Intron 4 VNTR (4a/b) Polymorphism of the Endothelial Nitric Oxide Synthase Gene Is Associated with Breast Cancer in Mexican Women

Ramírez-Patiño Ramiro,Figuera Luis Eduardo,Puebla-Pérez Ana María,Delgado-Saucedo Jorge Ivan,Legazpi-Macias María Magdalena,Mariaud-Schmidt Rocio Patricia,Ramos-Silva Adriana,Gutiérrez-Hurtado Itzae A 대한의학회 2013 Journal of Korean medical science Vol.28 No.11

The endothelial nitric oxide synthase (eNOS) gene plays an important role in several biological functions. Polymorphisms of the eNOS gene have been associated with cancer. It has been suggested that the VNTR 4 a/b polymorphism may affect the expression of eNOS and contributes to tumor promotion in the mammary gland. We examined the role of the eNOS4 a/b polymorphism by comparing the genotypes of 281 healthy Mexican women with the genotypes of 429 Mexican women with breast cancer (BC). The observed genotype frequencies for control and BC patients were 0.6% and 0.7% for a/a (polymorphic); 87% and 77% for a/a (wild type); and 12% and 22% for a/b respectively. We found that the odds ratio (OR) was 1.9, with a 95% confidence interval (95%CI) of 1.29-2.95, P=0.001 for genotypes a/a-a/b, b/c. The association was also evident when comparing the distribution of the a/a-a/b genotypes in patients with high levels of glutamate-oxaloacetate transaminase (SGOT) (OR, 1.93; 95% CI, 1.14-3.28; P=0.015); undergoing menopause with high levels of SGOT (OR, 2.0; 95% CI, 1.1-3.84); and with high levels of glutamic-pyruvic transaminase (SGPT) (OR, 3.5; 95% CI, 1.56-8.22). The genotypes a/a-a/b are associated with BC susceptibility in the analyzed samples from the Mexican population.

AURKA Gene Variants rs1047972, and rs8173 Are Associated With Breast Cancer

Eric Jonathan Maciel-Cruz,Luis Eduardo Figuera-Villanueva,Asbiel Felipe Garibaldi-Ríos,Belinda Claudia Gómez-Meda,Guillermo Moisés Zúñiga-González,Ana María Pérez,Paola B Castro-García,Ramiro Ramírez- 한국유방암학회 2023 Journal of breast cancer Vol.26 No.4

Purpose: Association between variants rs1047972 and rs8173 of the AURKA gene in healthy women and breast cancer (BC) in a Mexican population. Methods: Genomic DNA samples from 409 healthy women and 572 patients with BC were analyzed for variants rs1047972 and rs8173 of the AURKA gene by polymerase chain reaction-restriction fragment length polymorphism. Results: TT genotype (odds ratio [OR], 2.5; 95% confidence interval [CI], 1.22–5.11; p = 0.0015) and the T allele (OR, 1.16; 95% CI, 1.23–2.12; p = 0.0007) of the rs1047972 variant were associated as risk susceptibility for BC relative to the control group. Contrarily, the GG genotype (OR, 0.64; 95% CI, 0.43–0.94; p = 0.029) was associated as a protective factor of susceptibility of BC of the variant rs8173 of the AURKA gene. Differences were observed in the patients with BC who were carriers of the CT genotype of the rs1047972 variant with overweight, obesity, estrogen receptor-positive plus obesity, Ki-67 (≥ 20%) plus history familial positive of cancer; and for variant rs8173 the BC patients who were CG carriers and presented chemotherapy gastric toxicity, hormonal receptor positive plus chemotherapy gastric toxicity, and menopause status plus chemotherapy gastric toxicity (p < 0.05). Two common haplotypes were identified in the study groups: CG and TC genotypes, were associated as a protective and risk factor, respectively (p < 0.05). Conclusion: Variants rs1047972 and rs8173 of the AURKA gene and the TC haplotype were associated as risk susceptibility factors for BC in this population.

Relaxant and anti-inflammatory effect of two thalidomide analogs as PDE-4 inhibitors in pregnant rat uterus

Víctor Manuel Muñoz-Pérez,Eduardo Fernández-Martínez,Héctor Ponce-Monter,Mario I. Ortiz 대한약리학회 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.4

The aim of this study was to evaluate the relaxant and anti-inflammatory effects of two thalidomide analogs as phosphodiesterase-4 (PDE-4) inhibitors in pregnant rat uterus. Uteri from Wistar female rats were isolated at 19 day of pregnancy. Uterine samples were used in functional studies to evaluate the inhibitory effects of the thalidomide analogs, methyl 3-(4-nitrophthalimido)-3-(3,4- dimethoxyphenyl)-propanoate (4NO2PDPMe) and methyl 3-(4-aminophthalimido)- 3-(3,4-dimethoxyphenyl)-propanoate (4APDPMe), on prostaglandin-F2α (PGF2α)- induced phasic, K+-induced tonic, and Ca2+-induced contractions. Accumulation of cAMP was quantified in uterine homogenates by ELISA. Anti-inflammatory effect was assessed by using ELISA for determination of the pro-inflammatory cytokines tumor necrosis factor-α (TNFα) and interleukin (IL)-1β, and anti-inflammatory IL-10, from uterine explants stimulated with lipopolysaccharide (LPS). Nifedipine, forskolin and rolipram were used as positive controls where required. Both thalidomide analogs induced a significant inhibition of the uterine contractions induced by the pharmaco- and electro-mechanic stimuli. Nifedipine and forskolin were more potent than the analogs to inhibit the uterine contractility, but these were more potent than rolipram, and 4APDPMe was equieffective to nifedipine. Thalidomide analogs increased uterine cAMP-levels in a concentration-dependent manner. The LPS-induced TNFα and IL-1β uterine secretion was diminished in a concentrationdependent fashion by both analogs, whereas IL-10 secretion was increased significantly. The thalidomide analogs induced utero-relaxant and anti-inflammatory effects, which were associated with the increased cAMP levels as PDE-4 inhibitors in the pregnant rat uterus. Such properties place these thalidomide analogs as potentially safe and effective tocolytic agents in a field that urgently needs improved pharmacological treatments, as in cases of preterm labor.

Catalytic hydrothermal conversion of CO2 captured by ammonia into formate using aluminum-sourced hydrogen at mild reaction conditions

Juan I. del Río,Eduardo Pérez,David León,Ángel Martín,María D. Bermejo 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.97 No.-

The catalytic conversion of CO2 captured in aqueous media into formate was studied using aluminum-sourced hydrogenin a batch reaction system. To do so, themain ammonia-basedCO2absorption derivatives:ammoniumcarbamate,carbonate and bicarbonateandsodiumbicarbonatewere selectedasCO2source.Theperformance of the different species was determined under mild hydrothermal reaction conditions (120 C),using Pd/C 5 wt% catalyst. In these conditions, the formate yield and selectivity increase in the orderammonium bicarbonate < sodium bicarbonate < ammonium carbonate < ammonium carbamate. Ammo-nium bicarbonate and sodium bicarbonate reagents needed higher temperature (250 C) for an increasedyield. Results with ammonium carbamate as starting material indicate a significant effect of time andcatalyst content on formate yield, which ranged between 4 and 38%. Experiments with gaseous H2 showedthat a comparable yield with Al can be obtained at a similar level of pressure. The reutilization andcharacterization of the reaction solid, comprising exhausted aluminum and Pd/C catalyst, showed that thealuminum was not completely oxidized up to the 5th re-use, and Pd can play a reducing role through theformation of palladium hydride species. The process can be improved by operating at higher pressure andlower temperature, to avoid loss of yield by dehydration of formate.

Effect of Ditaxin and Heteranthin and Inhibitory Effect of Ditaxis heterantha Extract on L5178Y Tumor Development in Mice

María Eugenia Jaramillo-Flores,Herry Heriyati Permady,Ana Maria Puebla-Pérez,Eduardo Padilla,Eugenia del Carmen Lugo-Cervantes,Jorge Ivan Delgado-Saucedo,Eva Ramón-Gallegos 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.5

Ditaxis heterantha seeds are used as spices for flavoring and coloring food. Two new apocarotenoids derived from the seeds, heteranthin and ditaxin, were evaluated for their in vitro cytotoxic effects in murine lymphoma cells lines. Bioabsorption in mice and preventive and antitumor effects of the apocarotenoids were determined. Ditaxin and heteranthin showed cytotoxic effects in vitro against murine malignant cells and normal splenocyte cells. The 50% inhibitory concentration (IC_50) for ditaxin in splenocytes was 0.1825 mM; in L5178Y, the IC_50 was 0.1923 mM. The heteranthin IC_50 in splenocytes was 0.1325 mM; in L5178Y, the value was 0.3889 mM. The maximum ditaxin plasma concentration was found after 2 hours of administration (mean±standard deviation, 7.5±2.05 μg/mL). Oral administration of the D. heterantha extract (100 mg/kg per day) for 14 days after the L5178Y lymphoma cell implantation showed no significant effect compared with groups that were not pretreated. However, tumor inhibition in groups treated intraperitoneally before inoculation with the L5178Y cells showed a significant difference (P<.001) compared with the groups not pretreated.