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페닐부타존에 의해 간손상이 유발된 생쥐의 유전자 발현 분석
이은주(Eun-Ju Lee),정인해(In-Hye Jeong),김한나(Han-Na Kim),정희경(Heekyoung Chung),공구(Gu Kong),강경선(Kyung-Sun Kang),윤병일(Byung-IL Yoon),이병훈(Byeong Hoon Lee),이미옥(Mi-Ock Lee),김주한(Ju Han Kim),김형래(Hyung-Lae Kim) 한국독성학회 2006 Toxicological Research Vol.22 No.2
The KFDA (Korea Food & Drug Administration) has performed a collaborative toxicogenomics project since 2003. Its aim is to construct a toxicologenomic database of 12 hepatotoxic compounds from mice livers. Phenylbutazone which is non-steroidal anti-inflammatory drug was assigned. It was administered at low (0.0238 ㎎/㎏) and at high (0.238 ㎎/㎏) dose (5 mice per group) orally to the postnatal 6 weeks ICR mice, then the serum and liver were collected at the indicated time (6, 24 and 72 h) after administration. Serum biochemical markers for liver toxicity were measured and histopathologic studies also were carried out. The gene expression profiling was carried out by using Applied Biosystems 1700 Full Genome Expression Mouse. The 2-way ANOVA was used to find genes that reflected phenylbutazone-induced acute toxicity or dose-dependant changes. By self-organization maps (SOM), we identified groups with unique gene expression patterns, some of them are supposed to be related to phenylbutazone induced toxicity, including lipid metabolism abnormality, oxidative stress, cell death and cytoskeleton destruction.
공은숙(Eun Suk Kong),김은주(Eun-ju Kim) 한국콘텐츠학회 2014 한국콘텐츠학회논문지 Vol.14 No.9
본 연구는 통합적 인지훈련프로그램이 지역사회 노인의 인지기능에 미치는 효과를 파악하는데 목적이 있다. 연구방법은 비동등성 대조군 전후설계로 일 지역의 노인복지관을 이용하는 만 65세 이상 노인 중 56명을 실헙군과 대조군에 각각 28명씩 임의 배정하였으며, 실험군에는 2011년 9월 3주부터 12월 2주까지 1주2회, 1회 90분씩, 12주간, 총 24회의 통합적 인지훈련프로그램을 적용하였다. 실험처치 전에 실험군과 대조군의 인적사항과 노인용인지검사를 실시하였으며, 실험처치 후에 다시 실험군과 대조군의 노인용인지검사를 실시하여 자료를 수집하였다. 연구결과, 통합적 인지훈련프로그램 실시 후 실험군의 노인용 인지검사(전체지능, 기초지능, 관리지능, 주의기능, 작업기억, 언어기억, 시공간력, 기억기능, MMSE-K1)점수가 대조군에 비해 통계적으로 유의하게 상승된 것으로 나타났다(p< .05). 본 연구는 통합적 인지훈련 프로그램이 지역사회 노인의 인지기능을 향상시킬 수 있다는 점을 파악한 데 의의가 있다. The purpose of this study is to investigate the Effects of the Integrative Cognitive Training Program on Cognitive Function of the community elderly. Nonequivalent control group pretest-posttest design was used. 28 subjects were assigned to each of the group. 24 sessions of the integrative Cognitive Training Program were applied to the experimental group from September to December. The program was provided twice per week for 12weeks, total 24 sessons to the experimental group. Cognition scale for older adults(CSOA) was used to measure cognitive function of the groups before and after the Intervention. The score of Cognition Scale for Older Adults was significantly increased in experimental group than in control group(p< .05). The results of the study that Integrative Cognitive Training Program can be vey useful to improve the cognitive ability for the community elderly.
교통장애인의 자기효능감과 장애수용이 사회통합에 미치는 영향 : 재활동기의 매개효과를 중심으로
공마리아,최은영,신주영 대구대학교 특수교육재활과학연구소 2015 특수교육재활과학연구 Vol.54 No.2
본 연구는 교통장애인의 자기효능감과 장애수용이 사회통합에 미치는 영향에 있어 재활동기의 매개효과를 살펴보기 위한 것이다. 이를 위해 경상북도 지역의 교통장애인협회에 가입되어 있는 교통장애인 306 명을 대상으로 설문을 실시하고,최종 253부를 분석하였다. 연구 결과 교통장애인의 자기효능감은 사회통합에 직접적인 영향을 미치는 것으로 나타났으나,교통장애인의 장애수용은 사회통합에 직접적인 영향을 미치지 않는 것으로 확인되었다. 또한 교통장애인의 재활동기는 자기효능감 및 장애수용과 사회통합의 관계에서 매개효과를 지님을 확인하였다. 이를 토대로 교통장애인의 사회통합을 중진시키기 위해서는 자기효능감과 장애 수용 뿐만 아니라 재활동기를 향상시키기 위한 프로그램을 개발할 필요가 있음을 제안하였다. The aim of this study is to investigate the mediating effect of motivation for rehabilitation in relation to effects of self-efficacy and disability acceptance on social integration in people with traffic-related disabilities. Survey questionnaires were distributed to the members of Korean Traffic Disabled Association who had traffic related disability. 306 questionnaires were returned and data from 253 questionnaires were used for analysis. The results indicated that participants’ level of self-efficacy directly influences their level of social integration. However, the results did not indicate that the level of disability acceptance directly influences their social integration. Also, they showed motivation for rehabilitation's mediating effect on the relationships between participants' level of sell-efficacy, disability acceptance and social integration. In this vein, it would be effective to develop interventions that focus on improving their motivation for rehabilitation in enhancing their social integration.
Ju, Bong-Gun,Solum, Derek,Song, Eun Joo,Lee, Kong-Joo,Rose, David W.,Glass, Christopher K.,Rosenfeld, Michael G. Elsevier 2004 Cell Vol.119 No.6
<P><B>Abstract</B></P><P>Switching specific patterns of gene repression and activation in response to precise temporal/spatial signals is critical for normal development. Here we report a pathway in which induction of CaMKIIδ triggers an unexpected switch in the function of the HES1 transcription factor from a TLE-dependent repressor to an activator required for neuronal differentiation. These events are based on activation of the poly(ADP-ribose) polymerase1 (PARP-1) sensor component of the groucho/TLE-corepressor complex mediating dismissal of the corepressor complex from HES1-regulated promoters. In parallel, CaMKIIδ mediates a required phosphorylation of HES1 to permit neurogenic gene activation, revealing the ability of a specific signaling pathway to modulate both the derepression and the subsequent coactivator recruitment events required for transcriptional activation of a neurogenic program. The identification of PARP-1 as a regulated promoter-specific exchange factor required for activation of specific neurogenic gene programs is likely to be prototypic of similar molecular mechanisms.</P>
Ju-Won Kim,Ja Young Cho,Dong-Gyun Kim,Bo-Hye Nam,Eun-Soo Nho,Bong-Seok Kim,Young-Ok Kim,Hee Jeong Kong 한국발생생물학회 2020 발생과 생식 Vol.24 No.3
Primary cell culture is a sufficient method frequently used to study the cellular properties and mechanisms of isolated cells in a controlled environment. In this study, an embryonic cell line (FGBC8) derived from the blastula stages of embryos of olive flounder Paralichthys olivaceus was developed. Furthermore, conditions for optimal long-term maintenance of this primary embryonic cell culture were investigated. Morphologically, FGBC8 cells were composed primarily of epithelial-like cells. FGBC8 cells were subcultured for >160 passages over ~830 days. The doubling time of FGBC8 cells was 73.8 h, and the modal diploid chromosome number was 48. FGBC8 cells transfected with green fluorescence protein (GFP)-expression plasmid exhibited a strong signal 48 h after transfection. Consequently, we demonstrated that fish serum is a crucial supplement for the long-term survival and maintenance of comparable morphology in these primary embryonic cells. Our results can be used as a guide for primary embryonic cell cultures for other fish species and may be useful for cell biotechnological applications.