http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
음식물 폐기물류로 제조된 퇴비의 고형연료 가치 평가 연구
이경섭,장한별,정은지,도아름,하진욱 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2
In 2019, the daily generation of food waste in Korea was 14,314 tons, which is a steady increase compared to the past. To efficiently use food waste as an effective resource, composting and feeding are carried out. In Korea, attention is gradually focused on a sustainable resource circulation society. Compost made of food waste has problems with foreign substances, heavy metals, and salt content, and solid fuelization research is being actively conducted to compensate for this. In this study, infrared spectroscopy(FT-IR), organic content measurement(TGA), and chemical composition were measured to compare the fuel characteristics with wood pellets, which are major solid fuels, in order to examine the physical and chemical properties of food waste compost.
Lee, Janghyun,Park, Eun-Byeol,Min, Jiyoun,Sung, Si-Eun,Jang, Yejin,Shin, Jin Soo,Chun, Dongmin,Kim, Ki-Hun,Hwang, Jihyun,Lee, Mi-Kyung,Go, Yun Young,Kwon, Dohyeong,Kim, Meehyein,Kang, Suk-Jo,Choi, Byo Oxford University Press 2018 Nucleic acids research Vol.46 No.4
<P><B>Abstract</B></P><P>Retinoic acid-inducible gene I (RIG-I) recognizes double-stranded viral RNAs (dsRNAs) containing two or three 5′ phosphates. A few reports of 5′-PPP-independent RIG-I agonists have emerged, but little is known about the molecular principles underlying their recognition. We recently found that the bent duplex RNA from the influenza A panhandle promoter activates RIG-I even in the absence of a 5′-triphosphate moiety. Here, we report that non-canonical synthetic RNA oligonucleotides containing G-U wobble base pairs that form a bent helix can exert RIG-I-mediated antiviral and anti-tumor effects in a sequence- and site-dependent manner. We present synthetic RNAs that have been systematically modified to enhance their efficacy and we outline the basic principles for engineering RIG-I agonists applicable to immunotherapy.</P>
Kinesin-1-dependent transport of the βPIX/GIT complex in neuronal cells
( Eun-young Shin ),( Chan-soo Lee ),( Han-byeol Kim ),( Jin-hee Park ),( Kwangseok Oh ),( Gun-wu Lee ),( Eun-yul Cho ),( Hyong Kyu Kim ),( Eung-gook Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2021 BMB Reports Vol.54 No.7
Proper targeting of the βPAK-interacting exchange factor (βPIX)/G protein-coupled receptor kinase-interacting target protein (GIT) complex into distinct cellular compartments is essential for its diverse functions including neurite extension and synaptogenesis. However, the mechanism for translocation of this complex is still unknown. In the present study, we reported that the conventional kinesin, called kinesin-1, can transport the βPIX/GIT complex. Additionally, βPIX bind to KIF5A, a neuronal isoform of kinesin-1 heavy chain, but not KIF1 and KIF3. Mapping analysis revealed that the tail of KIF5s and LZ domain of βPIX were the respective binding domains. Silencing KIF5A or the expression of a variety of mutant forms of KIF5A inhibited βPIX targeting the neurite tips in PC12 cells. Furthermore, truncated mutants of βPIX without LZ domain did not interact with KIF5A, and were unable to target the neurite tips in PC12 cells. These results defined kinesin-1 as a motor protein of βPIX, and may provide new insights into βPIX/GIT complex-dependent neuronal pathophysiology. [BMB Reports 2021; 54(7): 380-385]
Clinical and Radiological Features of Korean Patients With Anti-HMGCR Myopathy
Eun Kyoung Oh,Seung-Ah Lee,Hyun Joon Lee,Yoon Jin Cha,Sungjun Kim,Hyung-Soo Lee,Bum Chun Suh,Ha Young Shin,Seung Woo Kim,Byeol-A Yoon,Seong-il Oh,Yoo Hwan Kim,Joong-Yang Cho,Jeong Hee Cho,Ki-Han Kwon 대한신경과학회 2023 Journal of Clinical Neurology Vol.19 No.5
Background and Purpose To understand the characteristics of Korean patients with anti- 3-hydroxy-3-methylglutaryl-coenxyme A reductase (HMGCR) myopathy, we measured anti- HMGCR antibodies and analyzed the clinical, radiological, and pathological features of patients with anti-HMGCR myopathy. Methods We measured titers of anti-HMGCR antibodies in the sera of 99 patients with inflammatory myopathy, 36 patients with genetic myopathy, and 63 healthy subjects using an enzyme-linked immunosorbent assay. We tested 16 myositis-specific autoantibodies (MSAs) in all patients with anti-HMGCR myopathy. Results Positivity for the anti-HMGCR antibody was observed in 17 (4 males and 13 females) of 99 patients with inflammatory myopathy. The median age at symptom onset was 60 years. Ten (59%) of the patients with anti-HMGCR positivity had taken statins. The titer of anti- HMGCR antibodies was significantly higher in the statin-naïve group (median=230 U/mL, interquartile range=170–443 U/mL) than in the statin-exposed group (median=178 U/mL, interquartile range=105–210 U/mL, p=0.045). The most common symptom was proximal muscle weakness in 15 patients (88%), followed by myalgia in 9 (53%), neck weakness in 4 (24%), dysphagia in 3 (18%), and skin lesions in 2 (12%). The median titer of anti-HMGCR antibody was 202 U/mL. We found eight different MSAs in nine (53%) patients. The median disease duration from symptom onset to diagnosis was significantly shorter in the MSA-positive group than in the MSA-negative group (p=0.027). Conclusions Our study was the first to measure anti-HMGCR antibodies in inflammatory myopathy. It has provided new findings, including the suggestion of the coexistence of other MSAs in Korean patients.
The Effects of Samultang-ga-dansam for Wound Healing
( Lee Eun-byeol ) 대구한의대학교 제한동의학술원 2022 제한동의학술원논문집 Vol.20 No.1
Objectives : The purpose of this study was to evaluate the antioxidant, anti-inflammatory and wound healing effects of Samultang-ga-dansam water extract (SD) in wound-induced mice. Methods : The mice were divided into five groups (n = 7): the normal group, the control group, the positive control group, the low-dose SD group and the high-dose SD group. The normal group had no wounds and the remaining groups were wounded on the back with a leather punch. Distilled water was administered to the control group, 200 mg/kg of Vitamin E was administered to the positive control group. In the low-dose SD group and the high-dose SD group, 1.23 g/kg and 2.47 g/kg of SD were administered, respectively. In order to confirm the antioxidant effect, the total content of polyphenols and flavonoids and the scavenging activity of DPPH and ABTS free radicals were measured. ROS, ONOO<sup>-</sup> and pro-inflammatory cytokines in the serum were measured to confirm the antioxidant and anti-inflammatory effect. Furthermore, the levels of NF-B p65, iNOS, COX-2, TNF-α, IL-1β and IL-6 involved in the inflammatory response, as well as the expression of Nrf2, Keap1 and NADPH oxidases related to antioxidants, were measured using western blot analysis. AST and ALT in serum were measured to check risk of liver injury. a-SMA and COL1A1 in skin tissue were measured to determine the degree of connective tissue recovery. The wound site was visually observed and histologically analyzed to confirm effects of SD for wound healing. Results : The total polyphenol content of the SD was 26.59 ± 0.20 mg (GAE)/g and the total flavonoid was 3.04 ± 0.27 mg (QE)/g. The IC<sub>50</sub> values in DPPH and ABTS were 37.39 ± 0.89 and 105.81 ± 0.54 ㎍/㎖, respectively. Oral administration of the SD significantly reduced the visible skin damage and decreased the ROS and ONOO<sup>-</sup> activity of the serum. It significantly increased HO-1, SOD, catalase, GPx-1/2, Nrf2 and Keap-1 which are antioxidant-related factors in skin tissue and reduced NF-κB p65, iNOS, COX-2, TNF-α, IL-1β, IL-6 which are inflammation-related factors. Also, SD significantly decreased NOX2, p22<sup>phox</sup> and p47<sup>phox</sup> and increased α-SMA and COL1A1 protein expression in fibroblasts involved in connective tissue repair. Both AST and ALT did not show a significant difference from the control group, confirming the safety of liver injury. According to histological examination, the thickened epithelial layer was thinned and collagen fibers were increased to accelerate wound healing. Conclusions : It is suggested that Samultang-ga-dansam has antioxidant and anti-inflammatory effects and promotes wound tissue repair.
Lee, Eun Byeol,Kim, Jun Hyeong,An, Chang Wan,Kim, Yeong Jee,Noh, Yun Jeong,Kim, Su Jin,Kim, Ju-Eun,Shrestha, Abinash Chandra,Ham, Ha-Neul,Leem, Jae-Yoon,Jo, Hyung-Kwon,Kim, Dae-Sung,Moon, Kwang Hyun,L The Korean Society of Applied Pharmacology 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.6
In order to discover lifespan-extending compounds made from natural resources, activity-guided fractionation of Zingiber officinale Roscoe (Zingiberaceae) ethanol extract was performed using the Caenorhabditis elegans (C. elegans) model system. The compound 6-gingerol was isolated from the most active ethyl acetate soluble fraction, and showed potent longevity-promoting activity. It also elevated the survival rate of worms against stressful environment including thermal, osmotic, and oxidative conditions. Additionally, 6-gingerol elevated the antioxidant enzyme activities of C. elegans, and showed a dose-depend reduction of intracellular reactive oxygen species (ROS) accumulation in worms. Further studies demonstrated that the increased stress tolerance of 6-gingerol-mediated worms could result from the promotion of stress resistance proteins such as heat shock protein (HSP-16.2) and superoxide dismutase (SOD-3). The lipofuscin levels in 6-gingerol treated intestinal worms were decreased in comparison to the control group. No significant 6-gingerol-related changes, including growth, food intake, reproduction, and movement were noted. These results suggest that 6-gingerol exerted longevity-promoting activities independently of these factors and could extend the human lifespan.