) of all-cause mortality according to SUA group. The mean TA-SUA level was slightly higher in men than in women. Patients with lower TA-SUA level tended to have lower body mass index (BMI), phosphorus, serum albumin level, higher proportion of diabetes mellitus (DM), and higher proportion of malnourishment on the subjective global assessment (SGA). During a median follow-up of 43.9 months, 206 patients died. Patients with the highest SUA had a similar risk to the middle 3 TA-SUA groups, but the lowest TA-SUA group had a significantly elevated HR for mortality. The lowest TA-SUA group was significantly associated with increased all-cause mortality (adjusted HR, 1.720; 95% confidence interval, 1.007–2.937; <I>P</I> = 0.047) even after adjusting for demographic, comorbid, nutritional covariables, and medication use that could affect SUA levels. This association was prominent in patients with well nourishment on the SGA, a preserved serum albumin level, a higher BMI, and concomitant DM although these parameters had no significant interaction in the TA-SUA-mortality relationship except DM. In conclusion, a lower TA-SUA level <5.5 mg/dL predicted all-cause mortality in patients with chronic dialysis.</P></▼2>

잠재전이분석과 머신러닝 기법을 적용한 초-중 학교급 전환에 따른 자기조절학습 변화양상과 예측변인 탐색

신은진(Eunjin Shin),손원숙(Wonsook Sohn) 한국교육평가학회 2023 교육평가연구 Vol.36 No.4

일본 여자축구팀의 성통제방법에 관한 사회학적 연구

신은진(Shin, Eunjin) 한국사회학회 2018 한국사회학회 사회학대회 논문집 Vol.2018 No.12

본 연구의 목적은, 일본 여자축구 K팀에서의 현장연구를 통해 볼 수 있었던 여자선수에 대한 남성스태프의 성통제방법을 사회학적으로 고찰하는 것이다. 이를 위해 어빙 고프만의“지하생활(underlife)”개념을 사용해, K팀의 원정지에서의 생활을 구체적으로 기술하면서 팀의 질서와 선수의 성행위를 둘러싼 관계에 대해 논하려 한다. 특히 스태프들은 왜 선수의 성행위를 통제하려고 하는지, 또한 스태프와 선수의 성별이 다를 경우 그들은 어떻게 제재를 가하려고 하는지 그 메커니즘에 주목하여 기술하고자 한다. 그러기 위해 먼저 K팀 선수들의 성 정체성을 축으로 한 팀 내의 성별도식에 대해 설명하고, 거기서부터 형성된 선수들 사이의 관계성을 분석한다. 이를 근거로 스태프측과 선수측 각각이 지닌 원정생활의 의미에 대해서도 기술하려고 한다. 여기서 원정생활 숙소인 호텔의‘개인방’을 선수의 지하생활의 활동이 가능한 공간으로 간주한다. 왜냐하면 개인방에서는, 선수가 자신의 본래 역할과 거리를 둔 행위를 실천할 수 있기 때문이다. 결론으로 본 연구의 사례를 통해 알게 된 것은, K팀에서의 여성스태프란 여자선수를 신체적 정신적으로 지원하기 위해 필요한 존재임과 동시에 선수의 성행위를 보다 쉽게 통제하려는 남성스태프의 구체적인 계획하에 배치된 존재라는 점이었다. 이를 통해, 본 연구에서는‘선수의 성행위’가‘팀의 질서 유지’라는 명목하에 관리되고 있다는 점을 사회학적으로 시사하려 한다. The purpose of this research is to discuss the sexual behavior control method for female players by male staff in the sports team. With the case of the expedition life of Japanese women"s soccer K team as an example, this research studies the relationship between team"s order and players" sexual behavior, using the "underlife" concept of Erving Goffman. In that case, "the individual room" of the players in the expedition life is considered as the "underlife". This is because the individual room becomes a space in which player can take actions that are distanced from the original role of the player. Based on this, this paper will discuss why the staffs try to control the sexual behavior of the players and how the staffs deal with the players" sexual behavior if the sex of the player is different from the staff. Thus, this study will explain the gender diagram of the player by gender identity within the team, and this study will clarify the relationship between the players created from such a diagram, as well as the meaning of the expedition life for staffs and players. As a result, it is understood through the case of this paper that the female staff is a person who is required to not only simply support the female players physically and mentally, but also to make it easier to control the sexual behavior of the players within the team. From this research suggests sociologically how important the task of considering the relationship between "athlete"s sexual behavior" and "team order maintenance" is.

물푸레나무 수피의 성분연구

신은진, 류청, 황방연, 이미경 충북대학교 약품자원개발연구소 2010 약학논문집 Vol.25 No.-

Studies on the constituents of the stem barks of Fraxinus rhynchophylla(Oleaceae)led to the isolation of thirteen compounds. The structures of isolated compounds were identified on the basis of spectroscopic methods including 1D-NMR (¹H, ¹³C-NMR), 2D-NMR (COSY, HMQC, HMBC, ROESY) and MS, As a result, ten coumarin derivatives, esculetin ⑴, scopoletin ⑵, isoscopoletin ⑶, fraxetin ⑷, fraxidin ⑸, isofraxidin ⑹, 7,8-dimethoxy-6-hydroxycoumarin ⑺, esculin ⑻, fraxin ⑼, isofraxoside ⑽, one lignan, pinoresinol ⑾ and two phenolic compounds, caffeic acid ⑿ and 4-hydroxy-3-methoxy benzaldehyde ⒀ were isolated, Among them, fraxidin ⑹, 7,8-dimethoxy-6-hydroxycoumarin ⑺, isofraxoside ⑽ and pinoresinol ⑾ were first isolated from this plant.

Development of Novel Wastewater Treatment System by Sulfate Reducing Bacteria

Eunjin Lee,Yeo-Myeong Yun,Si-Kyung Cho,Jong-In Han,Kwiyong Kim,Hang-Sik Shin 한국폐기물자원순환학회 2013 한국폐기물자원순환학회 학술대회 Vol.2013 No.2

As an alternative for energy intensive aerobic wastewater treatment system, development of sulfate reducing bacteria (SRB)-based domestic wastewater treatment system was researched. No requirement of aeration, fast substrate utilization and less sensitiveness of SRB are main factors for energy conservative wastewater treatment system. Sulfate for SRB’s synthesis could be supplied from SOx in industrial gas, thus the system also can contribute to the economical treatment of hazardous sulfur compound in the gas. With the dissolved sulfate as an electron acceptor, SRB degrade organic matters in wastewater and produce sulfide. This produced sulfide was used for electricity generation in sulfide fuel cell system connected to continuously operating UASB reactor. This study also focused on obtaining SRB dominant granule from methanogen dominant anaerobic granule in a competition between SRB and methanogens. As a strategy to increase SRB activity and to suppress methanogens, starvation was applied by not providing organic source but continuous supplying sulfate. Based on the well adopted hypothesis on the granule structure, methanogens located relatively inner core of a granule died out due to the lack of organic source, while SRB located at outer part of granule survived utilizing extracellular polymeric substance (EPS) or organic matters from died out microbes as an organic source. By eliminating the competition, the portion of organic matter degradation by SRB was increased from 19% before starvation to 93% after starvation, achieving 80% COD removal in total. In case of sulfate removal, 15% improvement was achieved compared to the control reactor. This effect of starvation on the granule characteristics were confirmed with scanning electron microscope (SEM) and transmission electron microscope (TEM). Not only wastewater treatment by SRB but also electricity generation with sulfide fuel cell system was studied. Several sets of batch tests were conducted and continuous operation was also carried out with effluent containing 80mg/L -100mg/L sulfide. Through the batch tests, hydraulic retention time (HRT) was optimized to 8 h, removing almost all of the sulfide from the effluent, having the highest power density of 0.033 mW/cm². With the optimized HRT, sulfide fuel cell connected to the UASBr was continuously operated for over 50 days. This newly developed wastewater treatment system with SRB integrated with electricity generating sulfide fuel cell system is believed to be a promising technology for more sustainable wastewater treatment system.

교양교육에서의 블렌디드러닝 : 일본어수업의 성과와 전망

신은진 배재대학교 주시경교양교육연구소 2018 대학교양교육연구 Vol.3 No.1

This is a report on Blended learning practice in Japanese language classes in university liberal arts education. Conducted three years (2015, 2016 and 2018) using Blended learning with university LMS to perform Japanese language learning for Japanese learners with diverse backgrounds. At the beginning of the introduction, LMS had difficulty operating due to limitations on file upload capacity. After that, we used only three menus, [Announcement], [Master Data] and [Free Board], by dividing the number of menus used on-line. In addition, conversation and voice files were kept separately by teachers and shared feedback results with students. On the LMS, mainly the class material file (PPT) was uploaded, and the learner used it to facilitate the review and the test. As a result, the initial burden of Japanese learners as a liberal arts education decreased and the learning satisfaction improved, but the correlation between the execution of blended learning and learning satisfaction was not clear. Also, the [Free Board], which was held for mutual communication, did not receive a favorable response for three years. It is necessary to improve and supplement the "Blended learning" Japanese lesson design and operation. 본고는 대학교 교양 교육에서의 일본어 수업 블렌디드 러닝 실천보고이다. 다양한 배경을 가진 일본어 학습자를 대상으로 교양 일본어수업을 시행하기 위하여 3개년도(2015년도, 2016년도 및 2018년도)에 걸쳐 대학교 LMS를 활용하여 블렌디드 러닝을 실시하였다. 도입 초기에는 LMS의 파일 업로드 용량 제한 등으로 운영에 어려움이 있었으나 이후 온라인에서 사용하는 메뉴 수를 정제하여 [공지사항],[수업자료],[자유게시판]의 3종류만을 메뉴로 사용하였다. 또한 회화, 음성 파일 등은 교수자가 따로 보관하고 피드백 결과만을 공유하거나 LMS 상에는 주로 수업자료 파일(PPT)을 업로드하여 학습자의 복습 및 시험 대책이 용이하게 하였다. 그 결과, 교양 과목 일본어 학습자의 초기 부담도가 경감되고 학습만족도가 향상되었으나 블렌디드 러닝 시행과 학습만족도의 상관관계는 명확히 밝혀내지 못하였다. 또한 상호커뮤니케이션을 활성화하기 위하여 개설한 [자유게시판]은 3개년도 모두 호응을 얻지 못하였다. 향후 블렌디드 러닝 일본어 수업 설계 및 운영에 있어서 개선 보완이 필요하다 하겠다.